Lipoprotein Subfractions
Transcript of Lipoprotein Subfractions
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Lipoprotein Subfractions
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Douglas Wood, MT(ASCP) MCSEDivision of Chemistry and
ToxicologyOffice of in Vitro Diagnostics
Center for Devices and Radiological Health
Food and Drug Administration
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Lipoprotein Subfractions
The identification of lipid fractions
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Lipoprotein Subfractions
The identification of lipid fractions
The cholesterol pathway
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Lipoprotein Subfractions
The identification of lipid fractions
The cholesterol pathway
Effects of cholesterol
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Lipoprotein Subfractions
The identification of lipid fractions
The cholesterol pathway
Effects of cholesterol
Public Health concerns
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Lipoprotein Subfractions
The identification of lipid fractions
The cholesterol pathway
Effects of cholesterol
Public Health concerns
Lipid subfractions
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Lipoprotein Subfractions
The identification of lipid fractions
The cholesterol pathway
Effects of cholesterol
Public Health concerns
Lipid subfractions
Pertinent research
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Lipoprotein SubfractionsThe identification of lipid fractions
The cholesterol pathway
Effects of cholesterol
Public Health concerns
Lipid subfractions
Pertinent research
Subfraction recommendations
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Lipoprotein SubfractionsThe identification of lipid fractions
The cholesterol pathway
Effects of cholesterol
Public Health concerns
Lipid subfractions
Pertinent research
Subfraction recommendations
Conclusions
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Literature Review
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Literature Review
PUBNET and MedLine
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Literature Review
PUBNET and MedLine
Keywords – Lipoprotein, Lipoprotein Fractions, Lipoprotein Subfractions, LDL, HDL, Cholesterol and Electrophoresis
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Literature Review
PUBNET and MedLine
Keywords – Lipoprotein, Lipoprotein Fractions, Lipoprotein Subfractions, LDL, HDL, Cholesterol
Some articles selected were cited from other references that were found on PubNet and MedLine
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Literature Review
PUBNET and MedLine
Keywords – Lipoprotein, Lipoprotein Fractions, Lipoprotein Subfractions, LDL, HDL, Cholesterol
Some articles selected were cited in references found on PubNet and MedLine
A complete list of references used is available in the executive summary
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Types of Lipoproteins
LDL
B
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Types of Lipoproteins
E
B
C-ll
VLDL
B
LDL
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Types of Lipoproteins
E
B
C-ll
VLDL
B
LDL
B
IDL
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Types of Lipoproteins
E
B
C-ll
VLDL
B
LDL
B
IDL
A
HDL
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Cholesterol Metabolism
CholesterolBile Acids
GI Tract
Liver
Cholesterol
Oxidation
B
“Bad Cholesterol”LDL
CE
FC
CholesterolPool
A-I
HDL
CE
FC
“Good Cholesterol”
Oxidation
CholesterolPool
CholesterolBile Acids
GI Tract
A-I
HDL
CE
FC
“Good Cholesterol”
Cholesterol
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For decades, cardiovascular disease has been a major cause of death and
disability in developed countries –WHO ,World Health Report 2002
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For decades, cardiovascular disease has been a major cause of death and
disability in developed countries -WHO, World Health Report 2002
Despite significant reduction in mortality due to cardiovascular disease in recent years, it remains the leading cause of
death in the United States –AHA, 2006 Heart and Stroke Statistics
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Public Health InitiativesThe American Heart Association
Prevention Conferences
The National Cholesterol Education Program Adult Treatment Panel III
The National Academy of Clinical Biochemistry (draft)
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Public Health InitiativesThe American Heart Association
Prevention Conferences
The National Cholesterol Education Program Adult Treatment Panel III
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Public Health InitiativesThe American Heart Association
Prevention Conferences
The National Cholesterol Education Program Adult Treatment Panel III
The National Academy of Clinical Biochemistry (draft)
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Global Risk Factors
Framingham Risk Score
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Measurable Cardiac Risk Factors
Total Cholesterol
Low Density Lipoprotein Cholesterol (LDL-C)
High Density Lipoprotein Cholesterol (HDL-C)
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Measurable Cardiac Risk Factors
Total Cholesterol
Low Density Lipoprotein Cholesterol (LDL-C)
High Density Lipoprotein Cholesterol (HDL-C)
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Measurable Cardiac Risk Factors
Total Cholesterol
Low Density Lipoprotein Cholesterol (LDL-C)
High Density Lipoprotein Cholesterol (HDL-C)
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Candidate Biomarkers
C – reactive protein D-DimerFactors V, VII, VIII Lipoprotein (a) LDL Subfractions HDL SubfractionsHomocysteine MicroalbuminuriaApolipoprotein AI Apolipoprotein B Infectious Agents Apolipoprotein E
Subtype
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Candidate Biomarkers
LDL Subfractions
HDL Subfractions
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The Purpose of this panel meeting is to obtain input and
recommendations of the analytical and clinical validity of lipid
subfraction diagnostics assays
Purpose
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Austin, MA. et al, JAMA 1988
Lipid Profiles
Profile/Pattern A Profile/Pattern B
Lower risk for Greater risk forCardiovascular CardiovascularDisease Disease
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LIPOPROTEIN SUBFRACTIONSD
ensi
ty g
/mL
Diameter Å (Angstroms)
B
E
B
C-ll
EB
C-ll
EB
C-ll EB
C-ll
EB
C-ll
ChylomicronVLDL
B
B
B
LDL
HDL
AA
AA
A
2a
I
IIa
IVa
IIIa
IVbIIIb
IIb
2b3a
3b3c
Small Den
se
LDL
Large Buoyant
LDL
Large
HDL
Small
Dense
HDL
1.140
1.100
1.060
1.020
1.006
0.095
60 100 140 200 280 400 600 800 1000
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LIPOPROTEIN SUBFRACTIONS
Some investigators have identified significant differences in interpretation of the different
technologies used for lipid subfraction testing
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Simplified Terminology of Lipoprotein SubclassesSegmented Gradient Gel Electrophoresis
Pattern B Pattern Intermediate Pattern ALDL Particles IVb IVa IIIb IIIa IIb IIa I HDL Particles 3c 3b 3a 2a 2b
Nuclear Magnetic ResonancePattern B Pattern A
LDL Particles L1 L2 L3HDL Particles H1 H2 H3 H4 H5VLDL Particles V1 V2 V3 V4 V5 V6
Short Single Vertical Automated with Gradient UltracentrifugationPattern B Pattern A/B Pattern A
LDL Particles LDL4 LDL3 LDL2 LDL1HDL Particles HDL3(d,c,b,a) HDL2 (a,b,c)VLDL Particles VLDL 3b VLDL 3a VLDL 1 + 2
1. Bays HE, McGovern ME. Prev Cardiol 2003
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LIPOPROTEIN SUBFRACTIONS
EB
C-ll
VLDL
B
HDL LDL
AAAAA
2aIVb
2b3a3b3c
IIIaIIIaIIIb IIbIVa
Segmented Gel ElectrophoresisB BB BB
Nuclear Magnetic ResonanceA A A A A
A A A A A A
B B B
B
EB
C-ll
EB
C-ll
EB
C-ll
EB
C-ll
EB
C-ll
II
2 3 4 5
I 2
2
3
3
4 5 6
LDLHDL
AB B B B
EB
C-ll
EB
C-ll
EB
C-ll
EB
C-ll
Short Single Vertical with Gradient Ultracentrifugation
3c 3d 2a 2b 2c3b3a
HDL432I
LDLI 2 3a 3b
VLDL
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Ensign et al Findings
Gradient Gel Electrophoresis (GGE)
Density Gradient Ultracentrifugation
Nuclear Magnetic Resonance
Tube Gel Electrophoresis
1. Ensign W, et al Clinical Chemistry 2006
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Ensign et al Findings
Gradient Gel Electrophoresis
Density Gradient Ultracentrifugation (DGU)
Nuclear Magnetic Resonance
Tube Gel Electrophoresis
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Ensign et al Findings
Gradient Gel Electrophoresis
Density Gradient Ultracentrifugation
Nuclear Magnetic Resonance (NMR)
Tube Gel Electrophoresis
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Ensign et al Findings
Gradient Gel Electrophoresis
Density Gradient Ultracentrifugation
Nuclear Magnetic Resonance
Tube Gel Electrophoresis (TGE)
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Ensign et al Findings
N Nomenclature
Expected Values
Number of Subfractions
Substantial Heterogeneity
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Ensign et al Findings
N Nomenclature
Expected Values
Number of Subfractions
Substantial Heterogeneity
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Ensign et al Findings
N Nomenclature
Expected Values
Number of Subfractions
Substantial Heterogeneity
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Ensign et al Findings
N Nomenclature
Expected Values
Number of Subfractions
Substantial Heterogeneity
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Method 1 GGE
LDL is separated into 7 LDL subfractions based upon size and shape
Ensign et al Findings
B
I IIa IVaIIIa IVbIIIbIIb
B BB BBB
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Method 1 GGE
LDL is separated into 7 LDL subfractions based upon size and shape
LDL is separated into three patterns 26.35 – 28.5nm large LDL Pattern A
Ensign et al Findings
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Method 1 GGE
LDL is separated into 7 LDL subfractions based upon size and shape
LDL is separated into three patterns 26.35 – 28.5nm large LDL Pattern A
25.75 – 26.34nm Intermediate LDL Pattern AB
Ensign et al Findings
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Method 1 GGE
LDL is separated into 7 LDL subfractions based upon size and shape
LDL is separated into three patterns 26.35 – 28.5nm large LDL Pattern A
25.75 – 26.34nm Intermediate LDL Pattern AB22.0 – 25.74 small LDL Pattern B
Ensign et al Findings
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Method 1 GGE
LDL is separated into 7 LDL subfractions based upon size and shape
LDL is separated into three patterns 26.35 – 28.5nm large LDL Pattern A
25.75 – 26.34nm Intermediate LDL Pattern AB22.0 – 25.74 small LDL Pattern B
LDL subfractions are reported as percentages based on the area under the curve for each
subfraction
Ensign et al Findings
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Method 1 GGE
With this method small LDL particles correspond to LDL IIIa and LDL IIIb
Ensign et al Findings
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Method 1 GGE
With this method small LDL particles correspond to LDL IIIa and LDL IIIb
The findings of Ensign et al suggest that these subfractions, based upon GGE are indicators
of the severity of the atherogenic profile
Ensign et al Findings
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Method 2 DGU
Generates a series of absorbance curves which produces 6 LDL subclasses
Ensign et al Findings
B B B B B B
LDL 1 LDL 2 LDL 3 LDL 4 LDL 5 LDL 6
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Method 2 DGU
Generates a series of absorbance curves which produces 6 LDL subclasses
Subclasses are identified as Class I (most buoyant) through Class 6 (most dense)
Ensign et al Findings
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Method 2 DGU
Generates a series of absorbance curves which produces 6 LDL subclasses
Subclasses are identified as Class I (most buoyant) through Class 6 (most dense)
LDL 1 and LDL2 comprise pattern type A and LDL3 and LDL4 comprise pattern type B
Ensign et al Findings
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Method 3 NMR
Generates three LDL subclasses
Ensign et al Findings
BB B
L1 L2 L3
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Method 3 NMR
Generates three LDL subclasses
No references are provided for the basis of risk categories
Ensign et al Findings
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Method 4 TGE
LDL scan contains 7 possible LDL subfractions
LDL 1
Ensign et al Findings
B B B B B B B
LDL 2 LDL 3 LDL 4 LDL 5 LDL 6 LDL 7
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Method 4 TGE
LDL scan contains 7 possible LDL subfractions
Lipoproteins are separated to yield a score
Ensign et al Findings
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Method 4 TGE
LDL scan contains 7 possible LDL subfractions
Lipoproteins are separated to yield a score
Specific ranges of scores correspond to different LDL Patterns
Ensign et al Findings
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Method 4 TGE
LDL scan contains 7 possible LDL subfractions
Lipoproteins are separated to yield a score
Specific ranges of scores correspond to different LDL Patterns
Normal <5.5 (Pattern A)
Ensign et al Findings
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Method 4 TGE
LDL scan contains 7 possible LDL subfractions
Lipoproteins are separated to yield a score
Specific ranges of scores correspond to different LDL PatternsNormal <5.5 (Pattern A)
Intermediate 5.5 – 8.5 (Pattern AB)
Ensign et al Findings
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Method 4 TGE
LDL scan contains 7 possible LDL subfractions
Lipoproteins are separated to yield a score
Specific ranges of scores correspond to different LDL PatternsNormal <5.5 (Pattern A)
Intermediate 5.5 – 8.5 (Pattern AB)Atherogenic >8.5 (Pattern B)
Ensign et al Findings
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Method 4 TGE
Does not measure LDL particle size directly but estimates size by comparing electrophoretic mobility to the mobility of particles of known
sizes
Ensign et al Findings
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Ensign et al Findings
N Nomenclature
Expected Values
Number of Subfractions
Substantial Heterogeneity
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Ensign et al Findings
B
Gradient Gel ElectrophoresisB BB BBB
B B BBBB
Density Gradient Ultracentrifugation
Nuclear Magnetic ResonanceB BB
Tube Gel ElectrophoresisB B B B B B B
LDL 7
I IIa IIIa IVbIIIb IVaIIb
LDL 1 LDL 2 LDL 3 LDL 4 LDL 5 LDL 6
L1 L2 L3
LDL 1 LDL 2 LDL 3 LDL 4 LDL 5 LDL 6
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Ensign et al Clin Chem 2006
Distribution of LDL PhenotypesGGE DGU NMR TGE
A B AB0
510152025
30
35
Ensign et al Findings#
Peop
le
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Nomenclature
Expected Values
Total number of subfractions identified is method dependent
A substantial heterogeneity of interpretations with complete agreement in only 8% of
samples
Ensign et al Conclusions
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Established a link between LDL levels and CVD
NCEP ATP III Guidelines
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Established a link between LDL levels and CVD
Identify the combination of elevated triglycerides and Low HDL-C as an associated
risk of CVD
NCEP ATP III Guidelines
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Established a link between LDL levels and CVD
Identify the combination of elevated triglycerides and Low HDL-C as an associated risk of CVD
Recommend treatment of individuals at high risk based upon LDL-C and triglyceride values
NCEP ATP III Guidelines
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As LDL increases small dense LDL subfractions increase
Other Study Findings
Cromwell WC, Otvos JD. Current Atherosclerosis Reports 2004Campos H, et al. J Clin Endo Metab 1988;67:30-35.Asztalos BF, et al. Biochimica et Biophysics Acta 1993Asztalos BF, et al. Arterioscler Thromb Vasc Biol 2000Asztalos BF, et al. Aterioscler Thromb Vasc Biol 2003Asztalos BF, et al. Journal of Lipid Research. 2004Asztalos BF, et al. Aterioscler Thromb Vasc Biol 2004Asztalos BF, et al. Aterioscler Thromb Vasc Biol 2005Otvos JD, et al. Am J Cardiol 2002.Otvos JD, et al. Circulation 2006Otvos JD. et al. Clin Lab 2002
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As LDL increases small dense LDL subfractions increase
As HDL decreases there is a marked decrease in larger HDL particles
Other Study Findings
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As LDL increases small dense LDL subfractions increase
As HDL decreases there is a marked decrease in larger HDL particles
Based upon these findings, it has been suggested that elevated LDL, elevated small
dense LDL subfractions, low HDL and low HDL subfractions are predictive of CVD
Other Study Findings
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Although there is evidence that lipid subfraction profiles differ between individuals
with established CVD and normolipidemic individuals, it is unclear to the FDA whether
meaningful and reproducible diagnostic cutoffs for particle size, density and/or number
can be established
LIPOPROTEIN SUBFRACTIONS
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Some investigators have observed that lipid subfraction reference ranges for patient risk
for CVD (as defined by NCEP) vs normolipidemic patients have considerable
overlap
Other Study Findings
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HDL Large(mg/dL)
HDL Intermediate(mg/dL)
HDL Small(mg/dL)
HDL total(mg/dL)
Cholesterol total
(mg/dL)
Range 8-43 18 – 44 0 - 12 40 - 89 110 - 199
Mean 21.7 30.4 4.3 56.5 166.2
SD 8.05 5.06 2.56 10.87 19.37
96% range 10 - 41.9 22.0 - 41.9 1.0 - 11.0 41.0 - 79.9 118.5 - 197.8
HDL Large (mg/dL)
HDL Intermediate(mg/dL)
HDL Small(mg/dL)
HDL total(mg/dL)
Cholesterol total(mg/dL)
Range 2 - 90 13 – 53 1 - 19 21 - 122 94 - 322
Mean 14.5 28.1 6.2 49.0 213.9
SD 10.3 7.01 3.05 15.54 39.17
96% range 3.8 - 37.0 16.8 - 43.2 1.0 - 12.6 27.0 - 85.8 124.6 - 299.4
HDL Fractions comparisonNormolipidemic
Dyslipidemic
Morais et al, Poster presented at AACC 2005
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The NCEP ATPIII guidelines recognize that small LDL particles have been identified as a component of atherogenic dyslipidemia, and that some studies have suggested that some HDL subfractions may make important contributions to CVD risk assessment.
NCEP Guidelines
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The guidelines state that LDL particles are formed in large part as a response to elevated triglyceride. However, while these guidelines assert that LDL subfractions plus elevated triglyceride is associated with CVD, they also note that the ability of LDL subfractions to predict CVD independently of other risk factors is not well defined.
NCEP Guidelines
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NCEP Guidelines
The guideline also points out that the clinical performance of HDL subfractions has not been established. As a result of this and the ready availability of standard methodologies, the ATPIII does not recommend the measurement of small lipid particles in routine practice.
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NACB Draft Recommendations
In addition, the NACB recently proposed new guidelines for the use of several biomarkers for the assessment of CVD risk. The NACB is proposing the following three recommendations concerning lipid subclasses:
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NACB Draft Recommendations
1. Lipid subclasses, especially the number or concentration of small dense LDL particles have been shown to be related to the development of initial coronary heart disease events, but the data analysis of existing studies are generally not adequate to show added benefit over standard risk assessment.
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NACB Draft Recommendations
[Classification/Weight of Evidence: The committee found that there is evidence and/or general agreement that measurement of lipid subclasses is not useful (and in some cases might be harmful) based on data obtained from multiple randomized clinical trials that involved large numbers of patients.]
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NACB Draft Recommendations
2. There is insufficient data that measurementof lipid subclasses over time is useful to evaluate the effects of treatments.
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NACB Draft Recommendations
[Classification/Weight of Evidence: The committee found that there is conflicting evidence and/or divergence of opinion about the usefulness/efficacy of these assays, with the usefulness/efficacy of the test being less well established. This conclusion was based on a consensus of opinion of the experts in thefield.]
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NACB Draft Recommendations
3. Several methods are available to assess lipoprotein subclasses. Standardization is needed for this technology.
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NACB Draft Recommendations
[Classification/Weight of Evidence: The committee found that there is conflicting evidence and/or divergence of opinion about the usefulness/efficacy of standardization, with the weight of evidence/opinion being in favor of standardization. This conclusion was based on a consensus of opinion of the experts in the field.]
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The proposed recommendations cited above and the published reports provide insight regarding the current understanding of the clinical usefulness of these types of assays and the strengths and weaknesses of these potential biomarkers.
Conclusion
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However, FDA’s task when evaluating whether a novel assay should be cleared (or approved) is to determine whether the assay can be found substantially equivalent to existing assays (or is reasonably safe and effective for its intended use).
For that purpose we focus on the analytical and clinical validity of the assay based on the specific claim(s) that are made when promoting and labeling the device.
FDA Comments
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The FDA seeks the advice of this panel regarding whether the clinical use of these devices pose a risk to the public health.
FDA also requests that the panel discuss the effectiveness of these devices to measure and diagnose lipid disorders and atherosclerosis.
FDA Comments