The Respiratory System & The Respiratory System & SpirometrySpirometry

Lab 6Lab 6

Lab 6 ActivitiesLab 6 Activities Identify parts of the respiratory systemIdentify parts of the respiratory system

– Respiratory tree, thoracic organs, and larynx Respiratory tree, thoracic organs, and larynx modelsmodels

– Fresh lung (if available)Fresh lung (if available)

Examine histology of the:Examine histology of the:– TracheaTrachea– LungLung

Examine the operation of the lung modelExamine the operation of the lung model

Biopac L012-Lung-1:  Spirometry Biopac L012-Lung-1:  Spirometry – Measure lung volumes and capacitiesMeasure lung volumes and capacities

Respiratory System StructureRespiratory System Structure1.1. Conduction zone: Conduction zone:

pathway for pathway for pulmonary pulmonary ventilationventilation

2.2. Respiratory zone: Respiratory zone: membrane for gas membrane for gas exchange exchange external respirationexternal respiration

Clinically, two Clinically, two parts:parts:

– Upper respiratory Upper respiratory tracttract

– Lower respiratory Lower respiratory tracttract

Respiratory System StructureRespiratory System StructureHistology reflects the Histology reflects the

different functions different functions of the different parts of the different parts of the systemof the system

Position in respiratory Position in respiratory pathway determines pathway determines cell typecell type

non-keratinized non-keratinized stratified squamousstratified squamous

pseudostratified pseudostratified ciliated columnarciliated columnar

cuboidal ciliatedcuboidal ciliated simple squamous / Type I simple squamous / Type I Alveolar cellsAlveolar cells

Pseudostratified Columnar EpitheliumPseudostratified Columnar Epithelium

Lamina Propria

Hyaline Cartilage

mucosa

Submucosa

seudostratified epithelium

= lamina propria

ucous glands

mooth muscle

artilage

Gross Section Through LungGross Section Through Lung

Smoker’s LungsSmoker’s LungsNon-smoker

Small BronchioleSmall Bronchiole

Lung Tissue slide Lung Tissue slide Respiratory Bronchiole

Alveolar Duct

Alveoli

Alveolar Sac

Capillaries in Alveolar WallCapillaries in Alveolar Wall

SpirometrySpirometry

SPIROMETRY –SPIROMETRY – RESPIRATORY VOLUMESRESPIRATORY VOLUMES

AND CAPACITIES AND CAPACITIES

Total Lung CapacityTotal Lung Capacity = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV) = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV) + Expiratory Reserve Volume (ERV) + Residual Volume (RV)+ Expiratory Reserve Volume (ERV) + Residual Volume (RV)

Vital CapacityVital Capacity = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV) + = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV) + Expiratory Reserve Volume (ERV)Expiratory Reserve Volume (ERV)

Inspiratory CapacityInspiratory Capacity = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV) = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV)

Functional Residual CapacityFunctional Residual Capacity = Expiratory Reserve Volume (ERV) + = Expiratory Reserve Volume (ERV) + Residual Volume (RV)Residual Volume (RV)

Spirometry Spirometry – –

CalibrationCalibration

Insert the calibration syringe/filter assembly into the side of the airflow transducer labeled "Inlet." 

Inlet

Wait for 8 seconds of no air flow

Inlet

Experimental TreatmentsExperimental Treatments

1.1. 4 normal breaths4 normal breaths

2.2. Inhale as deeply as possibleInhale as deeply as possible

3.3. Exhale to normal depthExhale to normal depth

4.4. 3 normal breaths3 normal breaths

5.5. Exhale as much as possibleExhale as much as possible

6.6. 3 normal breaths3 normal breaths

7.7. Inhale as much as possibleInhale as much as possible

8.8. Exhale as fast and completely as possibleExhale as fast and completely as possible

9.9. 4 normal breaths4 normal breaths

BioPac Spirogram Example DataBioPac Spirogram Example Data

3 regularbreaths

3 regularbreaths

3 regularbreaths

1 strongestinhale

1 regular exhale

1 strongestinhale

1 strongest exhale 1 strongest exhale

This part is in addition to what Biopac Lesson 12-Lung 1 prompts you to do!

Do Data Analysis on the lower red Volume curve.

Summary of Class DataSummary of Class Data

Link to Table

The EndThe End

Lab 6Lab 6

Biopac SpirometryBiopac Spirometry

Residual volume

SpirometrySpirometry