Light Microscopy
Bright Field
Fluorescence
Phase-Contrast
Differential Interference Contrast (DIC)
Electron Microscopy (EM)
Scanning EM
Transmission EM
Chemical Composition of Cells
Pg. 58
Chemical Bond Energies
Nitrogenous Bases of DNA and RNA
Pg. 76
0.34 nm
Pg. 171
Major Groove
Minor Groove
SupercoiledDNA
Non-supercoiledDNA (relaxed)
Werner Syndrome
15 yrs. 48 yrs.
Mutation in gene encoding RecQ helicase
Human Karyotype
Pg. 179
RNA Molecules
5’ 5’
3’3’
(Pgs. 74-75)
Amino Acid Side Chains
Extra carbon atom!
ß-Sheet
alpha-helix
Hydrophobic Interactions in Tertiary Structure
Representations of Protein Tertiary Structure
Backbone
Ribbon
Wire Frame
Space Filling
Pg. 132
Quaternary Structure
Pg. 141
Chloride Ion Channel Protein
Comparison of Protein Primary Structure
Cyclin-dependent Kinase Proteins
Gene Expression
Pg. 230
Pg. 202
RNASynthesis
OH
OH
OH
Exon/Intron Organization of Eukaryotic Genes
Pg. 238
Sequence Structure of Introns
Pg. 241
Alternative Splicing
Pg. 237
5’ Guanine Cap Structure
CytosolNucleusMitochondria
SecretedERGolgiEndosomeLysosomeTransmembrane
Reading Frames in RNA
tRNA Synthetase
tRNA Amino Acid
Synthetase(blue)
EditingDomain
“Editing” by tRNA Synthetase
Large Subunit RNA Secondary Structure
Small Ribosomal Subunit
Pg. 253
3’
mRNA Structure
Pg. 253
Initiation of Translation
tRNA
Release Factor (RF)
Co-translational Protein Folding
Protein Folding by “Chaperones”
TranscriptionalActivator
TranscriptionalRepressor
Pg. 257
Ubiquitin-dependent Protein Degradation
Proteasome
Amino acids + Ub
Protein Ub-Ub-Ub
Protein + Ub
Ubiquitin Ligase
Coding and catalysis
Coding
Coding intermediate
Superficial Infections Systemic Infections
Fungal Disease