Lecture 16: Plant hormone signal mechanisms 1. Ethylene 2...
Transcript of Lecture 16: Plant hormone signal mechanisms 1. Ethylene 2...
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Lecture 16: Plant hormone signal mechanisms
1. Ethylene2. Brassinosteroid
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Five classical plant hormones
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Brassinolide is a recently rediscovered hormone
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Fruit ripeningSenescence of leaves, flowersAbscission of leaves, flowers, fruitsAltered geotropism in roots and stemsPromotion of seed germinationInhibition/promotion of cell division and cell elongationInduction of phytoalexins and other disease resistancefactorsInitiation of rootsInhibition/promotion of floweringEpinasty of leavesBud dormancy releaseSex shifts in flowers
Responsesto Ethylene
C H2 4
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Ethylene Biosynthesis
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ethylene insensitive
Triple responses to ethylene
WT
-inhibition of stem elongation
-radial swelling of the stem
-apical hook formation
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etr1wt
ethylene air ethyleneairctr1wt ein2
etr1eto-1
Two types of mutants in ethylene mutants
Type 1: constitutive responseeto1 (ethylene over-producer)ctr1 (constitutive ethylene response)
Type 2: ethylene insensitiveetr1 (ethylene resistance)ein2, 3, 4,5 (ethylene insensitive)
eto1: phenotype can be blocked by ethylene synthesis inhibitorsctr1: phenotype is unaffected by ethylene synthesis inhibitors
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Ethylene CTR1 (Kinase)EIN2EIN3EIN5
triple response
ETR1
Epistasis pathway established by double mutant analyses
etr1 ctr1: constitutive responseein2 ctr1: ethylene insensitiveeto1 etr1: ethylene insensitiveeto1 ein2: ethylene insensitiveETO1
ctr1: constitutive responseseto1: constitutive responses:etr1: ethylene insensitiveein2: ethylene insensitive
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N CN CHSignalinput
domianhistidine kinase
Doutput signal
P
P
Sensor Response regulator
Two component system
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ERS1
ETR1
EIN4
ETR2
ERS2
53%
32%16-29%
58% 38%
52%
78%
44-54%
40%
64%
histidine kinase receiverGAFethylenebinding
38-41%
64%
54%
55%
Arabidopsis ethylene receptor family
Subfamily 1
Subfamily 2
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-etr1 is a dominant mutation-loss of etr and its redundant receptors gives rise to constitutive phenotypes
A. No ethylene
B. Yes ethylene
receptor CTR No ethylene response
receptor CTR Ethylene response
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CTR1(MAPKK?)
EIN3, EIL1, EIL2
ERF1 (other EREBPs?)
Ethylene-response gene induction
Ethylene
EIN2CN
Cytoplasm
Nucleus
?
(MAPK?)
TranscriptionFactors
ETR1 ERS1 ETR2 ERS2EIN4
D
NN
H)
D
Cu(I)
(H)
Cu(I)
(
NN
Cu(I) Cu(I)
(H) (H)
NN
Cu(I) Cu(I)
D
NN
D
Cu(I) Cu(I)
D
NN
H)
D
Cu(I)
(H)
Cu(I)
(H H H H
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YPD1
CTR1
Ethylene Responses
?
Ethylene
D
H
D
ERS1HH
ETR1
Arabidopsis
Osmolarity Responses
PBS2
HOG1
?
DSSK1
High Osmolarity
SLN1
Budding Yeast
DD
SSK2/SSK22
SHO1H H
?
H
H
?
MAPKKK
MAPKK
MAPK
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Figure 18.22, WT: wild-type. 14079 (TR): transgenic plant expressing ETR1-1
+ACC
after harvest
after harvest 16 hrs after ethylene treatment
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(A) BR biosynthetic mutantsDET2 encodes steroid 5a reductasedet2 is defective in biosynthesis of BRdet2 phenotype can be rescued by BR
Brassinosteroid (BR)
BR-defective mutants: dark green (light grown), small cells, reduced apical dominance.reduced male fertility. dark grown plants have similar phenotype to cop/det/fus, a role in photomorphogenesis
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kinase domain
transmembrane domain
leucine-rich repeat(LRR)
(I) BRI1 (BR receptor):Receptor like (serine/threonine) kinase (RLK)leucine-rich repeat (LRR) extracellular domainslocalizes to plasma membrane (thus differs from animals)
?
Plant cellAnimal cell
(B) BR signaling components
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(II) BIN2: member of a 10-member family glycogen synthase kinase
bin2: semidominant phenotype similar to bri (BR receptor mutation) negative regulator of BR signaling (III)BEZ1 and BZR1: highly similar in protein seq (89% identity)
6 member gene familynuclear protein (upon BR treatment)no DNA-binding domainnovel protein
Bez1 and bzr1: dominant mutant constitutive response phenotype
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A model of BR signaling pathway
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How do you distinguish mutants defective in BR synthesis (det2) from mutants defective in BR perception (bri) and signal transdection (bin2-D)?
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floral inductiongerminationhypocotyl elongationleaf expansionapical dominancefloral developmentfruit maturationinternode elongation
Gibberellic Acid
Lecture 17: Plant hormone signaling mechanism IIGA, Auxin, cytokinin, and ABA
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GA1: encodes for ent-CDP synthase
ga1-3: loss-of-function mutant of GA1
deficient for GA, do not germinate, short and dark green leavesdwarfreduced apical dominancecan be rescued by GA application
GA biosynthetic mutants
wt ga1-3
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gai: dominant allele mimics the effect of GA-deficiency harbors high levels of GA can’t be rescued by GA application, defective in GA signaling lose-of function allele tall plants insensitive to GA biosynthesis inhibitor paclobutrazol
(I) RGA, RGL1, GAI all encode GRAS (GAI RGA Scarecrow) transcription factor and serve as negative regulators of GA responses
GA signaling mutants
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Epistasis with GA biosynthetic mutants
GA1 RGAGAI
GA response
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RGA-GFP localization
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(II) SPINDLY (SPY)
Loss-of-SPY exhibits constitutive GA response
Encode OGT, which modifies other proteins in a manner similar tophosphorylation. Ie. SPY may add an O-GlcNAc moiety to other proteins to regulate their activities.
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GA deficient cell
GA functions as an inhibitor of inhibitors
SPYGAI
RGA
Tx of GA activated genes
SPY
GAIRGA
Tx of GA activated genes
WT cells
active form
Proposed GA receptor, not yet found
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Auxin: the growth hormone, required for viability
phototropismgravitropismgerminationdelay leaf abscissionpromote cell elongationpromote cell divisionpromote formation of lateral & adventitious rootsrequired for apical dominancevascular differentiation
Polar transport of auxin: synthesized in shoot tip
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ABP1: auxin binding protein 1 = Auxin receptor?
Auxin signaling mechanism
knockout mutants are lethal
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ARF: auxin-response factors 23 member family in Arabidopsis has DNA binding domains can be either activators or repressors
AUX/IAA: 29 member in Arabidopsis Has protein binding domain heterodimerize with ARFs rapid auxin-dependent turn-over of AUX/IAA is dependent on ubiquitin-proteasome-pathway
Genes identified in auxin signaling pathway
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Auxin signaling pathway
AUX/IAA ARFs
IAA IAA IAA
IAA
Degradation via SCFTIR1
Complex
Auxin-response geneAuxRE
TGTCTC 1. AUX/IAA2. SAUR3. GH34. GST (glutathion-S-transferase)5. ACC synthase
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ABA: Seed maturation and anti-stress signal
Initiation and maintenance of seed and bud dormancyacquire desiccation tolerance by inducing LEApromote seed storage proteins
prevent preharvest sprouting (vivipary)Response to stress (water stress)
close stomata in response to water stress
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No ABA receptor has been identified
Increased cytosolic Ca++ Ca++ influx through Plasma-membrane Ca++ release from vacuoles
Increase cellular PH (7.67 to 7.94)Membrane depolarization
In Stomatal guard cells:
ABA binding to receptor
ROS (Reactive Oxygen Species)
Stomata closing
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SCHEMATIC MODEL OF ABA Pathway
ABA
ABI1ABI2
P
STRESS RESPONSES (RD29A expression)
ABI1 and ABI2 encodephosphatase
Tanscription factor: ABI3, ABI4, and ABI5
IP3 & other second messengers
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Cytokinin-over producer:
Shoot apical meristem produce more leavesLeaves have higher chlorophyll levels & greenerAdventitious shoot may form from unwounded leafLeaf senescence is retardedAplical dominance is reducedRoot growth rate is reduced
Cytokinin: regulators of cell division
High auxin:cytokinin ratio: rootLow auxin/cytokinin ratio:shootIntermediate levels: callus
Morphogenesis in cultured tissues:
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Ti-plasmid
NOStmrtms
ori
vir
LB RB
LB, RB: 25 bp repeat, left and right bordertms: tumor morphology shoottmr: tumor morphology root ori: origin of replicationvir: virulence region
Agrobacteria causes crown-galltms: auxin synthesis (tms)tmr: cytokinin syntesis
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Receptor: CKI1: (over-producers that confer cytokinin-independent growth) CRE1: (mutants failed to produce shoots in response to cytokinin)AHK2AHK3
All encode two component signaling system
N CN CHSignalinput
domianhistidine kinase
D
output signal
P
P
Sensor Response regulator
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N CD
Type B ARRType A ARR
N D C
Receiver outputDomain domain (Tx factor)
ARR: Arabidopsis Response Regulator
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AHP: Arabidopsis Histidine Phosphotransfer
histidine kinase receiver AHP
P
D
ARR