ladders give you choices for protein molecular weight · give you choices for protein molecular...

Thermo Scientific

protein ladders give you

choices for protein molecular weight estimation.

Thermo Scientific™ Pierce™ Prestained and Unstained Protein Ladders for easy, reliable protein molecular weight estimation.

In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com

In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca







http://www.thermoscientific.com

http://www.fishersci.com

Thermo Scientific SuperSignal Molecular Weight Protein LaddersThe Thermo Scientific™ SuperSignal™ Molecular Weight Protein Ladders contain a ready-to-use stabilized mixture of eight recombinant proteins ranging in size from 20 to 150kDa. These recombinant proteins bind antibodies used in the Western blot through an IgG binding site. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies. Use the SuperSignal Enhanced Molecular Weight Protein Ladders with mouse monoclonals.

150kDa

100kDa

80kDa

60kDa

50kDa

40kDa

30kDa

20kDa

4-12% Bis-Tris 4-20% Tris-glycine

5µL 10µL 5µL 10µL

Thermo Scientific Pierce Protein Molecular Weight MarkerThermo Scientific™ Pierce™ Prestained Protein Molecular Weight Markers are designed to monitor protein migration during SDS-polyacrylamide gel electrophoreses and monitor protein transfer onto membranes. Pierce Unstained Protein Weight Markers produce sharp bands on SDS-polyacrylamide gel following staining with Coomassie-based or silver stains and are recommended for accurate sizing of proteins on SDS PAGE and Western blots.

Thermo Scientific PageRuler Unstained Protein LaddersThermo Scientific™ PageRuler™ Unstained Protein Ladders are mixtures of recombinant, highly purified proteins designed for accurate sizing of proteins by SDS-PAGE. The ladders resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with Coomassie Blue or silver stain. Each ladder contains protein bands of greater intensity which serve as reference bands. Proteins can be detected on Western blots by staining with Ponceau S, Coomassie Blue, (except for 3.4kDa and 5kDa polypeptides) or by using Strep-Tactin™ conjugates.

Thermo Scientific PageRuler Prestained Protein LaddersThe Thermo Scientific™ PageRuler™ and PageRuler Plus Prestained Protein Ladders are comprised of highly purified recombinant proteins bound to three different chromophores for easy protein molecular weight determination. The green and orange reference bands in the PageRuler products provide easy orientation of the protein bands.

Thermo Scientific Spectra Multicolor Protein LaddersFour different chromophores bound to proteins in Thermo Scientific™ Spectra™ Multicolor Broad Range Protein Ladders produce a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range and Low Range Protein Ladders are designed for large and small protein analysis, respectively.

PageRuler PrestainedProtein Ladder

PageRuler PlusPrestained Protein Ladder

Prestained ProteinMolecular Weight Marker

Unstained ProteinMolecular Weight Marker

Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase

Bovine serum albumin

Ovalbumin

Lactate dehydrogenase

REase Bsp98I

β-lactoglobulinLysozyme

8-16% Tris-glycine SDS-PAGE

Product #’s 26612, 26613


Product #’s 26625, 266264-20% Tris-glycine SDS-PAGE



Product #’s 26614, 2661518% SP Tris-tricine SDS-PAGE

Product # 26632


Product #’s 26616, 26617, 26618 4-20% Tris-glycine SDS-PAGE


Product #’s 26610, 26611

Gel

Gel

Spectra MulticolorHigh Range Protein Ladder

Spectra MulticolorBroad Range Protein Ladder

PageRulerUnstained Protein Ladder

PageRuler Unstained Broad Range Protein Ladder

PageRuler Unstained Low Range Protein Ladder

Spectra MulticolorLow Range Protein Ladder

18% SP Tris-tricine SDS-PAGESP - Small Peptide

SDS - polyacrylamide gel with higher crosslinking, C=5%

Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa

Prestained or unstained, including specialty protein ladders. Choose what’s best for your application.

Thermo Scientific PageRuler Prestained NIR LadderThe Thermo Scientific™ PageRuler™ Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250kDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing. The protein MW markers in this ladder resolve into sharp bands when analyzed by SDS-PAGE and are labeled with a fluorescent dye for visualization with instruments equipped with near-infrared (NIR) fluorescence detection. The bands are also directly visible because the proteins are prestained blue.

PageRuler NIR Protein Ladder

4-20% Tris-glycine SDS-PAGEProduct # 26635

250

130

95

70

55

43

3426

15

11

–

–

–

–

––

––

–

–

kDa

Visi

ble

Ligh

t

Nea

r-IR

Imag

er

–

–

–

–

––

––

–

–

Our ready-to-use mixes of recombinant proteins facilitate easy protein analysis during gel electrophoresis and Western blotting. The Prestained Protein Ladders are recommended for approximate determination of molecular weight, monitoring the progress of the electrophoresis run and the efficiency of the protein transfer to the membrane during Western blotting procedures. For precise determination of molecular weights of target proteins in any buffer system, we recommend using our Unstained Protein Ladders.

Highlights:• Best value – high quality without the high price

• Performance – sharp protein band resolution and consistent migration patterns provide easy molecular weight determination

• Convenient – protein ladders are ready to load with no heating or boiling required

• Reliable – exceptional lot-to-lot consistency and reproducibility

Thermo Scientific




Product #’s PI84785, PI84786 Product # PI26635

Product # PI26610Product # PI26612Product # PI26614 Product # PI26630 Product # PI26632

Product #’s PI26616, PI26617 Product #’s PI26619, PI26620 Product #’s PI26623, PI26634 Product # PI26625Product # PI26628

BN03

3014

1 0

4/14

Prin

ted

in th

e U.

S.

thermoscientific.com/pierce© 2014 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only. Ready Gel is a registered trademark of Bio-Rad Laboratories, Inc. Strep-Tactin is a registered trademark of IBA GmbH. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.









In the United States:For customer service, call 1-800-766-7000To fax an order, use 1-800-926-1166To order online: www.fishersci.com

In Canada:For customer service, call 1-800-234-7437To fax an order, use 1-800-463-2996To order online: www.fishersci.ca

© 2013 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries.

http://thermoscientific.com

http://thermoscientific.com/pierce



http://www.fishersci.ca

Reach new heights in gel electrophoresis

0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300

Spectra Multicolor High Range

Spectra Multicolor Broad Range

PageRuler Unstained Broad Range

PageRuler Plus Prestained Ladder

PageRuler Unstained Ladder

PageRuler Prestained Ladder

Pierce Prestained Marker

Pierce Unstained Marker

PageRuler Unstained Low Range

Spectra Multicolor Low Range

Protein Ladder Range (kDa)

The bars indicate the ranges of each product. Diamonds indicate the location and color of protein bands in each product.

Unstained Ladder

Prestained Ladder

Selection Table for Thermo Scientific Pierce MW Markers and Protein Ladders

Reference Guide: Migration Patterns in Different Electrophoresis Conditions

Mig

ratio

n Pa

ttern

s of

Spe

ctra

Mul

ticol

or

High

Ran

ge a

nd L

ow R

ange

Pro

tein

Lad

der

Mig

ratio

n Pa

ttern

s of

Spe

ctra

Mul

ticol

or

Broa

d Ra

nge

Prot

ein

Ladd

erM

igra

tion

Patte

rns

of P

ageR

uler

Pr

esta

ined

Pro

tein

Lad

der

Mig

ratio

n Pa

ttern

s of

Pag

eRul

er P

lus

Pres

tain

ed P

rote

in L

adde

r

SP – small peptide SDS-polyacrylamide gel with higher degree of crosslinking (c=5% instead of usual ~3%).‡ ‡ – migration patterns were determined using respective Novex™ precast gels.††† – migration patterns were determined using respective Ready Gel™ precast gel.

% le

ngth

of g

el 40

25

15

1052

40

25

15

10

52

40

25

15

10

5

2

40

25

15

10

5

40

25

15

10

5

2

40

25

15

10

5

40

25

15

10

52

40

25

15

10

52

40

25

15

1052

40

25

15

105

40

15

1052

Thermo Scientific Spectra Multicolor Low Range Protein LadderGel type Tris-Tricine

Gel concentration 4-20% 10-20% 14% 16% 18% 14-SP % 16-SP % 18-SP % 10-20%‡ ‡ 16%‡ ‡ 10-20%† † †

Running buffer Tris-Tricine SDS

Apparent Molecular Weights (kDa)

10

20

30

40

50

60

70

80

90

100

% le

ngth

of g

el

300250

180

130

100

70

5040

300

250

180

130

100

300250180

130

100

70

50

40

300

250

180

130

100

70

5040

270205

150

120

85

65

50

40

270205150120

85

65

50

40

270185140115

80

65

50

40

300250180

130

100

70

50

40

300250180130100

70

50

40

Thermo Scientific Spectra Multicolor High Range Protein Ladder

Gel type Tris-Glycine Tris-Acetate†† Bis-Tris††

Gel concentration 4-12% 4% 6% 8% 10% 4-20% 3-8% 7% 4-12%Running buffer Tris-Glycine Tris-Acetate MOPS

Apparent Molecular Sizes (kDa)

10

20

30

40

50

60

70

80

90

100

% le

ngth

of g

el

260

140100

70

504035

25

15

10

260

14010070

50

40

35

25

15

10

260

140

100

70

50

40

35

25

15

260

140100

70

50

40

35

25

15

26014010070

50

4035

25

15

10

26014010070

50

40

35

25

15

10

225

120

85

65

40

50

3025

225

120

85

65

40

50

30

25

225

115

80

65

35

50

30

25

1510

225

11580

65

35

50

30

25

15

225

1158065

35

50

30

25

15

235

120

8070

40

50

30

25

15

10

235

1208070

40

50

30

25

15

10

235

1208070

40

50

30

25

15

10

260

140

100

70

50

4035

25

15

10

Thermo Scientific Spectra Multicolor Broad Range Protein Ladder


Gel concentration 4-20% 8-16% 10-20% 8% 10% 12% 15% 3-8% 7% 4-12% 10% 12%

Running buffer Tris-Glycine Tris-Acetate MOPS MES MOPS MES MOPS MES


10

20

30

40

50

60

70

80

90

100

205

120

85

65

30

50

25

250

100

70

35

55

25

15

130

190

11580

70

30

50

25

15

10

190

1158070

30

50

25

15

10

185

115

80

65

30

50

25

1510

250

100

70

35

55

25

15

10

130

250

10070

35

55

25

15

10

130

250

10070

35

55

25

15

10

130

250

100

70

35

55

25

15

130

250

10070

35

55

25

130

10

250

10070

35

55

25

15

130

10

205

120

85

65

30

50

25

185

1158065

30

50

25

15

185

1158065

30

50

25

15

190

1158070

30

50

25

15

10

% le

ngth

of g

el

15

Thermo Scientific PageRuler Plus Prestained Protein Ladder





10

20

30

40

50

60

70

80

90

100

17013010070

55

40

35

25

15

10

170

130

100

70

55

40

35

25

15

10

17013010070

55

4035

25

15

10

170130

100

70

55

40

35

25

15

170130100

70

55

40

35

25

15

10

17013010070

55

40

35

25

15

10

17013010070

55

40

35

25

15

10

150

120

85

65

50

40

3025

150

120

85

65

50

40

30

25

115140

80

65

50

40

30

25

15

10

1401158065

50

40

30

25

15

1401158065

50

40

30

25

15

1401158065

50

40

30

25

15

10

1401158070

50

40

30

25

15

10

1401158070

5040

30

25

15

10

Note: The apparent molecular size of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition.

†† – Migration patterns were determined using respective NuPAGE™ precast gels.

% le

ngth

of g

el

Thermo Scientific PageRuler Prestained Protein Ladder





10

20

30

40

50

60

70

80

90

100

Choosing the right protein ladder. Use this table as a quick reference guide to select the right protein ladder for your application.

Application

Thermo Scientific Pierce Protein Ladders SDS PAGE Western Blotting

Product Name Product # # ProteinsMW range

Reference bands


PI26630 11 5-250kDa 20, 50 and 100kDa

Best — — Best — — Good†

PageRuler Unstained Protein Ladder

PI26614 14 10-200kDa 50kDa Good — — Good — — Good†


PI26632 8 3.4-100kDa 25kDa Best — — Best — — Good†

PageRuler Unstained High Range Protein Ladder

PI26637 8 60-250kDa 150kDa Best — — Best — — Good†

Pierce Unstained Protein Molecular Weight Marker

PI26610 7 14.4-116kDa

— Good — — Good — — —

PageRuler Prestained Protein Ladder

PI26616, PI26617

10 10-170kDa Green 10kDa; Orange 70kDa

Good Good Good — Good‡ Good —

PageRuler Plus Prestained Protein Ladder

PI26619, PI26620

9 10-250kDa Green 10kDa; Orange 25 and 70kDa


Spectra Multicolor Broad Range Protein Ladder

PI26634, PI26623

10 10-260kDa Green 10 and 50kDa; Orange 40, 70 and 260kDa; Pink 140kDa

Good Best Best — Good‡ Best —

Spectra Multicolor Low Range Protein Ladder

PI26628 6 1.7-40kDa Green 10kDa; Orange 40kDa


Spectra Multicolor High Range Protein Ladder

PI26625 8 40-300kDa Green 50kDa; Orange 70 and 300kDa


Pierce Prestained Protein Molecular Weight Marker

PI26612 6 20-120kDa — Good Good Good — Good Good —

SuperSignal Molecular Weight Protein Ladder

PI84785, PI84786

8 20-150kDa — Good — — Good — Good Best

PageRuler Prestained NIR Protein Ladder

PI26635 10 11-250kDa 55kDa Good Good Good — Best Good —

† with Strep-Tactin™ HRP or AP Conjugates‡ Blue and green prestained bands fluoresce in NIR region

Unst

aine

d

Chemi. Lum.

NIR

Colo

rimet

ric

Pres

tain

ed

Prot

ein M

W

dete

rmin

ation

Prot

ein

band

visu

aliza

tion

Mon

itorin

g

elect

roph

ores

is

run Co

omas

sie/s

ilver

/

fluor

esce

nt

stain

ing

NIR

de

tect

ion

Mon

itorin

g pr

otein

trans

fer

Chem

ilum

ines

cent

band

visu

aliza

tion

thermoscientific.com/pierce


Ordering Information

Product # Description Pkg. Size

PI26610 Pierce Unstained Protein MW Marker 2 x 1mL

PI26612 Pierce Prestained Protein MW Marker 500µL

PI26614 PageRuler Unstained Protein Ladder 2 x 250µL

PI26616 PageRuler Prestained Protein Ladder 2 x 250µL

PI26617 PageRuler Prestained Protein Ladder 10 x 250µL

PI26619 PageRuler Plus Prestained Protein Ladder 2 x 250µL

PI26620 PageRuler Plus Prestained Protein Ladder 10 x 250µL

PI26634 Spectra Multicolor Broad Range Protein Ladder 2 x 250µL

PI26623 Spectra Multicolor Broad Range Protein Ladder 10 x 250µL

PI26625 Spectra Multicolor High Range Protein Ladder 2 x 250µL

PI26628 Spectra Multicolor Low Range Protein Ladder 250µL

PI26630 PageRuler Unstained Broad Range Protein Ladder 2 x 250µL

PI26632 PageRuler Unstained Low Range Protein Ladder 2 x 250µL

PI84785 SuperSignal Molecular Weight Protein Ladder 250µL

PI84786 SuperSignal Enhanced Molecular Weight Protein Ladder

250µL

PI26635 PageRuler Prestained NIR Protein Ladder 2 x 250µL

Reach new heights in gel electrophoresis

0 20 40 60 80 100 120 140 160 180 200 220 240 260 280 300

Spectra Multicolor High Range

Spectra Multicolor Broad Range

PageRuler Unstained Broad Range

PageRuler Plus Prestained Ladder

PageRuler Unstained Ladder

PageRuler Prestained Ladder

Pierce Prestained Marker

Pierce Unstained Marker

PageRuler Unstained Low Range

Spectra Multicolor Low Range

Protein Ladder Range (kDa)

The bars indicate the ranges of each product. Diamonds indicate the location and color of protein bands in each product.

Unstained Ladder

Prestained Ladder

Selection Table for Thermo Scientific Pierce MW Markers and Protein Ladders

Reference Guide: Migration Patterns in Different Electrophoresis ConditionsM

igra

tion

Patte

rns

of S

pect

ra M

ultic

olor

Hi

gh R

ange

and

Low

Ran

ge P

rote

in L

adde

r

Mig

ratio

n Pa

ttern

s of

Spe

ctra

Mul

ticol

or

Broa

d Ra

nge

Prot

ein

Ladd

erM

igra

tion

Patte

rns

of P

ageR

uler

Pr

esta

ined

Pro

tein

Lad

der

Mig

ratio

n Pa

ttern

s of

Pag

eRul

er P

lus

Pres

tain

ed P

rote

in L

adde

r

SP – small peptide SDS-polyacrylamide gel with higher degree of crosslinking (c=5% instead of usual ~3%).‡ ‡ – migration patterns were determined using respective Novex™ precast gels.††† – migration patterns were determined using respective Ready Gel™ precast gel.

% le

ngth

of g

el 40

25

15

1052

40

25

15

10

52

40

25

15

10

5

2

40

25

15

10

5

40

25

15

10

5

2

40

25

15

10

5

40

25

15

10

52

40

25

15

10

52

40

25

15

1052

40

25

15

105

40

15

1052

Thermo Scientific Spectra Multicolor Low Range Protein LadderGel type Tris-Tricine

Gel concentration 4-20% 10-20% 14% 16% 18% 14-SP % 16-SP % 18-SP % 10-20%‡ ‡ 16%‡ ‡ 10-20%† † †

Running buffer Tris-Tricine SDS

Apparent Molecular Weights (kDa)

10

20

30

40

50

60

70

80

90

100

% le

ngth

of g

el

300250

180

130

100

70

5040

300

250

180

130

100

300250180

130

100

70

50

40

300

250

180

130

100

70

5040

270205

150

120

85

65

50

40

270205150120

85

65

50

40

270185140115

80

65

50

40

300250180

130

100

70

50

40

300250180130100

70

50

40

Thermo Scientific Spectra Multicolor High Range Protein Ladder


Gel concentration 4-12% 4% 6% 8% 10% 4-20% 3-8% 7% 4-12%Running buffer Tris-Glycine Tris-Acetate MOPS


10

20

30

40

50

60

70

80

90

100

% le

ngth

of g

el

260

140100

70

504035

25

15

10

260

14010070

50

40

35

25

15

10

260

140

100

70

50

40

35

25

15

260

140100

70

50

40

35

25

15

26014010070

50

4035

25

15

10

26014010070

50

40

35

25

15

10

225

120

85

65

40

50

3025

225

120

85

65

40

50

30

25

225

115

80

65

35

50

30

25

1510

225

11580

65

35

50

30

25

15

225

1158065

35

50

30

25

15

235

120

8070

40

50

30

25

15

10

235

1208070

40

50

30

25

15

10

235

1208070

40

50

30

25

15

10

260

140

100

70

50

4035

25

15

10

Thermo Scientific Spectra Multicolor Broad Range Protein Ladder





10

20

30

40

50

60

70

80

90

100

205

120

85

65

30

50

25

250

100

70

35

55

25

15

130

190

11580

70

30

50

25

15

10

190

1158070

30

50

25

15

10

185

115

80

65

30

50

25

1510

250

100

70

35

55

25

15

10

130

250

10070

35

55

25

15

10

130

250

10070

35

55

25

15

10

130

250

100

70

35

55

25

15

130

250

10070

35

55

25

130

10

250

10070

35

55

25

15

130

10

205

120

85

65

30

50

25

185

1158065

30

50

25

15

185

1158065

30

50

25

15

190

1158070

30

50

25

15

10

% le

ngth

of g

el

15

Thermo Scientific PageRuler Plus Prestained Protein Ladder





10

20

30

40

50

60

70

80

90

100

17013010070

55

40

35

25

15

10

170

130

100

70

55

40

35

25

15

10

17013010070

55

4035

25

15

10

170130

100

70

55

40

35

25

15

170130100

70

55

40

35

25

15

10

17013010070

55

40

35

25

15

10

17013010070

55

40

35

25

15

10

150

120

85

65

50

40

3025

150

120

85

65

50

40

30

25

115140

80

65

50

40

30

25

15

10

1401158065

50

40

30

25

15

1401158065

50

40

30

25

15

1401158065

50

40

30

25

15

10

1401158070

50

40

30

25

15

10

1401158070

5040

30

25

15

10

Note: The apparent molecular size of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition.

†† – Migration patterns were determined using respective NuPAGE™ precast gels.

% le

ngth

of g

el

Thermo Scientific PageRuler Prestained Protein Ladder





10

20

30

40

50

60

70

80

90

100

Choosing the right protein ladder. Use this table as a quick reference guide to select the right protein ladder for your application.

Application

Thermo Scientific Pierce Protein Ladders SDS PAGE Western Blotting

Product Name Product # # ProteinsMW range

Reference bands


PI26630 11 5-250kDa 20, 50 and 100kDa

Best — — Best — — Good†

PageRuler Unstained Protein Ladder

PI26614 14 10-200kDa 50kDa Good — — Good — — Good†


PI26632 8 3.4-100kDa 25kDa Best — — Best — — Good†

PageRuler Unstained High Range Protein Ladder

PI26637 8 60-250kDa 150kDa Best — — Best — — Good†

Pierce Unstained Protein Molecular Weight Marker

PI26610 7 14.4-116kDa

— Good — — Good — — —

PageRuler Prestained Protein Ladder

PI26616, PI26617

10 10-170kDa Green 10kDa; Orange 70kDa


PageRuler Plus Prestained Protein Ladder

PI26619, PI26620

9 10-250kDa Green 10kDa; Orange 25 and 70kDa


Spectra Multicolor Broad Range Protein Ladder

PI26634, PI26623

10 10-260kDa Green 10 and 50kDa; Orange 40, 70 and 260kDa; Pink 140kDa


Spectra Multicolor Low Range Protein Ladder

PI26628 6 1.7-40kDa Green 10kDa; Orange 40kDa


Spectra Multicolor High Range Protein Ladder

PI26625 8 40-300kDa Green 50kDa; Orange 70 and 300kDa


Pierce Prestained Protein Molecular Weight Marker

PI26612 6 20-120kDa — Good Good Good — Good Good —

SuperSignal Molecular Weight Protein Ladder

PI84785, PI84786

8 20-150kDa — Good — — Good — Good Best

PageRuler Prestained NIR Protein Ladder

PI26635 10 11-250kDa 55kDa Good Good Good — Best Good —

† with Strep-Tactin™ HRP or AP Conjugates‡ Blue and green prestained bands fluoresce in NIR region

Unst

aine

d

Chemi. Lum.

NIR

Colo

rimet

ric

Pres

tain

ed

Prot

ein M

W

dete

rmin

ation

Prot

ein

band

visu

aliza

tion

Mon

itorin

g

elect

roph

ores

is

run Co

omas

sie/s

ilver

/

fluor

esce

nt

stain

ing

NIR

de

tect

ion

Mon

itorin

g pr

otein

trans

fer

Chem

ilum

ines

cent

band

visu

aliza

tion



Ordering Information

Product # Description Pkg. SizeU.S. Price

PI26610 Pierce Unstained Protein MW Marker 2 x 1mL $ 58

PI26612 Pierce Prestained Protein MW Marker 500µL $ 58

PI26614 PageRuler Unstained Protein Ladder 2 x 250µL $ 82

PI26616 PageRuler Prestained Protein Ladder 2 x 250µL $100

PI26617 PageRuler Prestained Protein Ladder 10 x 250µL $454

PI26619 PageRuler Plus Prestained Protein Ladder 2 x 250µL $122

PI26620 PageRuler Plus Prestained Protein Ladder 10 x 250µL $550

PI26634 Spectra Multicolor Broad Range Protein Ladder 2 x 250µL $106

PI26623 Spectra Multicolor Broad Range Protein Ladder 10 x 250µL $477

PI26625 Spectra Multicolor High Range Protein Ladder 2 x 250µL $138

PI26628 Spectra Multicolor Low Range Protein Ladder 250µL $120

PI26630 PageRuler Unstained Broad Range Protein Ladder 2 x 250µL $ 90

PI26632 PageRuler Unstained Low Range Protein Ladder 2 x 250µL $ 90

PI84785 SuperSignal Molecular Weight Protein Ladder 250µL $199

PI84786 SuperSignal Enhanced Molecular Weight Protein Ladder

250µL $199

PI26635 PageRuler Prestained NIR Protein Ladder 2 x 250µL $204

Thermo Scientific SuperSignal Molecular Weight Protein LaddersThe Thermo Scientific™ SuperSignal™ Molecular Weight Protein Ladders contain a ready-to-use stabilized mixture of eight recombinant proteins ranging in size from 20 to 150kDa. These recombinant proteins bind antibodies used in the Western blot through an IgG binding site. The protein markers can then be visualized either using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies. Use the SuperSignal Enhanced Molecular Weight Protein Ladders with mouse monoclonals.

150kDa

100kDa

80kDa

60kDa

50kDa

40kDa

30kDa

20kDa

4-12% Bis-Tris 4-20% Tris-glycine

5µL 10µL 5µL 10µL

Thermo Scientific Pierce Protein Molecular Weight MarkerThermo Scientific™ Pierce™ Prestained Protein Molecular Weight Markers are designed to monitor protein migration during SDS-polyacrylamide gel electrophoreses and monitor protein transfer onto membranes. Pierce Unstained Protein Weight Markers produce sharp bands on SDS-polyacrylamide gel following staining with Coomassie-based or silver stains and are recommended for accurate sizing of proteins on SDS PAGE and Western blots.

Thermo Scientific PageRuler Unstained Protein LaddersThermo Scientific™ PageRuler™ Unstained Protein Ladders are mixtures of recombinant, highly purified proteins designed for accurate sizing of proteins by SDS-PAGE. The ladders resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with Coomassie Blue or silver stain. Each ladder contains protein bands of greater intensity which serve as reference bands. Proteins can be detected on Western blots by staining with Ponceau S, Coomassie Blue, (except for 3.4kDa and 5kDa polypeptides) or by using Strep-Tactin™ conjugates.

Thermo Scientific PageRuler Prestained Protein LaddersThe Thermo Scientific™ PageRuler™ and PageRuler Plus Prestained Protein Ladders are comprised of highly purified recombinant proteins bound to three different chromophores for easy protein molecular weight determination. The green and orange reference bands in the PageRuler products provide easy orientation of the protein bands.

Thermo Scientific Spectra Multicolor Protein LaddersFour different chromophores bound to proteins in Thermo Scientific™ Spectra™ Multicolor Broad Range Protein Ladders produce a brightly colored ladder with an easy-to-remember pattern. The Spectra Multicolor High Range and Low Range Protein Ladders are designed for large and small protein analysis, respectively.





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDaG

el

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





B

lot

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa





Blo

t

Gel

Blo

t

Gel

Protein

β-galactosidase


Ovalbumin


REase Bsp98I



Product #’s 26612, 26613






Product # 26632




Product #’s 26610, 26611

Gel

Gel









Product #’s 26628, 26629

~260

~140

~100

~70

~50

~40~35

~25

~15

~10

kDa

–

–

–

–

–

––

–

–

–

–

–

–

–

–

––

–

–

–

~40~25

~15

~10

4.6

1.7

kDa

––

–

–

–

–

––

–

–

–

–

~120~ 85

~50

~35

~25

~20

––

–

–

–

–

kDa

116.0

66.2

45.0

35.0

25.0

18.414.4

–

–

–

–

–

––

kDa

~300~250

~180

~130

~100

~70

~50

~40

kDa

Gel

Blo

t

Gel

Blo

t

Gel

Gel

Gel

Gel

Blo

t

––

–

–

–

–

–

–

––

–

–

–

–

–

–

250150

100

705040

30

20

15105

––

–

–

––

–

–

–––

kDa

100

30 25 20 15

10

5

3.4

–

––––

–

–

–

kDa20015012010085706050

40

302520

15

10

––––––––

–

–––

–

–

kDa

~170

~130

~100

~70

~55

~40

~35

~25

~15

~10

kDa

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

–

~250

~130

~100

~70

~55

~35

~25

~15

~10

kDa

Prestained or unstained, including specialty protein ladders. Choose what’s best for your application.

Thermo Scientific PageRuler Prestained NIR LadderThe Thermo Scientific™ PageRuler™ Prestained NIR Protein Ladder is a mixture of 10 proteins (11 to 250kDa) that are blue-stained and fluor-labeled for near-IR fluorescent visualization and protein sizing. The protein MW markers in this ladder resolve into sharp bands when analyzed by SDS-PAGE and are labeled with a fluorescent dye for visualization with instruments equipped with near-infrared (NIR) fluorescence detection. The bands are also directly visible because the proteins are prestained blue.

PageRuler NIR Protein Ladder

4-20% Tris-glycine SDS-PAGEProduct # 26635

250

130

95

70

55

43

3426

15

11

–

–

–

–

––

––

–

–

kDa

Visi

ble

Ligh

t

Nea

r-IR

Imag

er

–

–

–

–

––

––

–

–

Our ready-to-use mixes of recombinant proteins facilitate easy protein analysis during gel electrophoresis and Western blotting. The Prestained Protein Ladders are recommended for approximate determination of molecular weight, monitoring the progress of the electrophoresis run and the efficiency of the protein transfer to the membrane during Western blotting procedures. For precise determination of molecular weights of target proteins in any buffer system, we recommend using our Unstained Protein Ladders.

Highlights:• Best value – high quality without the high price

• Performance – sharp protein band resolution and consistent migration patterns provide easy molecular weight determination

• Convenient – protein ladders are ready to load with no heating or boiling required

• Reliable – exceptional lot-to-lot consistency and reproducibility

Thermo Scientific




Product #’s PI84785, PI84786 Product # PI26635

Product # PI26610Product # PI26612Product # PI26614 Product # PI26630 Product # PI26632

Product #’s PI26616, PI26617 Product #’s PI26619, PI26620 Product #’s PI26623, PI26634 Product # PI26625Product # PI26628

BN03

3014

1 0

4/14

Prin

ted

in th

e U.

S.

thermoscientific.com/pierce© 2014 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only. Ready Gel is a registered trademark of Bio-Rad Laboratories, Inc. Strep-Tactin is a registered trademark of IBA GmbH. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries.









In the United States:For customer service, call 1-800-766-7000To fax an order, use 1-800-926-1166To order online: www.fishersci.com

In Canada:For customer service, call 1-800-234-7437To fax an order, use 1-800-463-2996To order online: www.fishersci.ca

© 2013 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries.

http://thermoscientific.com




http://www.fishersci.ca

ladders give you choices for protein molecular weight · give you choices for protein molecular...

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