Laboratory Glassware. Final

7/25/2019 Laboratory Glassware. Final

1/25

Laboratory Glassware

Module 10

Kh. Farjana Urmi

Sr. QC O!er

"C# $ealthCare Limited


2/25

Laboratory glassware Laboratory glassware re%ers to a &ariety o% e'ui(ment)

traditionally made o% *lass) used %or s!ienti+! e,(erimentsand other wor- in s!ien!e) es(e!ially in !hemistry andbiolo*y laboratories.

Application:

Glass is relati&ely inert) trans(arent) heatresistant) and

easy to !ustomi/e. here are se&eral ty(es o% *lass) ea!hused %or dierent (ur(oses. 2orosili!ate *lass) whi!h is!ommonly used in rea*ent bottles) !an withstand thermalstress. Quart/ *lass !an withstand hi*h tem(eratures and istrans(arent in !ertain (arts o% the ele!troma*neti!

s(e!trum. 3ar-ened brown or amber 4a!tini!5 *lass) whi!h is!ommon in dar- stora*e bottles) !an blo!- ultra&iolet andin%rared radiation. $ea&ywall *lass) whi!h is !ommon in*lass (ressure rea!tors) !an withstand (ressuri/eda((li!ations.


3/25

Examples

here are many dierent -inds o% laboratory *lasswareitems. hese in!lude6

Beakersare sim(le !ylindri!al sha(ed !ontainers used tohold rea*ents or sam(les.

Flasks are narrowne!-ed *lass !ontainers) ty(i!ally!oni!al or s(heri!al) used in a laboratory to hold rea*entsor sam(les.

Bottles are !ontainers with narrow o(enin*s *enerallyused to store rea*ents or sam(les. Small bottles are !alled&ials.

Jars are !ylindri!al !ontainers with wide o(enin*s thatmay be sealed. 2ell jars are used to !ontain &a!uums.

Watch glasses are shallow *lass dishes used as an

e&a(oratin* sur%a!e or to !o&er a bea-er. Graduated cylinders are !ylindri!al !ontainers used %or

&olumetri! measurements. tirring rods are used to mi, !hemi!als. Burettes are used to dis(erse (re!ise amounts o% li'uid

rea*ents.

!ondensersare used to !ool hot li'uids or &a(ors.


4/25

Examples Funnelsare used to *et materials throu*h a narrow

o(enin*. "esiccators o% *lass !onstru!tion are used to drymaterials or -ee( material dry.

Glass tubes are !ylindri!al (ie!es o% *lassware usedto hold or trans(ort materials.

Glass retorts are used %or distillation. Glass pipettes are used to trans(ort (re!ise

'uantities o% 7uids. #est tubes are used by !hemists to hold) mi,) or heat

small 'uantities o% solid or li'uid !hemi!als) es(e!ially%or 'ualitati&e e,(eriments and assays

Glass $etri dishes are used to !ulture li&in* !ells. "rying pistols are used to %ree sam(les %rom tra!es

o% water) or other im(urities. Glass e%aporating dishes are used to e&a(orate

materials.

&icroscope slides are thin stri(s used to hold itemsunder a mi!ros!o(e.


5/25


6/25

Glassware

Glassware !an be di&ided into 8

*rou(s 9on&olumetri! *lassware

2ea-er

Flas-

:olumetri! Glassware:olumetri! Flas-

Graduated Cylinder

;i(ette

2urette


7/25

Glassware 4Cont..5

Beaker Used %or trans%errin* li'uid to another !ontainer or to

trans%er a small amount o% rea*ent %or use in(ro!edures.

:olume is not a!!urate) just an estimate.

9


8/25

:olumetri! Glassware

=e'uired %or $i*h "!!ura!y and ;re!ision =e'uired >hen ;re(arin* or Usin* Standard

Solutions

Graduated !ylinder For ra(id measurement o% li'uid &olume.

hey are *enerally more a!!urate and (re!ise %orthis (ur(ose than 7as-s.

his is a semia!!urate li'uid measurin* &essels.


9/25


>hat are the &olume o% water inthe *raduated !ylinder?

"lways measure the &olume o% a

li'uid atthe bottom o% the menis!us o% the

li'uid. 10 mL has a((ro, @.@8 mL

100 mL has a((ro, A8.B ml. $ere @ A8 ml are e,a!tly -nown

and the 0.@8 0.B ml is un!ertainbe!ause is was inter(olated orestimated.


10/25


4Cont..5

Graduated ;i(ette

Used for Routine Volume Measurements

of 25 mL or Less

>ide i(Use >hen Solids in

Sam(le


11/25

Graduated ;i(ette

'(# )(L*&E#+,!

>ide i(Use >hen Solids inSam(le


4Cont..5


12/25

:olumetri! Glassware 4Cont..5

$ipettes Laboratory instrument used to trans(ort a

measured &olume o% li'uid. hree ty(es o% *lass (i(ettes used in the laboratory

:olumetri!MohrSerolo*i!al

Serolo*i!al and Mohr (i(ettes ha&e dierentmar-in*s to assist in identi%yin* them as they areused dierently.

Serolo*i!al (i(ettes are 3 D to deli&er. oa!!urately dis(ense the measured &olume thelast bit must be blown out.

Mohr (i(ettes are C D to !ontain. hese(i(ettes are desi*ned to dis(ense the !orre!tlymeasured &olume) so there will be a minuteamount o% li'uid le%t in the ti(.


13/25


)olumetric $ipettes 3esi*ned to trans%er a +,ed amount o% li'uid when

+lled to the mar-) e.*. 10 mL and only 10 mL.

here is *enerally only one E+lllineE on a &olumetri!(i(ette.

For e,am(le a A mL &olumetri! (i(ette has onemar-in* on it. his mar-in* measures e,a!tly A mL. o%li'uid) no more) no less.


14/25

;ro(er Use o% ;i(ette

>hen readin* the &olume)"L>"S &iew the (i(ettedeadon at eye le&el withthe (i(ette held &erti!ally)(er(endi!ular to the *round.

;i(ettes are desi*ned to beused with a hand (um( orbulb) o% whi!h there aremany &arieties.

Never use your mouthwith a pipette!


15/25

;ro(er =eadin*

H

HO


16/25

Fill

By

Vacuum

Pipet

Bulb

Rinse


17/25


)olumetric Flask " volumetric fask is used to measure &ery

(re!isely one s(e!i+! &olume o% li'uid 4100 ml)8A0 ml) et!.) de(endin* on whi!h 7as- you use5.

his 7as- is used to (re(are a solution o% -nown

!on!entration. o ma-e u( a solution) +rst dissol&e the solid

material !om(letely) in less 7uid than re'uired to+ll the 7as- to the mar-.

"%ter the solid is !om(letely dissol&ed) &ery

!are%ully +ll the 7as- to the mL mar-. he to( is then sealed and the 7as- is in&erted

se&eral times to mi,.


18/25

#ypes o- )olumetric Glassware

eriali.ed/!erti0ed 1asks are !alibrated to Class "s(e!i+!ations.


19/25

!lass A 1asks are manu%a!tured to toleran!esestablished by"SM


20/25

:olumetri! Glassware Calibration

SO; on Calibration o% Glassware
http://sop%20on%20calibration%20of%20laboratory%20glassware/http://sop%20on%20calibration%20of%20laboratory%20glassware/


21/25

N10A1 CL


22/25

Glassware !leaners

>hen washin* *lassware) soa() deter*ent) or !leanin*(owder 4with or without an abrasi&e5 may be used.Cleaners %or *lassware in!lude "l!ono,) Li'uino,)Lu,) ide and Fab. he water should be hot. For*lassware that is e,!e(tionally dirty) a !leanin*

(owder with a &ery mild abrasi&e a!tion) su!h as2on"mi) will *i&e more satis%a!tory results. heabrasi&e should not s!rat!h the *lass.


23/25

Glassware !leaners 2!ont334

9otClean9otClean

BeadsBeads

ofof

LiquidLiquid


24/25

Sa%ety $andlin* o% Glassware

3o not (ut hot *lassware on !old or wet sur%a!es) or!old *lassware on hot sur%a!es. #t may brea- with thetem(erature !han*e.

3o not heat *lassware that is et!hed) !ra!-ed)!hi((ed) ni!-ed) or s!rat!hed. #t is more (rone to

brea-.

3o not heat *lassware dire!tly on ele!tri!al heatin*elements.


25/25

#hank 5ou

Laboratory Glassware. Final

Documents

Transcript of Laboratory Glassware. Final