Landscape level detection, characterization and genomic...
Transcript of Landscape level detection, characterization and genomic...
Landscape level detection, characterization
and genomic study of Newcastle Disease and
Avian Influenza
Poultry Conference 2018
November 19-20
Kathmandu, NEPAL
Diagnostic
Assay
Vaccine
Development
& Stabilization
Landscape
Disease Study
Infectious Disease Dynamics and
Landscape Epidemiology Research
needed for the front end of vaccine
development and efficacy testing,
including disease burden analysis on
landscape level using GIS and modeling.
Serological, Immunological and Molecular based
disease detection and diagnosis. We also specialize
in genomics based multiple pathogen detection and
characterization.
Outbreak and routine pathogen screening services
for rapid assessment and containment strategies.
Vaccine target assessment, evaluation
and efficacy testing
Thermo-stable animal vaccines
Contract Research services in any of the
above areas.
Rapid data collection and
assessment for Epidemiological
studies- GIS, mobile application
based data collection and
transmission, disease modeling
and using various imageries.DNA based molecular detection and
characterization of pathogens- Next
Generation Sequencing based genomics
studies.
Vaccine stabilization and novel
delivery formulations.
Efficacy testing in vivo
and in vitro models
Technologies-
Landscape Disease Study
Infectious Disease Dynamics and
Landscape Epidemiology Research
needed for the front end of vaccine
development and efficacy testing,
including disease burden analysis on
landscape level using GIS and modeling.
Eastern Development Region Jhapa | Morang | Sunsari
Central Development Region Kathmandu | Lalitpur | Bhaktapur | Chitwan
Western Development Region Nawalparasi
Mid-western Development Region Dang
Far-western Development Region Kailali
Respondents
Location
GPS coordinates
Site name
Level of education
Number of year operational
Number of years in the poultry business
Occupation (apart from poultry production)
Number of people in the household
Approximate family income
Poultry characteristics
Total number of birds
Category of poultry birds
Breed
Source of the birds
Interaction with wild birds
Management practices
Poultry housing type
Adequate housing provision
Location of coop/shed
Husbandry practices (intensive/free ranging)
Type of feeds given
Feeding and water container
Personal consumption/business
Bio-security practices:
Fencing
Multi-age birds
Mixed bird species
Multiple sources of stock birds
Other animals kept in poultry
Vaccination
Notification of death to authorities
Dealing with sick birds
Birds sold to
Sanitation practices:
Personal hygiene
Use of protective clothing (PPE)
Use of disinfectants
Poultry litter management
Disposal of dead birds
Knowledge of poultry diseases:
Awareness of bird flu outbreaks
Knowledge of different diseases
(AIV/NDV)
Symptomatic recognition of sick birds
Awareness of zoonotic capabilities
Source of information
Knowledge report sickness/death
35 Commercial Farms, 24 Backyard Farms, 2 Wild Birds Sites
Commercial farms – 525 birds, 3150 samples
Backyard farms – 72 birds, 432 samples
Wild birds – 10 samples
37%
3%
32%
22%
3% 3%
Commercial Chickens Sampled
Cobb 500
Cobb 700
Hyline Brown
Lohmann Brown
H&N Brown Nick
Giriraj
35%
25%
32%
0
5
10
15
20
25
30
35
40
Disinfectant Used PPE Used Vaccines Used
Bio-Security Practices
Newcastle (NDV) 83%
Influenza A (IA) 37%
Nation wide Sero-Prevalence
NDV’s prevalence of 83% - either the remaining farms did not vaccinate or there were
issues with vaccine efficacy as NDV vaccines are regularly prescribed by vets
Detection of IA by antibody ELISA is even more troublesome – 40% of the farms had
infected chickens (or history of infection)
Phylogenetic Analysis for Pathotypic position of isolated Newcastle Virus
Maximum likelihood Phylogenetic Tree constructed based on matrix gene of Newcastle disease virus validated by
bootstrap method (n=1000). Samples are labeled as, avian paramyxovirus type I_country of origin strain_Pathotype.
Sample isolated in this study are highlted in green and labelled at right side.
Sharing of Antimicrobial Resistance Genes Among Animals, Humans, and The Environment in Nepal:
A One Health Case Study
Sharing of Antimicrobial Resistance Genes Among Animals, Humans, and The Environment in Nepal:
A One Health Case Study
Sharing of Antimicrobial Resistance Genes Among Animals, Humans, and The Environment in Nepal:
A One Health Case Study
Sharing of Antimicrobial Resistance Genes Among Animals, Humans, and The Environment in Nepal:
A One Health Case Study
Sharing of Antimicrobial Resistance Genes Among Animals, Humans, and The Environment in Nepal:
A One Health Case Study