Laboratory Animal Services

2015 Induction and Orientation

Program

Laboratory Animal Services

2015 Induction and Orientation

Program Presenters:Andrew Bartlett (Manager)Nikki Reyne (Waite Facilities Coordinator)Pacita Wissell (Medical School Facility Coordinator)Tiffany Boehm (Health, Safety and Compliance Officer)Faye Gardner (Office Manager)Xavier Curlija (Admin. Officer)Denise Noonan (Animal Welfare Officer)

2.10pm Monday 23rd February 2015Stirling Lecture Theatre, Ground Floor Medical

School South

Vision and Values Policy Framework/Partnership LAS organisational structure Animal Welfare and Ethical Conduct

Animal Welfare Officer Administrative procedures/policies

Office Manager Standard Operating Procedures (SOPs)

Facility Coordinators Health, Safety and Compliance Question time

Program StructureProgram Structure

VisionVision

Laboratory Animal Services will support the University of Adelaide’s biomedical research community through the delivery of best practice animal

husbandry and the provision of world standard laboratory animal products and services, whilst

maintaining a commitment to the principles of the

AUSTRALIAN CODE FOR THE CARE AND USE OF ANIMALS FOR

SCIENTIFIC PURPOSES - 8TH EDITION 2013

Values/ObjectivesValues/Objectives

Laboratory Animal Services will…

…demonstrate a commitment to the purpose of the AUSTRALIAN CODE FOR THE CARE AND USE OF ANIMALS FOR SCIENTIFIC

PURPOSES, specifically, “…to ensure the ethical and humane care and use of animals used for scientific purposes”.

…strive for continuous improvement in animal husbandry through the training and development of the animal care officers and investigators.

…develop a customer focus by providing investigators with professional, timely and effective support.

Policy FrameworkBIOMEDICAL RESEARCH

Policy FrameworkBIOMEDICAL RESEARCH

The Client

PREVENTION OF CRUELTY TO ANIMALS ACT 1985

AUSTRALIAN CODE FOR THE CARE AND USE OF ANIMALS FOR SCIENTIFIC

PURPOSES - 8TH EDITION 2013

ANIMAL USERS HANDBOOK

ANIMAL ETHICS COMMITTEE

BIOMEDICAL ANIMAL RESEARCH ADVISORY GROUP

ANIMAL WELFARE OFFICE

LABORATORY ANIMAL SERVICES

PoliciesImportation/ExportTransportationAcquisitionQuarantineAnimal housing standardsTrainingBreeding/Weaning (rodents)

ProtocolsAnaesthesiaEuthanasiaContingency plansSPF rodent productionAnimal health surveillanceAEC applicationsSecurity procedures

Standard Operating Procedures(SOPs)

Research Integrity + Animal Welfare

Traffic flowsAnimal husbandry

Technical proceduresFacility maintenance

Housekeeping

The “Partnership”The “Partnership”

Animal Technician and the Biomedical Researcher

Priorities AT BmRAnimal Welfare Research Integrity Customer Service Effective Communication

The complexity of the environment in which we work can distort our perspectives at a given

point in time BUT our objectives should always be the same!!

Laboratory Animal Services 2015 Organisational Structure

Pro-Vice Chancellor(Research Operations)

Richard Russell AM

Animal Welfare Officer11154 Denise Noonan (HEO9)

Manager, Laboratory Animal Services4642 Andrew Bartlett (HEO10)

Advisory Groups

Facility Coordinator: Waite Campus2428 Nikki Reyne (HEO6)

Facility Coordinator: Medical School2775 Pacita Wissell (HEO6)

Senior Animal Technician14274 Luke Ragless (HEO4)

Senior Animal Technician14075 Melissa Thomas (HEO4)

Senior Animal Technician1909 Rebecca Arbon (HEO4

Senior Animal Technician2425 Sarah Fisher (HEO4)

Senior Animal Technician3104 Alice Parmiter (HEO4)

Office Manager2876 Faye Gardner (HEO5)

Animal Technician10955 Sue Pinkus (HEO3)

Animal Technician4026 Nathan Stringer (HEO3)

Animal Technician16707 Despina Livadiotis (HEO3)

Health, Safety & Compliance Officer17269 Tiffany Boehm (HEO5)

Animal Technician16696 Faye Bone (HEO3)

Senior Animal Technician4027 Chantal Didenko (HEO4)

Animal Technician 15805 Bud Duncan (HEO3)

Animal Technician16874 Jaimee Spurr (HEO3)

Senior Animal Technician11307 Xavier Curlija (HEO4)

Senior Animal Technician13983 Jessica Church (HEO4)

Animal Technician4615 Paige Farrow (HEO3)

Administrative Officer12925 Susan Smith (HEO3)

Animal Welfare Officer

Dr Denise Noonan Mobile: 0421 615 147 Phone: 831 34107 E-mail: denise.noonan@adelaide.edu.au Office: DV-C & V-P Research, Level 14, 115

Grenfell St. http://www.adelaide.edu.au/animalwelfar

e/

Documents relevant to scientific use of animalsDocuments relevant to scientific use of animals

http://www.nhmrc.gov.au/guidelines-publications/ea28 http://www.australiananimalwelfare.com.au/home

http://www.legislation.sa.gov.au/LZ/C/A/Animal%20Welfare%20Act%201985.aspx

Animal Welfare Officer Duties

Promote awareness of and adherence to the Australian Code for the Care and Use of Animals for Scientific Purposes (2013) http://www.nhmrc.gov.au/guidelines-publications/ea28

Promote the 3 Rs –Replace, Reduce, Refine Provide clinical veterinary services for research

animals Monitor animal holding facilities Provide advice to

Researchers Animal Care Staff The University Animal Ethics Committees

University of Adelaide processes regarding approval for the ethical use of animals

University of Adelaide processes regarding approval for the ethical use of animals

Animal Ethics Committees Medical Committee Secretary: Tim Anson (ext. 32980) Science Committee Secretary: Helen Malby (ext. 34014) http://www.adelaide.edu.au/ethics/animal/

Compulsory Animal Ethics Induction & Refresher Training Courses

The use of animals in science: ethical considerations University of Adelaide North Terrace, Bonython Hall Wednesday 11th March 2014, 8.30am-1.00pm &

2pm-4pm Register on-line TODAY would be appreciated for

planning purposes! http://www.adelaide.edu.au/ethics/animal/training/

Optional Practical Training in common lab animal procedures -Handling, injection, blood collection

Mouse, Rat and Introduction to Anaesthesia and Surgery of Rodents

Venue: Med School South Teaching Laboratory S210 Register on-line TODAY link for on-line registration at http

://www.adelaide.edu.au/animalwelfare/training-workshops/

(All queries to Dr Denise Noonan)

Australian CodeAustralian Code

Responsibilities of Investigators and Teachers

Personal responsibility for all matters relating to the welfare of their animals. the responsibility of investigators

extends over all facets of the care and use of animals in projects approved by the ethics committee.

written AEC approval must be obtained before a project can begin, all AEC directives must be complied with

Australian CodeAustralian Code

Responsibilities of Investigators and Teachers

Records of animal use and monitoring must be maintained and available for audit, including:

Clinical Record Sheets Details of animal treatments including

anaesthetics, analgesics, experimental treatments and doses given

Numbers of each species and strain of animal used

Breeding colony production records Post mortem findings and adverse

event reports

Australian CodeAustralian CodeResponsibilities of Investigators and Teachers

Clinical Record Sheets to be held in the room where the animals are held throughout their scientific use.

LABORATORY ANIMAL SERVICES

CLINICAL RECORD SHEET (RAT & MOUSE)

RESEARCHER NAME: _________________________ ID NUMBER: ______________________________________ DEPARTMENT: ______________________________ ANIMAL ETHICS NUMBER: __________________________

DA

TE

DU

LL

/RU

FF

LE

D

CO

AT

PO

OR

PO

ST

UR

E/

HU

NC

HE

D

PA

LE

OR

SU

NK

EN

E

YE

S

CH

AN

GE

IN

B

EH

AV

IOU

R

RE

DU

CE

D F

OO

D/

WA

TE

R IN

TA

KE*

DE

HY

DR

AT

ION

DIA

RR

HO

EA

SQ

UE

AL

ING

W

HE

N H

AN

DL

ED

RE

LU

CT

AN

T T

O

MO

VE

WE

IGH

T L

OS

S

OT

HE

R

CO

MM

EN

TS

TO

TA

L S

CO

RE

SIG

NE

D

YES = 1 Scores of 3 require the animal to be checked 3 times per day NO = 0 Scores of higher than 3 require Animal Welfare/ Veterinary advice Scores higher than 4 require Euthanasia

* Where known in single housed animals

Key Resource: Animal User’s Handbook

http://www.adelaide.edu.au/ethics/animal/guidelines/animalusershandbook/

Global Context: Animal Activism

Responses to Animal Activism

UK Responses to Animal Activism

Administrative Support

Faye Gardner Office Manager

Sue Smith Administrative Assistant

Xavier Curlija Administrative Assistant

Ph. 35340Fax. 34342Room: S643Email:las.manager@adelaide.edu.au

Administrative ProceduresAdministrative Procedures

General Issues Web page http://www.adelaide.edu.au/animal_services/

Communication channels interpersonal methods stakeholder register Blog

Administrative ProceduresAdministrative Procedures

Involving animal use: Ethics approval Animal ordering/ technical assistance requests

http://www.adelaide.edu.au/animal_services/products/ordering.html

Calcium (Room/Equipment Booking system) http://www.adelaide.edu.au/animal_services/

Fee structure Internal/external clients

Invoicing

Australian CodeSection 2. Responsibilities

Australian CodeSection 2. Responsibilities

The Facility Manager Responsibilities are: obtain written approval from the AEC for activities

related to care and management of animals in the facility and regularly review these activities.

ensure activities are implemented and conducted ensure all people involved in animal care

understand their responsibilities seek AWO advice and ensure advice given is

followed Implement QA programs and regularly review these

programs in accordance with current best practice Coordinate between researchers and LAS staff Report to the AEC

Australian CodeChapter 2.5, Responsibilities

Australian CodeChapter 2.5, Responsibilities

The Animal Technician Responsibilities are: support animal wellbeing avoid or minimise harm, including

pain and distress ensure provisions for animals at the

conclusion of their use and disposal of carcasses and waste material

ensure records are maintained

Medical School FacilityMedical School Facility Standard Operating Procedures

1. Traffic Flows

- PC2 Barrier North (Clean Rooms & Bio-Exclusion)- PC2 Barrier South (Bio-Exclusion & Bio- Containment)- PC2 Isolation Room 638a- Conventional (Rodents + PC1 rodent room)

- Conventional (Sheep)

- Disciplinary action for breach of

protocol

Medical School FacilityMedical School Facility

Standard Operating Procedures (cont…)

2. Gowning requirements

3. Theatre/Anaesthetic Machine Booking

4. House keeping

5. Animal Care

6. Animal Handling

Medical School Facility

Technical Assistance – what the Medical School Facility can provide

Maintain GMO and inbred breeding colonies to OGTR regulations.

Tail biopsies Time mating (including plug checking) Identification Orbital eye/facial vein blood collection Cardiac blood collection Gavaging S/C, I/M, I/P injections of all species Tail vein injection Jugular vein injecting (sheep) Anaesthetising all species (including pre and post operative

care)

Waite Campus Animal Facilities

Waite Campus Animal Facilities

SPF Barrier Production Inbred Strains available: C57/Bl6,

BALB/C, CBA, Nude, & DA Outbred: Swiss, SD, AW, HW Hybrid: CBA F1 Mice available from birth to 8-

10weeks Rats available from birth to 6

weeks Large orders – advance notice Standing and advance orders are

given priority Ordering by age / weight

nikki.reyne@adelaide.edu.auPh.: 37363

Waite Campus Animal Facilities

SPF Barrier Production Time Matings

3 days notice prior to mating Day 0 is plug day No guarantee that the animals

will mate on the specified day No guarantee that the animals

will be pregnant when a plug is found

QA Program routine 6 weekly testing results available online

Roseworthy Campus Animal Facilities

Livestock Research Centre (LRC) 56 pens Additional 20 pens able to

be converted to 10 double size pens

Surgical suite Booking of pens through LAS

Small Animal Research Facility

3 animal holding rooms plus procedure room

Compliance

OGTR (Office of the Gene Technology Regulator)

AQIS (Australian Quarantine Inspection Service)• DAFF (Department of Agriculture, Fisheries and

Forestry)

Legal and Risk

Health and Safety

Genotrack

Office of the Gene Technology Regulator -

OGTR Cage Cards Must include IBC Number

Online training

PC1 Medical School Conventional Room 658 and 644

PC2 Medical School All Barrier and Containment Rooms Post Mortem Room 654 Isolation Room 638

Gowning Requirements

http://www.ogtr.gov.au/

Department of Agriculture, Fisheries and Forestry –

DAFFFormally Australian Quarantine Inspection Service (AQIS) AQIS import certificate required before import

Notification to Facility Co-ordinator Current Ethics approval OGTR licence IBC Number (if Genetically Modified) In-house Import Form Health reports

Waite Campus Xenopus room

Medical School Room 632a Barrier Containment and Isolation

Room 638

Legal Compliance Animal Welfare Act (1985)

Controlled Substances Act (1984)

Dangerous Substances Act (1979)

Gene Technology Act (2000)

Livestock Act (1997)

Quarantine Act (1908)

Radiation Protection and Control Act (1982)

Health, Safety and Wellbeing

Health, Safety and Wellbeing

Branch Policy

Hazards associated with animal work manual handling bites, scratches and kicks research activity

Evacuation Procedures animal experiments alarms emergency exits

Client Responsibilities

Evacuation Points

Genotrack Breeding Colony Maintenance Software

breeding weaning matings statistics ethics health care

Web based software need to be issued with a licence and

password

Available for researchers to use

QuestionsQuestions

…hmmmmm?