Len Men Ruou Nep Than
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Transcript of Len Men Ruou Nep Than
Food Fermentation:
Desirable product properties
Bioprocess by micro-organismsand their enzymes
Thực phẩm lên men
Bioprocess:
ingredients:food componentsmicro-organismswater
operations:physicalbiologicalthermal
ExteriorTextureOdourTaste
Desirable properties
Appeal
Volume reductionCooking time reductionShelf-lifeNutrient retentionValorisation of by-products
Desirable properties
Appeal
Utility
Desirable properties
Appeal
Utility
Functionality
SafetyDigestibilityProbiotic effectOther physiological effects
Research aspects
Microbiological composition and activity
Functionality Safety
Nutrition(digestibility, energy density,anti diarrhoea effects)
Volatile flavour
Products / models
Tropical origin
Locally available ingredients
Low-cost, simple fermentation techniques* traditional products* innovations based on traditional products
Tropical LAB fermented products
Ingredients Fermented product(Geography)
Mode ofconsumption
Milk:cow or buffalo dahi (India) yoghurt
Meat:pork or beef chorizo (Mexico) dry sausage
Fish:fish, salt,roasted rice
pla-ra (Thailand) fish paste
Tropical mould fermented products
Moulds Raw Materials Fermented foods
Amylomyces rouxii glutinous ricecassava
tape ketanpeuyeum
Aspergillus oryzae,A. soyae
soya + rice/barleysoya + wheat + salt
misosoya sauce
Penicilliumroqueforti
milk curd blue-veinedcheese
P. nalgiovense, P.chrysogenum
sausage meat mould-ripenedsausages
Rhizopusoligosporus, R.oryzae
soya beans tempe
Cases: LAB fermented cereal porridges
Mawè Process innovationmicrobiology starter development
Kenkey Process innovation
Complementary (weaning) foods
Safetynutrition
Collaboration:Bénin, Burkina Faso
Agbeli Mawe (Benin, West Africa)
1. Peeling 2. Washing 3. Grating 4. Dewatering 5. Sieving 6. Fine grinding
7. Kneading 8. Allowfermentation
9. Mixsuspension ofagbéli mawè
with hot water
10. Cook thepaste of agbéli
mawè
11. Bon appetit !
Agbeli Mawe
Meat Sauce
Gluey Sauce
Table 1. Changes in the microbial counts (log10 cfu/g wet wt) during fermentation of home-produced
mawè.
Fermentationtime (h)
pH Total aerobicmesophilicbacteria
Lactic acidbacteria
Lactobacilli Yeasts Enterobacteriaceae
0 6.25 6.5 6.5 6.3 4.4 2.56 4.35 9.1 9.2 9.0 4.8 3.812 4.02 9.1 9.2 9.1 4.9 3.224 3.85 9.3 9.2 9.3 6.5 3.448 3.75 9.0 9.1 9.1 7.3 < 1.772 3.65 9.0 9.2 9.1 7.5 < 1.7
Microbiology of mawè
Cases: Mould fermented soybeans
1. Tempe
Microbial ecologysafety microbiologynutrition
digestibilityanti diarrhoea
soybean
hydrated dehulled
moist cotyledons
cooked
drained, cooled and dried
inoculated with starter(Rhizopus oligosporus)
mixed
packed
incubated
FRESH TEMPE
2. Sufu
Process analysismycological aspectsprotein degradation studiesspoilage microflora, shelf-life studies
Cases: Mould fermented soybeans
Soybean Soaking Grinding Filtrate
Soymilk Coagulation Pressing Dicing
Tofu (Doufu ) Inoculation Fermentation (2~7d, 12~30°C)
Pehtze (Pizi ) Brine (3~7d) Maturation
Dressing mixtureSufu (Furu )
Component (g/100g Sufu) Component (mg/100g Sufu)
Moisture 58~70 Calcium 100~230
Crude protein 12~17 Phosphorus 150~300
Crude lipid 8~12 Iron 7~16
Crude fibre 0.2~1.5 Zinc 3~9
Carbohydrate 5~12 Niacin 0.5~1.2
Ash 4~9 Riboflavin 0.13~0.36
Food energy 460~750(KJ)
VB12 1.7~22
(ug)
Sufu
Cases: Yeast fermentations
Alcoholic fermentationAnimal feedingVolatile flavours
Purple glutinous rice
Starch-based starters
Crude turbid wine
Clear filtered wine
Purple glutinous rice
soak with waterfor 4 hoursat room temperature
steam at 100ºC for 1 hour
Apparatus to grind starter into powder
The gelatinized rice paste:cool to 40ºC inoculate with startermix well
Fungal growth under aerobic condition for 3 days at 30ºC
Alcoholic fermentation:under anaerobic conditionfor 7 daysat 30ºC
The whole fermented rice mass is homogenized
Clear wine
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