Facilitating tissue collection for translational research

Jane Rogan

Business Manager

MCRC Biobanking: The Basic Concept

Make quality tissue collection and retrieval easy for the researcher

• Regulatory Framework

• Sample Collection Logistics

• Sample Quality

• Informatics

• Sample Access

Collaborating Trusts

MCRC Biobank

Pennine Acute

Hospitals Trust

The Christie NHS

Foundation Trust

South Manchester

NHS Foundation

Trust

Salford Royal NHS Foundation

Trust

HPB Breast

Colorectal Skin Urology Gynaecology Haematological Malignancies

Lung Breast Head & Neck Colorectal

Breast Upper GI Urology Neurology

Building a successful Biobank

3%

15%

1%

7%

11%

11%

1%

18%

33%

Collections By Disease Type - Solid Tumours

Brain Breast Sarcoma Skin Colorectal

Upper GI Gynae Urology Lung

Donations from over

6000 patients

47%

11%

10%

32%

Collections By Disease Type - Blood Disorders

AML B-ALL MDS Other

Donations from 400 patients

Biobank Project Applications

1

13

5

4

5

2

11

6

3

1

4

8

23

Gynae

Breast

Colorectal

Upper GI

GU

Lymphoma

Haematology

Lung

Head and neck

Neuro

Melanoma

Mixed Sample Types

QC/Method Development

0 5 10 15 20 25

Total Applications: 86 Total Approved: 75

Average Approval Time: 30 working days (median)

The “Six-Pack”

1. Fresh Frozen Diseased Tissue

2. Fresh Frozen Non-involved Tissue

3. FFPE Disease Tissue

4. FFPE Non-involved Tissue

5. Pre-operative Blood sample

6. Pre-operative Urine sample

Melanoma Tissue Collection Pathway

Collect Fresh From Theatres

Fresh Cut-Up in Pathology Diagnostic Sample Research Sample

Mouse model of tumour

Frozen Tissue for Nucleic Acid Extraction

Fixed Tissue for Staining

Evolving the Collections

Requirement Considerations Prospective Fresh Tissue • Availability of tissue

• Staff available for sample delivery

• Media for keeping samples viable

Use for Animal Models • Specific consent for animal models

• Size of tumour tissue

Alternative Sample Types (fluids, plucked hair)

• Routes / logistics of sample collection

Serial Blood Collection inc CTCs • Biobank Technician presence in clinic

Past samples (e.g. pre-treatment/primary tumours)

• Specific consent

• Requesting blocks from local hospitals

Sampling tumour in different areas • Further input from pathology

Clinical Trial Sample Collection • Space for sample storage

• Different protocols for each trial

Biobank Projects by Type

17

39

10

8

Fresh Tissue

Banked Samples

Archive FFPE/TMA

Prospective Protocol

Total Approved Projects By Type

0

2

4

6

8

10

12

14

16

Fresh Tissue

Banked Samples

Archive FFPE/TMA

Prospective Protocol

Active Projects By Type (Total 33*)

Tissue Microarrays

• Current disease areas include prostate, bladder, lung • Link to clinical databases • Recently obtained Section 251 Support which allows the Biobank

to link archival samples to clinical data • Support through Surgical Oncology Research Theme – Data

Manager employed

External Projects utilising Biobank Resource

• Stratified Medicine Programme – Phase 2 – Lung focus

– Biopsy samples from late stage patients

• 100k Genome Project – Lung, Colorectal, Prostate

• TracerX – Facilitating sample collection

• MesoBank – Mesothelioma

Research Biopsies

• Working with clinical teams to introduce sample collection as a research only procedure

• How tumours change over time

• Comparing primary and metastatic tumours in patients

• Research into why patients are relapsing on targeted treatments

• Pilot to being in Malignant Melanoma - 2014

Key Success Drivers

• Established network model with existing infrastructure and pathways for sample collection

• Excellent clinical engagement across all cancer disease types

• Robust governance framework for tissue and data

• Proven track record of providing quality samples

• Research needs drive the sample collection, not the other way round

Thank you

http://www.mcrc.manchester.ac.uk/biobank/