Lab 5

Sodium Dodecyl Sulfate (SDS)- Polyacrylamide Gel Electrophoresis (PAGE)

Gel Electrophoresis/

Technique that relies on an electric field to separate molecules based on their size and charge

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SDS-PAGE SDS = detergent

Polyacrylamide Gel Electrophoresis

Tertiary Structure

Quaternary Structure

Sample Buffer: SDS Bromphenol Blue Glycerol

Reducing Sample Buffer: SDS Bromphenol Blue Glycerol β-mercaptoethanol

Loading the Gel

Sample Preparation

Relative Mobility

Semi-Log Graph Paper

RM

MW

/Da

1x 103

1x 104

1x 105

1x 106

Enztme-Linked Immunosorbant Assay ELISA

Terminology

o  Immunology o  Antigen o  Antibody

Antibodies as vital tools in scientific research, in biotechnology and in diagnosing and treating diseases

ELISA Relies on antibodies to detect foreign entities in liquid samples

Disease detection Pregnancy tests And many others…

ELISA

Immuno Sorbant

Surface/ Polystyrene

Antigen-Antibody Complex

Antibodies Recognize Specific Antigens

Constant region is not involved in antigen binding!

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