LODZ PLATELET CONFERENCE jointly with

11th Erfurt Conference on Platelets June 25th – 28th, 2006

Lodz, Poland

Organized on behalf of the

European Platelet Group (EPG)

Technical University of Lodz (TUL)

University of Lodz (UL)

Medical University of Lodz (UMED)

Commission of Haemostasis and Thrombosis, the Committee

of Pathophysiology, Polish Academy of Sciences

www.p.lodz.pl/plateletconference

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Scientific Committee

Prof. Włodzimierz Buczko - Medical University of Bialystok

Prof. Czesław Cierniewski - Medical University of Lodz

Prof. Ryszard Gryglewski - Jagiellonian University Medical College

Prof. Stan Heptinstall - Medicine University Hospital Nottingham

Prof. Michał Myśliwiec - Medical University of Bialystok

Prof. Peter Spangenberg - University of Applied Sciences, Jena

Prof. Andrzej Szczeklik - Jagiellonian University Medical College

Prof. Krzysztof Warzocha - Institute of Hematology and Transfusiology, Warsaw

Prof. Marek Wojtukiewicz - Medical University of Bialystok

Organizing Committee

Bogdan Walkowiak - Chairman

Wolfgang Loesche Urszula Kralisz Zofia Pawłowska Barbara Wachowicz Cezary Watała

Witold Szymański - chief of the secretariat Marta Kamińska - secretary

Dorota Ciepielewska - secretary Witold Jakubowski - www page Piotr Niedzielski - treasurer

Bogdan Kontek Jan Szemraj

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The organizers thank the following who are among those

sponsoring the Conference:

Dade Behring Diagnostics Sp zoo (Poland)

Dade Behring Marburg (Germany)

Wyeth Pharma Münster (Germany)

Branch of Warsaw

SanLab S.C.

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PROGRAMME Sunday, June 25th, 2006 9:00 – 17:00 Registration level 0 18:00 – 18:10 Welcome Message level –1 18:10 – 18:55 Opening lecture Gustav Born (U.K.) Current questions from earlier answers 19:00 – 20:30 Welcome Reception level –1 Monday, June 26th, 2006 Session I Chairmen: Giovanni De Gaetano and Cezary Watala 9:00 – 9:55 Invited lecture I level –1 Jan W Akkerman (The Netherlands) Insulin signalling in Diabetes mellitus type 2 platelets 9:55 – 10:25 Oral presentations 1-2 1. R.A.Claus, C.L.Bockmeyer, U.Budde, R.Schneppenheim, K.Kentouche, M.Bauer, K.Reinhart, F.M.Brunkhorst, W.Lösche Decrease of ADAMTS13 activity is a common feature of the inflammatory response (15 minutes) 2. A.Michno, A.Szutowicz The modification of blood platelet activity by acetyl-CoA and ATP/ADP in diabetic platelets (15 minutes)

10:25 – 10:50 Coffee break Session II Chairmen: Maria Zellner and Peter Spangenberg 10:50 – 11:50 Oral presentations 3-7 level –1 4. P.Järemo, C.Starkhammar, M.Milovanovic, Å.Lundström, T.L.Lindahl, N.Ravald, A.Richter Inverse associations between platelet reactivity and the severity of gingivitis in stable angina pectoris (15 minutes) 5. S.Jones, G.Graham, T.Sage, J.Gibbins Peripheral tachykinins stimulate activatory signaling pathways in platelets (15 minutes) 6. P.Bugert, A.Schedel, P.Schloss, H.Klüter The dopaminergic system of human platelets (15 minutes)

7. P.Manolopoulos, S.Heptinstall, J.R.Glenn, S.C.Fox, J.A.May, N.Dovlatova, S-W.Tang, N.Bauer, M.Hesse, N.R.Thomas, V.Ralevic Acyl derivatives of coenzyme a inhibit ADP-induced platelet function via antagonism at P2Y1 and P2Y12 receptors (15 minutes) 11:50 – 14:00 Lunch

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Session III Chairmen: Jan W. Akkerman and Czeslaw S. Cierniewski 14:00 – 14:55 Invited lecture II level –1 Czeslaw S. Cierniewski (Poland) Platelet microparticles and angiogenesis 14:55 – 15:50 Invited lecture III level –1 Elizabeth Kornecki (USA) Role for the F11 Receptor (F11R; JAM-A) in hemostasis, inflammatory thrombosis and atherosclerosis

15:50 – 16:15 Coffee break Session IV Chairmen: Patricia Maguire and Wolfgang Lösche 16:15 – 17:00 Oral presentations 8-10 level –1 8. R.A.Claus, C.L.Bockmeyer, S.Reiss, R.Schneppenheim, W.Lösche, V.Oberle Direct interaction of platelet derived microvesicels with Von Willebrand factor: a microscopic study (15 minutes) 9. R.Polanowska-Grabowska, R.A.Figler, M.A.Marshall, K.Wallace, A.R.L.Gear, J.Linden Blood platelet activation in a transgenic mouse model of sickle cell disease (15 minutes) 10. M.Boncler, M.Nocuń, P.Gresner, R.Wilk, J.Rysz, C.Watala Acetylation of platelet proteins by acetylsalicylic acid is decreased in hypercholesterolemic patients (15 minutes) 18:30 - Conference dinner - the Biederman Palace Tuesday, June 27th, 2006 Session V Chairmen: Elizabeth Kornecki and Włodzimierz Buczko 9:00 - 9:55 Invited lecture IV level –1 Giovanni de Gaetano (Italy) Should we forget about platelets and move to leucocytes? 9:55 – 10:50 Invited lecture V Stan Heptinstall (U.K.) Leukocytes influence platelet aggregation more than you think 10:50 – 11:15 Coffee break Session VI Chairmen: Barbara Wachowicz and Peter Presek 11:15 – 13:00 Oral presentations 11-17 level –1 11. V.Oberle, R.Rüger, A. Fahr, W.Lösche Negatively charged liposomes used for drug delivery bind to platelets and leukocytes and activate them (15 minutes) 12. S.C.Fox, J.A.May, S.Heptinstall Quantitation of endothelial microparticles in plasma samples is impaired by chylomicrons following ingestion of a fatty meal (15 minutes)

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13. M.Milovanovic, L.Kourosh, T.L.Lindahl, C.Hallert, P.Järemo

Platelet density variations in essential thrombocytemia (15 minutes) 14. C.Watala, R.Golanski, W.Kuliczkowski, P.Gresner, J.Golanski

More on aspirin resistance: position paper of the working group on aspirin resistance. The next opening. Our proposal for a laboratory test of “aspirin resistance” (15 minutes) 15. W.Kuliczkowski, J.Golanski, R.Golanski, S.Chlopicki, J.Baraniak, C.Watala Acetylsalicylic acid non-responsiveness after coronary artery bypass grafting surgery results in higher TXB2 generation under shear stressconditions (15 minutes) 16. A.Schaarschmidt, J.Konter, S.Reiss, V.Oberle, R.A.Claus, E.Glusa, J.Lehmann, W.Lösche Inhibition of platelet aggregation by 4-methyl piperidine NONOate is mediated by plasma factors but not by cGMP (15 minutes) 17. A.Juška, I.Jardin, G.M.Salido, J.A.Rosado

Functional characteristics of two types of calcium stores and SERCAs in human platelets (15 minutes) 13:00 – 15:00 Lunch Session VII Chairmen: Janusz Szemraj and Marek Wojtukiewicz 15:00 – 17:00 Poster session level -1 p 01. J.Saluk-Juszczak, B.Olas, P.Nowak, B.Wachowicz Antioxidative effects of D-glucaro 1,4-lactone against peroxynitrite - induced modifications in blood platelet p 02. B.Olas, J.Saluk-Juszczak, I.Pawlaczyk, P.Nowak, J.Kołodziejczyk, R.Gancarz, B.Wachowicz Response of blood platelets to an extract from Conyza canadensis in vitro p 03. T.Domaniewski, A.Karp, A.Mogielnicki, E.Chabielska, W.Buczko The effect of ACE-Is on platelet adhesion and aggregation in rats p 04. C.L.Bockmeyer, W.Lösche, V.Oberle, A.Helbig, N.Kretschmar, T.Hilberg, R.A.Claus Physical stress as a model to study variations in ADAMTS13 activity; Von Willebrand factor and platelet activation p 05. K.Bednarska, A.Olejniczak, B.A.Wojtczak, Z.Sułowska, Z.Leśnikowski Adenosine modified with boron cluster pharmacophore as new potent platelet inhibitors p 06. K.Bednarska, A.Schromm, T.Cieślikowski, A.St.Świerzko, W.Kaca, Z.Sułowska

Evidence for direct regulatory interactions between lipopolysaccharide, thrombin and platelets: differential effects of LPS from Escherichia coli and Proteus mirabilis on thrombin activity and platelet activation p 07. K.Fälker, D.Lange, P. Presek Signaling pathways mediated by the P2Y12 ADP receptor upon thrombin stimulation in human platelets p 08. A.Dietrich-Muszalska, B.Kontek Generation of superoxide anion in blood platelets of patients with schizophrenia of paranoid type

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p 09. A.White, J.Glenn, S.Fox, A.Johnson, B.Myers, S.Heptinstall Reduced platelet aggregation to ADP and ATP in patients with leucocytosis is due to enhanced rates of adenine nucleotide metabolism mediated by the NTPDase CD39 p 10. K.Oszajca, B.Walkowiak, E.Chabielska, J.Bartkowiak, J.Szemraj A new thrombolytic, antiplatelet agent with higher fibrin affinity – a staphylokinase variant p 11. V.Bichan, A.Samal, Z.Zabarovskaya Ca+ signalling in pregnant women with gestational diabetes mellitus p 12. E.Nita, L.Konopka, K.Bykowska, K.Maślanka, S.Łopaciuk, Z.Traczyk, R.Woroniecka, B.Pieńkowska-Grela

Bernard-Solulier syndrome in three siblings with a supernummerary small marker chromosome p 13. M.L.Udvardy, J.Kappelmayer, J.Hársfalvi Glycocalicin coated beada for studyind platelet GPIB function p 14. B.Nagy Jr, É.Csongrádi, I.Balogh, M.Káplár, G.Paragh, J.Kappelmayer Evaluation of increased Platelet activation and the effect of P-selectin gene (T715P) polymorphism in type 2 diabetes mellitus and obesity p 15. M.Rozalski, B.Luzak, J.Rywaniak, S.Rozalska, T.Poplawski, R.Sychowski, C.Watala

Genotyping the common polymorphisms of platelet purinoreceptors. Methodological aspects p 16. M.Zellner, M.Diestinger, E.Rappold, W.Winkler, F.Garo, R.Oehler Platelet proteome profiling from dementia patients p 17. B.Walkowiak, M.Kamińska, W.Okrój, W.Tanski, A.Sobol, Z.Zbróg, I.Przybyszewska-Doroś Blood platelet proteome is changed in uremic patients p 18. L.Michalec, K.Błędzka, J.Szymański, C.S.Cierniewski The specificity and function of the reconstituted αIIbβ3 and αvβ3 minireceptors p 19. W.Okrój, B.Pietrzyk, L.Klimek, W.Jakubowski, W.Szymański, M.Kamińska, Przybyszewska-Doroś, P.Komorowski, B.Walkowiak Thrombocompatibility of titanium oxide deposited on stainless steel by sol-gel method p 20. I.Przybyszewska-Doroś, M.Kamińska, W.Okrój, W.Jakubowski, W.Szymański, P.Komorowski, B.Walkowiak Blood platelets activation by artificial surfaces

19:00 – Pub tour - pubs on Piotrkowska Street

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Wednesday, June 28th, 2006 Session VIII Chairmen: Zofia Pawlowska and Stan Heptinstall 9:00 – 9:55 Invited lecture VI level –1 Patricia B Maguire (Ireland) Using platelet proteomics to identify potential therapeutictargets 9:55 – 10:40 Invited lecture VII Maria Zellner (Austria) Biological variations and age-related changes in the platelet proteome 10:40 – 11:00 Coffee break Session IX Chairmen: Urszula Kralisz and Gianpiero Pescarmona 11:00 – 12:15 Oral presentations 18-22 level –1 18. GP.Pescarmona, E.De Vivo Platelets as a diagnostic tool for evaluation of hormonal status in ageing (15 minutes) 19. M.Reinhöfer, C.Macholdt, U.Franke, D.Barz, G.Marx, K.Reinhart, W.Lösche Monitoring peri-operative haemostasis by ROTEM thromboelastometry in patients with cardiac surgery (15 minutes) 20. J.Baranyai, J.Winning, R.A.Claus, I.Eisenhut, J.Hamacher, K.Reinhart, M.Bauer, W.Lösche

Inhibition of platelet function by aspirin or clopidogrel reduces organ injury in sepsis: first clinical and experimental data (15 minutes) 21. T.S.Charnavets, V.M.Mazhul The role of platelets phospholipids metabolism products in the mechanisms of protease-induced platelet aggregation (15 minutes) 22. W.Kuliczkowski, I.Pawlaczyk, M.Czuba, B.Karolko, R.Gancarz

The in vitro antiplatelet effect of the extract from Conyza canadensis - preliminary report (15 minutes) 12:15 – 12:30 Closing remarks level –1 12:30 – 14:00 Lunch

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Sunday, June 25th, 2006 9:00 – 17:00 Registration level 0 18:00 – 18:10 Welcome Message level –1 18:10 – 18:55 Opening lecture level –1 Gustav Born (U.K.) Current questions from earlier answers 19:00 – 20:30 Welcome Reception level –1