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Fig. 3. Image of a characterist~c ection of a manne snow particle prepared by Treatment 2: (A ) a TEM micrograph and [B ) acomputerized 3-dimensional reconstruction based on serial sections. Associations between bacteria and mineral particles andfibrils binding different kinds of subcomponents of the particle together ar e visible. Scale bar = 0.5 pm(measurements of dimensions and shapes) of single generally true for preparations in which Nanoplast@ sfibrils with an independent verification possible by used as both primary fixative an d embedding medium,freeze-fracture methodology (Leppard 1992a, b) . As is the internal details of cells were not well preserved.

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chemical characterization of aquatic submicron colloids.Xlikrochim Acta 117.39-47

Painter TJ (1983) Algal polysacch arides. In: Asplnall GO(ed) The polysaccharides. Academic Press, New York,p 195-285

Percival E, McDowell RH (1967 ) Chemistry an d enzym ol-ogy of marine algal polysaccharides. Academic Press,London

Perret D, Leppard GG, Miiller M , Belzile N , DeVitre R, BuffleJ (1991) Electron microscopy of aquatic colloids: non-pertu rbing prep aration of specimen s in the field. WatRes 25 1333-1343

Rees DA, Morris ER , Thom D, Madd en JK (1982) Shap esand interactions of carbohydrate chalns. In: Aspinall GO(ed) The polysaccharides. Academic Press, New York.p 195-290

Reynolds ES (1963) The use of lead citrate at high pH asThis article was submitted to the editor

an electron-opaque stain in electron microscopy. J CellBiol 17:208-212

Spurr AR (1969) A low-viscosity epoxy resin embedd~ngmedium for electron microscopy. J Ultrastruct Res 26:31-43

Strycek T. Acreman J, Kerry A, Leppard GG , Nermut bIV,Kushner DJ (1992) Extracellular fibril production byfreshwater algae and cyanobacteria. Microb Ecol 23.53-74

Suzuki N, Kato K (1953) Studies on suspended materials.Marine snow in th e sea. Part 1. Sources of ma rine snow .Bull Fac Fish Hokkaido Univ 4.132-135

van Boekel WHM (1992) Phaeocystjs colony mucus com-ponents and the importance of calcium ions for colonystability. Mar Ecol Prog Ser 8?:301-305

Whitfield C (1988) Bacterial extrace llular polysacc harides.Can J M~crobiol 4:415-420

Manuscript first received: Ju l y 6, 1995Revised version accepted: December l l , 1995