Laboratory Equipment Lab Procedures January 12, 2010.

24
Laboratory Equipment Lab Procedures January 12, 2010

Transcript of Laboratory Equipment Lab Procedures January 12, 2010.

Page 1: Laboratory Equipment Lab Procedures January 12, 2010.

Laboratory Equipment

Lab Procedures

January 12, 2010

Page 2: Laboratory Equipment Lab Procedures January 12, 2010.

Centrifuge

• Used to separate substances of different densities that are in a solution.

• Supernatant: the liquid component of a sample.

• Sediment: the solid component of a sample

• Different types of centrifuges are used in veterinary medicine.

Page 3: Laboratory Equipment Lab Procedures January 12, 2010.
Page 4: Laboratory Equipment Lab Procedures January 12, 2010.

Microhematocrit Tubes

Page 5: Laboratory Equipment Lab Procedures January 12, 2010.

Blood Collection Tubes

• Different types of tubes are used depending on what type of sample is desired.

• Whole Blood- Purple (lavendar) top tube or green top tubes

Page 6: Laboratory Equipment Lab Procedures January 12, 2010.

Blood Collection Tubes Continued

• When looking at serum or other factors, use different type of tubes.

• Oxalate Tubes:

Page 7: Laboratory Equipment Lab Procedures January 12, 2010.

Blood Tubes Continued

• Citrate Tubes:

Page 8: Laboratory Equipment Lab Procedures January 12, 2010.

Blood Collection Equipment

• Use the largest gauge of needle that is comfortable for animal.

• As gauge size increases, needle diameter decreases.

Page 9: Laboratory Equipment Lab Procedures January 12, 2010.

Blood Collection Equipment Continued

• Syringes should be picked based on one closest to desired volume of blood.

• A larger syringe may collapse the vessel.

Page 10: Laboratory Equipment Lab Procedures January 12, 2010.

Blood Collection Continued

• Vacutainer allows blood to be collected directly into a blood collection tube.

Page 11: Laboratory Equipment Lab Procedures January 12, 2010.

Refractometer

• Total solids meter.• Used to measure the refractive

index of a solution. • Refraction is the bending of the light

rays as the pass from one medium into another.

• Refractometer in vet med is used to measure specific gravity of urine and plasma protein levels.

• Know how to calibrate refractometer

Page 12: Laboratory Equipment Lab Procedures January 12, 2010.

Blood Chemistry Analyzers/ CBC machines

• Generates data on organ function and complete blood counts.

• Machines count cells based on size of cells in a predetermined sample.

Page 13: Laboratory Equipment Lab Procedures January 12, 2010.
Page 14: Laboratory Equipment Lab Procedures January 12, 2010.

Incubators/ Agar plates

• Used in microbiological tests.• Sustains temperature settings (37 ˚

C)• Agar plates are inoculated with

potential bacteria.• Plates are smeared with inoculation

loops.

Page 15: Laboratory Equipment Lab Procedures January 12, 2010.

Pipettes

• Gives the ability to transfer liquids from one place to another.

• May be plastic or glass

Page 16: Laboratory Equipment Lab Procedures January 12, 2010.

Slides

• Thin sheet of glass used to hold objects for examination under a microscope.

• Usually place “specimen” in center of slide to view.

Page 17: Laboratory Equipment Lab Procedures January 12, 2010.

Cover Slips

• Smaller, sheet of glass that may cover specimen.

• Purposes are:• To protect microscope’s

objective lens from contacting the specimen

• Creates an even thickness for viewing.

Page 18: Laboratory Equipment Lab Procedures January 12, 2010.

Washing glassware

• Wash with soap, detergent, or cleaning powder.

• Wash with hot water.• Wash as quickly as possible after use. ZIf

a thorough cleaning is not possible immediately, put glassware to soak in water.

• All parts of glassware should be thoroughly scrubbed with a brush.

• Use brushes that will not scratch or abrade the glass surface.

Page 19: Laboratory Equipment Lab Procedures January 12, 2010.

Drying and Storing Glassware

• If glassware is to be dried, allow it to drain or use paper towels.

• Never tap glassware against the side of the sink!

• Dry labware by:• Hanging on wooden pegs or placing

them in baskets with mouths downward allowing them to dry in the air

• Protect glassware from dust by plugging mouth and storing in a dust free cabinet.

Page 20: Laboratory Equipment Lab Procedures January 12, 2010.

Technician Role

• Technician should have a thorough knowledge of all in-house laboratory equipment.• Know how to operate• Know requirements, calibration

measures, etc.

Page 21: Laboratory Equipment Lab Procedures January 12, 2010.

Sharps Container

• Always use an approved Sharps container to dispose of contaminated sharps.

• When 2/3 full dispose in compliance with Hazardous Waste Management Plan.

Page 22: Laboratory Equipment Lab Procedures January 12, 2010.

Recall the Metric System

Page 23: Laboratory Equipment Lab Procedures January 12, 2010.

Rules of Lab

• 1. NO FOOD OR DRINKS!!!• 2. Listen to instructions.• 3. Dispose of or clean all used

instruments and slides accordingly.• 4. Make sure that workstations are neat

and clean.• 5. Make sure to wear appropriate attire

and PPE when told.

Page 24: Laboratory Equipment Lab Procedures January 12, 2010.

• http://www.youtube.com/watch?v=aEjVSMeFHqs&feature=related