Honors Biology Arizona College Prep

Mrs. Glassmeyer Textbook Reference: 4.10-4.14, The Living

World 7th edition

Lab: Diffusion & Osmosis

Unit Learning Goal: Student will be able to compare structure & analyze transport of materials into and out of prokaryotic & eukaryotic cells by accurately explaining the role of proteins & ATP.

Lab Learning Goal(s):

1. To investigate diffusion through student selected experimental setups.

2. To investigate osmosis through student selected experimental setups.

3. To predict the movement of water in experimental setups.

Learning Goals

Review: Solution & Cell Basics

Which part is which?

Solute

Solvent

How about….

Cytoplasm

Extracellular fluid

What are the Osmotic Concentrations?

Hypertonic – higher solute concentration and lower water concentration

Hypotonic – lower solute concentration and higher water concentration

Isotonic – equal solute concentration

Osmosis in Plant Cells

Onion Cells in Hypotonic Solution Onion Cells in Hypertonic Solution

How to do an Osmosis Problem?

Let’s observe a problem being solved.

Write out the steps for solving a problem in your own words.

Let’s apply to four sample problems.

Sample Problems

Provided in class.

Osmotic Situations

Let’s create and fill out a table for reference

Extracellular Fluid (Solution) Concentration

Cytoplasm (Cell) Concentration

Water Moves? Cell Does What?

Hypertonic

Hypotonic

Isotonic

Osmotic Situations

Check your chart

Now, what does this actually look like?

Extracellular Fluid (Solution) Concentration

Cytoplasm (Cell) Concentration

Water Moves? Cell Does What?

Hypertonic Hypotonic Out of the cell Shrinks

Hypotonic Hypertonic Into the cell Swells

Isotonic Isotonic Both directions No change