Kubelka munk absorbing layer stack theory
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Transcript of Kubelka munk absorbing layer stack theory
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Research Notes: Kubelka-Munk Absorbing Layer Stack TheoryApplying the Kubelka-Munk Absorbing Layer Stack Theory to Flower Coloration of the Chilean Bellflower, Nolana Paradoxa
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IntroductionResearchers from the University of Groningen, Netherlands are uniting the disciplines of biology and physics in the quantitative analysis of the reflectance spectra of flowers.
This has important implications for:
● Field of evolutionary biology● Understanding the relationship
between plants and pollinators● Understanding of optical
properties of pigmentation and light scattering
● Add to body of reference spectra data
● Industries such as thin film, coatings, dyes, and paints.
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Flower Petal AnatomyThe petals of a flower are made up of several layers consisting of outer epidermal layers and inner pigmented and light scattering layers of the mesophyll.
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Flower Anatomy (cont)Pigment layers selectively absorb incident light within a specific wavelength, while light scattering structures and vacuoles backscatter the incident light in all directions, giving the flower its color.
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Measuring the Colorization of FlowersDr. Doekle G. Stavenga studied the Chilean bellflower which has distinct color differentiation between the upper and lower surface
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Geometrical Optics for Flower ColorThe interaction of light within a plant to produce flower color used to be measured and explained by geometrical optics. However, plant structures are homogeneous, which makes optical analysis inconvenient.
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Kubelka-Munk TheoryTheory Outcomes:-Absorption and scattering coefficients to be derived from measured transmission and reflectance spectra-Stravenga and Van Der Kooi used this method on a non-invasive method for estimating chlorophyll content.
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Stacked Layer Theory-Used to describe flower colorationApplies Kubelka-Munk Theory -This method uses combined stack reflectance and transmission spectra to estimate the reflectance and transmission spectra for each layer
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Avantes Spectrophometric MeasurementResearchers employed the following
Avantes Spectrometers:
-(AvaLight-D(H)-S) to deliver light via optical fiber to an integrating sphere (AvaSphere-50-Refl).
-AvaSpec-ULS2048XL-USB2 Used to capture the reflected light by a second optical fiber.
Stavenga and van der Kooi then interpreted this measured transmission and reflectance spectra for the corolla using the Kubelka-Munk absorbing stack layer model
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Conclusion and Continuing Research-Casper van der Kooi and Doekele Stavenga has greatly advanced our understanding of how plants use light.
-Competition for pollinators affects the spectral display of flowers
-This research into the efficacy of the Kubelka-Munk method can have potentially wide-reaching impact on both the study of plants with potential agricultural applications, as well as potential relevance to other applications which concern stack metrology.