L. rhamnosus SP 1 AND CARIES

Dental caries, or tooth decay, is one of the world's most widespread chronic diseases. It is the breakdown of the hard tissue in teeth caused by the common micro-organisms present in the oral cavity, mainly those adhered to the tooth in the form of bacterial plaque.Clinical studies have shown that strains such as L. rhamnosus SP 1 exhibits antagonistic activity against cariogenic bacteria, reduce the carie risk as well as a decrease in dental caries.

L. rhamnosus SP 1 intake reduces the caries increment in preschool children.

SP 1FOR CARIES DISEASE

Lactobacillus rhamnosus is the most studied probiotic bacterium among lactobacilli.

SURVIVAL DURINGGASTROINTESTINAL TRANSIT

Acid tolerance Bile tolerance

Simulated gastric juice Simulated pancreatic juice

95%

TECHNICAL INFORMATION

IDENTIFICATION16S rDNA gene sequence analysis (species characterization),PFGE (strain characterization)L. rhamnosus SP 1's PFGE profile is equivalent toL. rhamnosus LGG (ATCC53103)Also the complete genome is available

SAFETYincluded in EFSA QPS list (EFSA Journal 2017)absence of investigated antibiotic resistancegenes (EFSA Journal 2012; 10(6):2740)

BSE/TSE free

GMO free

Colorant free and hypoallergenic(list of allergens in compliance with Reg. UE 1169/2011)

STRAIN DEPOSIT NUMBERDSM 21690

STABILITY (Log10 cfu/g)

-20°C

20% reduction

1 log reduction

5°C

9,5

11,5

12,0

9,0

11,0

8,5

10,5

8,0

10,0

0 4010 20 30

Cel

l cou

nts

(Log

10 c

fu/g

)

Time (months)

SHELF LIFE18 months at temperature <-18°C12 months at temperature 5±3°C

CLINICAL TRIALRandomized, triple blind, placebo-controlled

Clinical dental examinations ICDAS criteria

RESULTS

70,00%

60,00%

50,00%

40,00%

30,00%

20,00%

10,00%

00,00%

caries prevalence develop of cavitated lesions

64,80%

54,40%

24,30%

9,70%

placebo group probiotic group

Sacco System is the new international biotech centre for innovation in nutraceutical and pharmaceutical products. Sacco System provides its customers with healthy, natural and functional products.We can offer a flexible service, develop formulation into adopted dosage forms, toll manufacturing (from laboratory to pilot production) and partnership for the development, production and the sale of single or mix probiotic strains of property or patented.

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REFERENCES:

Rodríguez G.., et al.; Probiotic Compared with Standard Milk for High-caries Children: A Cluster Randomized Trial. J Dent Res. 2016 Apr;95(4):402-7. doi: 10.1177/0022034515623935. Epub 2016 Jan 8.

10months

Daily dose

1,5x109

CFU/day

Children• 82 PLACEBO

•123 PROBIOTICS

205

Children age

2-3Age

The increment of dental caries showed an odds ratio of 0.35 (P<0.05) in favour of the probiotic group.

At the cavitated lesion level, the increment of new caries lesions within the groups showed 1.13 new lesions per child in the probiotic group compared with 1.75 lesions in the control group (P<0.05).

The probiotic group showed an increment of 0.58 ± 1.17 new lesions compared with 1.08 ± 1.70 new lesions observed in the control group.

The difference in caries increment wassignificant at the cavitated lesion level (P<0.01).

Interventionperiod