Length effects of cylindrical polymer brushes on their in vitro and … · Length effects of...
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Support Information for
Length effects of cylindrical polymer brushes on their in vitro and in vivo
properties
Zhengkui Zhanga, Ling’e Zhanga, Jiaojiao Zhaob, Cheng Lia, Wei Wu*a, Xiqun Jianga
aDepartment of Polymer Science & Engineering, College of Chemistry & Chemical Engineering, Nanjing University, Nanjing, 210023, People’s Republic of
China
bJiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, Jiangsu People’s
Republic of China
* To whom correspondence should be addressed
Email: [email protected]
Electronic Supplementary Material (ESI) for Biomaterials Science.This journal is © The Royal Society of Chemistry 2019
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Table of Contents
1. Supporting Figures S1-S10
2. Captions of Supporting Movie 1, Movie 2
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1. Supporting Figures S1-S10
Retention time (s)
250 300 350 400 450 500 550
Fig. S1. GPC curves of the PGMA for the syntheses of CPBs 1 (black), 2 (red) and 3 (blue).
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Fig. S2. 1H NMR spectra of PGMA in CDCl3 (top) and PGA in (CD3)2CO (bottom) for the
synthesis of CPBs 1.
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3500 3000 2500 2000 1500 1000
20
40
60
80
100
Tra
nsm
itta
nce (
%)
Wavenumber (cm-1)
2100 cm-1
Fig. S3. FT-IR spectrum of PGA for the synthesis of CPBs 1.
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a
b
Fig. S4. 1H NMR spectra of CPBs-Boc 1 (a) and CPBs 1 (b) in D2O.
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Fig. S5. 3D AFM images of CPBs 1 (a), 2 (b) and 3 (c), and cross-sections (red line) including
AFM height images of CPBs 1 (d), 2 (e) and 3 (f) adsorbed on mica from dilute water
solutions. Scale bars = 100 nm.
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012
.5 25 50 100
200
0
20
40
60
80
100
CPBs 1 CPBs 2 CPBs 3
a
Concentration(μg/ml)
Rela
tive c
ell v
iab
ilit
y(%
)
012
.5 25 50 100
200
0
20
40
60
80
100
CPBs 1 CPBs 2 CPBs 3
b
Concentration (μg/ml)
Rela
tive c
ell v
iab
ilit
y (
%)
0
12.5 25 50 10
020
0
0
20
40
60
80
100
CPBs 1 CPBs 2 CPBs 3
b
Concentration (μg/ml)
Rela
tive c
ell v
iab
ilit
y (
%)
Fig. S6. In vitro cytotoxicities of CPBs 1, 2 and 3 against SH-SY5Y (a) and HUVEC (b) cells
determined by MTT assay after 24 h incubation.
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Fig. S7. Permeabilities of CPBs 1 and CPBs 2 in MCs. Typical Z-stack images of SH-SY5Y MCs
coincubated with FITC-labeled CPBs 1 and RBITC-labeled CPBs 2 for 4 h acquired from the
center to the top of the spheroid in 20 μm intervals. Scale bars = 50 μm.
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Fig. S8. Permeabilities of CPBs 1 and CPBs 3 in MCs. Typical Z-stack images of SH-SY5Y MCs
coincubated with FITC-labeled CPBs 1 and RBITC-labeled CPBs 3 for 4 h acquired from the
center to the top of the spheroid in 20 μm intervals. Scale bars = 50 μm.
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0 2 4 6 8 10 120
10
20
30
40
50CPBs 1
CPBs 3
Time (h)
% ID
/mL
pla
sm
a
Fig. S9. Evolution with time of the concentrations of the FITC-labeled CPBs 1 and 3 in the
plasma of H22 tumor-bearing mice after tail-vein injection of the FITC-labeled CPBs 1 and
3, respectively.
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Fig. S10. (a) CLSM images of RAW264.7 cells after 4 h incubation with the FITC-labeled CPBs
1 (left) and 3 (right) at 37 oC, respectively. (b) MFI in RAW264.7 cells measured by flow
cytometry after 4 h incubation with the FITC-labeled CPBs 1 and 3 at 37 oC, respectively.
Data as mean values ± S.D. (n = 3).
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2. Captions of Supporting Movie 1, Movie 2
Supporting Movie 1. 360° rotational views of the 3D whole-body microPET images of a
subcutaneous H22 tumor-bearing mouse at different time points after tail-vein injection of
the 18F-labeled CPBs 1.
Supporting Movie 2. 360 rotational views of the 3D whole-body microPET images of a
subcutaneous H22 tumor-bearing mouse at different time points after tail-vein injection of
the 18F-labeled CPBs 3.