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Lecture 6: Molecular Techniques · 2007. 7. 31. · Lecture 6: Molecular Techniques 1. Restriction...
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1 Lecture 6: Molecular Techniques 1. Restriction Mapping 2. Molecular cloning Inserts and vectors Restriction enzymes and ligase Fig. 9., 9.5b, 9.6; 9.7, 9.8, 9.9, 9.10, 9.11, 9.12 Table 9.1, 9.2 Read p279-293
Transcript of Lecture 6: Molecular Techniques · 2007. 7. 31. · Lecture 6: Molecular Techniques 1. Restriction...
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Lecture 6: Molecular Techniques
1. Restriction Mapping2. Molecular cloning
Inserts and vectors
Restriction enzymes and ligaseFig. 9., 9.5b, 9.6; 9.7, 9.8, 9.9, 9.10,9.11, 9.12Table 9.1, 9.2Read p279-293
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Fig. 9.2
Fig. 9..2
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Fig. 9.6
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Fig. 9.5b
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Gel electrophoresis
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2. Molecular CloningInserts and vectors
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Restriction Digestion
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Fig. 9.7
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Fig. 9.8
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Fig. 9.9
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Genomic LibraryHuman: 3,000,000 kb/haploid genome150 kb per insert--20,000 clones equals a genome10kb per insert--300,000 clones (genome equivalent)
cDNA library
Expression Libraries
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Fig. 9.10
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Fig. 9.11