2012$%$BMMB$597D:$Analyzing$Next$Genera;on$Sequencing$Data$

$$Week$15,$Lecture$30$

István'Albert''

Biochemistry$and$Molecular$Biology$$and$Bioinforma;cs$Consul;ng$Center$

$Penn$State$

Discussion$on$the$project$

•  Some$observa;ons$on$the$project$

•  What$went$well$and$what$went$wrong$

•  Assembly$is$not$well$defined$%$small$changes$in$hash$sizes$!$different$results$

Assembly$on$forward$strand$

Assembly$on$reverse$strand$

Heng$Li’s$bioawk$

git$clone$hVps://github.com/lh3/bioawk.git$