Learn more about our qPCR kits
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Transcript of Learn more about our qPCR kits
![Page 1: Learn more about our qPCR kits](https://reader036.fdocuments.in/reader036/viewer/2022062320/55c5dcebbb61ebc93b8b4691/html5/thumbnails/1.jpg)
Real Time PCR assays for veterinary diagnosis
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Real Time PCR (qPCR) assays Use of qPCR assays for diagnosis of animal diseases have increased
considerably: Better sensitivity and specificity. Short time results. Capable of quantification.
Drawbacks of most commercial qPCR assays:
Several reagents: more variability inter-assay and possibility of mistakes.
Different designs: each qPCR has a different setup / thermal profile. Poor validation: “Research use only”
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Liquid format“OneMIX” Real Time PCR assays
Stabilized formatReal Time PCR assays
All qPCR reagents in a single tube. A novel technology allows stabilization of all reagents into a qPCR plate.
Pathogen and EC PRIMERS
Endogenous control’s
PROBE (HEX)
Pathogen’sPROBE (FAM)
Enzyme
BUFFERS & dNTPs
Shipment/Store at 4°C.
Shipment/Store at -20°C.
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Make qPCR easy
1. Select the required number of reactions.
2. Add 15µl nucleic acid free water/each well.
4. Run your qPCR.
Get results in less than 2 hours!
3. Add 5µl of DNA sample / PC/ NC.
2. Just add 15µl of oneMIX reaction in each tube/well.
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Other advantages:
Avoid false negative results: endogenous control (EC) in each well.
Pathogen detection (FAM)
EC detection (HEX)
Relative or absolute quatification: specific synthetic positive controls.
Same thermal protocol: test several pathogens in a single run.
Pathogen APathogen B
Pathogen C
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Multiparametric qPCR assays for Porcine abortion panel
PCV2
PPV
C. abortus
C. suis
Leptospira
SAMPLE
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Multiparametric qPCR assays for E. coli virulence factors
Edema disease(ED)
Post-weaning diarrhea(PWD)
Stx2e F18 F4 Sta Stb LT EC
SAMPLE
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Conveniently validated:
• Reference strain, vaccine strain, field strains.
• 42 other related pathogens.
Sensitivity and Specificity
• Reportanble range: using specific positive controls.
• Intra-assay e inter-assay studies.
Limits of detection (LOD)
Precision
• Concordance study: commercial qPCR assay or other diagnostics test.Accuracy
• Different species.• Several type of samples.Clinical cases
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Stabilized qPCR assays
PATHOGENS
1Bovine Herpesvirus
12 Mycoplasma bovis
3Pathogenic Leptospira
4 Toxoplasma gondii
5 Coxiella burnetii
6 Neospora caninum
7Tritrichomonas
foetus
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Available “oneMIX” qPCR assays
BESN EXOone Besnoitia besnoitiHPS
UEXOone Haemophilus parasuis
MHYR
EXOone Mycoplasma hyorhinisEXOone Salmonella
entericaSENT
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Available “oneMIX” qPCR assays
CRYP
EXOone Cryptosporidium spp
In testing phase:
BVDV/BDV
Bovine Respiratory Syncytial virus
Bovine Parainfluenza 3
RHDV new variant
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Thank you
for your
attention!