Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas...
Transcript of Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas...
![Page 1: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/1.jpg)
Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors
Boris SZUREKMontpellier
France
GroupGTIPP,forGenomics&TranscriptomicsofPlant-ProcaryotesInterac>ons
![Page 2: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/2.jpg)
Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors
OutlineØ SomebackgroundaboutTALeffectors
Ø StoryI:searchforSWEET14loss-of-S
Ø StoryII:ablastNLRconfersRagainstXo
![Page 3: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/3.jpg)
Tomato
Arabidopsis
Cabbage
Rice Pepper
Cassava
Citrus
Plant pathogenic Xanthomonas
![Page 4: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/4.jpg)
TAL (Transcription Activator-Like) type III effectors
![Page 5: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/5.jpg)
TAL (Transcription Activator-Like) type III effectors
Ø They localize to the plant nucleus
Ø They induce expression of host genes
Ø How do they bind to DNA ?
TALsarebonafideplantTFs:
![Page 6: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/6.jpg)
•12+13:“hypervariable”
•4repeattypesinAvrBs3HD,NI,NG,NS
modifiedfrom:Schornacketal.(2006)J.PlantPhysiol.163:256.
TAL repeat types
![Page 7: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/7.jpg)
picture:AdamBogdanove
Bochetal.(2009)BreakingthecodeofDNAbindingspecificityofTAL-typeIIIeffectors.Science326:1509-1512;publishedonline29October2009(10.1126/science.1178811).
MoscouandBogdanove(2009)AsimpleciphergovernsDNArecogni>onbyTALeffectors.Science326:1501;publishedonline29October2009(10.1126/science.1178817).
The TAL code revealed
![Page 8: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/8.jpg)
Correlation between repeat types and UPA box
UPAboxconsensus
AvrBs3COOHNH2
17.5x34aa
LTPEQVVAIASHDGGKQALETVQRLLPVLCQAHGNINGNS
1 12/13 34
T A T A T A A A C C T N N C C C T C TDNA
HD NG NS NG NI NI NI HD HD NG NS NS HD HD HD NG HD NGRepeats
1 AvrBs3-repeat corresponds to 1 bp
![Page 9: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/9.jpg)
Different RVDs associate preferentially with different bases
Testthemodel!
![Page 10: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/10.jpg)
Target specificity of Hax2, Hax3, and Hax4
Bochetal.,Science,2009
![Page 11: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/11.jpg)
Artificial TAL effectors with novel specificities
Bochetal.,Science,2009
GUSgeneArtX-box
![Page 12: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/12.jpg)
GUSgeneArtX-box
Bochetal.,Science,2009
Artificial TAL effectors with novel specificities
![Page 13: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/13.jpg)
Crystal structure of a TAL bound to its DNA
Mak et al., Science, 2012
![Page 14: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/14.jpg)
![Page 15: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/15.jpg)
Onecanpredicttargets!
T
C
G
G
C
AT
C
A
T
C
TC
A
T
C
A
G
![Page 16: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/16.jpg)
Xanthomonasoryzaepv.oryzae(Xoo)
The Rice-Xoo interaction
![Page 17: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/17.jpg)
The Rice-Xoo interaction
![Page 18: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/18.jpg)
Sgene
TAL EBE
DISEASE
Roles of TAL effectors in compatible interactions
![Page 19: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/19.jpg)
5dpi1cm
5 kb
4 kb
2 kb
MAI1 BAI3
3 kb
talC
Yuetal.,MPMI,2011
TalC is a major virulence TAL effector
![Page 20: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/20.jpg)
5dpi1cm
5 kb
4 kb
2 kb
MAI1 BAI3
3 kb
talC
Yuetal.,MPMI,2011
SWEET14talC
TalCBAI3
TalC is a major virulence TAL effector
![Page 21: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/21.jpg)
SWEET/nodulin-3 proteins
. 22 members in rice
. Membrane Transporters
. Glucose & sucrose export
Talbot,2010,Nature
![Page 22: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/22.jpg)
Clade III SWEET genes confer TAL-ETS to Xoo
Streubel,Pesce,etal.,2013,NewPhytol.
![Page 23: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/23.jpg)
SWEET14talC
TalCBAI3
Burkina Faso
SWEET14 is targeted by multiple strains..
![Page 24: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/24.jpg)
SWEET14talC
TalCBAI3
tal5
Tal5MAI1
Burkina Faso
Mali
SWEET14 is targeted by multiple strains..
![Page 25: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/25.jpg)
pthXo3 SWEET14avrXa7 talC
TalCBAI3
PthXo3PXO61
tal5
Tal5MAI1
AvrXa7PXO86
Philippines Burkina Faso
…of different geographic origins and lineages
Mali
Susceptibility
SWEET14 is targeted by multiple strains..
![Page 26: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/26.jpg)
SWEET14
TalCBAI3
PthXo3PXO61
Tal5MAI1
AvrXa7PXO86
Philippines Burkina Faso
Mali
? ? ?
Loss of Susceptibility
![Page 27: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/27.jpg)
ü Unique collection of 132 wild and cultivated rice accessions
ü Sequencingofa400-bpPCRproduct
3
65
pthXo3 SWEET14avrXa7 talC tal5
30 34
Search for polymorphism in the SWEET14 promoter
![Page 28: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/28.jpg)
PATTERN1
PATTERN2
PATTERN3
AvrXa7 Tal5
Search for polymorphism in the SWEET14 promoter
![Page 29: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/29.jpg)
SWEET14avrXa7 talC tal5
Δ
Does it work ?
![Page 30: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/30.jpg)
Nodele>on
Δ18bp
5DPI
SWEET14avrXa7 talC tal5
Δ
Does it work ?
![Page 31: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/31.jpg)
Nodele>on
Δ18bp5DPI
SWEET14avrXa7 talC tal5
Δ
Does it work ?
![Page 32: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/32.jpg)
Nodele>on
Δ18bp2DPI
SWEET14avrXa7 talC tal5
Δ
Does it work ?
![Page 33: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/33.jpg)
• Reduced disease symptoms
• Impaired Xoo multiplication
• No induction of SWEET14
Ø The xa41(t) allele confers resistance by loss of susceptibility to Xoo strains relying on Tal5 and AvrXa7
Summary
![Page 34: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/34.jpg)
Asia
Africa
50% of Xoo strains are defeated by xa40(t)
![Page 35: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/35.jpg)
PATTERN1
PATTERN2
PATTERN3
AvrXa7 Tal5
What about pattern 3 ?
![Page 36: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/36.jpg)
PATTERN1
PATTERN2
PATTERN3
AvrXa7 Tal5
What about pattern 3 ?
![Page 37: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/37.jpg)
A portion of SWEET14 is duplicated in O. glaberrima..
![Page 38: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/38.jpg)
A duplication of pSWEET14 occurred in O. barthii ancestor
![Page 39: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/39.jpg)
A duplication of pSWEET14 occurred in O. barthii ancestor
Hu>netal.,2015,PlantJ
![Page 40: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/40.jpg)
Conclusions – Part I
Ø Based on the TAL code, an allele with an 18bp deletion was predicted to resist to Xoo strains relying on AvrXa7 and Tal5
Ø New HTP methodology for the isolation of loss-of-S resistance alleles
Ø Avoid laborious germplasm phenotyping
![Page 41: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/41.jpg)
Hu>netal.,Fron>ersinPlantScience,2015
![Page 42: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/42.jpg)
Ralf Koebnik Lionel Gagnevin Valérie Verdier
Sébastien Cunnac Servane Baufume Jonathan Jacobs Florence Auguy Mathilde Hutin Céline Pesce Alvaro Perez
Tu Tran Tuan Hinda Doucoure
Carolina Flores Cheick Tekete
Sandrine Fabre
Alain Ghesquière François Sabot
![Page 43: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/43.jpg)
NewFron>erProject«Menergep»(IRD,Cirad,JIRCAS&AfricaRice)
Funding
![Page 44: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/44.jpg)
A Rice Blast Resistance Gene Confers Resistance Against BLB and BLS
ThomasKrojStellaCesariVéroniqueChalvon
MathildeHu>nTuanTuTranRalfKoebnik
![Page 45: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/45.jpg)
Xoo - X.oryzaepv.oryzae
Xoc - X.oryzaepv.oryzicola
BLB
BLS
HydathodeWounds
StomateWounds
Vascular bundle
Mesophyll intercellular space
Xanthomonas oryzae pathovars profiles
![Page 46: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/46.jpg)
Xoo - X.oryzaepv.oryzae
Xoc - X.oryzaepv.oryzicola
BLB
BLS
HydathodeWounds
StomateWounds
Vascular bundle
Mesophyll intercellular space
Xanthomonas oryzae pathovars profiles
ClonedRgenes:Xa1Xa21Xa27Xa10Xa23
![Page 47: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/47.jpg)
Xoo - X.oryzaepv.oryzae
Xoc - X.oryzaepv.oryzicola
BLB
BLS
HydathodeWounds
StomateWounds
Vascular bundle
Mesophyll intercellular space
Xanthomonas oryzae pathovars profiles
ClonedRgenes:Xa1Xa21Xa27Xa10Xa23
NoRgeneavailable(inrice)
![Page 48: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/48.jpg)
Bogdanoveetal.,COPB,2010
TAL effectors during resistance and disease
![Page 49: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/49.jpg)
Bochetal.,NewPhytol,2014
Engineered resistance against TALEs
Bs3,Xa27,Xa10
![Page 50: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/50.jpg)
Bochetal.,NewPhytol,2014
Engineered resistance against TALEs
Bs3,Xa27,Xa10
![Page 51: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/51.jpg)
Bochetal.,NewPhytol,2014
Engineered resistance against TALEs
Bs3,Xa27,Xa10
![Page 52: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/52.jpg)
Ø Causal agent : Magnaporthe
oryzae
Ø Host : Oryza spp.
Ø Hemibiotrophic pathogen
Rice Blast
![Page 53: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/53.jpg)
Rice
M.oryzae
Pi-CO39 pi-CO39
Avr1-CO39
avr1-CO39
The Avr1-C039 / Pi-CO39 interaction
![Page 54: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/54.jpg)
Rice
M.oryzae
Pi-CO39 pi-CO39
Avr1-CO39
avr1-CO39
The Avr1-C039 / Pi-CO39 interaction
![Page 55: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/55.jpg)
Cesarietal.,PlantCell,2013Cesarietal.,EMBO,2014
Pi-CO39 resistance is mediated by a pair of NB-LRR proteins
![Page 56: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/56.jpg)
KitaakePi-CO39
Kanto51pi-CO39
AVR1-CO39UAS
AVR1-CO39UAS
uidAUAS
uidAUAS
DEX-inducible AVR1-CO39 transgenic lines
![Page 57: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/57.jpg)
Kitaake(Pi-CO39)
Kanto51(pi-co39)
pUAS
gal4::u
idA
pUAS
gal4::AV
R1-CO39
DEX
+
-
+
-
Line1 Line2
DEX-inducible AVR1-CO39 transgenic lines
![Page 58: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/58.jpg)
DEX-inducible AVR1-CO39 transgenic lines
![Page 59: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/59.jpg)
pINDE
X::AVR
1-CO
39:HA
pINDE
X::uidA
Kanto51(pi-co39)
DEX + - + - + -
Line1 Line2
Kitaake(Pi-CO39)
DEX-induction of AVR1-CO39 triggers cell death and ROS
![Page 60: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/60.jpg)
Kitaake(Pi-C
O39)
Kanto5
1(pi-co3
9)
+ - + -
pINDEX::AVR1-CO39 pINDEX::uidA
DEX
DEX-induction of AVR1-CO39 triggers cell death and ROS
![Page 61: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/61.jpg)
KitaakePi-CO39
Kanto51pi-CO39
AVR1-CO39
dTALEUAS
UAS
AVR1-CO39
dTALEUAS
UAS
uidA
dTALEUAS
UASuidA
dTALEUAS
UAS
Using dTALEs to induce AVR1-CO39
![Page 62: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/62.jpg)
0,00
0,05
0,10
0,15
0,20 mockEVdTALE
RelaNv
eAV
R1CO
39expression
∅ ∅∅
Kitaake(Pi-CO39)
Kanto51(pi-co39)
uidAAVR1-CO39Line1
AVR1-CO39Line2
AVR1-CO39
Using dTALEs to induce AVR1-CO39
![Page 63: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/63.jpg)
0,00
0,05
0,10
0,15
0,20 mockEVdTALE
RelaNv
eAV
R1CO
39expression
∅ ∅∅
Kitaake(Pi-CO39)
Kanto51(pi-co39)
uidAAVR1-CO39Line1
AVR1-CO39Line2
AVR1-CO39
TheAVR1-CO39anduidAgenesareinducedbyXanthomonasoryzaecarryingthedTALEUAS
Using dTALEs to induce AVR1-CO39
![Page 64: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/64.jpg)
The induction of AVR1-CO39 confers resistance to Xoo
![Page 65: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/65.jpg)
.. and Xoc
![Page 66: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/66.jpg)
The induction of AVR1-CO39 confers resistance to Xoo
0
5
10
15
20
25
30
35
Line1 Line2 Line1 Line2 Line1 Line1
pUASgal4::AVR1-CO39 pUASgal4::uidA pUASgal4::AVR1-CO39
pUASgal4::uidA
Kitaake Kanto51
lesion
length(cm)
PXO99EV
PXO99ArTAL
PXO99
PXO99(EV)
PXO99(dTALE)
PXO99 PXO99Kitaake(Pi-CO39) Kanto51(pi-CO39)
![Page 67: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/67.jpg)
The induction of AVR1-CO39 confers resistance to Xoo
0
5
10
15
20
25
30
35
Line1 Line2 Line1 Line2 Line1 Line1
pUASgal4::AVR1-CO39 pUASgal4::uidA pUASgal4::AVR1-CO39
pUASgal4::uidA
Kitaake Kanto51
lesion
length(cm)
PXO99EV
PXO99ArTAL
PXO99
PXO99(EV)
PXO99(dTALE)
PXO99 PXO99Kitaake(Pi-CO39) Kanto51(pi-CO39)
![Page 68: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/68.jpg)
The induction of AVR1-CO39 confers resistance to Xoo
0
1
2
3
4
5
6
7
8
9
Line1 Line2 Line1 Line2 Line1 Line1
pUASgal4::AVR1-CO39 pUASgal4::uidA pUASgal4::AVR1-CO39
pUASgal4::uidA
Kitaake Kanto51
Bacterialpop
ualNon
slog10(C
FU)
PXO99EV
PXO99ArTAL
PXO99PXO99(EV)
PXO99(dTALE)
PXO99 PXO99Kitaake(Pi-CO39) Kanto51(pi-CO39)2
1
![Page 69: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/69.jpg)
The induction of AVR1-CO39 confers resistance to Xoo
0
1
2
3
4
5
6
7
8
9
Line1 Line2 Line1 Line2 Line1 Line1
pUASgal4::AVR1-CO39 pUASgal4::uidA pUASgal4::AVR1-CO39
pUASgal4::uidA
Kitaake Kanto51
Bacterialpop
ualNon
slog10(C
FU)
PXO99EV
PXO99ArTAL
PXO99PXO99(EV)
PXO99(dTALE)
PXO99 PXO99Kitaake(Pi-CO39) Kanto51(pi-CO39)2
1
![Page 70: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/70.jpg)
Cesarietal.,PlantCell,2013Cesarietal.,EMBO,2014
Pi-CO39 resistance is mediated by a pair of NB-LRR proteins
![Page 71: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/71.jpg)
Induction of RGA4 in Pi-CO39 lines confers resistance to Xoo
RGA4
dTALERGA4
0
2
4
6
8
10
12
lesion
length(cm)
Co39
02468
101214161820
Kitaake
PXO99
Lesio
nlength(cm.)
Lesio
nlength(cm.)
PXO99
PXO99PXO99
PXO99
PXO99PXO99PXO99
PXO99Co39 Kitaake
0
5
10
15
20
25
Kanto51PXO99Kanto51
PXO99PXO99
PXO99
PXO99
Lesio
nlength(cm.)
Hu>netal.,PlantJ,2016
![Page 72: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/72.jpg)
Ø TheRGA4/RGA5-mediatedresistanceisabletocontrolBLBandBLS
Ø Autoac>veformsofNB-LRRsandAVRscanbeusedfor
synthe>cpromoter-trapsresistancestrategies
tal2 RGA4tal1
Tal1
Tal2
tal3
Tal3
Conclusions
![Page 73: Learning from the pathogen towards tailored-sustainable resistance : the case study of Xanthomonas TAL effectors](https://reader034.fdocuments.in/reader034/viewer/2022042906/58ae1ea71a28ab7e4a8b5697/html5/thumbnails/73.jpg)
ThomasKrojStellaCesariVéroniqueChalvon
MathildeHu>nTuanTuTranRalfKoebnik
Thanks !