Transfection of alternative cell lines RAW 264.7 3T3-L1 J774 AtT-20.
Lipidomic Profiling of RAW 264.7 Macrophages: The FXM Project
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Lipidomic Profiling of RAW 264.7 Macrophages: The FXM Project Jeffrey S. Forrester, Stephen B. Milne, Pavlina T. Ivanova, Michelle D. Armstrong and H. Alex Brown AfCS Lipidomics Lab, Department of Pharmacology & VICB, Vanderbilt University Medical Center. Introduction. - PowerPoint PPT Presentation
Transcript of Lipidomic Profiling of RAW 264.7 Macrophages: The FXM Project
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Lipidomic Profiling of RAW 264.7 Macrophages: The FXM Project
Jeffrey S. Forrester, Stephen B. Milne, Pavlina T. Ivanova, Michelle D. Armstrong and H. Alex Brown
AfCS Lipidomics Lab, Department of Pharmacology & VICB, Vanderbilt University Medical Center
Introduction:
The primary goal of the Alliance for Cellular Signaling (AfCS) is to determine how cells process signaling information in a context dependent manner. The AfCS has selected the RAW 264.7 cell line as the system for study, and the current modeling effort includes a broad based ligand screen and the FXM module. The FXM project is a focused effort by the AfCS to begin in-depth modeling of the signaling network originating from three pathways in the RAW 264.7 cell line. For this project, the AfCS is collecting a variety of response data after stimulation with uridine 5-diphosphate (UDP), complement C5a (C5a), or activation of the endogenous FcgR1 receptor. In addition, molecular interventions including RNAi and several drugs will facilitate the mechanistic description of the pathways originating from these stimuli.
The Lipidomics laboratory is taking part in the FXM project by creating multiplexed lipid arrays that highlight changes in the phospholipids in RAW 264.7 cells after stimulation. We have identified over 450 lipid species present in extracts from these cells. We have analyzed the response to UDP, C5a, and their combination; the corresponding lipid arrays are presented here. Stimulation with C5a induced a rapid response that included decreases in several species of phosphatidyl-ethanolamine (PE) and phosphatidylcholine (PC), with accompanying increases in phospholipase products. Stimulation with UDP increased several species of phosphatidic acid (PA), measurable 2 min. after stimulation and produced a concomitant decrease in several phosphatidylinositol (PI) lipids. The simultaneous addition of C5a and UDP produced an additive effect in response to these ligands over the majority of the arrays, with a few regions of possible interaction.
Lipid arrays were created using methods described in the AfCS research report, Milne et al., (2003) AfCS Res. Reports (www.signaling-gateway.org/reports). Each array represents a summary created from seven individual arrays. Data were collected at seven time points (0.25, 0.5, 2, 7, 15, 30, and 120 min.). each repeated in triplicate. Statistically significant changes in lipid species between basal and stimulated states are indicated with colors: red (relative increase), blue (relative decrease), and green (no change).
Introduction.
Figure 2. Mass Spectrometry Data Analysis.
Mass spectral data are statistically normalized and a Shewhart Control chart is constructed for the mean signal at each peak identified. This chart tests for time based stability in the basal condition and profiles the variability in the mean signal strength.
Table 1. Phospholipid Fragmentation
Library for RAW 264.7
Macrophages as Determined by CID MS/MS.
Figure 4. C5a Stimulation.
Lipid array constructed from seven sets of RAW 264.7 cells stimulated with 100 nM C5a.
Figure 6. UDP Stimulation.
Lipid array constructed from seven sets of RAW 264.7 cells stimulated with 1 mM UDP. (C*) Data showing measurement of intracellular free Ca2+ concentrations in RAW 264.7 cells after addition of 25 mM UDP.
Table 1. Results of RAW 264.7 Stimulation
Table 1. Results of RAW 264.7 Stimulation
Table 1. Results of RAW 264.7 Stimulation
Table 1. Results of RAW 264.7 Stimulation
The response to C5a is robust and complex. Observations include decreases in several species of PEs and PCs with concomitant increases in lysophospholipid species that are likely the result of a combination of PLA1 & PLA2 isoenzymes. It is interesting that the lipid response to C5a in RAW 264.7 cells is similar to that of WEHI 231 cells in response to IgE. The possible link between the secretion of signaling molecules in both WEHI 231 & RAW 264.7 cells is noted.
A preliminary substrate-product analysis of the UDP results suggests the sequential action of a PLC followed by a diacylglycerol kinase (DGK) in the production of the phosphatidic acid. In the next phase of these experiments perturbations such as small molecule inhibitors and specific siRNAs will be used to test proposed schemes. For example, cell preparations are being pretreated with pertussis toxin and LY294002, among others, to identify the constituents of these signaling cascades.
http://www.signaling-gateway.org
Discussion.
Figure 3. Mass Spectrometry Data Analysis.
The mean signal in the stimulated condition is compared with the profile generated in Step 2. Increasing or decreasing lipid species are highlighted and an array is created for the individual experiment. The entire process is then repeated and the resulting individual arrays are summed to increase statistical confidence in the result.
Figure 1. Lipidomic Profiling Overview.
Mass Spectrometry:
Mass spectral analysis was performed on a Finnigan TSQ Quantum triple quadrupole mass spectrometer (ThermoFinnigan, San Jose, CA) equipped with a Harvard Apparatus syringe pump and an electrospray source. Samples were analyzed at an infusion rate of 10 ml/min in both positive and negative modes over the range of m/z 400 to 1200. Data collected with the Xcalibur software package (ThermoFinnigan) were analyzed by a software program developed in our research group.
Figure 7. C5a and UDP Dual Ligand Stimulation.
Lipid array constructed from seven sets of RAW 264.7 cells stimulated simultaneously with 100 nM C5a and 25 mM UDP.
Figure 8. C5a and UDP Dual Ligand Stimulation.
Lipid array constructed from seven sets of RAW 264.7 cells stimulated simultaneously with 100 nM C5a and 25 mM UDP.
Figure 5. C5a Stimulation.
Lipid array constructed from seven sets of RAW 264.7 cells stimulated with 100 nM C5a.
C5a with Highlights
(Negative Mode Ionization, Seven Data sets)
Lipidm/z
0.25
0.5271530120
684.481110111
685.481110111
32:2PE686.480100011
687.46-2-2-3-2-3-3-1
32:1PE688.43-5-4-5-4-3-4-3
689.42-2-3-4-4-2-2-1
32:0PE690.41-5-4-5-5-6-6-4
691.36-1-1-10-10-2
Time (min.)
Lipidm/z
0.25
0.5271530120
713.440111111
34:2PE714.41-10-1-201-1
715.40-2-1-4-4-2-3-2
34:1PE716.41-2-2-4-30-1-2
717.40-3-3-4-4-2-3-3
34:0PE718.42-3-4-4-6-4-6-4
Time (min.)
Lipidm/z
0.25
0.5271530120
588.380112221
589.401356444
590.451212023
34:2DAG591.442403354
592.462323243
34:1DAG593.455544465
594.432436566
34:0DAG
18:2LPI
595.382322343
596.342444555
616.411112111
30:1PA
20:5LPI
36:3DAG
617.421415255
618.471322335
20:4LPI
30:0PA
36:2DAG
619.410213124
620.51-1100-121
20:3LPI
36:1DAG
621.47-1000-101
Time (min.)
Lipidm/z
0.25
0.5271530120
560.320000000
22:3LPG561.330113231
562.382546465
22:2LPG563.392264775
564.430220010
32:1DAG565.420121333
566.401121332
32:0DAG567.393355454
22:6LPS568.281021222
Time (min.)
Lipidm/z
0.25
0.5271530120
767.44000011-1
32:0PC(Cl)
38:3PE
768.44101010-2
36:4PG769.450-1-3-1-1-2-1
38:2PE770.46-4-2-5-5-3-6-4
36:3PG771.46-1-2-20-100
772.46-2-4-2-2-3-3-4
36:2PG773.450020111
Time (min.)
Lipidm/z
0.25
0.5271530120
Lipidm/z
0.25
0.5271530120
Lipidm/z
0.25
0.5271530120
Lipidm/z
0.25
0.5271530120
400.203444454629.421133232856.50010211-11069.890100000
401.215556666630.43113322336:4PI857.48-20000-1-11070.9000-1-20-1-1
402.235656556631.421133233858.4922312321071.300000000
403.241433233632.48225633536:3PI859.48-2-11-10101071.8800-1-1100
404.201434333633.461335124860.5134323421072.271111011
405.192333342634.51011011236:2PI861.5013403321072.850-1001-2-2
406.204553545635.490111111862.5101303421073.400000000
16:1LPA407.180201130636.46112123336:1PI863.5101101111073.820-1-100-1-1
408.190101111637.426465565864.52-1-1-100001074.170000000
16:0LPA409.20-1000000638.424456665865.52-1-20-1-1-111074.801-2-2-2-1-1-2
410.21-1001222639.422345445866.53-1001-2-201075.70010-1-10-1
411.201010221640.433445445867.5200001-2-11075.86-10-1-1-100
412.264443454641.433234423868.560-1-100-2-21076.272221211
413.214543455642.440000000869.541-1-1-1-1-2-21076.761011210
414.231231334643.4100-11000870.570-1-2-1-2-201076.940000000
415.203443333644.460000010871.5310100001077.89-1-10-2-2-1-2
416.212444555
32:1PA
22:5LPI
645.42-1000-1-10
40:4PC(C
l)
872.5621-101-2-11078.25-1-1-1-1-1-1-1
417.250110121646.510010-121873.55-1-2-100001078.79010-1-1-1-1
418.241011221
32:0PA
22:4LPI
38:2DAG
647.50-1110-110874.56100020-11079.151221122
419.233222442648.54-12-1-1-112875.56-3-10-11021079.830-1000-1-1
420.23222332222:3LPI649.54-1000-101876.5810201-101080.190000000
421.210110321650.52-10-1-1-1-12877.58222133-11080.70-110-2000
14:1LPE422.22121133-122:2LPI651.482132212878.5711111001080.9700-1-1000
423.23-1000211652.440112012879.5611001-1-21081.6500-1-1-100
14:0LPE424.230202222653.401123211880.54111011-11081.830-10000-1
425.230012241654.461223220881.500-1-2-1-1-201082.240000000
426.244534444655.411212111882.5200010-1-11082.7900000-1-1
427.222332434656.47100110138:5PI883.49-1-2-1000-11083.192112221
428.241221344657.451012102884.50-10-1011-11083.8400000-1-1
429.12-1100000658.46100211238:4PI885.5202111211084.88-2-2-2-30-10
430.14-1000-100659.450001111886.51-12101101085.87-10-1-2-200
431.191212243660.461111-11238:3PI887.5313101211086.800000000
432.220212443661.44-10000-11888.54-110-120-11086.920000000
433.20001232130:0PE662.48-1010-10138:2PI889.55-10-100001087.87-2-2-3-2-1-1-1
434.201000110663.452444123890.5601101211088.81-100-20-20
18:1LPA435.210011011664.493343222891.5700111111088.91-10-1-1000
15:1LPE436.230000010665.452212322892.5610111101089.630-101-100
18:0LPA437.26-1210000666.000000000893.57232221-11089.9300-10-10-1
15:0LPE438.26-1210000666.472234214894.6023112001090.74-1-200-2-1-1
439.242422222666.960000100895.6010000001091.16-100-1-1-10
440.253342442667.422323332896.6300-10-1-101091.78-1-2-1-2-10-1
441.232332441668.451122122897.59-100-1-1-101092.23-10-1-1-2-1-1
442.251133443669.421222112898.580-2-2-1-1-101092.90000-1-1-10
443.242222554670.461101111899.56-2-1000001093.180-10-2-1-1-1
444.26111322334:2PA671.39-3-1-200-1-2900.60-2-1-1-11001093.81-1-1-10000
445.250211111672.41-1-2-2-1-200901.580-1100001094.170000000
446.27000022134:1PA673.36-2-2-2-3-1-2-2902.6311011-111094.730000000
447.242112421674.41-3-3-1-1-2-10903.6321111001095.101111111
448.24001011134:0PA675.42-1-110-2-11904.7110000011095.86-1-2-1-1-2-1-1
449.24011222232:0PEe676.460000-1-11905.6811111121096.19-1-10-1-1-1-1
16:1LPE450.24-1-1-1-1110677.4400020-10906.67000-10001096.92100-100-1
451.241221210678.431000000907.5800110001097.940-2-1-20-3-1
16:0LPE452.25-1-1-1-1000679.392223211908.5900-1-10-1-11098.91-1-1-1-10-1-2
453.261212102680.451112121909.54-10-1100-11099.86-2-2-1-2-2-3-2
454.273332332681.431112211910.53-1-1-1100-11100.101110111
14:0LPG455.240023133682.470100110911.5400-100101100.86-1-10-1-2-11
456.243454555683.471111110912.55-2-1-1-1-10-11101.2900-1-1-1-1-1
457.240011220684.481110111913.55-20-1-10-1-21101.850-10-1000
458.251011110685.481110111914.57-10-10-10-11102.340000000
459.28-220000032:2PE686.480100011915.5701000001102.811-101110
460.29-2200000687.46-2-2-3-2-3-3-1916.5801000001103.140-1010-10
461.28044133332:1PE688.43-5-4-5-4-3-4-3917.5801110101103.770-10-1-100
462.28-12-1-1220689.42-2-3-4-4-2-2-1918.6200010011104.07-2-3-2-2-2-1-2
463.26121121132:0PE690.41-5-4-5-5-6-6-4919.6200000001104.710-100101
17:1LPE464.260111102691.36-1-1-10-10-2920.6400000001105.07-2-2-1-1-3-2-3
465.260022442692.380001121921.6410000001105.340000000
17:0LPE466.270121220693.360001102922.6410001101105.860-1-10002
467.261111331694.390002222923.65100-10101106.242222222
468.270111210695.400210101924.6410000001106.83-1-20-2-1-10
469.240211110696.440112102925.6310-1-10-101107.19-1-1-10-1-1-1
470.25-100101136:3PA697.40-1-1-2-2-2-10926.6110-10-1-101107.76001-1-1-10
471.233323432698.43-110-10-1-1927.59-10-101-111108.150-10-1-1-10
472.27120232136:2PA699.39-10-2-21-1-1928.580-1-1-10-1-11108.82-1000-100
473.260111001700.400-1-3-3-1-3-1929.60-1-1-1-1-1-1-11109.310000000
18:3LPE474.27-100001036:1PA701.40-2-1-2-2-1-1-1930.7010000001109.87-2-2-5-2-20-2
475.27343265333:1PE702.41-3-3-3-4-3-3-2931.6700100001110.420000000
18:2LPE476.24-1-1-1-1000703.41-3-5-3-3-2-4-2932.72000000-11110.90-1-2-2-20-21
477.25101244433:0PE704.42-3-6-4-5-3-4-2933.69000-1-1-1-11111.321101001
18:1LPE478.24-1000-100705.411-2-10-2-20934.7100000001111.790000101
479.250000000706.412112110935.670-1-100001111.92-1-10-2-2-1-2
18:0LPE480.26-3-3-2-2-1-1-1707.381112110936.670-1-200001112.310000000
16:1LPG481.240000100708.440011010937.6401-10-1001112.89-2-3-1-3-1-1-1
482.250000000709.412233222938.661-1-11-1-201113.880-10-10-1-1
16:0LPG
26:0DAG
483.240000000710.480012-1-13939.631300-1-101114.40-100-1-1-1-1
484.250010111711.452244233940.6311-1-10-1-11114.920-1-10-10-1
485.22000001-1
28:0PC(Cl)
34:3PE
712.472043123941.620-1-1-1-1-2-11115.340000000
486.240000000713.440111111942.61000-10-1-11115.93-2-4-3-2-1-2-2
487.22021010134:2PE714.41-10-1-201-1943.600-10-100-11116.860000001
488.35-1101110715.40-2-1-4-4-2-3-2944.6310101101117.07-1-1-2-1-1-1-2
489.29102221034:1PE716.41-2-2-4-30-1-2945.632-1-1-10001117.601011111
490.291011100717.40-3-3-4-4-2-3-3946.6600001001118.021111111
491.27022231134:0PE718.42-3-4-4-6-4-6-4947.1300000001118.36-3-2-3-3-3-3-3
19:1LPE492.27-1-1-1-2-1-1-132:1PG719.400000000947.6400100001118.9400000-10
493.270000101720.390000-100948.690-2-1-10001119.780000000
16:1LPS
19:0LPE
494.260000000
32:0PG
721.39-1-10-1-1-10949.6610001101120.011-101100
495.26022101136:4PEp722.41000-100-1950.700-10-11-101120.7400-10000
16:0LPS496.281110100723.40-1-1-2-1-1-1-1951.5800000011121.021111110
497.273232011724.4200-1-2-10-1952.6000000001121.73-1-1-1-1-1-1-1
20:5LPE498.241210010725.40-1-2-1-2-1-2-1953.59001-10-101121.971101-111
499.240101110726.420-1-1-2-1-10954.59001-10001122.371111111
20:4LPE
14:1LPC(Cl)
500.230101001727.41-100-2-1-10955.6201010011122.83-1-1-1000-1
501.24-1-100111728.43-1-1-2-3-1-3-1956.661-1000-1-11123.00-1-1-1-2-2-2-2
20:3LPE
14:0LPC(Cl)
502.250000000729.43-1-1-2-2-1-20957.7000001001123.400000000
503.25000111135:1PE730.44-4-3-4-6-3-4-4958.71200-10001123.9400-10-100
20:2LPE504.29-1-1-1-1000731.43-3-3-3-4-4-3-2959.7201100111124.41100-1000
505.28000000032:1PS732.41-2-2-2-3-1-1-2960.730-1-2-2-1-1-11124.99010010-1
20:1LPE506.29-2-3-2-3-1-1-1733.40-2-3-1-1-2-1-1961.7011101-101125.8600000-11
18:2LPG507.28-1-1-1-10-1-132:0PS734.430-1-100-11962.7010001001126.210000000
20:0LPE508.3400-1-20-11735.421000100963.68-1-1-1-10-101126.80-1-1000-10
18:1LPG
509.260000000
30:2PC(Cl)
36:5PE
736.420-10-10-1-1964.7200011-101126.9600-10-100
510.270000000737.430000110965.68-100110-11127.910-1-2-1-2-2-2
18:0LPG
28:0DAG
511.290000210
36:4PE
30:1PC(Cl)
738.430000110966.71-1-10-1-10-11128.92-1-100-100
512.280100001739.430000110967.6300100001129.361211111
513.260010221
36:3PE
30:0PC(Cl)
740.42101011-1968.6300000001129.92-1-100000
15:1LPC(Cl)
514.27-10-1-1011741.42-10-2-1000969.65-1-1-1-2-1101130.221111111
515.28-101000036:2PE742.43000-11-1-1970.660-1-1-1-1001130.8500-100-1-1
15:0LPC(Cl)
516.310000000743.43-1-3-4-50-3-2971.66-100-1-1-1-11131.29000-1-10-1
517.30000000036:1PE744.44-1-2-3-3-1-2-3972.6700001001131.970000000
518.420-10000134:2PG745.430-110000973.6600001-101132.540000000
519.40010001038:6PEp746.420010111974.68-1000-1001132.93-2-2-3-2-1-2-1
18:2LPS520.35010011134:1PG747.420030122975.6500001001133.370000000
521.32011201038:5PEp748.420-10011-1976.68100100-11133.91-1011-100
18:1LPS522.30000001134:0PG749.420-100-10-1977.6501111101134.291110111
523.310110321750.44000-10-1-1978.69000-10-201134.94-20-100-1-1
18:0LPS
22:6LPE
524.250000000751.4300-1-3-1-20979.6801001-1-11135.350000000
525.26000000031:1PC752.45001-10-1-2980.7010001001135.91-2-2-1-1-10-1
22:5LPE526.250000000753.450-1-1-1-1-10981.6801100001136.940-1-1100-1
527.26-1-1-1-1000
31:0PC(Cl)
38:3PEe
754.450-1-3-2-1-3-1982.6900-101001137.390000000
22:4LPE
16:1LPC(Cl)
528.260000000755.45-1-3-2-4-1-3-1983.691111-1-1-11137.96-2-4-2-2-1-2-1
529.26-1000000756.46-2-3-3-4-2-4-2984.730-1-1-1-1-2-11138.75-1-1-1-1-102
22:3LPE
16:0LPC(Cl)
530.260000010757.45-4-3-2-4-2-4-2985.74-100-1-1101138.940000010
20:4LPG531.27000000034:2PS758.45-1-1-1-30-1-1986.7400001011139.320-1-1-1-10-1
22:2LPE532.280000000759.44-1-2-2-1-4-2-1987.7110001-111139.80000-10-10
20:3LPG533.28000000034:1PS760.430-110110988.7401011111140.04-1-2-2001-1
22:1LPE534.36-1-10-2000761.430-110-100989.73-10100101140.90-1-1-3-2-2-1-3
20:2LPG
535.34-110-1222
34:0PS
38:6PE
762.44-1-1-10100990.7101011001141.350000000
C16:0S536.440110021763.44-1-10-1110991.6902122201141.90-1-1-2-1-1-2-1
20:1LPG
537.440100111
38:5PE
32:2PC(Cl)
764.4411-1-111-1992.7301111201142.7600-10-1-10
538.43-212-1011765.450100010993.7200000-1-11143.000-10-10-1-1
30:0DAG
539.410000122
32:1PC(Cl)
38:4PE
766.43101120-2994.71-1-10-1-1-1-11143.421101111
540.37010-1344767.44000011-1995.7100000001143.850-2-1-1-2-10
541.301032331
32:0PC(Cl)
38:3PE
768.44101010-2996.7011112101144.070-1-1-1-2-10
542.30122323436:4PG769.450-1-3-1-1-2-1997.7200011-101144.861-1-2000-1
543.291-11122138:2PE770.46-4-2-5-5-3-6-4998.7300000001145.203233112
20:4LPS
17:0LPC(Cl)
544.341223222
36:3PG
771.46-1-2-20-100999.700-1-10-1-1-11145.87-2-2-1-1000
545.350222322772.46-2-4-2-2-3-3-41000.7100-100001146.281111111
20:3LPS546.34010000136:2PG773.4500201111001.70-10-100001146.72-2-3-3-1-2-1-1
547.33011133340:6PEp774.4600201111002.701-1-100001146.980000000
20:2LPS548.32000001136:1PG775.4600101111003.6810-10-1-1-11147.60-1-1-1-10-1-1
549.311131421776.47-10001011004.2000000001147.90-3-3-2-3-1-10
20:1LPS550.411021322777.4834411541004.71-10000001148.34-1-1-1-10-1-1
551.382213553
33:2PC(Cl)
778.4734233331005.6900000001148.920-2-2-10-2-1
20:0LPS
18:3LPC(Cl)
552.330000100779.4820112221006.720-1001001149.361111111
553.341110322
33:1PC(Cl)
780.4600-1-10101007.700-1000001149.9100-1-10-10
18:2LPC(Cl)
554.362434443781.4720110-1-11008.2000000001150.42-2-100-1-1-1
22:6LPG
555.332355455
33:0PC(Cl)
36:4PS
782.490-1-2-2-1-2-11008.7100001001150.98-1-11-1201
18:1LPC(Cl)
556.292221222783.49-1-4-2-4-4-3-21009.7401101111151.652210222
22:5LPG557.291022222784.480-3-2-2-1-2-11010.770000-1-2-21152.040000010
18:0LPC(Cl)
558.350000011785.48-1-1-2-4-2-3-11011.77-10000111152.46-1-1-1-1-1-1-1
22:4LPG559.35011001136:2PS786.4601111211012.79-1-10-1-1-1-11152.98-2-2-1-2-1-10
560.320000000787.460010-1001013.7900000-1-11153.8900-1-1-100
22:3LPG561.33011323136:1PS788.461-1-1-10211014.81000-10-201154.590000000
562.382546465789.471-1000111015.780-10-1-1-1-11154.950-1-2-20-2-1
22:2LPG
563.392264775
36:0PS
40:6PE
790.47-10001101016.7701101-101155.710000000
564.430220010791.460-2-1101-11017.76-3-2-2-2-1-2-21155.93-1-1-1-1000
32:1DAG
565.420121333
34:2PC(Cl)
792.45100010-11018.7310000001156.750000000
566.401121332793.470-1-101-1-11019.2300000001156.960000000
32:0DAG
567.393355454
34:1PC(Cl)
794.48-1-1-1-11-101019.72111000-11157.420000000
22:6LPS568.281021222795.48111111-11020.7310000001157.90-1-20-2-100
16:1LPI
569.281112122
34:0PC(Cl)
796.48111111-11021.7100000-101158.491011101
22:5LPS
19:1LPC(Cl)
570.28-10-10001
38:4PG
797.490-10-10-2-11022.7210-1-10-1-21158.940-10-2000
16:0LPI571.280020002798.48-1-1-1-1-1-1-11023.71000-10001159.430000000
22:4LPS
19:0LPC(Cl)
572.422013113
38:3PG
799.4900-10-1-101024.730-11020-11159.84000000-1
573.412012112800.49-2-1-11-1-101025.730-1000-101160.140-100111
22:3LPS574.41201311338:2PG801.4900001011026.760-3-20-1-1-21160.840001022
575.411133123802.49-1-1-100-111027.7600-1-1-1-1-21161.010000000
22:2LPS576.410111122803.50-1-1010001028.79-1-1-2-2-1-3-21161.86-1-2-3-2-2-10
577.384456535804.4942212231029.760-1-1-2-1-2-21161.990-10000-1
22:1LPS
20:4LPC(Cl)
578.393456565805.4833112311030.78-1-2-3-3-2-3-31162.970000-1-1-1
579.383332665
35:2PC(Cl)
38:6PS
806.4722323331031.1700000001163.92-1-2-1-10-10
20:3LPC(Cl)
580.351210020807.4733334431031.730-1001-201164.9500-10000
581.361232333
35:1PC(Cl)
38:5PS
808.52311011-11032.73-1-1-1-1-1-201165.370000000
20:2LPC(Cl)
582.310010120809.5122421521033.2200000001165.94-1-1-1-100-1
583.32141444338:4PS810.5211201101033.77-1-2-1-2-1-201166.420000000
20:1LPC(Cl)
584.36000-2-100811.52-10100101034.75-10-2-2-1-111166.88000-1-10-1
585.35110011038:3PS812.4800000001035.76-10-1-1-1-101167.0500-1-10-1-1
586.42000-1010813.4900100101036.7900000001167.830011111
587.41212313138:2PS814.490-1000001037.80-1-1010001168.24-1-10-1-10-1
588.380112221815.481-1-1-1-1001038.82-1-1-1-100-11168.810000000
589.401356444
38:1PS
36:4PC(CL
)
816.500-2-101-2-11039.81-1-1-10-4-301168.9700-1-10-10
590.451212023817.481-1121121040.840-1-1-10-111169.91-10-1-1-2-1-1
34:2DAG
591.442403354
36:3PC(Cl)
818.51-1-2-2-20-2-11041.83-1-1-1-2-1-201170.440000000
592.462323243819.4812221111042.85000-1-1-1-11170.96-1-1-20-2-1-1
34:1DAG
593.455544465
36:2PC(Cl)
820.49110110-11043.84011-100-11171.460000000
594.43243656640:6PG821.490-1-1010-11044.8100001001171.94-1-11-1-1-1-1
34:0DAG
18:2LPI
595.382322343
36:1PC(Cl)
822.49110110-11045.7500000001172.571111011
596.34244455540:5PG823.50000011-11046.77-2-2-1-1-1001172.970000000
18:1LPI597.271000000824.50-10011-101047.74-1-1-1-1-2-1-11173.90-10-1-1-10-1
598.34011111040:4PG825.510-1010-1-11048.2100000001174.89-10-10000
18:0LPI599.320011001826.5000-10-2-2-11048.760-1-1-10-1-21175.900000000
600.402222222827.51-10-10-2-2-11049.1900000001176.910000000
601.401122320828.510-2-200-101049.78000-10-101177.92-10-10000
22:6LPC(Cl)
602.401122122829.5100-11-1-201050.8000000001178.9300000-10
603.392234433830.5032223121051.7600000001179.93-101-1-11-1
22:5LPC(Cl)
604.401123223831.4911011001052.73-2-2-1-1-1-101180.96-1-2-1-1-1-1-2
605.39333532340:7PS832.5033334431052.890-1-10-1-101181.96-2-1-3-2-1-2-2
606.46113332334:2PI833.4733303421053.3411111111182.970000000
607.430222324
40:6PS
37:2PC(Cl)
834.4824333421053.78-1-2-1-1-20-21183.400000000
22:3LPC(Cl)
608.400120121
34:1PI
835.4722312431054.7800-1-100-21183.9900-10000
609.38443566540:5PS836.480-1112321055.79-1-2-2-11-1-11184.450000000
610.36253463434:0PI837.490000-1001056.810-1-1-11001184.9810-110-11
611.38545566540:4PS838.51-11112111057.81-1-1-1000-11185.551222221
612.383424444839.500-110-1001058.82-10-1-1-1-1-11186.0000-11-100
613.392344554
38:6PC(Cl)
40:3PS
840.510-1-101-111059.2300000001186.471111111
614.383145332841.51-11122111059.8000110011186.991111101
615.402234333
38:5PC(Cl)
842.5210011-101060.2100000001187.171110111
616.411112111843.520-1-11-1-2-11060.81-1-1-2-21-1-21187.9100-10000
30:1PA
20:5LPI
36:3DAG
617.421415255
38:4PC(Cl)
40:1PS
844.560-1-2-11-101061.71-1-1-1-1-1-101188.100000000
618.471322335845.550-1-3-1-1-2-11061.8600000011188.552212022
20:4LPI
30:0PA
36:2DAG
619.410213124
38:3PC(Cl)
846.570-1-1010-21062.3500000001188.970000001
620.51-1100-121847.560-10-110-11062.82001-10011189.390000000
20:3LPI
36:1DAG
621.47-1000-101848.570-10010-11063.8600000001189.93-100-1-1-10
622.471-111001849.5710111101064.910-1000001190.9400-10-10-1
20:2LPI623.421123324850.570-10010-11065.860-1-100001191.91-1000000
624.413234435851.5700-1-110-11066.2200000001192.91-1000000
20:1LPI625.393454666852.5410-2-10-1-21066.870-1-100-1-11193.93-1000001
626.451123223853.520-1-1-11-3-11067.85-2-2-3-2-1-2-11194.95000-1-100
627.412234342854.51102121-11068.88111-2-1-301195.381111111
628.442223222855.51-1-10-101-11069.3900000001195.980000-100
1
2
3
4
5
6
1
2
3
4
5
6
negativepositivenegativepositivenegativepositivenegativepositive
40716:1 LPA 54820:2 LPS20:2 LPC68632:2 PE 78233:0 PC (Cl), 36:4 PS36:4PC
40916:0 LPA 55020:1 LPS20:1 LPC68832:1 PE
32:2 PE
78436:3 PC
42214:1 LPE 55220:0 LPS, 18:3 LPC (Cl)20:0 LPC, 24:6 LPE69032:0 PE 32:1 PE78636:2 PS36:2PC, 39:2 PE
42414:0 LPE14:1 LPE55418:2 LPC (Cl)24:5 LPE69232:0 PE78836:1 PS36:1PC, 39:1 PE
42614:0 LPE55522:6 LPG 69536:4 PA 79036:0 PS, 40:6 PE 36:1 PS
43518:1 LPA 55618:1 LPC (Cl) 69736:3 PA 79234:2 PC (Cl)
40:6 PE, 36:0 PS
43615:1 LPE 55722:5 LPG 69936:2 PA 79434:1 PC ( Cl ) 40:5 PE
43718:0 LPA 55818:0 LPC (Cl)24:3 LPE70030:3 PC79634:0 PC (Cl) 40:4 PE
43815:0 LPE 55922:4 LPG 70136:1 PA16:1 SM79738:4 PG
44012:0 LPC56122:3 LPG 70233:1 PE30:2 PC79840:3 PE
45016:1 LPE 562 24:1 LPE70316:0 SM79938:3 PG
45216:0 LPE16:1 LPE56322:2 LPG 70433:0 PE30:1 PC, 33:1 PE800
37:2 PC, 40:2 PE
45416:0 LPE564C18:0 S 70630:0 PC80138:2 PG
45514:0 LPG 56532:1 DAG 71228:0 PC (Cl), 34:3 PE 80240:1 PE
46417:1 LPE 56732:0 DAG 71434:2 PE34:3 PE80635:2 PC (Cl), 38:6 PS38:6 PC
46617:0 LPE14:1 LPC, 17:1 LPE56822:6 LPS22:6 LPC71634:1 PE34:2 PE807
46814:0 LPC, 17:0 LPE56916:1 LPI 71834:0 PE
34:1PE, 31:1 PC
808
35:1 PC (Cl), 38:5 PS
38:5PC, 38:6 PS
47418:3 LPE 57022:5 LPS, 19:1 LPC (Cl)22:5 LPC, 22:6 LPS71932:1 PG 81038:4 PS38:4 PC
47618:2 LPE18:3 LPE57116:0 LPI 720
31:0 PC, 34:0 PE
812 38:3 PS
38:3 PC, 38:4 PS
47818:1 LPE18:2 LPE57222:4 LPS, 19:0 LPC (Cl)22:4 LPC, 22:5 LPS72132:0 PG 81438:2 PS
38:2 PC, 38:3 PS
48018:0 LPE18:1 LPE, 15:1 LPC57422:3 LPS22:3 LPC72236:4 PEp 81638:1 PS, 36:4 PC (Cl)38:1 PC,
48116:1 LPG 57622:2 LPS22:2 LPC72338:4 PA 81836:3 PC (Cl)38:1 PS
48218:0 LPE, 15:0 LPC57822:1 LPS, 20:4 LPC (Cl)22:1 LPC72436:4 PEp82036:2 PC (Cl)
48316:0 LPG, 26:0 DAG 58020:3 LPC (Cl)22:0 LPC72538:3 PA 82140:6 PG
48813:0 LPC 58220:2 LPC (Cl) 72738:2 PA 82236:1 PC (Cl)42:5 PE
49219:1 LPE 58420:1 LPC (Cl) 72832:3 PC82340:5 PG
49416:1 LPS, 19:0 LPE16:1 LPC, 19:1 LPE59134:2 DAG 73035:1 PE32:2 PC82540:4 PG
49616:0 LPS16:0 LPC592C20:0 S 73232:1 PS32:1PC83042:1 PE
49820:5 LPE 59334:1 DAG, 18:3 LPI 73432:0 PS32:0PC83240:7 PS
50020:4 LPE, 14:1 LPC (Cl) 59534:0 DAG, 18:2 LPI 73630:2 PC (Cl), 36:5 PE32:0 PS83334:2 PI
50220:3 LPE, 14:0 LPC (Cl)20:4 LPE59718:1 LPI 73836:4 PE, 30:1 PC (Cl)36:5 PE83440:6 PS, 37:2 PC (Cl)40:6 PC
50420:2 LPE20:3 LPE59918:0 LPI 74036:3 PE, 30:0 PC (Cl) 36:4 PE83534:1 PI
50620:1 LPE20:2 LPE60222:6 LPC (Cl) 74236:2 PE 36:3 PE83640:5 PS40:5 PC, 40:6 PS
50718:2 LPG 60422:5 LPC (Cl) 74436:1 PE
33:2 PC, 36:2 PE
83734:0 PI
50820:0 LPE20:1 LPE60822:3 LPC (Cl) 74534:2 PG 83840:4 PS40:4 PC, 40:5 PS
50918:1 LPG 61730:1 PA, 20:5 LPI, 36:3 DAG 74638:6 PEp36:1PE, 33:1 PC84038:6 PC (Cl), 40:3 PS40:3 PC, 40:4 PS
51017:0 LPC, 20:0 LPE618C22:1 S 74734:1 PG 84238:5 PC (Cl)40:2 PC
51118:0 LPG, 28:0 DAG 61920:4 LPI, 30:0 PA, 36:2 DAG 74838:5 PEp38:6 PEp, 33:0 PC84438:4 PC (Cl), 40:1 PS40:1 PC, 40:2 PS
51415:1 LPC (Cl) 620C22:0 S 74934:0 PG 84638:3 PC (Cl)40:0 PC
51615:0 LPC (Cl) 62120:3 LPI, 36:1 DAG 75034:6 PC, 38:5 PEp 85736:4 PI
51818:3 LPC622 24:0 PC752
31:1 PC (Cl)
34:5PC85936:3 PI
52018:2 LPS18:2 LPC62320:2 LPI 75431:0 PC (Cl), 38:3 PEe34:4PC86136:2 PI
52218:1 LPS18:1 LPC62520:1 LPI 75634:3PC86336:1 PI
52418:0 LPS, 22:6 LPE18:0 LPC, 18:1 LPS64532:1 PA, 22:5 LPI 75834:2 PS34:2PC86442:5 PC
52622:5 LPE18:0 LPS, 22:6 LPE646C24:1 S 76034:1 PS34:1PC86642:4 PC, 42:5 PS
52822:4 LPE, 16:1 LPC (Cl)22:5 LPE64732:0 PA, 22:4 LPI, 38:2 DAG 76234:0 PS, 38:6 PE
34:0 PC, 34:1 PS
86842:3 PC
53022:3 LPE, 16:0 LPC (Cl)22:4 LPE648C24:0 S 76438:5 PE , 32:2 PC (Cl)38:6 PE, 34:0 PS87042:2 PC
53120:4 LPG 64922:3 LPI 76632:1 PC ( Cl ), 38:4 PE38:5PE87240:4 PC (Cl)42:1 PC
53222:2 LPE22:3 LPE650 26:0 PC76832:0 PC (Cl), 38:3 PE38:4PE88338:5 PI
53320:3 LPG 65122:2 LPI 76936:4 PG 884
53422:1 LPE22:2 LPE66030:1 PE 77038:2 PE38:3 PE88538:4 PI
53520:2 LPG 66230:0 PE 77136:3 PG 88738:3 PI
536C16:0 S22:1 LPE, 19:1 LPC664
30:0 PE
77235:2, PC88938:2 PI
53720:1 LPG 67134:2 PA 77336:2 PG 90940:6 PI
53819:0 LPC67334:1 PA 77440:6 PEp35:1 PC91140:5 PI
53930:0 DAG 674
C26:1 S
28:2 PC77536:1 PG 91340:4 PI
54220:5 LPC67534:0 PA 77640:6 PEp91540:3 PI
54420:4 LPS, 17:0 LPC (Cl)20:4 LPC67632:0 PEe, C26:0 S28:1 PC77833:2 PC (Cl)36:6 PC91740:2 PI
54620:3 LPS20:3 LPC678 28:0 PC 78033:1 PC (Cl)36:5PC91940:1 PI
m/zIonization Modem/zIonization Modem/zIonization Mode
m/z
Ionization Mode
C5a with Highlights
(Positive Mode Ionization, Seven Data sets)
Lipidm/z
0.250.5
271530120
809.58100-1-2-1-1
38:4PC810.570-2-2-1-3-3-3
811.590-1-20-3-4-4
38:3PC
38:4PS812.59-2-4-3-3-4-4-4
813.64-1-2-4-3-3-4-3
38:2PC
38:3PS814.63-2-3-3-2-3-4-2
815.66-1-3-2-3-3-40
38:1PC816.65-1-2-1-1-3-31
817.630-3-3-3-2-3-1
Time (min.)
Lipidm/z
0.25
0.5271530120
783.551-10-2-1-10
36:3PC784.550-4-3-2-2-4-4
785.57-1-1-3-1-1-3-2
36:2PC
39:2PE786.57-2-4-3-4-5-4-4
787.59-1-3-3-4-5-5-3
36:1PC
39:1PE788.58-2-5-4-5-5-5-5
789.59-1-3-4-1-3-4-3
36:1PS790.59-1-2-3-2-2-30
791.591-100-100
Time (min.)
Lipidm/z
0.25
0.5271530120
755.501000000
34:3PC756.511-1-2-1-1-1-1
757.5200-1-1-10-1
34:2PC758.53-1-1-3-4-5-4-4
759.55-2-2-2-2-3-3-2
34:1PC760.55-2-3-3-5-4-4-4
761.55-1-3-3-4-4-5-4
34:0PC
34:1PS762.56-1-1-3-4-4-4-2
763.56-1-10-1-4-22
Time (min.)
Lipidm/z
0.25
0.5271530120
573.4900-11-2-1-1
22:3 LPC
574.450000000
575.490122423
22:2 LPC
576.480131132
577.510122223
22:1 LPC
578.500122223
579.520021323
22:0 LPC
580.500111112
581.540122212
Time (min.)
Lipidm/z
0.25
0.5271530120
20:3 LPC
546.39000000-1
547.420010222
20:2 LPC
548.430-131110
549.460011212
20:1 LPC
550.450000102
551.470134323
20:0 LPC,
24:6 LPE
552.450231223
553.470121212
Time (min.)Lipidm/z
0.25
0.5271530120
Lipidm/z
0.25
0.5271530120
Lipidm/z
0.25
0.5271530120
Lipidm/z
0.25
0.5271530120
400.260-1-1-2-1-10628.54-1000000856.6300001011067.87-2-2-2-2-2-2-2
401.330111111629.532323323857.630-10000-11068.870-2-2-2-1-4-4
402.351211012630.590022111858.6200000001069.89-3-2-1-2-2-1-1
403.3100-11011631.580111212859.611-1000001070.86-3-5-4-2-4-5-4
404.386645565632.59-1121111860.62100-310-41071.88-3-4-5-6-5-4-5
405.376656665633.560022113861.63000-2-10-21072.86-1-2-4-3-3-4-3
406.355566555634.53000000-1862.670-3-1-1-1-3-41073.86-2-4-4-3-1-2-3
407.31-10-10000635.500020101863.6500000001074.761-1-1-20-2-2
408.33-2-3-3-1-1-2-2636.54-2-1-3-1-1-1-242:5PC864.6710-20-2-2-11074.990000000
409.32-1000000637.530000000865.650-1-200-2-21075.83-1-2-2-1-1-3-2
410.320001100638.55-4-3-4-2-1-20
42:4PC
42:5PS866.650000-1-101076.841-3-2-1-2-1-2
411.290110001639.53-4-2-2-1-1-20867.6510000001077.870-1-31-1-3-1
412.310000000640.56-2-4-3-3-1-2-142:3PC868.6500000001078.370000000
413.29012022-2641.55-2-3-3-2-1-3-1869.66100-10-1-11078.78-2-3-2-2000
414.300011110642.54-1-4-30-2-2-242:2PC870.670-100-1-101079.1000-1-100-1
415.280-110321643.51-1000110871.690-10-1-1001079.82-2-3-20-2-1-1
416.290-111212644.520000-1-1-142:1PC872.691-1000001080.080000000
417.33-1212223645.510000000873.690-3-2-1-1-3-11080.89-111-211-3
418.330011122646.500000000874.70-2-2-3-2-1-3-11081.91-1-1-1-1-10-2
419.331-10-10-11647.450000000875.70-10-2-1-1-311082.890-1-2-2-1-1-2
420.33-1-1-1-2001648.440000000876.73-1-2-3-41-2-31083.911-2-3-2-2-2-2
421.320-11-1112649.450000000877.74-1-4-4-4-1-4-21084.820-2-2-1-2-2-2
422.34-2-1-10-21126:0PC650.55-2-1-1-3-2-20878.75-1-4-3-4-2-3-21085.14-1-2-1-1-10-1
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38:3PC
38:4PS812.59-2-4-3-3-4-4-41034.2500000001173.02-1-3-2-3-3-3-3
585.52-3-10-1100813.64-1-2-4-3-3-4-31034.78-3-3-4-4-2-3-31173.251111111
586.55-20-1-1-101
38:2PC
38:3PS814.63-2-3-3-2-3-4-21035.76-1-3-2-2-1-2-21173.950-2-2-3-3-2-4
587.55-1-10-1-1-10815.66-1-3-2-3-3-401036.0100000001174.19000-1-100
588.54000-101038:1PC816.65-1-2-1-1-3-311036.8401-1-12-1-21174.87-2-2-4-4-5-4-4
589.510011011817.630-3-3-3-2-3-11037.8300-1-2-1-1-31175.250000000
590.47011211038:1PS818.610-20-10-1-11038.820-1-4-2-1-1-41175.90-1-2-2-2-3-3-2
591.500220211819.611-10-10001039.83-2-4-4-3-2-3-41176.180000000
592.470001121820.610-100-2-2-21040.82-2-4-3-4-4-2-31176.92-2-4-4-3-4-4-4
593.50000-1000821.6000001001041.820-2-1-1-2-1-41177.260000-1-10
594.48-100000042:5PE822.6200-1-100-21042.830-2-1-1-1-1-11178.000-3-4-3-4-4-3
595.490010001823.62000011-11043.82-1-1-2-2-4-2-21178.401110101
596.51-1-110010824.590-1001001044.820-2-1-1-2-2-21179.03-2-3-3-3-4-4-3
597.500000000825.600-1001001045.820-2-3-2-2-2-31179.90-2-4-3-2-4-4-3
598.54-2-4-3-3-2-3-3826.590-1001101046.740-2-2-1-3-2-11180.301-1-1-1-1-10
599.52-1-10-2-200827.620-1001001047.020-1-100-1-11180.94-2-5-5-3-4-4-4
600.55-2-3-4-3-4-40828.610-1001001047.79-1-1-2-1-3-3-31181.260000000
601.520000-1-10829.6300001001048.1900000001181.99-2-5-4-2-5-4-4
602.49000100142:1PE830.6200000001048.83-1-1-2-1-3-2-11182.361111111
603.520224334831.620-1001-101049.75-1-2-2-2-2-2-31183.02-2-3-2-3-2-2-2
604.540121212832.58100001-21050.00-1-1-1-10-101183.280-111111
605.540222224833.581-2-2-1-2-3-31050.84-2-3-3-1-3-2-21184.00-2-3-3-3-2-2-2
606.52123331340:6PC834.591-1-2-1-3-2-21051.830-1-2-1-3-3-21184.270000000
607.480122223835.6311010-111052.8410-1-210-31185.05-1-1-2-3-1-1-1
608.4800-10000
40:5PC
40:6PS836.601-1-2-1-2-2-31053.84-100-31-1-31186.02-2-4-3-4-3-4-4
609.530011001837.6300-1-10-101054.870-2-2-2-1-2-21187.04-3-5-4-4-3-5-3
610.53-4-2-3-1-2-21
40:4PC
40:5PS838.620-1-2-1-1-101055.880-1-2-2-1-1-11188.00-2-3-4-4-4-3-4
611.52-2-1-1-1-111839.6200001001056.800-1-1-1-20-21188.230000000
612.56-2-5-3-1-2-31
40:3PC
40:4PS840.6300-10-2-111057.1300000001188.921012-101
613.55-2-3-3-1-1-11841.6300-1-10-101057.8300-2-21-1-21189.28-1-2-2-2-1-10
614.560-1-2-201040:2PC842.640-1-1-1-2-201058.88-1-4-2-4-1-3-51189.90-1-2-2-3-1-2-2
615.550000000843.6400000-101059.870-2-3-4-4-3-31190.160000000
616.431322133
40:1PC
40:2PS844.650-1-10-1-111060.8200-2-3-2-4-21190.940-2-2-3-3-4-2
617.480100101845.640-1-1-11-101061.830-1-1-4-4-3-31191.241111111
618.51000010040:0PC846.64-1-3-110-101062.83-1-3-4-2-2-3-31191.88-1-2-2-3-3-3-2
619.510000101847.6410000001063.3100000001192.141212222
620.500-10-1-1-20848.68-1-3-4-42001063.84-1-1-1-10-2-21192.40-1-1-1-1-1-1-1
621.490001000849.681-3-2-21-111064.3411111111193.001-1-1-3-2-21
24:0PC622.53-1111110850.70-2-3-3-20-1-11064.85-1-20-1-2-3-21193.991-2-2-3-2-2-1
623.520010111851.7000001001065.3600000001194.990000000
624.53-1-1-2-1-1-11852.690000-1001065.79-2-2-3-3-3-2-21196.000000000
625.520222011853.660-1-100-101066.060-1-100001197.020000000
626.54-1-2-10-3-2-1854.632-1-1-2-1-2-11066.79-2-2-3-2-1-3-31198.030000000
1
2
3
4
5
1
2
3
4
5
Ligand B
Ligand A +
Ligand BLigand A
3x10
6
cellsper
sample
Lipid extraction
45050055060065070075080085090095010001050110011501200
m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Relative Abundance
684.2
773.2
682.3
788.2
794.4
572.3
861.2
885.2
412.1
887.4
820.3
747.1
648.2
536.3
737.3
629.3
478.0
500.1
716.3
620.5
911.3
1175.7
Mass spectra
acquisition
45050055060065070075080085090095010001050110011501200
m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Relative Abundance
684.2
773.2
682.3
788.2
794.4
572.3
861.2
885.2
412.1
887.4
820.3
747.1
648.2
536.3
737.3
629.3
478.0
500.1
716.3
620.5
911.3
1175.7
45050055060065070075080085090095010001050110011501200
m/z
0
5
10
15
20
25
30
35
40
45
50
55
60
65
70
75
80
85
90
95
100
Relative Abundance
684.2
773.2
682.3
788.2
794.4
572.3
861.2
885.2
412.1
887.4
820.3
747.1
648.2
536.3
737.3
629.3
478.0
500.1
716.3
620.5
911.3
1175.7
Lipid Array
generation
Lipidm/z
0.30.52715301200.30.52715301200.30.5271530120
684.481110111000-1-20-10-10-1-1-1-1
685.4811101110000-20-10-10-1-1-1-1
32:2PE
686.48010001100-1-1-3-1-1-1-10-1-1-1-1
687.47-2-2-3-2-3-3-100010100-2-2-1-5-4-3
32:1PE
688.43-5-4-5-4-3-4-30001100-4-4-5-3-5-3-3
689.41-2-3-4-4-2-2-10000200-1-4-3-3-3-2-2
32:0PE
690.41-5-4-5-5-6-6-40000000-5-5-5-5-5-5-4
691.36-1-1-10-10-20000010000000-2
692.39000112100000000000011
693.36000110200000110122231
694.40000222200000001211241
695.40021010110344433434462
696.44011210200000112242212
36:3PA
697.40-1-1-2-2-2-100125542-1033331
698.43-110-10-1-10103431-1101111
36:2PA
699.39-10-2-21-1-101235430-113231
700.400-1-3-3-1-3-1012233111-20002
36:1PA
701.40-2-1-2-2-1-1-1021253300-21101
33:1PE
702.41-3-3-3-4-3-3-2022264200-10-1-10
703.41-3-5-3-3-2-4-20012332-1-1-30-1-2-1
33:0PE
704.42-3-6-4-5-3-4-20000000-5-4-6-4-3-3-2
705.411-2-10-2-2000001001111111
706.41211211000010101222220
Ligand ALigand BLigand A + B
Time (min.)
-6-4-3-202346
Higher SignificanceHigher Significance
Signal DecreasingSignal Increasing
Dual C5a & UDP with Highlights
(Negative Mode Ionization, Seven Data sets)
Lipidm/z
0.25
0.5271530120
882.522010110
38:5PI
883.49-1-3-3-4-2-3-3
884.500-2-2-1-2-2-2
38:4PI
885.512-1-1-5-3-4-4
886.511-1-1-5-4-4-5
38:3PI
887.542-1-2-3-1-2-2
888.541-1-1-4-4-1-2
38:2PI
889.551-1-1-10-10
890.561-1-1-10-11
Time (min.)
Lipidm/z
0.25
0.5271530120
Lipidm/z
0.25
0.5271530120
Lipidm/z
0.25
0.5271530120
Lipidm/z
0.25
0.5271530120
400.204565664629.421233421856.50101012-11070.890-2-300-1-2
401.225465564630.441342423
36:4PI
857.47-1-2-2-2-1-1-11071.380000000
402.234565564631.430221403858.5011000101071.880111101
403.241334131632.483454432
36:3PI
859.48-1-1-2-3-2-201072.401111010
404.201334331633.462243413860.5143332441072.842-2-2-2-10-2
405.204343352634.52-1101120
36:2PI
861.50200-11021073.280000000
406.194534454635.49-1202222862.5120000241073.810-1-2-2-100
16:1LPA
407.182011120636.47-1213243
36:1PI
863.5100101441074.170000000
408.190101132637.425564565864.5201000001074.800-2-2-2-2-1-2
16:0LPA
409.20-2001000638.425564565865.5211320231075.761000000
410.21-1132123639.423555455866.54-10100-101075.9200-100-10
411.211120110640.433455666867.520-100-2-201076.852100000
412.263354555641.424365553868.55200-10-2-11076.980000000
413.214544665642.44-10-12221869.541-1-1-1-10-21077.85-1-1-2-1-1-1-1
414.224333453643.420201221870.570-1-2-1-2-2-21078.22-1-1-1-1-1-1-1
415.203453444644.46-11-12120871.530-2-2-1-2-2-11078.83-10-10010
416.214555666
32:1PA,
22:5LPI
645.42-1121211
40:4PC(Cl)
872.561-1001011079.19-2-2-2-2-2-2-2
417.251212220
C24:1S
646.51-1000-1-10873.541-2-3-4-5-3-31079.820000000
418.241112131
32:0PA,
22:4LPI,
38:2DAG
647.49-1000000874.561-2-2-2-3-201080.18-1-10-1-1-1-1
419.232122342
C24:0S
648.540000001875.560-2-2-2-2-201080.640-10-1-11-2
420.232332341
22:3LPI
649.54-11-11101876.581-1-1-1-1011080.950100011
421.210110110650.52-100-1-100877.5832100021081.25-1-1-1-1-1-10
14:1LPE
422.22012-100-1
22:2LPI
651.472221121878.5710101-101081.77000-1-100
423.231-100000652.440102321879.5700000-101082.260000000
14:0LPE
424.231112340653.402323453880.5410-100-101082.810-2-1-20-3-1
425.232001131654.462232213881.510-2-1-1-2-211083.261011011
426.243534554655.411322310882.5220101101083.8600-100-10
427.235414453656.4602201-11
38:5PI
883.49-1-3-3-4-2-3-31084.340000000
428.233534434657.460221403884.500-2-2-1-2-2-21084.88-1-3-1-5-1-1-2
429.130100021658.4701101-12
38:4PI
885.512-1-1-5-3-4-41085.87-1-3-1-2-1-30
430.140111-111659.4501101-12886.511-1-1-5-4-4-51086.800000011
431.192221341
30:1PE
660.472331212
38:3PI
887.542-1-2-3-1-2-21086.96-2-2-3-1000
432.221123223661.44-1111202888.541-1-1-4-4-1-21087.880-2-2-2-1-30
433.202204241
30:0PE
662.49010-10-12
38:2PI
889.551-1-1-10-101088.840-10-210-1
434.200011110663.453454554890.561-1-1-10-111089.100000000
18:1LPA
435.210111111664.483234444891.5710010021089.80-1-1-2-1-2-20
15:1LPE
436.230101111665.462332333892.5720001101089.970000000
18:0LPA
437.26010021-1665.990-1-10000893.5721101001090.751-2-2-1-1-2-1
15:0LPE
438.260112111666.482222232894.60-10000001091.110000100
439.243332332666.970000000895.6000010001091.811011000
440.262444563667.422243353896.62-1-1-1-10-1-11092.21-2-2-2-2-2-2-2
441.232223322668.4612302-12897.58-1-2-1-1-1-1-11092.930-1-1-1-10-1
442.251334464669.422433343898.58-1-1-1-1-1-1-11093.210000000
443.242223332670.46-10100-11899.56-1-4-3-2-40-21093.810000000
444.261111112
34:2PA
671.39-1-100100900.600-2-1-3-40-11094.14-1-1-1-1-1-1-1
445.261123331672.40-2-1-3-10-11901.580-2-20-2-101094.730000000
446.290012110
34:1PA
673.381121022902.620-1-1-2-20-11095.000-1-1-100-1
447.242223231
C26:1S
674.4321100-11903.64100-1-1211095.8200-100-10
448.250221130
34:0PA
675.420110000904.6911111001096.13-1-10-1-1-1-1
449.240110152
32:0PEe,
C26:0S
676.47-100-1-2-30905.68110121-11096.910-1-100-11
16:1LPE
450.24-1001111677.44-1032112906.6711111001097.920000000
451.240121440678.422232211907.6000-111001098.910-10-2-2-1-1
16:0LPE
452.250000010679.403365543908.591-1100-1-11099.241111111
453.260221343680.4523422-11
40:6PI
909.54-1-2-2-3-3-2-21099.880-100-1-10
454.273423432681.432254323910.540-2-2-3-3-2-31100.39-1000001
14:0LPG
455.251123252682.470-10-1-1-10
40:5PI
911.5400-100-2-11100.8700-1-2-1-1-2
456.253454555683.4700100-10912.54-1-4-1-3-4-3-21101.3400-1-1-1-1-1
457.231113241684.490-10-1-1-1-1
40:4PI
913.5501100111101.85-10-1-2-2-10
458.251124352685.480-10-1-1-1-1914.56-11-200-311102.270010000
459.2800-200-10
32:2PE
686.48-1-10-1-1-1-1
40:3PI
915.570000-1111102.87101000-2
460.2900-210-10687.460-2-2-1-5-4-3916.5800001011103.251012101
461.282413242
32:1PE
688.42-4-4-5-3-5-3-3
40:2PI
917.58000-11221103.862111100
462.271101111689.41-1-4-3-3-3-2-2918.6200000001104.19-2-2-2-2-2-2-2
463.270112231
32:0PE
690.40-5-5-5-5-5-5-4
40:1PI
919.622-2000001104.663333232
17:1LPE
464.260101110691.35000000-2920.6410000001105.13-2-2-3-3-2-3-4
465.260011131692.380000011921.6310000001105.711111010
17:0LPE
466.270000000693.350122231922.65000-10001106.060101100
467.262223330694.391211241923.661-1000201106.680100010
468.27-1002332
36:4PA
695.393434462924.6400000001107.04-1-1-10-1-2-1
469.241101120696.442242212925.621001-1011107.28-2-2-2-2-2-2-2
470.250211221
36:3PA
697.41-1033331926.621-10-10-101107.80110000-1
471.243332421698.43-1101111927.591-10-1-2-101108.200000000
472.271142232
36:2PA
699.400-113231928.560-2-1-1-21-11108.830-10-2000
473.26-101-1001700.4111-20002929.59-1-2-2-2-3-2-31109.340000000
18:3LPE
474.27-1-101110
36:1PA
701.4100-21101930.700-1000011109.87-2-3-3-21-20
475.273354344
33:1PE
702.4100-10-1-10931.6700-10-1-1-11110.420000000
18:2LPE
476.240001111703.42-1-1-30-1-2-1932.7200000001110.910-2-1-20-10
477.252133333
33:0PE
704.42-5-4-6-4-3-3-2933.7100-1010-11111.820000000
18:1LPE
478.240000101705.421111111934.70000-10-101111.940000000
479.250000101706.421222220935.6800-200001112.87-1-10-2-100
18:0LPE
480.26-1-100011707.382344444936.67000-10001113.321111110
16:1LPG
481.240-100010708.451111-1-10937.6400-11-1001113.800000000
482.25-1000010709.424354434938.65000-10-101113.920-1-1-1000
16:0LPG,
26:0DAG
483.24-1001100710.480110-1-12939.632-2-2-1-2-2-21114.390000000
484.250011120711.463453222940.632-4-3-3-3-3-31114.920-2-1-1000
485.230000-11-1
28:0PC(Cl),
34:3PE
712.472431211941.622-1-1-1-2-2-11115.350000000
486.250011121713.441232131942.621-2-4-1-2-101115.93-1-10-2-1-11
487.220111120
34:2PE
714.4000-2-1-200943.62-1-2-2-2-2-101116.421111110
13:0LPC(Cl)
488.350-100112715.40-1-4-4-3-3-1-3944.6320-1-1-1-101116.93-2-3-1-4-20-1
489.290000301
34:1PE
716.42-2-4-5-3-2-3-5945.621-1-1-1-2-101117.24-1-1-1-1-1-1-1
490.290000000717.40-4-2-5-3-3-2-3946.670-1-1-11-2-11117.890-2-1-3-1-2-1
491.281021210
34:0PE
718.42-5-4-5-5-5-5-4947.1300000001118.29-3-2-3-3-3-3-3
19:1LPE
492.28-1-1-1-1000
32:1PG
719.40-1-101-210947.6421-1-1-1001118.8900-1-10-10
493.271102221720.40-1-101-111948.681-1-10-1-101119.224334443
16:1LPS,
19:0LPE
494.260000000
32:0PG
721.390244321949.6810000001119.8401-10000
495.261102-1-1-1
36:4PEp
722.411102110950.69201-10-101120.221111111
16:0LPS
496.271103-10-1
38:4PA
723.411155562951.59010-10-211120.83-1-1-1-1000
497.27010511-1724.432113330952.6000001-211121.352222211
20:5LPE
498.24-1-10-1-100
38:3PA
725.42-1-234442953.59000-22-201121.79-1-1-2-1-2-1-1
499.241111011726.42-1-1-20101954.6000-1-22-301122.090000000
20:4LPE,
14:1LPC(Cl)
500.23-1-10-1001
38:2PA
727.4200-11011955.6311010001122.8000000-10
501.25-1-11-1001728.44-1-1-3-1-1-11956.6610-1-10-1-11122.980-10-1000
20:3LPE,
14:0LPC(Cl)
502.25-1-11-1001729.44-2-3-20000957.7000101001123.400000000
503.26-1-11-1-111
35:1PE
730.45-3-4-5-3-3-2-2958.71-1-2-2-2-1-101123.930-100-1-10
20:2LPE
504.28-1-11-1-101731.43-1-3-5-2-4-1-2959.7000-1-1-1-1-11124.371-10-1000
505.280-10-1001
32:1PS
732.41-2-2-2-3-3-2-2960.7310000-201124.990111111
20:1LPE
506.29-1-2-1-1-101733.40-2-1-2-1-20-1961.7110000001125.86000-1000
18:2LPG
507.28-1-1-1-1-100
32:0PS
734.43000-10-20962.70100-11-1-11126.290-1-1-1-10-1
20:0LPE
508.34-2-1-10111735.4210001-1-1963.6810-100-101126.9200-10000
18:1LPG
509.260000001
30:2PC(Cl),
36:5PE
736.421-100-1-3-2964.7311-100001127.751000000
510.270000000737.433011211965.6910000-1-11127.950000000
18:0LPG,
28:0DAG
511.29-1011011
36:4PE,
30:1PC(Cl)
738.433121211966.71100-1-1-1-11128.83110-1010
512.28-1-112032739.433021211967.64000-1-2-2-21129.232122312
513.270022032
36:3PE,
30:0PC(Cl)
740.422-1-11100968.63000-10-101129.920000000
15:1LPC(Cl)
514.270001121741.42-1-2-4-2-2-1-1969.65000-1-1-1-11130.562112221
515.281111111
36:2PE
742.44-1-2-3-210-1970.6600-1-10-2-11130.970001100
15:0LPC(Cl)
516.310000002743.43-1-2-3-2-2-1-1971.650-1-1-10-2-11131.28-3-3-2-3-3-3-3
517.310100110
36:1PE
744.44-2-4-6-3-2-2-4972.69100-1-10-11131.961-1-1-1-1-1-1
518.43-110-1000
34:2PG
745.42-2-1-1-2-2-2-1973.6610-1-10-101132.290000000
519.41-1100111
38:6PEp
746.42-1-10-1-2-10974.6812000001132.9400-1-2-10-1
18:2LPS
520.350001111
34:1PG
747.42112-1111975.671-2-1-10001133.48-1-1-1-1-10-1
521.31-21-11001
38:5PEp
748.430011001976.682-1-2-2-20-11133.91-10101-2-1
18:1LPS
522.300000000
34:0PG
749.430011011977.6521-1-10-101134.321201-111
523.310000000750.4400001-1-1978.7020-1-1-21-31134.94-11-2-1302
18:0LPS,
22:6LPE
524.250000101751.4400-1011-2979.67100-1-1001135.90000-1000
525.260000101
31:1PC(Cl)
752.4510-2-1-1-11980.691-100-1001136.210000000
22:5LPE
526.25-1-11-1001753.4500-11111981.6810-1-10001136.94-2-2-2-2000
527.26-1-11-1-100
31:0PC(Cl),
38:3PEe
754.452-3-4-3-2-2-2982.7010000001137.390000000
22:4LPE,
16:1LPC(Cl)
528.26-1-11-1001755.45-1-4-5-2-3-3-1983.6910-10-1-1-11137.95-1-1000-10
529.26-1001011756.46-2-2-5-3-3-2-2984.7210-2-1-2-1-11138.92-2-10-2001
22:3LPE,
16:0LPC(Cl)
530.260001113757.45-2-3-5-2-4-1-3985.741-1-1-2-2-1-11139.281000010
20:4LPG
531.270012132
34:2PS
758.45-1-2-3-1-10-1986.7601-1-10001139.89-10-10000
22:2LPE
532.290000003759.44-2-1-3-1-300987.72100-2-1-1-11140.340000000
20:3LPG
533.280010112
34:1PS
760.430121222988.7321-111121140.76-1010111
22:1LPE
534.360000001761.44-1021121989.732-1-1000-11140.940000000
20:2LPG
535.34-1-110112
34:0PS,
38:6PE
762.440011011990.730-2-1-2-10-11141.350000000
C16:0S
536.4501-11121763.450012121991.7011001001141.900-1-3-4-30-1
20:1LPG
537.440000000
38:5PE,
32:2PC(Cl)
764.4410-200-10992.74110-20001142.500000000
538.43-2000022765.4511-1-1100993.71100-1-1-2-11142.940-1-20-200
30:0DAG
539.41-1000110
32:1PC(Cl),
38:4PE
766.442-100100994.2200000001143.84-1-1-1-1-11-1
540.36-1101233767.442-1-100-1-1994.71-1-1-1-1-1-101144.07-1-2-2-2-2-1-1
541.311013320
32:0PC(Cl),
38:3PE
768.442-1-2-2-3-2-1995.72-1-10-10-201144.87-1-10-1-100
542.30-1131234
36:4PG
769.4410-4-4-3-4-2996.71000-10001145.090000000
543.281212230
38:2PE
770.46-3-3-5-4-3-6-4997.7310012001145.840-1-1-1-1-1-1
20:4LPS,
17:0LPC(Cl)
544.33-2253324
36:3PG
771.45-10-1-1-2-2-1998.74-1-1-1-1-1-101146.190-1-1-10-10
545.350131220772.46-2-3-2-2-5-4-4999.6900-10-1-101146.830-20-1-1-1-2
20:3LPS
546.35-1-100011
36:2PG
773.4522210111000.731-1-1-3-30-11147.150000000
547.330112220
40:6PEp
774.4522210001001.711-2-1-10-1-11147.900000000
20:2LPS
548.32-1000111
36:1PG
775.4612221101002.691-10-10-1-11148.49-1-1001-1-2
549.320123142776.4711201101003.6900-1-1-1001149.011010100
20:1LPS
550.410021233777.4833432451004.2000000001149.571111111
551.381314352
33:2PC(Cl)
778.4733332321004.721-200-1001149.99-100-1-1-10
20:0LPS,
18:3LPC(Cl)
552.34-1000101779.4823223321005.69100-10001150.580-11-1-1-1-1
553.34-1020244
33:1PC(Cl)
780.4721000001006.7000-3-3-2-2-21150.980100002
18:2LPC(Cl)
554.364444444781.4720-112-101007.701100-1001151.440000000
22:6LPG
555.333455554
33:0PC(Cl),
36:4PS
782.491-1-2-1-3-2-21008.72000-10-1-11151.96-100-1-1-1-1
18:1LPC(Cl)
556.302223323783.49-1-3-1-2-3-3-21009.7321000-2-11152.390000000
22:5LPG
557.290123243784.48-1-1-3-1-10-11010.750-1-2-2-1-1-21152.970111221
18:0LPC(Cl)
558.340012123785.481-1-2-1-2-1-21011.7711010001153.300000000
22:4LPG
559.351113123
36:2PS
786.4601222301012.811-10-3-2-1-31153.900001001
560.320102011787.4701310111013.80-101-10-1-21154.330000000
22:3LPG
561.331243334
36:1PS
788.4600021111014.8101011111154.900-1-1-2011
562.382365465789.4701122101015.790001-1001155.81-10-1-1-1-1-1
22:2LPG
563.381333454
36:0PS,
40:6PE
790.4701111001016.7910-1-3-2001155.950-20000-1
C18:0S
564.44-11-11111791.470-1112001017.75-1-3-3-4-4-3-41156.76-10-1-1-1-10
32:1DAG
565.42-1112222
34:2PC(Cl)
792.452-11-100-11018.7221-1000-11156.950000000
566.400100121793.472-100-1-2-31019.2600000001157.420000000
32:0DAG
567.393453463
34:1PC(Cl)
794.481-100-1-101019.7211100-111157.83000-1-1-10
22:6LPS
568.280011224795.4821101001020.1900000001158.21-1-1-1-1-1-1-1
16:1LPI
569.290121131
34:0PC(Cl)
796.4820101001020.75-1-1-3-2-1-3-21158.830000-100
22:5LPS,
19:1LPC(Cl)
570.27-1002435
38:4PG
797.48-1-1-1-1-1-1-21021.710-1-1-11-201159.100000000
16:0LPI
571.280021221798.49-1-2-1-2-3-3-31022.73-1-1-1-10-1-11159.8900-10-100
22:4LPS,
19:0LPC(Cl)
572.421221202
38:3PG
799.49-1000-2001023.71000-10-201160.25-1-100-100
573.421231203800.49-1-100-4-2-11024.73-10-2-2-1-1-21160.96-1-100000
22:3LPS
574.421231203
38:2PG
801.50-1011-2-111025.7300-1-1-1-101161.380000000
575.420220203802.500-111-1011026.75-100-2-1-1-11161.94-10-100-10
22:2LPS
576.41-1230203803.501-111-2001027.77-1-1-1-10-2-11162.330000000
577.392443543804.5022100141028.79-1-3-2-3-2-101162.96-1-10-1-1-10
22:1LPS,
20:4LPC(Cl)
578.393464666805.4811322331029.76-1-3-3-2-1001163.93-2-1-200-10
579.393434565
35:2PC(Cl),
38:6PS
806.4732333341030.78-1-20-22101164.9600-10001
20:3LPC(Cl)
580.351142424807.4742446641031.1700000001165.370000000
581.353333443
35:1PC(Cl),
38:5PS
808.5122132421031.740-1-10-1-201165.960-100-1-10
20:2LPC(Cl)
582.310-110312809.5122233311032.75-2-1-3-1-11-11166.420-1-10-1-1-1
583.322023434
38:4PS
810.5301122211033.2200000001166.87-1-100-100
20:1LPC(Cl)
584.360000002811.5301120011033.75100000-11167.180000000
585.361011113
38:3PS
812.4901210211034.741-1-100-1-11167.810001110
586.430-200011813.5001111221035.7610000001168.09-1-10-1-10-1
587.410142132
38:2PS
814.490-1110111036.8021011001168.92-10-11101
588.391222312815.4911210111037.8010000001169.360000000
589.402463344
38:1PS,
36:4PC(Cl)
816.5001011101038.821-1-1-1-1-1-11169.91-1-1-10-3-2-1
590.450221233817.481-1100111039.821-1-10-1-1-21170.440000000
34:2DAG
591.443144342
36:3PC(Cl)
818.51-1-20-10-201040.84010-10111170.892111111
C20:0S
592.47-1221232819.4912221001041.8300-1-1-2101171.310000000
34:1DAG,
18:3LPI
593.455563655
36:2PC(Cl)
820.492-11-10-101042.84000-1-1111171.91-10-1-1-10-1
594.432464566
40:6PG
821.49200-1-1-1-21043.841-1-100-101172.681111000
34:0DAG,
18:2LPI
595.374364555
36:1PC(Cl)
822.502-11-10-101044.8200000111172.960000000
596.333435464
40:5PG
823.5020102-1-11045.750-2-1-2-10-11173.9000000-10
18:1LPI
597.271123322824.502-11-11-2-11046.761-1-3-2-1011174.90000-1000
598.330122445
40:4PG
825.5012101-201047.73-1-1-1-10001175.900000-1-10
18:0LPI
599.321144443826.50-1-1-1-1-1-1-11048.6600-10-1-101176.910000-100
600.412341423827.5100-10-3-3-31048.81001-1-20-21177.91-11-1-1-1-1-1
601.401241423828.51-1-101-1011049.2000000001178.92-10-1-1-1-1-2
22:6LPC(Cl)
602.411232413829.51101-1-2-1-11049.780-1-20-1-2-11179.94-1-1-2-1-10-1
603.410222403830.5121111111050.81-1-2-1-1-3-1-21180.96-200-10-11
22:5LPC(Cl)
604.411323523831.5011001001051.771-3-2-2-1-101181.950000000
605.402463524
40:7PS
832.5044545551052.2000000001182.98-100000-1
606.450332352
34:2PI
833.4721116561052.790-3-4-4-30-21183.980000000
607.442322324
40:6PS,
37:2PC(Cl)
834.4843444421053.760-1-2-1-2-1-21184.990000100
22:3LPC(Cl)
608.400134434
34:1PI
835.4831435551054.78-2-1-1-2-2-1-21186.00-10-1-1-1-1-1
609.375563665
40:5PS
836.4821333321055.780-2-2-1-2001186.380000000
610.364443653
34:0PI
837.490-1221121056.2600000001186.932110000
611.375564665
40:4PS
838.5001433231056.83-1-2-1-11-1-11187.320000000
612.392432334839.5000110001057.8100000001187.991101100
613.393565662
38:6PC(Cl),
40:3PS
840.5121202101058.810-3-3-2-2-301188.771110010
614.392343341841.5110111111059.2300000001189.100011000
615.401243443
38:5PC(Cl)
842.5211212211059.8000000001189.93-1-1-1-2-1-1-1
616.410102222843.5210121101060.81000-10001190.94-11-1001-1
30:1PA,
20:5LPI,
36:3DAG
617.423453444
38:4PC(Cl),
40:1PS
844.550-10-2-2-2-21061.79-1-1-10-1001191.920000000
C22:1S
618.470132313845.541-1-1-1-1-3-11062.3500000001192.920000000
20:4LPI,
30:0PA,
36:2DAG
619.410142434
38:3PC(Cl)
846.572-11-10-2-11062.84011-1-1001193.940100111
C22:0S
620.51-1000001847.571-10-10-101063.88010000-11194.950000-100
20:3LPI,
36:1DAG
621.47-1001001848.571-100-1-201064.9111011101195.970-100100
622.47-1000000849.581-10-10-111065.84-11-110001196.980000000
20:2LPI
623.411232134850.57100-10-101066.2200000001197.99001-1-1-1-1
624.404464556851.571-2-1-1-1-2-11066.86-1-100-1-101198.420000000
20:1LPI
625.394565666852.5420-1-10-1-11067.8500-1-2-2-211198.87-1-101-1-20
626.451241223853.52-1-2-1-2-1-3-11068.88-10-1-1-1011199.04000-1000
627.422344533854.5210000001069.3900000001199.650000000
628.452253332855.51-2-1-2-10-201069.890110011
Lipidm/z
0.25
0.5271530120
36:4PA
695.393434462
696.442242212
36:3PA
697.41-1033331
698.43-1101111
36:2PA
699.400-113231
700.4111-20002
36:1PA
701.4100-21101
33:1PE
702.4100-10-1-10
703.42-1-1-30-1-2-1
33:0PE
704.42-5-4-6-4-3-3-2
705.421111111
Time (min.)
Lipidm/z
0.25
0.5271530120
38:4PA
723.411155562
724.432113330
38:3PA
725.42-1-234442
726.42-1-1-20101
38:2PA
727.4200-11011
728.44-1-1-3-1-1-11
729.44-2-3-20000
35:1PE
730.45-3-4-5-3-3-2-2
731.43-1-3-5-2-4-1-2
32:1PS
732.41-2-2-2-3-3-2-2
733.40-2-1-2-1-20-1
Time (min.)
Lipidm/z
0.25
0.5271530120
34:2DAG
591.443144342
C20:0S
592.47-1221232
34:1DAG,
18:3LPI
593.455563655
594.432464566
34:0DAG,
18:2LPI
595.374364555
596.333435464
Time (min.)
Lipidm/z
0.25
0.5271530120
30:1PA,
20:5LPI,
36:3DAG
617.423453444
C22:1S
618.470132313
20:4LPI,
30:0PA,
36:2DAG
619.410142434
C22:0S
620.51-1000001
20:3LPI,
36:1DAG
621.47-1001001
Time (min.)
Lipidm/z
0.25
0.5271530120
32:0PC(Cl),
38:3PE
768.442-1-2-2-3-2-1
36:4PG
769.4410-4-4-3-4-2
38:2PE
770.46-3-3-5-4-3-6-4
36:3PG
771.45-10-1-1-2-2-1
772.46-2-3-2-2-5-4-4
36:2PG
773.452221011
Time (min.)
1
2
3
4
5
6
1
2
3
4
5
6
Dual C5a & UDP with Highlights
(Positive Mode Ionization, Seven Data sets)
Lipidm/z
0.25
1271530
120
Lipidm/z
0.25
0.5271530120
Lipidm/z
0.3
0.5271530120
Lipidm/z
0.25
0.5271530120
400.2700000-10630.581113012860.640-2-3-21-1-31067.87-3-1-4-4-3-4-1
401.331111111631.580011122861.6300-201-2-11068.86-2-3-2-3-3-3-3
402.342113232632.601112123862.66-1-3-4-4-4-2-41069.82-1-100-1-2-1
403.3101-1001-1633.561112222863.65-10000001070.090000000
404.396664455634.530-10202042:5PC864.67-10-1-2-2-101070.86-2-2-4-4-3-3-4
405.376666555635.51-10-1-1010865.650-1-2-1-1-3-21071.87-2-3-4-4-4-2-4
406.355555564636.54-4-1-20-111
42:4PC
42:5PS866.6400-10-2-101072.87-1-3-4-4-2-2-3
407.31-1000-1-10637.530001110867.661000-3-101073.84-3-4-4-4-2-3-4
408.33010-1010638.56-1-2-3-1-1-1042:3PC868.6600-20-3-3-11074.290000000
409.320100010639.53-3-3-30001869.6810-10-2-1-21074.83-2-2-3-3-2-3-3
410.310001110640.56-3-4-3-2-30-142:2PC870.67-10-1-20-3-11075.84-1-2-2-10-2-2
411.291100-110641.55-1-2-20-100871.6800-1-1-1-101076.84-1-2-2-2-1-2-2
412.310101111642.54-2-3-2-20-1042:1PC872.690-1-2-10-201077.860-2-2-2-1-2-2
413.291000000643.52-1000000873.680-1012-211078.270000000
414.300000220644.520-20-10-2-1874.700-2-2-1-2-3-21078.77-2-1-1-2-1-1-2
415.280010220645.5100001-10875.700-2-2-2-3-3-21079.100000000
416.290110211646.51-1000100876.74-2-3-4-3-5-5-41079.81-2-2-2-3-2-3-3
417.321323242647.4500000-10877.73-3-3-4-3-5-3-41080.0600-1-1000
418.330032112648.4300000-10878.75-2-4-4-3-5-5-41080.90-1-4-1-10-2-3
419.32-1-2-22-2-10649.4500000-10879.76-3-3-4-4-4-5-41081.91-1-4-4-2-1-2-5
420.33-2-2-10-2-1026:0PC650.55-1-1-1-1-2-2-2880.70-2-3-4-2-3-4-41082.89-1-3-4-4-3-3-5
421.320001010651.540000000881.67-1-3-4-2-4-5-51083.84-1-1-2-2-2-2-2
422.34-30-10-110652.5200-10010882.660-3-3-1-3-3-31084.080000000
423.34000-1-1-10653.512223224883.640-2-10-1-1-11084.83-1-2-3-4-3-3-3
14:1LPE424.35-2-2-2-3-3-20654.531-1-10-100884.660-2-1-1-2-1-21085.140000000
425.3301-11110655.511000-20-1885.62-10000001085.7620-10-1-2-1
14:0LPE426.340-10-100-1656.510-101000886.641100-1-2-11086.150000000
427.34-12-110-2-1657.49-2-1-1010-1887.61-1-1-2-2-4-3-11086.88-1-3-3-5-3-3-4
428.34-3-2-30-2-2-1658.510-1-1-10-1-1888.67-1-3-5-2-2-4-21087.81-1-2-2-3-1-4-4
429.300011110659.51-1-2-100-2-1889.650-1-1-2-3-3-11088.04-1-20-2-2-1-2
430.33-1000-100660.4900-10110890.70-3-2-3-2-3-3-11088.760-2-1-2-2-2-2
431.220343343661.490-1000-1-1891.69-3-2-4-4-3-4-31089.100-1-2-10-10
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