Lim et al, Supplemental Table S1. Primer list for cloning and semi-quantitative RT-PCR.
4
Prim erNam e Locus N um ber Forw ard Prim er R everse Prim ers G ene Specific Prim erforR T-PC R O s 18S rR NA F+R O s09g00999 5'--ATG ATAAC TC G AC G G ATC G C -3' 5'-CTTG G ATG TG GTAG C C G TTT-3' OsRFPH2-1 F+R O s05g51780 5'-AG C ACC G C ATCG TG G TGTC G-3' 5'-GAG TC AAC C ACC G G G G C AG C -3' OsRFPH2-2 F+R O s02g45390 5'-C C ACG GATC TCG AC C GC ACG -3' 5'-TGTG AAG C C GG G C TG C TTTC T-3' OsRFPH2-3 F+R O s01g74040 5'-TC C CC TG G CTC GC TCTC C AC -3' 5'-AGC AATG G C ATCG G G G TG G C -3' OsRFPH2-4 F+R O s01g20910 5'-ATG G G C G C TG G C G AAC AC AA-3' 5'-GG TCG AAG CG AAG AC C C G C C -3' OsRFPH2-5 F+R O s05g08610 5'-TC AC G AC G C G C C C AAC TTG T-3' 5'-ACC CTGTTG TCG C TG TG G C G -3' OsRFPH2-6 F+R O s04g49160 5'-C G TC G C G C TG TTC AAG C TG C -3' 5'-GC CTACTG CC AAG C AG G C C C -3' OsRFPH2-7 F+R O s06g12560 5'-G G C C TC TC G C G C G TATTG G T-3' 5'-TG TTG C C TAC ATAC AG G G C AC G AG -3' OsRFPH2-8 F+R O s01g11520 5'-C G TCAC C C GC G TC TTGC TGT-3' 5'-CG TTG ACC TGC C AC C G G C TT-3' OsRFPH2-9 F+R O s03g04890 5'-TG C TG C TC G ATC TG C G G C TG -3' 5'-ATGG C GGG AG GTTG G G AG G T-3' OsRFPH2-10 F+R O s08g42640 5'-G C C C ACAC CTC GC AACG G AA-3' 5'-TG G C TC TG C G G C ATTC G C TC -3' OsRFPH2-11 F+R O s10g26610 5'-TG G G G TC ATTG TG C TG C G TC G -3' 5'-C AG C C AC C G AG AC TC G C ATG T-3' OsRFPH2-12 F+R O s01g58780 5'-TC C TCAG G C AAGG C C GC AGA-3' 5'-AACG G GTG CTG C C TG AAC G G -3' OsRFPH2-13 F+R O s07g22840 5'-TG G C ATG G G G TG G C TTC TTG G -3' 5'-G C AC ATTG G G C AAAG TG G C G TA-3' OsRFPH2-14 F+R O s04g48260 5'-G C TC G AG AG C TG C AAC AG ATG G A-3' 5'-TG C C C G AC C C ATAAAC C TC C T-3' OsRFPH2-15 F+R O s02g40664 5'-G C AGC AG C TGAG AG G GG TGC -3' 5'-GTTTGTC G G AGC TG C G G C C T-3' OsRFPH2-16 F+R O s11g41140 5'-AG C TGG G AC AGTG C AC GC AG-3' 5'-TC C AC AAAC AG G G C ATG C TTC AC -3' OsRFPH2-17 F+R O s07g29600 5'-C C G G C G CG GTC TTCTC C ATC -3' 5'-CG GG CATG ATG C G TG C C TG A-3' OsRFPH2-18 F+R O s12g39110 5'-G C ACG GG CTC C AG CTC AAGG -3' 5'-TC G AG G C AG G C C G AG TG G AA-3' OsRFPH2-19 F+R O s03g57500 5'-G C G AC AAC G ACC CC TCC ACG -3' 5'-GC ATG CAG GG AG C C AC C AG G -3' OsRFPH2-20 F+R O s05g47670 5'-AC C AG G ATTG G C G AC TC G AC G -3' 5'-TG G C C AC AG ATC AG C C TC C C T-3' OsRFPH2-21 F+R O s06g01200 5'-C TG CG GTG GAG GC TTCG TCG -3' 5'-GTC GAGCC AG GG G AG G ATG C -3' OsRFPH2-22 F+R O s02g54624 5'-TG G TG C AC C TAG TG C AG G AC AT-3' 5'-CAG CAGAAG AAC G C C C G C C A-3' OsRFPH2-23 F+R O s01g49770 5'-G C AG C C TTATC AAAG G TAG C G AG T-3' 5'-CTG TG GCC G CAATC C AG C C T-3' OsRFPH2-24 F+R O s05g47780 5'-C C AG AC C C C TTC TTG C AATG G G C -3' 5'-TCTG C GGC C AGC AAG C C ATC -3' OsRFPHC-1 F+R O s03g44810 5'-TG G TGAC C AG CAG G AG GG GA-3' 5'-TC AG C C AAG G G G AC TG C AAC A-3' OsRFPHC-2 F+R O s08g04130 5'-C G G TG GTG GTC ACC GTC G TG -3' 5'-CC TCG ACG ATC G G C G C G AAA-3' OsRFPHC-3 F+R O s02g19804 5'-ATC C GG C TC TCC GC TCG G TT-3' 5'-TTGC G GATC TCC TG TG C C G C -3' OsRFPHC-4 F+R O s07g46700 5'-AC G CC AAC TC GC TTGC TGC T-3' 5'-TC G G AC C G TC G C TATG TC C AC T-3' OsRFPHC-5 F+R O s09g33740 5'-TG C C G TTTATG C C TG G C G C A-3' 5'-AGC CC ACC C CAAC G TG G G TA-3' OsRFPHC-6 F+R O s07g43380 5'-AG AC G G C C AG G G C AAC ATC A-3' 5'-GC AGC CG TAG CAG G AC C TG TG -3' OsRFPHC-7 F+R O s01g74320 5'-C C TG C AC G G C AG C G AC ATC A-3' 5'-AG C C G C G G C C TATC C TG G AA-3' OsRFPHC-8 F+R O s05g33000 5'-C AG CAG G C GC G G AAGG TG AA-3' 5'-AC AG C TG TG TC C C TTG G C TC TG T-3' OsRFPHC-9 F+R O s02g03620 5'-TG C TG G C G ATG TTC C G ATG C T-3' 5'-CC AGATGC GG CC G G TAAC C C -3' OsRFPHC-10 F+R O s10g36880 5'-TG C GC TG C GAG TAC AAC CC G-3' 5'-GC CG ATGC TC TTC AC C G G C A-3' OsRFPHC-11 F+R O s05g01230 5'-C C G G G G TGG GC GG AC TC TAA-3' 5'-TC C C C C TTG C G C TTC C TTG C -3' OsRFPHC-12 F+R O s04g43300 5'-AC C TGC C C CG TCTG CC TG AA-3' 5'-C G G C AG G G TC C C TTC TG C AC -3' OsRFPHC-13 F+R O s07g47590 5'-G G TC AC AAG AG C C ATG G G G C A-3' 5'-CTG CC TGTCG GC AC AG TG G G -3' OsRFPHC-14 F+R O s01g13370 5'-G G G TG C AC AAC C G TTG C C C T-3' 5'-TTGC G CCG AC CTC AAG G C TG -3' OsRFPHC-15 F+R O s05g19480 5'-C C C TC TC C CTC CG CC TC C AC-3' 5'-ATGG G CGC TG CC C G TTC ATC -3' OsRFPHC-16 F+R O s09g39690 5'-C G TC G TC AG G G G TC ATG C AG T-3' 5'-TC G C TG TG C C AC TC G TTG TG T-3' OsRFPV-1 F+R O s01g19800 5'-TG C GG ATG C TCTG G C TCC CT-3' 5'-CTG GTGTG CAG C TC C G AC C G -3' OsRFPV-2 F+R O s05g28730 5'-C C C C C TG TGG ATGC TCTG GC -3' 5'-AC ATTAATTAC AAG C TG C TG G TG C TG C -3' OsRFPV-3 F+R O s01g63150 5'-AC G ACC TC TC CTG C G CC C AT-3' 5'-GC TGAGCG AC GTG G TG AC C C -3' OsRFPV-4 F+R O s01g42500 5'-C G C AG CAG TTCG TG C ATCG C-3' 5'-GG ACG GC TAC GG TTG C AG C G -3' OsRFPV-5 F+R O s06g46366 5'-TG C TG TC AC AC G C AC TG G C A-3' 5'-TC G ATG C TG C G C TTG C TG TC T-3' OsRFPV-6 F+R O s02g03950 5'-TG C AC TG AG TG TC G G G C TG C -3' 5'-AG C C C C AG AATC C TC AG C TC TG T-3' OsRFPV-7 F+R O s01g66970 5'-G G CG C C ATGG ATTC C CC C TG -3' 5'-ACC TG TCTTC GC C TC C G G TG T-3' Lim et al, Supplemental Table S1. Primer list for cloning and semi-quantitative RT-PCR
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Transcript of Lim et al, Supplemental Table S1. Primer list for cloning and semi-quantitative RT-PCR.
Primer Name Locus Number Forward Primer Reverse Primers
Gene Specific Primer for RT-PCR
Os18SrRNA F+R Os09g00999 5'--ATGATAACTCGACGGATCGC-3' 5'-CTTGGATGTGGTAGCCGTTT-3'OsRFPH2-1 F+R Os05g51780 5'-AGCACCGCATCGTGGTGTCG-3' 5'-GAGTCAACCACCGGGGCAGC-3'OsRFPH2-2 F+R Os02g45390 5'-CCACGGATCTCGACCGCACG-3' 5'-TGTGAAGCCGGGCTGCTTTCT-3'OsRFPH2-3 F+R Os01g74040 5'-TCCCCTGGCTCGCTCTCCAC-3' 5'-AGCAATGGCATCGGGGTGGC-3'OsRFPH2-4 F+R Os01g20910 5'-ATGGGCGCTGGCGAACACAA-3' 5'-GGTCGAAGCGAAGACCCGCC-3'OsRFPH2-5 F+R Os05g08610 5'-TCACGACGCGCCCAACTTGT-3' 5'-ACCCTGTTGTCGCTGTGGCG-3'OsRFPH2-6 F+R Os04g49160 5'-CGTCGCGCTGTTCAAGCTGC-3' 5'-GCCTACTGCCAAGCAGGCCC-3'OsRFPH2-7 F+R Os06g12560 5'-GGCCTCTCGCGCGTATTGGT-3' 5'-TGTTGCCTACATACAGGGCACGAG-3'OsRFPH2-8 F+R Os01g11520 5'-CGTCACCCGCGTCTTGCTGT-3' 5'-CGTTGACCTGCCACCGGCTT-3'OsRFPH2-9 F+R Os03g04890 5'-TGCTGCTCGATCTGCGGCTG-3' 5'-ATGGCGGGAGGTTGGGAGGT-3'OsRFPH2-10 F+R Os08g42640 5'-GCCCACACCTCGCAACGGAA-3' 5'-TGGCTCTGCGGCATTCGCTC-3'OsRFPH2-11 F+R Os10g26610 5'-TGGGGTCATTGTGCTGCGTCG-3' 5'-CAGCCACCGAGACTCGCATGT-3'OsRFPH2-12 F+R Os01g58780 5'-TCCTCAGGCAAGGCCGCAGA-3' 5'-AACGGGTGCTGCCTGAACGG-3'OsRFPH2-13 F+R Os07g22840 5'-TGGCATGGGGTGGCTTCTTGG-3' 5'-GCACATTGGGCAAAGTGGCGTA-3'OsRFPH2-14 F+R Os04g48260 5'-GCTCGAGAGCTGCAACAGATGGA-3' 5'-TGCCCGACCCATAAACCTCCT-3'OsRFPH2-15 F+R Os02g40664 5'-GCAGCAGCTGAGAGGGGTGC-3' 5'-GTTTGTCGGAGCTGCGGCCT-3'OsRFPH2-16 F+R Os11g41140 5'-AGCTGGGACAGTGCACGCAG-3' 5'-TCCACAAACAGGGCATGCTTCAC-3'OsRFPH2-17 F+R Os07g29600 5'-CCGGCGCGGTCTTCTCCATC-3' 5'-CGGGCATGATGCGTGCCTGA-3'OsRFPH2-18 F+R Os12g39110 5'-GCACGGGCTCCAGCTCAAGG-3' 5'-TCGAGGCAGGCCGAGTGGAA-3'OsRFPH2-19 F+R Os03g57500 5'-GCGACAACGACCCCTCCACG-3' 5'-GCATGCAGGGAGCCACCAGG-3'OsRFPH2-20 F+R Os05g47670 5'-ACCAGGATTGGCGACTCGACG-3' 5'-TGGCCACAGATCAGCCTCCCT-3'OsRFPH2-21 F+R Os06g01200 5'-CTGCGGTGGAGGCTTCGTCG-3' 5'-GTCGAGCCAGGGGAGGATGC-3'OsRFPH2-22 F+R Os02g54624 5'-TGGTGCACCTAGTGCAGGACAT-3' 5'-CAGCAGAAGAACGCCCGCCA-3'OsRFPH2-23 F+R Os01g49770 5'-GCAGCCTTATCAAAGGTAGCGAGT-3' 5'-CTGTGGCCGCAATCCAGCCT-3'OsRFPH2-24 F+R Os05g47780 5'-CCAGACCCCTTCTTGCAATGGGC-3' 5'-TCTGCGGCCAGCAAGCCATC-3'OsRFPHC-1 F+R Os03g44810 5'-TGGTGACCAGCAGGAGGGGA-3' 5'-TCAGCCAAGGGGACTGCAACA-3'OsRFPHC-2 F+R Os08g04130 5'-CGGTGGTGGTCACCGTCGTG-3' 5'-CCTCGACGATCGGCGCGAAA-3'OsRFPHC-3 F+R Os02g19804 5'-ATCCGGCTCTCCGCTCGGTT-3' 5'-TTGCGGATCTCCTGTGCCGC-3'OsRFPHC-4 F+R Os07g46700 5'-ACGCCAACTCGCTTGCTGCT-3' 5'-TCGGACCGTCGCTATGTCCACT-3'OsRFPHC-5 F+R Os09g33740 5'-TGCCGTTTATGCCTGGCGCA-3' 5'-AGCCCACCCCAACGTGGGTA-3'OsRFPHC-6 F+R Os07g43380 5'-AGACGGCCAGGGCAACATCA-3' 5'-GCAGCCGTAGCAGGACCTGTG-3'OsRFPHC-7 F+R Os01g74320 5'-CCTGCACGGCAGCGACATCA-3' 5'-AGCCGCGGCCTATCCTGGAA-3'OsRFPHC-8 F+R Os05g33000 5'-CAGCAGGCGCGGAAGGTGAA-3' 5'-ACAGCTGTGTCCCTTGGCTCTGT-3'OsRFPHC-9 F+R Os02g03620 5'-TGCTGGCGATGTTCCGATGCT-3' 5'-CCAGATGCGGCCGGTAACCC-3'OsRFPHC-10 F+R Os10g36880 5'-TGCGCTGCGAGTACAACCCG-3' 5'-GCCGATGCTCTTCACCGGCA-3'OsRFPHC-11 F+R Os05g01230 5'-CCGGGGTGGGCGGACTCTAA-3' 5'-TCCCCCTTGCGCTTCCTTGC-3'OsRFPHC-12 F+R Os04g43300 5'-ACCTGCCCCGTCTGCCTGAA-3' 5'-CGGCAGGGTCCCTTCTGCAC-3'OsRFPHC-13 F+R Os07g47590 5'-GGTCACAAGAGCCATGGGGCA-3' 5'-CTGCCTGTCGGCACAGTGGG-3'OsRFPHC-14 F+R Os01g13370 5'-GGGTGCACAACCGTTGCCCT-3' 5'-TTGCGCCGACCTCAAGGCTG-3'OsRFPHC-15 F+R Os05g19480 5'-CCCTCTCCCTCCGCCTCCAC-3' 5'-ATGGGCGCTGCCCGTTCATC-3'OsRFPHC-16 F+R Os09g39690 5'-CGTCGTCAGGGGTCATGCAGT-3' 5'-TCGCTGTGCCACTCGTTGTGT-3'OsRFPV-1 F+R Os01g19800 5'-TGCGGATGCTCTGGCTCCCT-3' 5'-CTGGTGTGCAGCTCCGACCG-3'OsRFPV-2 F+R Os05g28730 5'-CCCCCTGTGGATGCTCTGGC-3' 5'-ACATTAATTACAAGCTGCTGGTGCTGC-3'OsRFPV-3 F+R Os01g63150 5'-ACGACCTCTCCTGCGCCCAT-3' 5'-GCTGAGCGACGTGGTGACCC-3'OsRFPV-4 F+R Os01g42500 5'-CGCAGCAGTTCGTGCATCGC-3' 5'-GGACGGCTACGGTTGCAGCG-3'OsRFPV-5 F+R Os06g46366 5'-TGCTGTCACACGCACTGGCA-3' 5'-TCGATGCTGCGCTTGCTGTCT-3'OsRFPV-6 F+R Os02g03950 5'-TGCACTGAGTGTCGGGCTGC-3' 5'-AGCCCCAGAATCCTCAGCTCTGT-3'OsRFPV-7 F+R Os01g66970 5'-GGCGCCATGGATTCCCCCTG-3' 5'-ACCTGTCTTCGCCTCCGGTGT-3'
Lim et al, Supplemental Table S1. Primer list for cloning and semi-quantitative RT-PCR
Lim et al, Supplemental Table S1. (Continued from previous page.)
Primer Name Locus Number Forward Primer Reverse Primers
Ubiquitination Assay
pMAL-c5X-OsRFPH2-2 F+R Os02g45390 5'-GAGAGAAGGATTTCAATGCTGGGGTCGGGGCTGAATC-3' (XmnI) 5'-GAGAGTCGACTCAGTGCATTTCTTGTTGGATTTCAATGAC-3' (SalI)pMAL-c5X-OsRFPH2-3 F+R Os01g74040 5'-GAGAGAAGGATTTCAATGGACGACGACGCCTCCTCCTACA-3' (XmnI) 5'-GAGAGGATCCTCATCTAGTAGCAATGGCATCGGGGTGG-3' (BamH)pMAL-c5X-OsRFPH2-14 F+R Os04g48260 5'-GAGAGAAGGATTTCAATGGAGGTGATGCATGATACCACTG-3' (XmnI) 5'-GAGAGGATCCTCACTTGCCCGACCCATAAACCTCCTTGTT-3' (BamHI)pMAL-c5X-OsRFPH2-16 F+R Os11g41140 5'-GAGAGAAGGATTTCAATGGGAGCTCTTTGTTCATGTTTGC-3' (XmnI) 5'-GAGAGTCGACTTATGTTGTCTCGTCAAACTCCATTTTCTTTCC-3' (SalI)pMAL-c5X-OsRFPH2-23 F+R Os01g49770 5'-GAGAGAAGGATTTCAATGGTGGGGAGAGGCCGTGG-3' (XmnI) 5'-GAGAGTCGACTCAAGTCTTGGTAACGGCAGTCTTGCAAACT-3' (SalI)pMAL-c5X-OsRFPHC-2 F+R Os08g04130 5'-GAGAGAAGGATTTCAATGGGCGGCGTGATCGGAGCGTTAT-3' (XmnI) 5'-GAGAGGATCCTCATCCTGAGTACAGCCTCAGCTTGGACC-3' (BamHI)pMAL-c5X-OsRFPHC-3 F+R Os02g19804 5'-GAGAGAAGGATTTCAATGGCCGACGGCGGCGGCGGCGG-3' (XmnI) 5'-GAGAGTCGACCTATTGCTTCTTGTCTTGTGCCATCTTCTT-3' (SalI)pMAL-c5X-OsRFPHC-13 F+R Os07g47590 5'-GAGACATATGATGAAGTTTGCCAAGAAGTACGAGAAGTA-3' (NdeI) 5'-GAGAGGATCCTCACATGCCCAAGAATGCCCTGCACTGTGAC-3' (BamHI)pMAL-c5X-OsRFPV-6 F+R Os02g03950 5'-GAGAGAAGGATTTCAATGATTTCAGTATTGTTCATAATGC-3' (XmnI) 5'-GAGAGGATCCTCAATATAGCCCCAGAATCCTCAGCTCTGT-3' (BamHI)
Subcellular localization studies
35S::OsRFPH2-3-EGFP F+R Os01g74040 5'-GAGAGGATCCATGGACGACGACGCCTCCTCC-3' (BamHI) 5'-GAGAGGTACCTCTAGTAGCAATGGCATCGGG-3' (KpnI)35S::OsRFPH2-14-EGFP F+R Os04g48260 5'-GAGATCTAGAATGGAGGTGATGCATGATACC-3' (XbaI) 5'-GAGAGGTACCCTTGCCCGACCCATAAACCTCCT-3' (KpnI)35S::OsRFPH2-16-EGFP F+R Os11g41140 5'-GAGATCTAGAATGGGAGCTCTTTGTTCATGT-3' (XbaI) 5'-GAGAGGTACCTGTTGTCTCGTCAAACTCCATTTTCT-3' (KpnI)35S::OsRFPH2-23-EGFP F+R Os01g49770 5'-GAGATCTAGAATGGTGGGGAGAGGCC-3' (XbaI) 5'-GAGACCCGGGAGTCTTGGTAACGGCAGTCTTGCA-3' (SmaI)35S::OsRFPHC-2-EGFP F+R Os08g04130 5'-GAGATCTAGAATGGGCGGCGTGATCGGAGCG-3' (XbaI) 5'-GAGAGGTACCTCCTGAGTACAGCCTCAGCTTG-3' (KpnI)35S::OsRFPHC-3-EGFP F+R Os02g19804 5'-GAGATCTAGAATGGCCGACGGCGGCGGCG-3' (XbaI) 5'-GAGAGGTACCTTGCTTCTTGTCTTGTGCCATCT-3' (KpnI)35S::OsRFPHC-4-EGFP F+R Os07g46700 5'-GAGATCTAGAATGGGCAGGTCGTTCAGGGACT-3' (XbaI) 5'-GAGAGGTACCCAACGAGCAACAGCATCGTAC-3' (KpnI)35S::OsRFPHC-13-EGFP F+R Os07g47590 5'-GAGATCTAGAATGAAGTTTGCCAAGAAGTACGAGAAGTA-3' (XbaI) 5'-GAGAGGTACCCATGCCCAAGAATGCCCTGCACTGTGAC-3' (KpnI)35S::OsRFPV-6-EGFP F+R Os02g03950 5'-GAGATCTAGAATGATTTCAGTATTGTTCAT-3' (XbaI) 5'-GAGAGGTACCATATAGCCCCAGAATCCTCAGCT-3' (KpnI)
Yeast Two-Hybrid Assays
pGBKT7-OsRFPH2-3 F+R Os01g74040 5'-GAGACATATGATGGACGACGACGCCTCCTC-3' (NdeI) 5'-GAGACCCGGGTCATCTAGTAGCAATGGCAT-3' (SmaI)pGBKT7-OsRFPH2-14 F+R Os04g48260 5'-GAGACCCGGGGATGGAGGTGATGCATGATAC-3' (SmaI) 5'-GAGACTGCAGTCACTTGCCCGACCCATAAA-3' (PstI)pGBKT7-OsRFPH2-16 F+R Os11g41140 5'-GAGACCCGGGGATGGGAGCTCTTTGTTCATG-3' (SmaI) 5'-GAGACTGCAGTTATGTTGTCTCGTCAAACT-3' (PstI)pGBKT7-OsRFPH2-23 F+R Os01g49770 5'-GAGACCCGGGGATGGTGGGGAGAGGCCGTGG-3' (SmaI) 5'-GAGACTGCAGTCAAGTCTTGGTAACGGCAG-3' (PstI)pGBKT7-OsRFPHC-2 F+R Os08g04130 5'-GAGAGAATTCATGGGCGGCGTGATCGGAGC-3' (EcoRI) 5'-GAGAGGATCCTCATCCTGAGTACAGCCTCA-3' (BamHI)pGBKT7-OsRFPHC-3 F+R Os02g19804 5'-GAGACATATGATGGCCGACGGCGGCGGCGG-3' (NdeI) 5'-GAGACCCGGGCTATTGCTTCTTGTCTTGTG-3' (SmaI)pGBKT7-OsRFPHC-13 F+R Os07g47590 5'-GAGACTGCAGTCACATGCCCAAGAATGCCC-3' (PstI) 5'-GAGACTGCAGTCACATGCCCAAGAATGCCC-3' (PstI)pGBKT7-OsRFPV-6 F+R Os02g03950 5'-GAGACTGCAGTCAATATAGCCCCAGAATCC-3' (PstI) 5'-GAGACTGCAGTCAATATAGCCCCAGAATCC-3' (PstI)
Lim et al, Supplemental Table S2. The predicted subcellular distributions of 120 positive interactors of the 7 OsRFPs
Gene name Chloroplast Cytoskeleton Cytosol E.R. Extra cellular Mitochondria Nucleus Plasma membrane Vacuole Total
OsRFPH2-3 8 (34.78%) 0 (0.00%) 5 (21.74%) 0 (0.00%) 3 (13.04%) 3 (13.04%) 2 (8.70%) 1 (4.35%) 1 (4.35%) 23
OsRFPH2-14 11 (37.93%) 1 (3.45%) 7 (24.14%) 0 (0.00%) 1 (3.45%) 1 (3.45%) 8 (27.59%) 0 (0.00%) 0 (0.00%) 29
OsRFPH2-16 9 (34.62%) 0 (0.00%) 5 (19.23%) 0 (0.00%) 1 (3.85%) 1 (3.85%) 8 (30.77%) 1 (3.85%) 1 (3.85%) 26
OsRFPH2-23 6 (26.09%) 1 (4.35%) 8 (34.78%) 1 (4.35%) 3 (13.04%) 1 (4.35%) 2 (8.70%) 0 (0.00%) 1 (4.35%) 23
OsRFPHC-2 2 (25.00%) 0 (0.00%) 3 (37.50%) 0 (0.00%) 1 (12.5%) 0 (0.00%) 2 (25.00%) 0 (0.00%) 0 (0.00%) 8
OsRFPHC-13 3 (42.86%) 0 (0.00%) 1 (14.29%) 0 (0.00%) 0 (0.00%) 1 (14.29%) 1 (14.29%) 1 (14.29%) 0 (0.00%) 7
OsRFPV-6 11 (35.48%) 0 (0.00%) 5 (16.13%) 0 (0.00%) 1 (3.23%) 1 (3.23%) 9 (29.03%) 4 (12.90%) 0 (0.00%) 31
Overlapped genes 6 0 11 0 1 1 7 0 1 27
Total 44 (36.7%) 2 (1.7%) 23 (19.2%) 1 (0.8%) 9 (7.5%) 7 (5.8%) 25 (20.8%) 7 (5.8%) 2 (1.7%) 120
Lim et al, Supplemental Table S3. Expression values of 7 OsRFPs and 112 interactors in the context of drought, salt, heat, and cold stresses.
Probe ID Genes Drought Salt Heat Cold Probe ID Genes Drought Salt Heat Cold Probe ID Genes Drought Salt Heat ColdOs.996.1.S1_at Os01g01620 1.559183013 0.936478924 -0.325592437 0.410828635 Os.19421.1.S1_at Os02g58570 1.105616117 0.729407905 0.045225288 -0.119161063 Os.14584.1.S1_at Os06g36360 0.402348385 0.576166217 -0.786454742 0.63002137
Os.12788.1.S1_at Os01g11240 0.210232721 0.045271901 0.168169 -0.199538978 Os.9533.1.S1_at Os03g02750 0.11214598 0.651008771 0.007915204 0.935661923 Os.14584.1.S1_s_at Os06g36360 0.169522007 0.415204046 -0.74322527 0.121440718
Os.34283.2.S1_x_at Os01g12160 -0.110720872 -0.095071739 -1.216251168 0.029157843 Os.2217.1.S1_a_at Os03g06620 -0.61137651 -0.29507614 0.15135255 0.50892422 OsAffx.15775.1.S1_at Os06g36360 0.541312603 0.099523505 -0.738008822 0.366879309
Os.34283.3.S1_at Os01g12160 -0.7237018 -0.879038549 -1.454552542 -0.072892542 OsAffx.25019.1.S1_at Os03g08380 -0.257634457 -0.157928504 0.026869839 -0.129142719 Os.12027.1.S1_a_at Os06g38210 -0.122616877 0.005053077 -0.70446329 0.140098985
Os.3507.1.S1_at Os01g12200 0.645124262 0.585081634 -1.175467961 -0.472222932 Os.5477.1.S1_at Os03g11320 0.149552165 -0.103224296 0.108563408 0.204827347 Os.52817.1.S1_at Os06g38210 -0.220795492 -0.21412733 -0.665092391 -0.084773767
OsAffx.9908.1.S1_x_at Os01g12200 0.570672695 0.607792205 -1.180016527 -0.262164188 Os.5477.1.S2_at Os03g11320 -0.039676374 -0.137824951 0.124936903 0.151323028 Os.7771.1.S1_at Os06g43600 1.036446144 0.697055199 0.106233984 -0.497696602
Os.872.1.S1_at Os01g14590 0.0624323 -0.091172542 -0.89095081 0.061138117 Os.1376.1.S1_at Os03g11540 -1.983324405 -1.779125679 -0.15238105 -0.390741735 Os.166.1.S1_at Os06g43660 0.09001926 0.24155685 -0.19684198 -0.13760095
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Os.37869.2.S1_a_at Os01g15270 1.11507747 1.08431846 1.68564943 0.71588356 Os.10732.1.S1_s_at Os03g19380 -0.96000244 -0.26300209 0.96436404 -0.30394864 Os.50189.2.S1_at Os06g46910 -0.207456007 0.048274105 0.135972212 -0.1534325
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Os.4671.1.S1_a_at Os01g24710 0.85678709 1.04599588 2.522971468 0.44443957 Os.46231.1.S1_a_at Os03g46770 0.14651646 0.21449256 0.33719186 0.19026231 Os.37287.1.S1_at Os06g51290 -0.311863639 -0.076625695 0.131435618 0.705414778
Os.57456.1.S1_x_at Os01g24710 1.28776559 1.24597764 2.568409136 0.65308561 Os.46231.2.S1_x_at Os03g46770 0.16379681 0.17609161 0.29375826 0.13919412 Os.7931.1.S1_a_at Os06g51290 -0.788466394 -0.414237055 -0.354119284 -1.187237106
Os.38249.1.S1_at Os01g38530 0.973479756 0.32213703 0.192854582 1.269120307 Os.3420.1.S1_at Os03g50885 -0.18165879 -0.39475479 0.602371577 -0.01668027 Os.7931.1.S1_s_at Os06g51290 -0.822190391 -0.312357496 -0.418727465 -1.175186381
Os.38249.2.S1_at Os01g38530 1.54194497 1.177965012 -0.23254973 0.901625144 Os.3420.1.S1_s_at Os03g50885 -0.53236379 -0.76764033 0.30143717 -0.12334092 Os.12627.1.S1_at Os07g04840 -1.17583111 -0.32852966 0.02979529 -0.28220451
Os.9499.1.S1_at Os01g46980 0.38673048 0.10225227 0.05837255 0.14223476 Os.272.1.S1_at Os03g51030 -0.363557802 -0.07033472 -0.303835919 0.362451569 Os.12274.1.S1_at Os07g06590 -0.27027667 -0.39784113 0.48819088 -0.19425716
Os.13615.1.S1_at Os01g47262 1.793620294 1.504501079 0.823523557 0.32029273 Os.47842.1.A1_s_at Os03g51030 -0.198033073 0.05249261 0.023948126 0.22440876 Os.52047.1.S1_at Os07g17184 -0.188787497 -0.172309512 2.413932836 0.062264971
Os.13615.4.S1_x_at Os01g47262 1.764124839 1.397574783 0.624530275 0.284943156 Os.11437.1.S1_at Os03g53860 0.489461081 0.442275631 2.53337428 0.052118405 Os.52047.1.S1_x_at Os07g17184 -0.104519489 -0.038696458 1.802824067 0.029564859
OsAffx.11501.1.S1_x_at Os01g47262 1.615000638 1.160355673 0.61684188 0.166753803 Os.5502.1.S1_at Os03g57790 -0.10085287 0.05302394 -0.70839253 0.33589099 Os.52047.2.S1_at Os07g17184 -0.223273537 -0.345207727 1.312506423 -0.268063003
Os.31693.2.S1_at Os01g48830 0.878598896 0.329166277 -0.872347881 0.572206385 Os.8586.1.S1_at Os03g57790 -0.278506296 -0.467200986 0.128049133 -0.306901673 Os.11330.1.S1_a_at Os07g26690 0.32028146 0.28616581 1.14177065 -0.09900312
Os.31693.3.S1_at Os01g48830 0.880300992 0.561765028 -0.268677267 -0.039669265 Os.44934.1.S1_at Os03g63310 -0.221959146 0.077027577 0.634364516 -0.084710565 Os.11330.1.S1_x_at Os07g26690 0.31670739 0.31301261 1.10794032 -0.14826734
Os.31693.4.S1_x_at Os01g48830 0.716338633 0.648057743 -0.311888156 0.113098012 Os.9293.1.S1_at Os04g40040 1.124022601 0.532843144 -0.044068718 0.850886169 Os.5937.1.S1_at Os07g29290 0.018330056 0.22254414 -0.630837481 -0.353879851
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Os.11450.1.S1_at Os01g52110 2.810063252 2.045431872 0.32654188 0.847102505 Os.20424.1.S1_s_at Os04g46100 -0.468790928 -0.041916933 -0.377696526 -0.213195332 Os.49200.2.S1_at Os07g32600 -0.500173748 -0.076558916 0.223984152 -0.38613005
Os.11450.1.S2_a_at Os01g52110 3.363184644 2.488359414 0.56492095 0.519411531 OsAffx.14289.1.S1_at Os04g46100 -0.123376591 0.407294119 -0.300361476 -0.286068386 Os.13599.1.S1_at Os07g33240 -0.088032111 -0.421147553 1.008907023 0.136590841
Os.11450.1.S3_a_at Os01g52110 2.576533788 1.895445588 0.63353401 0.696234978 Os.17316.1.S1_at Os04g49194 -0.110322968 -0.003266907 -1.030425079 0.46199974 Os.22277.1.S1_at Os07g34520 3.562032715 4.705922371 -2.53495697 0.255722656
Os.27065.1.S1_a_at Os01g59180 0.892361544 0.647994253 0.059496435 0.156431001 OsAffx.4131.1.S1_at Os04g49670 -0.204365242 -0.177508647 0.033999012 0.072808754 Os.27293.1.S1_a_at Os07g36600 1.597334991 1.431759201 -0.018898949 0.258937853
Os.27065.1.S1_at Os01g59180 0.504806301 0.416705884 0.093560915 -0.224128914 Os.20283.1.S1_at Os04g49900 0.207000552 0.809598701 0.016397797 0.41768519 Os.27293.1.S1_at Os07g36600 1.98842824 1.374730266 -0.121468972 0.222850005
Os.27065.2.S1_x_at Os01g59180 0.628335632 0.609590647 -0.107026122 0.072198055 Os.17232.1.S1_at Os04g51300 -1.135407596 -0.737321794 1.393869079 -0.421155518 Os.27293.1.S1_s_at Os07g36600 1.55002385 1.2463414 0.107663335 0.18163794
Os.8999.1.S1_at Os01g67860 0.87842417 0.76382945 0.34277234 0.60608878 Os.17232.1.S2_at Os04g51300 -1.034364813 -0.53380919 1.436592866 -0.36601233 Os.27293.2.S1_at Os07g36600 2.851304458 3.31178803 0.991284592 1.463753399
Os.8999.3.S1_x_at Os01g67860 0.61766162 0.66639972 0.132522052 0.46704487 Os.27519.1.S1_at Os04g51300 -0.405846856 -0.057624172 -0.036948445 -0.169480693 Os.12624.1.S1_s_at Os07g37240 -1.69361043 -0.35478374 0.13110346 -0.53935234
Os.11975.2.A1_at Os01g68300 -0.33044368 -0.07282593 -1.16376188 -0.02430119 Os.14095.1.S1_at Os04g52450 0.9353479 0.91519423 -0.114630004 0.156839896 Os.10733.1.S1_at Os07g38130 2.521657383 2.143453303 -0.561015754 -0.860610252
Os.50999.1.S1_at Os01g69090 0.012018805 -0.135306667 -0.395436959 -0.410600407 OsAffx.26573.1.S1_x_at Os04g52450 1.093815857 1.031464047 -0.336605126 0.274168787 OsAffx.5593.1.S1_at Os07g41390 -0.034301925 -0.09195238 -0.157506389 -0.073151066
Os.42975.1.S1_x_at Os01g73730 0.038719522 -0.059144055 -0.06230415 0.035233016 Os.27395.1.S1_a_at Os04g57760 2.969303816 2.514292386 -0.620629494 1.229690952 Os.7362.1.S1_at Os07g48550 2.286769406 2.378807386 -1.517487142 0.743071879
Os.12410.1.S1_a_at Os01g74300 -0.18685319 0.09894491 -1.05208233 0.09871498 OsAffx.14458.1.S1_x_at Os04g57760 2.506650433 1.961974343 -0.862527907 0.813580772 Os.52466.1.S1_at Os08g07280 -0.026593667 -0.011575364 -0.768374901 0.38066067
Os.12410.1.S1_x_at Os01g74300 -0.25568833 0.01361577 -1.258695 0.1005953 Os.12918.1.S1_s_at Os04g58320 0.787116574 0.635341184 -1.528155304 -0.336530005 Os.10193.1.S1_at Os08g09200 0.168851505 0.211846648 -0.17620891 -0.175048368
Os.12410.3.S1_s_at Os01g74300 -0.31205484 -0.06371894 -1.35481242 0.02286777 Os.12918.2.S1_x_at Os04g58320 0.917345138 0.685489559 -1.265028233 -0.406474634 Os.17629.1.A1_at Os08g23150 0.651664142 0.396061116 0.641735348 0.073210083
Os.12410.3.S1_x_at Os01g74300 -0.39711324 -0.03253051 -1.11207623 0.04106301 Os.27339.1.S1_at Os05g01540 -0.31722877 -0.216436034 0.333969016 -0.532629524 OsAffx.17147.1.S1_at Os08g25420 -0.000698819 -0.012824271 -0.069041342 0.114054259
Os.51720.1.S1_at Os02g01500 -0.712272938 -0.823013495 -0.036405361 -0.125863647 Os.12791.1.S1_a_at Os05g02070 -0.72931773 -0.14002146 -1.21610586 0.02217899 Os.21893.3.A1_at Os08g31980 1.306781003 1.745127261 1.571317426 2.067428084
Os.52219.1.A1_at Os02g04640 2.499708512 3.416844649 -0.829005207 -0.091705405 Os.12791.2.S1_a_at Os05g02070 -0.59108284 -0.04760816 -1.19212794 0.07134107 Os.5444.1.S1_at Os08g39090 0.678353523 0.41802707 -0.091722467 0.164858647
Os.37946.1.S1_at Os02g05830 -1.430366794 -0.125849702 -0.357296487 -0.000974712 Os.12791.4.S1_x_at Os05g02070 -0.77139611 -0.12931937 -1.31107673 0.07105956 Os.5444.1.S2_at Os08g39090 0.505608598 0.36062552 -0.192841041 0.013033524
Os.22660.1.S1_x_at Os02g06640 0.74720718 0.74900334 0.07937674 0.05232694 Os.14153.3.S1_x_at Os05g02070 -1.11197985 -0.15201894 -1.24791377 0.07208581 Os.14318.1.S1_at Os08g42470 2.779982558 1.81248269 -0.052549269 1.099489946
Os.22660.3.A1_at Os02g06640 0.35534718 0.846668019 -0.035567614 0.532146769 Os.11045.1.S1_s_at Os05g07690 0.44473113 0.292110693 0.634074632 0.345969895 Os.55221.1.S1_at Os08g43120 3.771477739 2.072109228 -0.871235149 0.566427378
Os.22660.3.S1_x_at Os02g06640 0.73957367 0.67248878 0.08252865 0.06971617 Os.11045.2.S1_s_at Os05g07690 0.279392341 0.250747445 0.592220289 0.271071231 Os.12127.1.S1_at Os09g30340 -1.63202437 -0.5075121 0.883130407 -0.40028379
Os.57479.1.A1_at Os02g06640 0.23981246 0.210173255 -0.372291962 0.078307472 Os.35448.1.S1_at Os05g07690 0.428593217 -0.140420154 -0.126067395 -0.297210987 Os.12127.2.A1_at Os09g30340 -0.178684229 0.891483062 0.171504184 0.452552835
Os.57479.1.A1_x_at Os02g06640 0.133165721 0.256029203 -0.38994705 -0.113041163 Os.1382.1.S1_at Os05g11710 -0.67648262 -0.54526522 1.03193892 0.03291267 Os.12127.2.S1_x_at Os09g30340 -1.40033572 -0.27097065 0.38149686 -0.21181479
Os.57479.1.S1_x_at Os02g06640 0.86741201 0.8525566 -0.08525921 0.16333159 Os.37717.1.A1_s_at Os05g15770 2.196332544 1.700750994 4.982289257 0.757721724 Os.19234.1.S1_s_at Os09g30340 1.470077561 1.597344535 0.156505087 1.107992366
OsAffx.11931.1.S1_at Os02g07800 -0.352732485 0.327686889 -0.213715922 0.206322921 Os.37717.1.A2_s_at Os05g15770 1.49894634 1.11776181 4.757154249 0.49525126 Os.22948.1.S1_s_at Os09g30340 0.933867046 1.077544823 -0.098662937 0.638136684
Os.13494.1.S1_at Os02g09150 -1.027891882 -0.49589713 0.23993666 -0.20247891 Os.11773.1.S1_at Os05g27730 1.214806778 1.208276628 -0.85376053 1.159156898 Os.25439.1.S1_at Os10g13800 0.753478024 0.244186002 -0.207305129 0.314574645
Os.8901.1.S1_at Os02g22130 0.139053501 0.043999646 -0.140084523 1.095647726 Os.22312.3.A1_a_at Os05g28740 0.55104236 1.1390497 -1.38918077 0.35486953 Os.25439.2.S1_at Os10g13800 -0.85946262 -0.220983705 -0.676461408 -0.05926062
Os.8901.1.S2_at Os02g22130 -0.258886362 -0.10087787 -0.15849664 0.718859423 Os.28077.1.S1_at Os05g34070 0.85309429 0.42343752 1.0101864 0.21624511 Os.25439.2.S1_x_at Os10g13800 -0.908442777 -0.294747743 -0.880902161 -0.028972291
Os.17451.1.S1_at Os02g26294 -0.005322806 -0.091682297 0.171457925 0.23488829 Os.15216.1.S1_at Os05g36190 0.792387688 0.728381128 -0.19192209 -0.20688581 Os.25167.1.S1_a_at Os10g25674 -1.057991171 0.16122894 0.399348805 -0.197673299
OsAffx.12271.1.S1_at Os02g29390 0.21262522 0.076733843 -0.161251732 0.166246141 Os.12561.1.S1_at Os05g39960 -0.110954112 -0.323638402 1.31220533 0.34820457 OsAffx.30758.1.S1_at Os11g01060 -0.062781123 -0.009056721 -0.039205737 0.03436017
Os.9390.1.S1_at Os02g32750 0.181578327 -0.143366825 0.030980948 -0.037100655 Os.10152.1.S1_at Os06g05880 0.07697279 0.05504146 -0.20504846 0.12886785 Os.4902.1.S1_at Os11g16330 0.12000427 0.430888401 0.032689283 0.427139824
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Os.37752.1.S1_at Os02g44630 -0.27974859 0.09023735 1.78716706 -0.31868937 Os.4905.4.S1_a_at Os06g10770 0.082403442 0.200560918 1.184012031 -0.121252457 Os.25589.3.S1_at Os11g25780 1.52336703 1.15169564 1.62332581 -0.6435864
Os.313.1.S1_a_at Os02g49720 2.805732335 1.956433552 -0.497030835 0.709854895 Os.4905.4.S1_at Os06g10770 -0.191565733 0.077892461 0.97670462 -0.089138637 Os.25589.3.S1_x_at Os11g25780 1.244442022 1.174299582 1.602210711 -0.536007306
Os.313.2.S2_at Os02g49720 0.302608752 0.029301496 -0.081425292 0.247556762 Os.27584.1.S1_at Os06g12040 -0.300972604 -0.033898055 0.435377058 -0.222056389 Os.20289.1.S1_at Os11g37960 -1.335832597 0.057530832 2.270324655 0.07002641
Os.12178.1.S1_at Os02g52290 -0.51669794 -0.24872203 -0.21047024 0.02957367 OsAffx.27834.1.S1_at Os06g27510 0.060683892 0.110960916 -0.370327702 0.206696423 Os.8178.1.S1_at Os11g37970 0.547214481 0.057211741 0.558279516 0.490416009
Os.54944.1.S1_at Os02g52670 4.53576801 3.965700771 -1.520119705 3.271039297 OsAffx.15760.1.S1_at Os06g35870 -0.008484362 -0.232410403 -0.138300865 -0.159154734 Os.8943.1.S1_at Os11g40510 -0.53183876 -0.21890589 -0.74069965 0.06882977
