Cytology 244 First Term of year 1432-1433 Nuha AL-Abdulhadi lab 8.
Lab 1st year cytology
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Transcript of Lab 1st year cytology
Cytology 1
1. Single mitochondria, RER & Lipid droplet.2. Mitochondria ,RER & Secondery
lysosomes.3. Mitochondria, SER & glycogen granules.4. T.S of centriole , Golgi complex &coated
vesicle.5. Golgi complex.6. Peroxisome & Golgi complex.7. Golgi apparatus in epididymis
Cytology 2
8. Nucleus & RER (EM).9. Nuclear pore & rER continuous with the
outer nuclear membrane(EM).10. Melanin pigment in negroid skin11. Glycogen granules in liver. Slide12. lipid droplet.(EM)
Single mitochondria, rER
& lipid droplet
Mitochondria .Outer membrane and inner folded membrane.
Cristae Rough endoplasmic reticulum :
Consists of stacks of flattened cisternae.Membranous organells .
Polyribosomes on cytosolic surface of the membrane
Mitochondria, rER & secondary lysosomes
Secondary lysosomes. Membranous
cell organelles..Appears heterogenous due to presence of digested materials
sER , Mitochondria &
glycogen granules
Smooth endoplasmic reticulum. Absence of ribosomes (smooth)Cisternae is tubular in shape (notflattened(
Mostly found in cells synthesizing steroid hormones, cholesterol and triglycerides like liver and endocrine cells.
Glycogen granules: appears as collections
of electron-dense particles (rosett appearance)
T.S. of centriole, Golgi
complex & coated vesicle
Centrioles are non-membranous organelles.
centriole is a hollow cylinder with a dense wall around an electron lucent central core.
Its wall is made up of 9 bundles of microtubules, each bundle consists of 3 microtubules (triplets).
Golgi complex
Cis face and trans face, transfer vesicles secretory vesicles.
Cis-face (immature or forming face) which is usually convex. It is directed towards rough endoplasmic reticulum and its cisternae are highly fenestrated.
Trans-face (mature or secretory face) is usually concave and is directed towards the plasmalemma. The lumen of its cisternae tends to be wider.
Peroxisome & Golgi complexSpherical membranous organelles.Have a dense central region called crystaline core.Fine granular homogenous content.
Glycogen granules in liverA cytoplasmic inclusion, present in large amounts in liver cells (hepatocytes). Glycogen appears as dark acidophilic granules