Lab Meeting 10-18-13. Tet Tet+ 0.1mM Caffeine Tet+ 0.1mM Theophylline Results of Single Colony That...
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Lab Meeting 10-18-13
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Transcript of Lab Meeting 10-18-13. Tet Tet+ 0.1mM Caffeine Tet+ 0.1mM Theophylline Results of Single Colony That...
Lab Meeting
10-18-13
Tet Tet+ 0.1mM Caffeine Tet+ 0.1mM Theophylline
Results of Single Colony That Grew on Tet + Caffeine
Grown at 25C for 4 days
Results: ecdm8 + TetA
Tet Tet+ 0.1mM Caffeine Tet + 0.1mM Theophylline
Grown at 25C for 4 days
Disks of Theo or Caffeine on J303/4C5 + J79/1A2
Disks of Theo or Caffeine on J303/4C5 + J79/1A230 Degrees C
Next Steps
• Continue Running Experiment at 30C for 2 days
• Finishing cloning ecdm8 downstream of TetA
![Two Types of Tet-On Transgenic Lines for Doxycycline ......(Tet)- or doxycycline (Dox)-inducible Tet-On system [10,11] has been used in zebrafish to conditionally control Tet-responsive](https://static.fdocuments.in/doc/165x107/5f7b76c185c7f11b071fcfbc/two-types-of-tet-on-transgenic-lines-for-doxycycline-tet-or-doxycycline.jpg)
