Laura Boykin - Opening Plenary

Laura M. Boykin1, Karen Armstrong1, Laura Kubatko2 and, Paul De Barro3

1Bio-Protection Research Centre, PO Box 84, Lincoln University, Lincoln 7647, New Zealand2 Departments of Statistics and Evolution, Ecology, and Organismal Biology, The Ohio State University,

Columbus, Ohio 43210, USA.3 CSIRO Ecosystem Sciences, GPO Box 2583, Brisbane QLD 4001

Bioprotection science for New Zealand

Species Delimitation and Global Biosecurity

Thank you:

Organizers:David Schindel et al.

Co-authors:Paul De BarroKaren ArmstrongLaura Kubatko

The Story:

MeLaura K.

Species Delimitationphylogeneticscoalescent theoryetc.

The Story:


Paul

B. tabaci/biosecurity

MeLaura K.

Bemisia tabaciCrop value loss = $500 million annually since 1991 due to B. tabaci (United States).

cassava sweetpotatotomato

cottonornamentals

melon crop devastated by B. tabaci

B. tabaci in action

-feeding damage-transmit viruses-insecticide resistant

2007 - Global Phylogeny:

2007 - Global Phylogeny:

“Genetic Groups”

2010- Global Phylogeny- Dinsdale et al.

% divergence....

2010. Annals of the Entomological Society of America, 103, 196–208.

3%-2

4 gr

oups

11%

-11

grou

ps

De Barro et al. 2011:

Genetic groups and associated biotype

determinations

The Story continued:


Paul Karen

Global Biosecuritytradeplant protectionetc.

MeLaura K.

Paul Karen

The Story: Current study

MeLaura K.


Global Biosecuritytradeplant protectionetc.

Intersection


New Study: The Intersection

• Integration of phylogenetic advances, specifically species delimitation measures, applied to the real-world


• Integration of phylogenetic advances, specifically species delimitation measures, applied to the real-world

• If we change the names i.e. elevate genetic groups to species level-- downstream issues exist.

- international trade regulations- local trade regulations- effectiveness of insecticides

Boykin, L.M., K. Armstrong, L. Kubatko, and P. De Barro. 2011. Species Delimitation and Global Biosecurity. Evolutionary Bioinformatics. In Press.

New Study: The Intersection

How many “species” are there?

0.57 0.99

1

1

0.84

0.72

1

0.62

0.32

0.67

1

0.17 0.17

0.81

1

1

1

1

0.43

0.98

1

0.88

0.43

0.69

0.85

0.09

0.94

0.98

1

1

1

0.86

1

0.09

0.63

1

0.03

0.1

1

0.78

0.53

0.54

1

1

0.39

0.95

1

0x100

0.71

1

1

0.1 0.1

0.58

0.53

1

0.02

0.09

1

1

1

0.98

0.16 0.17

0.43

0.75 0.34

0x100

0x100

0x100

0x100

0x100

0x100

0.02

0.71

0.33

0.85

0.18 0.98

0.2

0.01

0.07

0.98

1

0.68

0.17

0.87

1

0.99

0.43

0.85 0.83

0x100

0.02

0.02 0.02 0.02 0.02 0.03 0.02

0.04 0.02 0.01 0.02

0.72

1

0.98

1

0.93

1

0.21

0x100

0.51

0.09 0.07 0.9

1

1

0.79

0.3

0.02

1

1

0.34

0.99 1

0.02

0.02

0.88

0.97

0.69

0.21

0.09

0.95

1

0.16

0.36

0.3

1

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0.01

0.61

0.34

0.51

1

1 1

1

0x100

0.06

0.41

0.98

0.95

0.99

0.99

1

0.23

0.91

0.21

0.03

0.1

1

0.34

0.06

0.04

0.99

0.34

0x100

0.01

0.09

0.57

0.01

0.02

0.01

0.02 0.02

0.02 0.02 0.02 0.02 0.02

0.02 0.02 0.02 0.02

0.52

1

1

1

0.85

0x100

0x100

0.25

1 0.34

0.34

0.98

1

0.53

0.09 0.1

0.04

0.12 0.12

0.01

0.06

0.07

0.07

0.02 0.86

0.42 0.3 0.28

0.95 0.58 0.92

0.75 0.79

1

0.26

0.31

0.85

0.69

0.92

0.16

0.05

0.06 0.05

0.99

0.85

0.43 0.72

1

0x100

0.04

0.01

0.03 1

0.02

0.01

0.65

1

0.26

0.37

0.74

0.09

0.3

0.32

0.88

0.66

1

0x100

0x100

1

0.09 0.06 0.34

0.7 0.08 0.08 0.08

0.5

0.29

0.13

0.73

0.04

0.37

0.17 0.18

0.03

0.04

0x100

0x100

0.88

1

1

0.41

0.03

0.17 0.14

0.11 0.11

0.91 0.23

0.67

0.05

0.03

0.02

0.04

0.42

0.6

0.38

0.02

0.09

0.89

0.78

0.13

0.67

0.67 0.76 0.93

0.39

0.16

0.08 0.09

0.33

0x100

0.52

0.04

0.11

0.95

0.24

0.19

0.87

0x100

0x100

0.09 0.11 0.97

0.34

0.1

0.35

0x100

0.01

0x100

0.04 0.03

0.99

0.29

0.34 0.19

0.03

0.03

0.28 0.55

0.04 0.04

0.19

0.42

0.77

1

0.99

0.45

0.23 0.22

Bemisia_berbericola_new Bemisia_subdecipiens_GU220056

Bemisia_tuberculata_AY057220 Bemisia_afer_GQ139515 Bemisia_afer_GU220055

Bemisia_emiliae_DQ989555

Uganda_Namulonge_AY903576

Bemisia_atriplex2_GU086363 Uganda_Uganda_AF418665 Uganda_Namulonge_AY903579 Uganda_Namulonge_AY903577


Bemisia_atriplex_GU086362 Bemisia_on_Rhagodia_parabolica

NewWorld_Sudan_EU760727

SubSahAf3_Cameroon_AF344257

SubSahAf4_Cameroon_AF344247 SubSahAf4_Cameroon_AF344255

Italy_AY827598

NewWorld_Argentina_AF340212 NewWorld_Argentina_AF340213

SubSahAf1_H42_Uganda_AY057149 SubSahAf1_H43_Uganda_AY057151


MidEastAm2_Reunion_AJ550177

IndianOcean_Namulonge_AY903523 IndianOcean_Namulonge_AY903525

NewWorld_Colombia_AJ550167

SubSahAf1_H44_Uganda_AY057169

SubSahAf2_Mali_AY827605 SubSahAf2_SpainIpomea_GU086361 SubSahAf2_Spain_AY827611

SubSahAf4_Cameroon_AF344249 SubSahAf4_Cameroon_AF344254 SubSahAf4_Cameroon_AF344251

SubSahAf4_H36_Cameroon_AF344246

Mediterranean_Hap12_Burkina_Faso_FJ766405

MidEastAm1_China_Zhejiang_GQ332577

Indian_Ocean_MS_Madagascar_AJ550171 Indian_Ocean_MS_Reunion_AJ550178

AsiaII_8_India_AJ748362

Asia1_India_AJ748359

ex_China_EU192051

Italy_AY827596 Italy_AY827600

Italy_AY827601

NewWorld_USA_A_biotype_AY521259 NewWorld_Colombia_AJ550168

NewWorld_Colombia_EU427728

SubSahAf2_Namulonge_AY903493 SubSahAf2_Namulonge_AY903466

Mediterranean_Burkina_Faso_FJ766385 Mediterranean_Hap11_Burkina_Faso_FJ766387 Mediterranean_Burkina_Faso_FJ766408

IndianOcean_Masindi_AY903522

AsiaII_7_China_AY686064


Asia1_China_DQ986959

Australia_Bundaberg_GU086328

China2_AY686072

Italy_AY827603

NewWorld_Panama_DQ130060 NewWorld_Panama_DQ130061

NewWorld_Guatemala_AY057129 NewWorld_Honduras_AF342770

SubSahAf1_Uganda_AY057183 SubSahAf2_Uganda_Masaka_AY563648


SubSahAf2_Mukono_AY903480

SubSahAf2_Namulonge_AY903507 SubSahAf2_Namulonge_AY903492 SubSahAf2_Uganda_Kalangala_AY563702

SubSahAf1_H46_Uganda_AY057180 SubSahAf2_Namulonge_AY903490 SubSahAf2_Namulonge_AY903502 SubSahAf2_Masaka_AY903462

SubSahAf2_Masaka_AY903461 SubSahAf2_Namulonge_AY903470

SubSahAf2_Namulonge_AY903464 SubSahAf2_Mukono_AY903479

SubSahAf2_Namulonge_AY903513 SubSahAf2_Uganda_Mbarara_AY563695 SubSahAf2_Namulonge_AY903494 SubSahAf2_Uganda_Kalangala_AY563666

SubSahAf1_SouthAfrica_AF344264

SubSahAf2_Namulonge_AY903510

SubSahAf2_Mali_AY827604 SubSahAf2_Nigeria_AY827607


MidEastAm1_Hap6_China_Shandong_EF398087

Indian_Ocean_MS_Reunion_AJ550179 IndianOcean_Busukuma_AY903536

IndianOcean_Busukuma_AY903538 IndianOcean_Busukuma_AY903539

Indian_Ocean_MS_Reunion_AJ550180

AsiaII_7_China_AY686075 AsiaII_8_India_AJ748374


Australia_Indonesia_GU086326

China1_AY686091


NewWorld_Honduras_AY057133 NewWorld_BioSida_PuertoRico_AY057134

NewWorld_Belize_DQ130053



SubSahAf1_Uganda_AY057210

SubSahAf2_Uganda_Mbarara_AY563674 SubSahAf2_Uganda_Soroti_AY563679

SubSahAf2_Busukuma_AY903487

SubSahAf2_Uganda_Kabarole_Rural_AY563664 SubSahAf2_Uganda_Kibaale_AY563667

Mediterranean_Syria_Hama_AB297898

Mediterranean_AF344258_Cameroon

Mediterranean_Cote_dIvoire_FJ766383

Mediterranean_FJ766431_Burkino_Faso

MidEastAm1_Taiwan_Taidung_DQ174530 MidEastAm1_China_Jiangsu_EU263625

MidEastAm1_China_Shandong_EF398083

IndianOcean_Busukuma_AY903537 IndianOcean_Namulonge_AY903527



AsiaII_6_China_AJ784261 AsiaII_5_India_AJ748376

AsiaII_5_India_AF418666


Asia1_India_AJ748371 Asia1_India_AJ748361

Australia_Indonesia

NewWorld_Mexico_DQ130059

SubSahAf2_Uganda_Mbarara_AY563652 SubSahAf1_Uganda_AY057168



SubSahAf1_Swaziland_AF344276 SubSahAf1_Ghana_AY827591

SubSahAf1_H37_Ghana_AF418668 SubSahAf2_Namulonge_AY903511 SubSahAf2_Busukuma_AY903483

SubSahAf2_Uganda_AF418669 SubSahAf2_Uganda_AY057194

Mediterranean_EF667476_China_Hubei Mediterranean_Croatia_II_a_GU086334

Mediterranean_Croatia_II_b_GU086335

Mediterranean_Hap15_SubSahAfSL_Nigeria_AY827606 Mediterranean_Burkina_Faso_FJ766384

Mediterranean_Burkina_Faso_FJ766429

MidEastAm1_India_Karnataka_Bangalore_AJ748368

MidEastAm1_China_Yunnan_AY686078

MidEastAm1_China_Guangdong_DQ923043

MidEastAm1_Japan_Kumamoto_AB204580 MidEastAm1_USA_Arizona_AY057123

MidEastAm1_Japan_Hiroshima_DQ174537 MidEastAm1_Hap5_Japan_Kagoshima_AF340215

MidEastAm1_Cyprus_Parekklisia_AM945180 MidEastAm1_AY903542_Namulonge MidEastAm1_USA_Arizona MidEastAm1_Emerald_Australia MidEastAm1_Spain_Almeria_GU086348

MidEastAm1_Japan_Kochi_AB204578 MidEastAm1_Indonesia_Bogor MidEastAm1_Japan_Kumamoto_AB204581

MidEastAm1_Morocco_Saidia_AJ517768 MidEastAm1_Mayotte_AJ550173 MidEastAm1_Reunion_AJ550174 MidEastAm1_Guadeloupe_SaintFrancois_AM180064 MidEastAm1_Israel_DQ174536 MidEastAm1_Morocco_Nador_AM176570 MidEastAm1_Taiwan_Matsu_DQ174534 MidEastAm1_China_Fujian_AY686062 MidEastAm1_China_Shandong_EF398113


AsiaII_3_China_EU192045 AsiaII_6_China_EU192052 AsiaII_6_China_EU192054

AsiaII_7_DQ116660

Australia_Indonesia_GU086325 Australia_Indonesia_GU086327

China1_AY686085 China1_AY686089

China_1_GQ139495 China_1_GQ303180

NewWorld_Mexico_EU427729

SubSahAf2_Uganda_Iganga_AY563641 SubSahAf2_Uganda_Soroti_AY563657


SubSahAf2_Uganda_AY057173 SubSahAf2_Uganda_AY057141

SubSahAf2_Uganda_Kumi_AY563646

Mediterranean_Egypt_GU086331 Mediterranean_Hap23_Syria_Hama_AB297897

Mediterranean_Hap3_Turkey_AY827617 Mediterranean_Turkey_AY827619

Mediterranean_SubSahAfSL_Ghana_AY827587

MidEastAm1_Saudi_Arabdia_GU086344 MidEastAm1_Israel_AY766373

MidEastAm1_USA_Florida_GU086340 MidEastAm1_Australia_Bundaberg_DQ174535

MidEastAm1_China_Shanghai_DQ989525

MidEastAm1_Guadeloupe_SaintFrancois_AJ877260 MidEastAm1_China_Shandong_EF398091

MidEastAm1_Taiwan_GU086342

MidEastAm1_China_Jiangxi_AY686073 MidEastAm1_Hap7_Israel_Tel_Aviv_AF418671 MidEastAm1_Jordan_GU086347

MidEastAm1_Italy_Sicily_GU086341 MidEastAm1_DominicanRepublic_GU086349

MidEastAm1_Hap1_USA_Arizona_GU086340 MidEastAm1_China_Hainan_EU192068

MidEastAm1_India_Bangalore_AM408896 MidEastAm1_Israel_EF398127

MidEastAm1_China_Jiangsu_EU263624

MidEastAm1_Hap8_SouthKorea_Goyang_DQ174538 MidEastAm1_SouthKorea_Goyang_DQ989531

MidEastAm1_China_Shandong_EF398088 MidEastAm1_China_Shandong_EF398090

AsiaII_1_Pakistan_AY686094 AsiaII_1_Zee_PKPun16

AsiaII_3_China_ZHJ1_DQ309075


Asia1_Malaysia_AY686093 Asia1_Thailand_AF164671 Asia1_India_AJ748360 Asia1_India_AJ748369 Asia1_India_AJ748365 Asia1_India_AJ748370

NewWorld_ElSalvador_AY057128


SubSahAf1_Malawi_AY057162 SubSahAf1_Tanzania_AF418667

SubSahAf1_Mozambique_AF344278 SubSahAf1_SouthAfrica_AF344267


Mediterranean_Morocco_AM176573

Mediterranean_SubSahAfSL_Ghana_AY827589 Mediterranean_AY903534_Uganda

Mediterranean_Hap22_Uganda_AY903540

MidEastAm1_Israel_AY747688 MidEastAm1_Israel_new

MidEastAm1_India_Karnataka_Kolar_AM040594 MidEastAm1_Hap4_China_Guangzhou_AY686063

MidEastAm1_Taiwan_Hualien_DQ989539 MidEastAm1_Taiwan_Yilan_DQ989540


AsiaII_3_China_AJ783706 AsiaII_3_China_AJ867556

AsiaII_7_India_AJ748372 AsiaII_7_DQ174523

Asia1_Indonesia_AB248262

NewWorld_Mexico_AY057126 NewWorld_Mexico_DQ130058

Mediterranean_Taiwan_AOP_GU086339 Mediterranean_GU086339_Taiwan_AOP

Mediterranean_SubSahAfSL_Ghana_AY827579 Mediterranean_Hap9_Burkina_Faso_FJ766391


Mediterranean_Hap6_SubSahAfSL_IvoryCoast_AY057136

Mediterranean_AF344285_Zimbabwe Mediterranean_AF344286_Zimbabwe

Mediterranean_AY903529_Namulonge Mediterranean_AY903551_Kabarole Mediterranean_AY903531_Namulonge Mediterranean_AY903556_Mukono Mediterranean_AY903564_Namulonge

Mediterranean_AY903565_Namulonge

MidEastAm1_Hap2_Pakistan_Sindh_AJ510074

AsiaII_1_Zee_1PKPun7 AsiaII_1_Zee_PKPun18

Asia1_Indonesia_AB248260 Asia1_Singapore_AY686095 Asia1_China_EU192044 Asia1_Thailand_EF528552

Mediterranean_GQ139500_China_Zhejiang Mediterranean_Hap14_Egypt_FJ025797 Mediterranean_GU168793_China_Fuzhou

Mediterranean_SubSahAfSL_Ghana_AY827580 Mediterranean_AY827581_Ghana

Mediterranean_SubSahAfSL_Ghana_AY827588 Mediterranean_SubSahAfSL_Ghana_AY827590

MidEastAm1_Iraq_Al_A_Zamiyah

AsiaII_1_Pakistan_AJ510057 AsiaII_1_Zee_PKPun13 AsiaII_1_Zee_Syria_S3

AsiaII_1_China_AJ867557 AsiaII_1_China_EU192047

AsiaII_1_China_EU192065 AsiaII_1_Pakistan_AJ510065

Asia1_Pakistan_AJ510078 Asia1_Pakistan_AJ510061 Asia1_Zee_PKPun3

Mediterranean_Spain_AF342769

Mediterranean_Spain_DQ174539 Mediterranean_Hap1_China_Q_GU086329 Mediterranean_EF694111_China_Hubei

Mediterranean_AM176572_Morocco

MidEastAm1_Saudi_Arabia_GU086345 MidEastAm1_Kuwait_GU086354 MidEastAm1_Kuwait_GU086355

MidEastAm1_United_Arab_Emirates_DQ133382

MidEastAm1_Hap9_Pakistan_Sindh_AJ510073 MidEastAm1_Zee_PKSin12

AsiaII_1_Zee_PKPun14

Asia1_Pakistan_AJ510066 Asia1_Pakistan_AJ510069

Mediterranean_Hap20_Cameroon_EU760721 Mediterranean_Algeria_AM176574

Mediterranean_Algeria_AM176575 Mediterranean_China_Hubei_EF694112

Mediterranean_EF694108_Egypt_Zawiat_Razin

MidEastAm1_Iran_GU086351

MidEastAm1_Iran_EU547770

MidEastAm1_Hap10_Pakistan_Sindh_AJ510076 MidEastAm1_Hap2_Syria_AlHasakeh_AB473559 MidEastAm1_Iran_EU547772

AsiaII_1_Pakistan_AJ510058 AsiaII_1_Zee_Syria_S7

Mediterranean_France_AM691055 Mediterranean_Sudan_AY827612

Mediterranean_Hap8_Sudan1_DQ133378

Mediterranean_Hap4_Czech_B1_GU086330

MidEastAm1_Pakistan_Sindh_AJ510075 MidEastAm1_Pakistan_Sindh_AJ510079

MidEastAm1_Hap3_Pakistan_Sindh_AJ510071 MidEastAm1_Hap3_Pakistan_Sindh_AJ510081

Mediterranean_Hap18_Crete_DQ365856 Mediterranean_Burkina_Faso_FJ766381 Mediterranean_Uganda_AY903578

Mediterranean_Sudan_AY827615 Mediterranean_Cameroon

Mediterranean_Hap21_France_AM691062 Mediterranean_Hap16_Crete_DQ365857

MidEastAm1_Kuwait_GU086346

Mediterranean_Hap7_China2_Beijing_GU086332 Mediterranean_EU760760_Uruguay

Mediterranean_Hap17_Spain_AF342775 Mediterranean_Morocco_AM176571

Mediterranean_Hap5_Burkina_Faso_FJ766432 Mediterranean_AM040609_Ghana


MidEastAm1_Yemen_GU086360 MidEastAm1_Iran_GU086350 MidEastAm1_Yemen_GU086359

Mediterranean_Hap13_Morocco_AF342773

Mediterranean_DQ302946_Spain_Almeria

Mediterranean_Croatia_I_c_GU086337 Mediterranean_Sudan_AY827613 Mediterranean_Sudan_AY827614

Mediterranean_France_AM691063 Mediterranean_Hap2_China_4_GU086333

Mediterranean_Morocco_AJ517769 Mediterranean_Spain_DQ174540

MidEastAm1_Saudi_Aradia_GU086358

MidEastAm1_Iran_GU086352 MidEastAm1_Kuwait_GU086356

Mediterranean_France_AM180063 Mediterranean_China_Yunnan_EU192061 Mediterranean_EU192049_China_Yunnan

Mediterranean_Croatia_II_d_GU086336 Mediterranean_Croatia_Split_a_GU086338

MidEastAm1_Yemen_GU086343 MidEastAm1_Saudi_Aradia_GU086357

Mediterranean_Morocco_AY057138 Mediterranean_Hap19_France_AM691059 Mediterranean_France_AM691064

1.0

Our Approach:1. Predefined groups: 2. Tip to root

Species Delimitation Measures (K2P/P(AB)/P(RD)/PP/gsi/GMYC)


partition the tree tip to root

Assessment of measures to indicate distinctiveness


0.57 0.99

1

1

0.84

0.72

1

0.62

0.32

0.67

1

0.17 0.17

0.81

1

1

1

1

0.43

0.98

1

0.88

0.43

0.69

0.85

0.09

0.94

0.98

1

1

1

0.86

1

0.09

0.63

1

0.03

0.1

1

0.78

0.53

0.54

1

1

0.39

0.95

1

0x100

0.71

1

1

0.1 0.1

0.58

0.53

1

0.02

0.09

1

1

1

0.98

0.16 0.17

0.43

0.75 0.34

0x100

0x100

0x100

0x100

0x100

0x100

0.02

0.71

0.33

0.85

0.18 0.98

0.2

0.01

0.07

0.98

1

0.68

0.17

0.87

1

0.99

0.43

0.85 0.83

0x100

0.02

0.02 0.02 0.02 0.02 0.03 0.02

0.04 0.02 0.01 0.02

0.72

1

0.98

1

0.93

1

0.21

0x100

0.51

0.09 0.07 0.9

1

1

0.79

0.3

0.02

1

1

0.34

0.99 1

0.02

0.02

0.88

0.97

0.69

0.21

0.09

0.95

1

0.16

0.36

0.3

1

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0.01

0.61

0.34

0.51

1

1 1

1

0x100

0.06

0.41

0.98

0.95

0.99

0.99

1

0.23

0.91

0.21

0.03

0.1

1

0.34

0.06

0.04

0.99

0.34

0x100

0.01

0.09

0.57

0.01

0.02

0.01

0.02 0.02

0.02 0.02 0.02 0.02 0.02

0.02 0.02 0.02 0.02

0.52

1

1

1

0.85

0x100

0x100

0.25

1 0.34

0.34

0.98

1

0.53

0.09 0.1

0.04

0.12 0.12

0.01

0.06

0.07

0.07

0.02 0.86

0.42 0.3 0.28

0.95 0.58 0.92

0.75 0.79

1

0.26

0.31

0.85

0.69

0.92

0.16

0.05

0.06 0.05

0.99

0.85

0.43 0.72

1

0x100

0.04

0.01

0.03 1

0.02

0.01

0.65

1

0.26

0.37

0.74

0.09

0.3

0.32

0.88

0.66

1

0x100

0x100

1

0.09 0.06 0.34

0.7 0.08 0.08 0.08

0.5

0.29

0.13

0.73

0.04

0.37

0.17 0.18

0.03

0.04

0x100

0x100

0.88

1

1

0.41

0.03

0.17 0.14

0.11 0.11

0.91 0.23

0.67

0.05

0.03

0.02

0.04

0.42

0.6

0.38

0.02

0.09

0.89

0.78

0.13

0.67

0.67 0.76 0.93

0.39

0.16

0.08 0.09

0.33

0x100

0.52

0.04

0.11

0.95

0.24

0.19

0.87

0x100

0x100

0.09 0.11 0.97

0.34

0.1

0.35

0x100

0.01

0x100

0.04 0.03

0.99

0.29

0.34 0.19

0.03

0.03

0.28 0.55

0.04 0.04

0.19

0.42

0.77

1

0.99

0.45

0.23 0.22











Italy_AY827598
















ex_China_EU192051


Italy_AY827601










China2_AY686072

Italy_AY827603






















China1_AY686091























Australia_Indonesia






















AsiaII_7_DQ116660






































































































1.0







1 species13+ biotypes24 genetic groups

Species Delimitation Stats

• 2007- Rosenberg’s P(AB)

• 2008- Rodrigo et al.’s P(RD)

Masters, B. C., V. Fan, and H. A. Ross. 2010. Species delimitation – a Geneious plugin for the exploration of species boundaries. Molecular Ecology Resources 11:154-7.

Cummings, M. P. et al. 2008. A genealogical approach to quantifying lineage divergence. Evolution 62:2411-22.

Pons, J., et. al. 2006. Sequence-based species delimitation for the DNA taxonomy of undescribed insects. Systematic Biology 55: 595-609.

• 2008- Cummings et al.’s gsi• 2009- Monaghan et al.’s GMYC

Results: Predefined groups

0.57 0.99

1

1

0.84

0.72

1

0.62

0.32

0.67

1

0.17 0.17

0.81

1

1

1

1

0.43

0.98

1

0.88

0.43

0.69

0.85

0.09

0.94

0.98

1

1

1

0.86

1

0.09

0.63

1

0.03

0.1

1

0.78

0.53

0.54

1

1

0.39

0.95

1

0x100

0.71

1

1

0.1 0.1

0.58

0.53

1

0.02

0.09

1

1

1

0.98

0.16 0.17

0.43

0.75 0.34

0x100

0x100

0x100

0x100

0x100

0x100

0.02

0.71

0.33

0.85

0.18 0.98

0.2

0.01

0.07

0.98

1

0.68

0.17

0.87

1

0.99

0.43

0.85 0.83

0x100

0.02

0.02 0.02 0.02 0.02 0.03 0.02

0.04 0.02 0.01 0.02

0.72

1

0.98

1

0.93

1

0.21

0x100

0.51

0.09 0.07 0.9

1

1

0.79

0.3

0.02

1

1

0.34

0.99 1

0.02

0.02

0.88

0.97

0.69

0.21

0.09

0.95

1

0.16

0.36

0.3

1

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0.01

0.61

0.34

0.51

1

1 1

1

0x100

0.06

0.41

0.98

0.95

0.99

0.99

1

0.23

0.91

0.21

0.03

0.1

1

0.34

0.06

0.04

0.99

0.34

0x100

0.01

0.09

0.57

0.01

0.02

0.01

0.02 0.02

0.02 0.02 0.02 0.02 0.02

0.02 0.02 0.02 0.02

0.52

1

1

1

0.85

0x100

0x100

0.25

1 0.34

0.34

0.98

1

0.53

0.09 0.1

0.04

0.12 0.12

0.01

0.06

0.07

0.07

0.02 0.86

0.42 0.3 0.28

0.95 0.58 0.92

0.75 0.79

1

0.26

0.31

0.85

0.69

0.92

0.16

0.05

0.06 0.05

0.99

0.85

0.43 0.72

1

0x100

0.04

0.01

0.03 1

0.02

0.01

0.65

1

0.26

0.37

0.74

0.09

0.3

0.32

0.88

0.66

1

0x100

0x100

1

0.09 0.06 0.34

0.7 0.08 0.08 0.08

0.5

0.29

0.13

0.73

0.04

0.37

0.17 0.18

0.03

0.04

0x100

0x100

0.88

1

1

0.41

0.03

0.17 0.14

0.11 0.11

0.91 0.23

0.67

0.05

0.03

0.02

0.04

0.42

0.6

0.38

0.02

0.09

0.89

0.78

0.13

0.67

0.67 0.76 0.93

0.39

0.16

0.08 0.09

0.33

0x100

0.52

0.04

0.11

0.95

0.24

0.19

0.87

0x100

0x100

0.09 0.11 0.97

0.34

0.1

0.35

0x100

0.01

0x100

0.04 0.03

0.99

0.29

0.34 0.19

0.03

0.03

0.28 0.55

0.04 0.04

0.19

0.42

0.77

1

0.99

0.45

0.23 0.22











Italy_AY827598
















ex_China_EU192051


Italy_AY827601










China2_AY686072

Italy_AY827603






















China1_AY686091























Australia_Indonesia






















AsiaII_7_DQ116660






































































































1.0

= 5 out of 6 stats are “+” for taxonomic distinctiveness

SubSaharan Africa

Mediterranean (Q)

Middle East Asia Minor 1 (B)

+/+/+/+/+/+

+/+/+/+/+/+

+/+/+/-/+/+Species Delimitation Measures: K2PP(AB)P(RD)PPgsiGMYC

0.57 0.99

1

1

0.84

0.72

1

0.62

0.32

0.67

1

0.17 0.17

0.81

1

1

1

1

0.43

0.98

1

0.88

0.43

0.69

0.85

0.09

0.94

0.98

1

1

1

0.86

1

0.09

0.63

1

0.03

0.1

1

0.78

0.53

0.54

1

1

0.39

0.95

1

0x100

0.71

1

1

0.1 0.1

0.58

0.53

1

0.02

0.09

1

1

1

0.98

0.16 0.17

0.43

0.75 0.34

0x100

0x100

0x100

0x100

0x100

0x100

0.02

0.71

0.33

0.85

0.18 0.98

0.2

0.01

0.07

0.98

1

0.68

0.17

0.87

1

0.99

0.43

0.85 0.83

0x100

0.02

0.02 0.02 0.02 0.02 0.03 0.02

0.04 0.02 0.01 0.02

0.72

1

0.98

1

0.93

1

0.21

0x100

0.51

0.09 0.07 0.9

1

1

0.79

0.3

0.02

1

1

0.34

0.99 1

0.02

0.02

0.88

0.97

0.69

0.21

0.09

0.95

1

0.16

0.36

0.3

1

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0.01

0.61

0.34

0.51

1

1 1

1

0x100

0.06

0.41

0.98

0.95

0.99

0.99

1

0.23

0.91

0.21

0.03

0.1

1

0.34

0.06

0.04

0.99

0.34

0x100

0.01

0.09

0.57

0.01

0.02

0.01

0.02 0.02

0.02 0.02 0.02 0.02 0.02

0.02 0.02 0.02 0.02

0.52

1

1

1

0.85

0x100

0x100

0.25

1 0.34

0.34

0.98

1

0.53

0.09 0.1

0.04

0.12 0.12

0.01

0.06

0.07

0.07

0.02 0.86

0.42 0.3 0.28

0.95 0.58 0.92

0.75 0.79

1

0.26

0.31

0.85

0.69

0.92

0.16

0.05

0.06 0.05

0.99

0.85

0.43 0.72

1

0x100

0.04

0.01

0.03 1

0.02

0.01

0.65

1

0.26

0.37

0.74

0.09

0.3

0.32

0.88

0.66

1

0x100

0x100

1

0.09 0.06 0.34

0.7 0.08 0.08 0.08

0.5

0.29

0.13

0.73

0.04

0.37

0.17 0.18

0.03

0.04

0x100

0x100

0.88

1

1

0.41

0.03

0.17 0.14

0.11 0.11

0.91 0.23

0.67

0.05

0.03

0.02

0.04

0.42

0.6

0.38

0.02

0.09

0.89

0.78

0.13

0.67

0.67 0.76 0.93

0.39

0.16

0.08 0.09

0.33

0x100

0.52

0.04

0.11

0.95

0.24

0.19

0.87

0x100

0x100

0.09 0.11 0.97

0.34

0.1

0.35

0x100

0.01

0x100

0.04 0.03

0.99

0.29

0.34 0.19

0.03

0.03

0.28 0.55

0.04 0.04

0.19

0.42

0.77

1

0.99

0.45

0.23 0.22











Italy_AY827598
















ex_China_EU192051


Italy_AY827601










China2_AY686072

Italy_AY827603






















China1_AY686091























Australia_Indonesia






















AsiaII_7_DQ116660






































































































1.0

Species Delimitation Stats

• Let’s utilize species delimitation stats and remove subjectivity surrounding species/biotype/genetic group designations.

“Tip to Root” Approach:

?

?

?

?

?

?

0.57 0.99

1

1

0.84

0.72

1

0.62

0.32

0.67

1

0.17 0.17

0.81

1

1

1

1

0.43

0.98

1

0.88

0.43

0.69

0.85

0.09

0.94

0.98

1

1

1

0.86

1

0.09

0.63

1

0.03

0.1

1

0.78

0.53

0.54

1

1

0.39

0.95

1

0x100

0.71

1

1

0.1 0.1

0.58

0.53

1

0.02

0.09

1

1

1

0.98

0.16 0.17

0.43

0.75 0.34

0x100

0x100

0x100

0x100

0x100

0x100

0.02

0.71

0.33

0.85

0.18 0.98

0.2

0.01

0.07

0.98

1

0.68

0.17

0.87

1

0.99

0.43

0.85 0.83

0x100

0.02

0.02 0.02 0.02 0.02 0.03 0.02

0.04 0.02 0.01 0.02

0.72

1

0.98

1

0.93

1

0.21

0x100

0.51

0.09 0.07 0.9

1

1

0.79

0.3

0.02

1

1

0.34

0.99 1

0.02

0.02

0.88

0.97

0.69

0.21

0.09

0.95

1

0.16

0.36

0.3

1

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0.01

0.61

0.34

0.51

1

1 1

1

0x100

0.06

0.41

0.98

0.95

0.99

0.99

1

0.23

0.91

0.21

0.03

0.1

1

0.34

0.06

0.04

0.99

0.34

0x100

0.01

0.09

0.57

0.01

0.02

0.01

0.02 0.02

0.02 0.02 0.02 0.02 0.02

0.02 0.02 0.02 0.02

0.52

1

1

1

0.85

0x100

0x100

0.25

1 0.34

0.34

0.98

1

0.53

0.09 0.1

0.04

0.12 0.12

0.01

0.06

0.07

0.07

0.02 0.86

0.42 0.3 0.28

0.95 0.58 0.92

0.75 0.79

1

0.26

0.31

0.85

0.69

0.92

0.16

0.05

0.06 0.05

0.99

0.85

0.43 0.72

1

0x100

0.04

0.01

0.03 1

0.02

0.01

0.65

1

0.26

0.37

0.74

0.09

0.3

0.32

0.88

0.66

1

0x100

0x100

1

0.09 0.06 0.34

0.7 0.08 0.08 0.08

0.5

0.29

0.13

0.73

0.04

0.37

0.17 0.18

0.03

0.04

0x100

0x100

0.88

1

1

0.41

0.03

0.17 0.14

0.11 0.11

0.91 0.23

0.67

0.05

0.03

0.02

0.04

0.42

0.6

0.38

0.02

0.09

0.89

0.78

0.13

0.67

0.67 0.76 0.93

0.39

0.16

0.08 0.09

0.33

0x100

0.52

0.04

0.11

0.95

0.24

0.19

0.87

0x100

0x100

0.09 0.11 0.97

0.34

0.1

0.35

0x100

0.01

0x100

0.04 0.03

0.99

0.29

0.34 0.19

0.03

0.03

0.28 0.55

0.04 0.04

0.19

0.42

0.77

1

0.99

0.45

0.23 0.22











Italy_AY827598
















ex_China_EU192051


Italy_AY827601










China2_AY686072

Italy_AY827603






















China1_AY686091























Australia_Indonesia






















AsiaII_7_DQ116660






































































































1.0







Tip to Root: partition the tree

B. tabaci 0.57

0.99

1

1

0.84

0.72

1

0.62

0.32

0.67

1

0.17 0.17

0.81

1

1

1

1

0.43

0.98

1

0.88

0.43

0.69

0.85

0.09

0.94

0.98

1

1

1

0.86

1

0.09

0.63

1

0.03

0.1

1

0.78

0.53

0.54

1

1

0.39

0.95

1

0x100

0.71

1

1

0.1 0.1

0.58

0.53

1

0.02

0.09

1

1

1

0.98

0.16 0.17

0.43

0.75 0.34

0x100

0x100

0x100

0x100

0x100

0x100

0.02

0.71

0.33

0.85

0.18 0.98

0.2

0.01

0.07

0.98

1

0.68

0.17

0.87

1

0.99

0.43

0.85 0.83

0x100

0.02

0.02 0.02 0.02 0.02 0.03 0.02

0.04 0.02 0.01 0.02

0.72

1

0.98

1

0.93

1

0.21

0x100

0.51

0.09 0.07 0.9

1

1

0.79

0.3

0.02

1

1

0.34

0.99 1

0.02

0.02

0.88

0.97

0.69

0.21

0.09

0.95

1

0.16

0.36

0.3

1

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0.01

0.61

0.34

0.51

1

1 1

1

0x100

0.06

0.41

0.98

0.95

0.99

0.99

1

0.23

0.91

0.21

0.03

0.1

1

0.34

0.06

0.04

0.99

0.34

0x100

0.01

0.09

0.57

0.01

0.02

0.01

0.02 0.02

0.02 0.02 0.02 0.02 0.02

0.02 0.02 0.02 0.02

0.52

1

1

1

0.85

0x100

0x100

0.25

1 0.34

0.34

0.98

1

0.53

0.09 0.1

0.04

0.12 0.12

0.01

0.06

0.07

0.07

0.02 0.86

0.42 0.3 0.28

0.95 0.58 0.92

0.75 0.79

1

0.26

0.31

0.85

0.69

0.92

0.16

0.05

0.06 0.05

0.99

0.85

0.43 0.72

1

0x100

0.04

0.01

0.03 1

0.02

0.01

0.65

1

0.26

0.37

0.74

0.09

0.3

0.32

0.88

0.66

1

0x100

0x100

1

0.09 0.06 0.34

0.7 0.08 0.08 0.08

0.5

0.29

0.13

0.73

0.04

0.37

0.17 0.18

0.03

0.04

0x100

0x100

0.88

1

1

0.41

0.03

0.17 0.14

0.11 0.11

0.91 0.23

0.67

0.05

0.03

0.02

0.04

0.42

0.6

0.38

0.02

0.09

0.89

0.78

0.13

0.67

0.67 0.76 0.93

0.39

0.16

0.08 0.09

0.33

0x100

0.52

0.04

0.11

0.95

0.24

0.19

0.87

0x100

0x100

0.09 0.11 0.97

0.34

0.1

0.35

0x100

0.01

0x100

0.04 0.03

0.99

0.29

0.34 0.19

0.03

0.03

0.28 0.55

0.04 0.04

0.19

0.42

0.77

1

0.99

0.45

0.23 0.22











Italy_AY827598
















ex_China_EU192051


Italy_AY827601










China2_AY686072

Italy_AY827603






















China1_AY686091























Australia_Indonesia






















AsiaII_7_DQ116660






































































































1.0

B. tabaci 0.57

0.99

1

1

0.84

0.72

1

0.62

0.32

0.67

1

0.17 0.17

0.81

1

1

1

1

0.43

0.98

1

0.88

0.43

0.69

0.85

0.09

0.94

0.98

1

1

1

0.86

1

0.09

0.63

1

0.03

0.1

1

0.78

0.53

0.54

1

1

0.39

0.95

1

0x100

0.71

1

1

0.1 0.1

0.58

0.53

1

0.02

0.09

1

1

1

0.98

0.16 0.17

0.43

0.75 0.34

0x100

0x100

0x100

0x100

0x100

0x100

0.02

0.71

0.33

0.85

0.18 0.98

0.2

0.01

0.07

0.98

1

0.68

0.17

0.87

1

0.99

0.43

0.85 0.83

0x100

0.02

0.02 0.02 0.02 0.02 0.03 0.02

0.04 0.02 0.01 0.02

0.72

1

0.98

1

0.93

1

0.21

0x100

0.51

0.09 0.07 0.9

1

1

0.79

0.3

0.02

1

1

0.34

0.99 1

0.02

0.02

0.88

0.97

0.69

0.21

0.09

0.95

1

0.16

0.36

0.3

1

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0.01

0.61

0.34

0.51

1

1 1

1

0x100

0.06

0.41

0.98

0.95

0.99

0.99

1

0.23

0.91

0.21

0.03

0.1

1

0.34

0.06

0.04

0.99

0.34

0x100

0.01

0.09

0.57

0.01

0.02

0.01

0.02 0.02

0.02 0.02 0.02 0.02 0.02

0.02 0.02 0.02 0.02

0.52

1

1

1

0.85

0x100

0x100

0.25

1 0.34

0.34

0.98

1

0.53

0.09 0.1

0.04

0.12 0.12

0.01

0.06

0.07

0.07

0.02 0.86

0.42 0.3 0.28

0.95 0.58 0.92

0.75 0.79

1

0.26

0.31

0.85

0.69

0.92

0.16

0.05

0.06 0.05

0.99

0.85

0.43 0.72

1

0x100

0.04

0.01

0.03 1

0.02

0.01

0.65

1

0.26

0.37

0.74

0.09

0.3

0.32

0.88

0.66

1

0x100

0x100

1

0.09 0.06 0.34

0.7 0.08 0.08 0.08

0.5

0.29

0.13

0.73

0.04

0.37

0.17 0.18

0.03

0.04

0x100

0x100

0.88

1

1

0.41

0.03

0.17 0.14

0.11 0.11

0.91 0.23

0.67

0.05

0.03

0.02

0.04

0.42

0.6

0.38

0.02

0.09

0.89

0.78

0.13

0.67

0.67 0.76 0.93

0.39

0.16

0.08 0.09

0.33

0x100

0.52

0.04

0.11

0.95

0.24

0.19

0.87

0x100

0x100

0.09 0.11 0.97

0.34

0.1

0.35

0x100

0.01

0x100

0.04 0.03

0.99

0.29

0.34 0.19

0.03

0.03

0.28 0.55

0.04 0.04

0.19

0.42

0.77

1

0.99

0.45

0.23 0.22











Italy_AY827598
















ex_China_EU192051


Italy_AY827601










China2_AY686072

Italy_AY827603






















China1_AY686091























Australia_Indonesia






















AsiaII_7_DQ116660






































































































1.0

Tip to Root: partition the tree

0.57 0.99

1

1

0.84

0.72

1

0.62

0.32

0.67

1

0.17 0.17

0.81

1

1

1

1

0.43

0.98

1

0.88

0.43

0.69

0.85

0.09

0.94

0.98

1

1

1

0.86

1

0.09

0.63

1

0.03

0.1

1

0.78

0.53

0.54

1

1

0.39

0.95

1

0x100

0.71

1

1

0.1 0.1

0.58

0.53

1

0.02

0.09

1

1

1

0.98

0.16 0.17

0.43

0.75 0.34

0x100

0x100

0x100

0x100

0x100

0x100

0.02

0.71

0.33

0.85

0.18 0.98

0.2

0.01

0.07

0.98

1

0.68

0.17

0.87

1

0.99

0.43

0.85 0.83

0x100

0.02

0.02 0.02 0.02 0.02 0.03 0.02

0.04 0.02 0.01 0.02

0.72

1

0.98

1

0.93

1

0.21

0x100

0.51

0.09 0.07 0.9

1

1

0.79

0.3

0.02

1

1

0.34

0.99 1

0.02

0.02

0.88

0.97

0.69

0.21

0.09

0.95

1

0.16

0.36

0.3

1

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0.01

0.61

0.34

0.51

1

1 1

1

0x100

0.06

0.41

0.98

0.95

0.99

0.99

1

0.23

0.91

0.21

0.03

0.1

1

0.34

0.06

0.04

0.99

0.34

0x100

0.01

0.09

0.57

0.01

0.02

0.01

0.02 0.02

0.02 0.02 0.02 0.02 0.02

0.02 0.02 0.02 0.02

0.52

1

1

1

0.85

0x100

0x100

0.25

1 0.34

0.34

0.98

1

0.53

0.09 0.1

0.04

0.12 0.12

0.01

0.06

0.07

0.07

0.02 0.86

0.42 0.3 0.28

0.95 0.58 0.92

0.75 0.79

1

0.26

0.31

0.85

0.69

0.92

0.16

0.05

0.06 0.05

0.99

0.85

0.43 0.72

1

0x100

0.04

0.01

0.03 1

0.02

0.01

0.65

1

0.26

0.37

0.74

0.09

0.3

0.32

0.88

0.66

1

0x100

0x100

1

0.09 0.06 0.34

0.7 0.08 0.08 0.08

0.5

0.29

0.13

0.73

0.04

0.37

0.17 0.18

0.03

0.04

0x100

0x100

0.88

1

1

0.41

0.03

0.17 0.14

0.11 0.11

0.91 0.23

0.67

0.05

0.03

0.02

0.04

0.42

0.6

0.38

0.02

0.09

0.89

0.78

0.13

0.67

0.67 0.76 0.93

0.39

0.16

0.08 0.09

0.33

0x100

0.52

0.04

0.11

0.95

0.24

0.19

0.87

0x100

0x100

0.09 0.11 0.97

0.34

0.1

0.35

0x100

0.01

0x100

0.04 0.03

0.99

0.29

0.34 0.19

0.03

0.03

0.28 0.55

0.04 0.04

0.19

0.42

0.77

1

0.99

0.45

0.23 0.22











Italy_AY827598
















ex_China_EU192051


Italy_AY827601










China2_AY686072

Italy_AY827603






















China1_AY686091























Australia_Indonesia






















AsiaII_7_DQ116660






































































































1.0

= > 3.5% difference (+ 5 out of 6)

= new group (5 out of 6)

SubSaharan Africa

Middle East Asia Minor 1

Mediterranean


0.57 0.99

1

1

0.84

0.72

1

0.62

0.32

0.67

1

0.17 0.17

0.81

1

1

1

1

0.43

0.98

1

0.88

0.43

0.69

0.85

0.09

0.94

0.98

1

1

1

0.86

1

0.09

0.63

1

0.03

0.1

1

0.78

0.53

0.54

1

1

0.39

0.95

1

0x100

0.71

1

1

0.1 0.1

0.58

0.53

1

0.02

0.09

1

1

1

0.98

0.16 0.17

0.43

0.75 0.34

0x100

0x100

0x100

0x100

0x100

0x100

0.02

0.71

0.33

0.85

0.18 0.98

0.2

0.01

0.07

0.98

1

0.68

0.17

0.87

1

0.99

0.43

0.85 0.83

0x100

0.02

0.02 0.02 0.02 0.02 0.03 0.02

0.04 0.02 0.01 0.02

0.72

1

0.98

1

0.93

1

0.21

0x100

0.51

0.09 0.07 0.9

1

1

0.79

0.3

0.02

1

1

0.34

0.99 1

0.02

0.02

0.88

0.97

0.69

0.21

0.09

0.95

1

0.16

0.36

0.3

1

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0.01

0.61

0.34

0.51

1

1 1

1

0x100

0.06

0.41

0.98

0.95

0.99

0.99

1

0.23

0.91

0.21

0.03

0.1

1

0.34

0.06

0.04

0.99

0.34

0x100

0.01

0.09

0.57

0.01

0.02

0.01

0.02 0.02

0.02 0.02 0.02 0.02 0.02

0.02 0.02 0.02 0.02

0.52

1

1

1

0.85

0x100

0x100

0.25

1 0.34

0.34

0.98

1

0.53

0.09 0.1

0.04

0.12 0.12

0.01

0.06

0.07

0.07

0.02 0.86

0.42 0.3 0.28

0.95 0.58 0.92

0.75 0.79

1

0.26

0.31

0.85

0.69

0.92

0.16

0.05

0.06 0.05

0.99

0.85

0.43 0.72

1

0x100

0.04

0.01

0.03 1

0.02

0.01

0.65

1

0.26

0.37

0.74

0.09

0.3

0.32

0.88

0.66

1

0x100

0x100

1

0.09 0.06 0.34

0.7 0.08 0.08 0.08

0.5

0.29

0.13

0.73

0.04

0.37

0.17 0.18

0.03

0.04

0x100

0x100

0.88

1

1

0.41

0.03

0.17 0.14

0.11 0.11

0.91 0.23

0.67

0.05

0.03

0.02

0.04

0.42

0.6

0.38

0.02

0.09

0.89

0.78

0.13

0.67

0.67 0.76 0.93

0.39

0.16

0.08 0.09

0.33

0x100

0.52

0.04

0.11

0.95

0.24

0.19

0.87

0x100

0x100

0.09 0.11 0.97

0.34

0.1

0.35

0x100

0.01

0x100

0.04 0.03

0.99

0.29

0.34 0.19

0.03

0.03

0.28 0.55

0.04 0.04

0.19

0.42

0.77

1

0.99

0.45

0.23 0.22











Italy_AY827598
















ex_China_EU192051


Italy_AY827601










China2_AY686072

Italy_AY827603






















China1_AY686091























Australia_Indonesia






















AsiaII_7_DQ116660






































































































1.0 0.57

0.99

1

1

0.84

0.72

1

0.62

0.32

0.67

1

0.17 0.17

0.81

1

1

1

1

0.43

0.98

1

0.88

0.43

0.69

0.85

0.09

0.94

0.98

1

1

1

0.86

1

0.09

0.63

1

0.03

0.1

1

0.78

0.53

0.54

1

1

0.39

0.95

1

0x100

0.71

1

1

0.1 0.1

0.58

0.53

1

0.02

0.09

1

1

1

0.98

0.16 0.17

0.43

0.75 0.34

0x100

0x100

0x100

0x100

0x100

0x100

0.02

0.71

0.33

0.85

0.18 0.98

0.2

0.01

0.07

0.98

1

0.68

0.17

0.87

1

0.99

0.43

0.85 0.83

0x100

0.02

0.02 0.02 0.02 0.02 0.03 0.02

0.04 0.02 0.01 0.02

0.72

1

0.98

1

0.93

1

0.21

0x100

0.51

0.09 0.07 0.9

1

1

0.79

0.3

0.02

1

1

0.34

0.99 1

0.02

0.02

0.88

0.97

0.69

0.21

0.09

0.95

1

0.16

0.36

0.3

1

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0.01

0.61

0.34

0.51

1

1 1

1

0x100

0.06

0.41

0.98

0.95

0.99

0.99

1

0.23

0.91

0.21

0.03

0.1

1

0.34

0.06

0.04

0.99

0.34

0x100

0.01

0.09

0.57

0.01

0.02

0.01

0.02 0.02

0.02 0.02 0.02 0.02 0.02

0.02 0.02 0.02 0.02

0.52

1

1

1

0.85

0x100

0x100

0.25

1 0.34

0.34

0.98

1

0.53

0.09 0.1

0.04

0.12 0.12

0.01

0.06

0.07

0.07

0.02 0.86

0.42 0.3 0.28

0.95 0.58 0.92

0.75 0.79

1

0.26

0.31

0.85

0.69

0.92

0.16

0.05

0.06 0.05

0.99

0.85

0.43 0.72

1

0x100

0.04

0.01

0.03 1

0.02

0.01

0.65

1

0.26

0.37

0.74

0.09

0.3

0.32

0.88

0.66

1

0x100

0x100

1

0.09 0.06 0.34

0.7 0.08 0.08 0.08

0.5

0.29

0.13

0.73

0.04

0.37

0.17 0.18

0.03

0.04

0x100

0x100

0.88

1

1

0.41

0.03

0.17 0.14

0.11 0.11

0.91 0.23

0.67

0.05

0.03

0.02

0.04

0.42

0.6

0.38

0.02

0.09

0.89

0.78

0.13

0.67

0.67 0.76 0.93

0.39

0.16

0.08 0.09

0.33

0x100

0.52

0.04

0.11

0.95

0.24

0.19

0.87

0x100

0x100

0.09 0.11 0.97

0.34

0.1

0.35

0x100

0.01

0x100

0.04 0.03

0.99

0.29

0.34 0.19

0.03

0.03

0.28 0.55

0.04 0.04

0.19

0.42

0.77

1

0.99

0.45

0.23 0.22











Italy_AY827598
















ex_China_EU192051


Italy_AY827601










China2_AY686072

Italy_AY827603






















China1_AY686091























Australia_Indonesia






















AsiaII_7_DQ116660






































































































1.0


Current “species” line

= > 3.5 % difference

= new group

= old species line

• Bemisia tabaci: – trade: Support for 29 additional clades.

• 3 within clades 8 and 9 (highly invasive individuals in these clade). Revision of international and local trade policies pending taxonomic revision.

– chemical companies: testing against new species is expensive.

Implications:

Karen

Implications:Testing “tip to root”

L. dispar/biosecurity

• Not a well-resolved phylogeny• Subspecies recognized• Females-winged (Asia) or wingless (Europe/

USA)

Lymantria dispar

Different strains/subspecies have different invasive capabili4es

Second Example: Lymantriadefoliation of forests

pheromone trap eggs

Results: tip to root

L. dispar

= new group

= predefined group

• Lymantria dispar: – taxonomy- re-evaluation of characteristics that

might impact on trapping and surveillance [species specific pheromone lures]

– invasive capability: lacking statistical and phylogenetic support for diagnosis [winged vs. wingless]

Implications:

Methodology Summary: • “Tip to root” approach removes subjectivity out of

species delimitation. • Provide a blueprint for future studies.

• K2P, PP, P(AB) and P(RD) are integrated in Geneious and USER FRIENDLY, gsi and GMYC more difficult.

• All techniques described here can be applied to YOUR phylogeny.

Take Home Message:

Species Delimitation

Programmers-Make user friendly

Global Biosecurity

Regulators-Use the statistics

Intersection

Take Home Message:

Species Delimitation Global Biosecurity

Intersection

Blueprints

0.57 0.99

1

1

0.84

0.72

1

0.62

0.32

0.67

1

0.17 0.17

0.81

1

1

1

1

0.43

0.98

1

0.88

0.43

0.69

0.85

0.09

0.94

0.98

1

1

1

0.86

1

0.09

0.63

1

0.03

0.1

1

0.78

0.53

0.54

1

1

0.39

0.95

1

0x100

0.71

1

1

0.1 0.1

0.58

0.53

1

0.02

0.09

1

1

1

0.98

0.16 0.17

0.43

0.75 0.34

0x100

0x100

0x100

0x100

0x100

0x100

0.02

0.71

0.33

0.85

0.18 0.98

0.2

0.01

0.07

0.98

1

0.68

0.17

0.87

1

0.99

0.43

0.85 0.83

0x100

0.02

0.02 0.02 0.02 0.02 0.03 0.02

0.04 0.02 0.01 0.02

0.72

1

0.98

1

0.93

1

0.21

0x100

0.51

0.09 0.07 0.9

1

1

0.79

0.3

0.02

1

1

0.34

0.99 1

0.02

0.02

0.88

0.97

0.69

0.21

0.09

0.95

1

0.16

0.36

0.3

1

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0.01

0.61

0.34

0.51

1

1 1

1

0x100

0.06

0.41

0.98

0.95

0.99

0.99

1

0.23

0.91

0.21

0.03

0.1

1

0.34

0.06

0.04

0.99

0.34

0x100

0.01

0.09

0.57

0.01

0.02

0.01

0.02 0.02

0.02 0.02 0.02 0.02 0.02

0.02 0.02 0.02 0.02

0.52

1

1

1

0.85

0x100

0x100

0.25

1 0.34

0.34

0.98

1

0.53

0.09 0.1

0.04

0.12 0.12

0.01

0.06

0.07

0.07

0.02 0.86

0.42 0.3 0.28

0.95 0.58 0.92

0.75 0.79

1

0.26

0.31

0.85

0.69

0.92

0.16

0.05

0.06 0.05

0.99

0.85

0.43 0.72

1

0x100

0.04

0.01

0.03 1

0.02

0.01

0.65

1

0.26

0.37

0.74

0.09

0.3

0.32

0.88

0.66

1

0x100

0x100

1

0.09 0.06 0.34

0.7 0.08 0.08 0.08

0.5

0.29

0.13

0.73

0.04

0.37

0.17 0.18

0.03

0.04

0x100

0x100

0.88

1

1

0.41

0.03

0.17 0.14

0.11 0.11

0.91 0.23

0.67

0.05

0.03

0.02

0.04

0.42

0.6

0.38

0.02

0.09

0.89

0.78

0.13

0.67

0.67 0.76 0.93

0.39

0.16

0.08 0.09

0.33

0x100

0.52

0.04

0.11

0.95

0.24

0.19

0.87

0x100

0x100

0.09 0.11 0.97

0.34

0.1

0.35

0x100

0.01

0x100

0.04 0.03

0.99

0.29

0.34 0.19

0.03

0.03

0.28 0.55

0.04 0.04

0.19

0.42

0.77

1

0.99

0.45

0.23 0.22











Italy_AY827598
















ex_China_EU192051


Italy_AY827601










China2_AY686072

Italy_AY827603






















China1_AY686091























Australia_Indonesia






















AsiaII_7_DQ116660






































































































1.0

0.57 0.99

1

1

0.84

0.72

1

0.62

0.32

0.67

1

0.17 0.17

0.81

1

1

1

1

0.43

0.98

1

0.88

0.43

0.69

0.85

0.09

0.94

0.98

1

1

1

0.86

1

0.09

0.63

1

0.03 0.1

1

0.78

0.53

0.54

1

1

0.39

0.95

1

0x100

0.71

1

1

0.1 0.1

0.58

0.53

1

0.02

0.09

1

1

1

0.98

0.16 0.17

0.43

0.75 0.34

0x100

0x100

0x100

0x100

0x100

0x100

0.02

0.71

0.33

0.85

0.18 0.98

0.2

0.01

0.07

0.98

1

0.68

0.17

0.87

1

0.99

0.43

0.85 0.83

0x100

0.02

0.02 0.02 0.02 0.02 0.03 0.02

0.04 0.02 0.01 0.02

0.72

1

0.98

1

0.93

1

0.21

0x100

0.51

0.09 0.07 0.9

1

1

0.79

0.3

0.02

1

1

0.34

0.99 1

0.02

0.02

0.88

0.97

0.69

0.21

0.09

0.95

1

0.16

0.36

0.3

1

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0x100

0.01

0.61

0.34

0.51

1

1 1

1

0x100

0.06

0.41

0.98

0.95

0.99

0.99

1

0.23

0.91

0.21

0.03

0.1

1

0.34

0.06

0.04

0.99

0.34

0x100

0.01

0.09

0.57

0.01

0.02

0.01

0.02 0.02

0.02 0.02 0.02 0.02 0.02

0.02 0.02 0.02 0.02

0.52

1

1

1

0.85

0x100

0x100

0.25

1 0.34

0.34

0.98

1

0.53

0.09 0.1

0.04

0.12 0.12

0.01

0.06

0.07

0.07

0.02 0.86

0.42 0.3 0.28

0.95 0.58 0.92

0.75 0.79

1

0.26

0.31

0.85

0.69

0.92

0.16

0.05

0.06 0.05

0.99

0.85

0.43 0.72

1

0x100

0.04

0.01

0.03 1

0.02

0.01

0.65

1

0.26

0.37

0.74

0.09

0.3

0.32

0.88

0.66

1

0x100

0x100

1

0.09 0.06 0.34

0.7 0.08 0.08 0.08

0.5

0.29

0.13

0.73

0.04

0.37

0.17 0.18

0.03

0.04

0x100

0x100

0.88

1

1

0.41

0.03

0.17 0.14

0.11 0.11

0.91 0.23

0.67

0.05

0.03

0.02

0.04

0.42

0.6

0.38

0.02

0.09

0.89

0.78

0.13

0.67

0.67 0.76 0.93

0.39

0.16

0.08 0.09

0.33

0x100

0.52

0.04

0.11

0.95

0.24

0.19

0.87

0x100

0x100

0.09 0.11 0.97

0.34

0.1

0.35

0x100

0.01

0x100

0.04 0.03

0.99

0.29

0.34 0.19

0.03

0.03

0.28 0.55

0.04 0.04

0.19

0.42

0.77

1

0.99

0.45

0.23 0.22











Italy_AY827598
















ex_China_EU192051


Italy_AY827601










China2_AY686072

Italy_AY827603






















China1_AY686091























Australia_Indonesia






















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1.0

Take Home Message:

Blueprints

insecticide resistancemating

behaviortaxonomy

virus transmission

endosymbiont community

morphologypheromone lures

biological controlshost plant usage

more genes

Bio-Protection Research CentrePO Box 84Lincoln UniversityLincoln 7647, New ZealandP + 64 3 325 3696 F + 64 3 325 3864 www.bioprotection.org.nz


Acknowledgments:Co-authorsOrganizers

TEC FundingMAFBNZ

Vladimir Mencl Bluefern supercomputer facilities

University of Canterbury

Jeremy deWaardLymantria data

Rupert CollinsLincoln University

GMYC help

Thank you for your attention

Bio-Protection Research CentrePO Box 84Lincoln UniversityLincoln 7647, New ZealandP + 64 3 325 3696 F + 64 3 325 3864 www.bioprotection.org.nz


Thank you for your attention

Laura Boykin - Opening Plenary

Education

Transcript of Laura Boykin - Opening Plenary