Lactic acid microbiota in Mariona Rossell Garcia postpartum cow · Shigella NEONATAL CALF Faeces...
Transcript of Lactic acid microbiota in Mariona Rossell Garcia postpartum cow · Shigella NEONATAL CALF Faeces...
INTRODUCTION The bacteria genera most frequently isolated are:
Lactic acid microbiota in
postpartum cow and her calf
Mariona Rossell Garcia
June 2017
OBJECTIVES Determination of lactic acid bacteria in cow’s colostrum and milk, and the faeces of
their calves Comparison of the isolated microbiota
3 dairy farmsOsona
115 colostrum and milk samples 31 faeces samples
MATERIALS AND METHODSAgarManRogosaSharpewithcysteine(MRS+C)
Differentgeneraoflacticacidbacteria
SpecificforBifidobacteriumspp.
Aerobicconditions
Anaerobicconditions
Microaerophileconditions
AgarManRogosaSharpe(MRS)
37ºC24-48h
Incubationconditions Identificationmethods
GramStainCatalaseandOxidaseTest
API50CHL
RESULTS
CONCLUSIONS ✴ The genera isolated are Bifidobacterium spp., Lactobacillus spp. and Lactococcus spp. No many differences between samples and
farms ✴ The counts of the genera Bifidobacterium are higher in colostrum while the counts of Lactobacillus and Lactococcus are higher in milk ✴ The presence of the same genera between meconium and faeces supports the hypothesis of a non sterile gastrointestinal tract of the
neonate ✴ Exists a marked variability between individuals and cow’s quarters. For a representative sample of the individual, should be sampled
each of the quarters
Isol
atio
n fre
quen
cy
0 %
7,4 %
14,8 %
22,2 %
29,6 %
37 %
Lactic acid bacteria genera
Bifidobacterium spp. Lactobacillus spp. Lactococcus spp.
Colostrum Milk Faeces
Graphic1.Lacticacidbacteriageneraisolatedincolostrum,milkandfacessamples
32,95%
35,29%
33,33%
34,09%
35,29%
36,67%
32,95%
29,41%
30,00%
ColostrumandMilk
Kocuria Staphylococcus Streptrococcus Lactic acid bacteria Bifidobacterium, Lactobacillus, Lactococcus and Leuconostoc
PROBIOTICS??
Lactobacillus
Bifidobacterium
Escherichia
Shigella
FaecesNEONATALCALFDIARRHEA
Bifidobacteriumspp–Lactobacillusspp.andLactococcusspp.
LactobacillusplantarumLactococcuslactis
Colostrum=MilkFaecesProbiotics’features
MeconiumPossiblematernalorigin
Bifidobacteriumspp.ismorefrequentlyisolatedinmilksamples,butitscountsarehigherincolostrum
Colostrumandmilksamples:Highvariabilitybetweencow’squartersandbetweenindividuals
Faecessamples:Highvariabilitybetweenindividuals
Nomanydifferencesbetweenfarms
Graphic2.Representativeboxplotofcows’quarterscountofeachindividual,sortedbyfarms
Farm1Farm2Farm3