LAB FOUR Photosynthesis
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Transcript of LAB FOUR Photosynthesis
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LAB FOUR Photosynthesis
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4A Plant Pigment Chromatography
4B Tracking the Light Reaction
LAB FOUR Photosynthesis
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•How is the light reaction affected by the presence or absence of light?
•How is it affected by the boiling of chloroplasts?
LAB FOUR Photosynthesis
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Isolated chloroplasts
LAB FOUR Photosynthesis
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Isolated chloroplasts
LAB FOUR Photosynthesis
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boiled unboiled
LAB FOUR Photosynthesis
Isolated chloroplasts
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boiled unboiled
LAB FOUR Photosynthesis
Isolated chloroplasts
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boiled unboiled
LAB FOUR Photosynthesis
Isolated chloroplasts
light
dark
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LAB FOUR Photosynthesis
Isolated chloroplasts
DPIP
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LAB FOUR Photosynthesis
Isolated chloroplasts
DPIP
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LAB FOUR Photosynthesis
Isolated chloroplasts
boiled unboiled boiled unboiled
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LAB FOUR Photosynthesis
Isolated chloroplasts
boiled unboiled boiled unboiled blank
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LAB FOUR Photosynthesis
Isolated chloroplasts
DPIP replaces NADP+ in the light reactions of photosynthesis.
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LAB FOUR Photosynthesis
Isolated chloroplasts
DPIP replaces NADP+ in the light reactions of photosynthesis.
As DPIP is reduced, it turns from blue to colorless.
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LAB FOUR Photosynthesis
Isolated chloroplasts
DPIP replaces NADP+ in the light reactions of photosynthesis.
As DPIP is reduced, it turns from blue to colorless.
A spectrophotometer measures the absorption of blue light.
0%
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LAB FOUR Photosynthesis
Isolated chloroplasts
DPIP replaces NADP+ in the light reactions of photosynthesis.
As DPIP is reduced, it turns from blue to colorless.
A spectrophotometer measures the absorption of blue light.
10%
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LAB FOUR Photosynthesis
Isolated chloroplasts
DPIP replaces NADP+ in the light reactions of photosynthesis.
As DPIP is reduced, it turns from blue to colorless.
A spectrophotometer measures the absorption of blue light.
40%
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LAB FOUR Photosynthesis
Isolated chloroplasts
DPIP replaces NADP+ in the light reactions of photosynthesis.
As DPIP is reduced, it turns from blue to colorless.
A spectrophotometer measures the absorption of blue light.
80%
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Cuvettes:
1.Blank (H2O only)
2.In the dark
3.Normal
4.Boiled chloroplasts
5.No chloroplasts
LAB FOUR Photosynthesis
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LAB FOUR Photosynthesis
1 2 3 4 5Light Yes No Yes Yes Yes
Buffer 1 mL 1 mL 1 mL 1 mL 1 mL
H2O 4 mL 3 mL 3 mL 3 mL 3 mL + 3 drops
DPIP 1 mL 1 mL 1 mL 1 mL 1 mL
Chloro-plasts
3 drops 3 drops 3 drops 3 drops boiled
No
Cuvettes:
Keep all cuvettes on ice!
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Adjust empty spectrophotometer to 0%
Adjust with blank cuvette to 100%
Add 3 drops chloroplast to your cuvette and immediately measure transmittance.
Measure again at 5, 10, and 15 minutes.
LAB FOUR Photosynthesis
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•Five teams will handle one cuvette each•Each team will take four measurements•Your turn lasts exactly one minute.
LAB FOUR Photosynthesis
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LAB FOUR Photosynthesis
1 2 3 4 5Light Yes No Yes Yes Yes
Buffer 1 mL 1 mL 1 mL 1 mL 1 mL
H2O 4 mL 3 mL 3 mL 3 mL 3 mL + 3 drops
DPIP 1 mL 1 mL 1 mL 1 mL 1 mL
Chloro-plasts
3 drops 3 drops 3 drops 3 drops boiled
No
Cuvettes:
Keep all cuvettes on ice! .
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Adjust empty spectrophotometer to 0%
Adjust with blank cuvette to 100%
Add 3 drops chloroplast to your cuvette and immediately measure transmittance.
Measure again at 5, 10, and 15 minutes.
.
LAB FOUR Photosynthesis