SFC

Kromasil SFCDesigned for green technology

Hichrom LimitedAuthorised DistributorTel: 0118 930 3660Fax: 0118 932 3484Email: sales@hichrom.co.uk

First choice in SFC separationsfor reliable and reproducible results

AkzoNobel, has been delivering Kromasil materials and columns for SFC for over 25 years.The new Kromasil SFC columns are based on the trusted, high performance, state-of-the-artspherical Kromasil silica particles, giving peace of mind to those working in the laboratory.

Kromasil SFC column choicesDesigned for fast separations and based on 2.5 µ mparticle size technology, the new Kromasil SFCcolumns are tailor-made for research, discovery andfor routine analysis. The new columns are deliveredin cyano , diol, silica, and 2-ethylpyridine chemistriesfor the laboratory scientist to separate and analyse awide range of substances, from non-polar to stronglypolar compounds.

The stationary phase quartet

ConditionsStationary phase: Kromasil SFC, 2.5 µ m

phase chemistry as in figureColumn size: 3.0 x 150 mmEluent: CO2 / methanol + 20 mM ammoniaGradient: 0 min: 5%,

10 min: 30% methanolFlow rate: 2 ml/minSubstances: 1 = alprenolol

2 = propranolol3 = acebutolol4 = pindolol

Temperature: 40° COutlet pressure: 120 barDetection: ES-MS and UV @ 220 nm

Separation of β-blockers

By using this standard set of Kromasil SFCcolumns, the user can efficiently screen thematerial that works best for a given sample.

[min]

12

3

42EP

012345[min]

1

2

3

4Diol

0246810

[min]

12

3

4SIL

036912

Fast separationsMedium and high-throughput laboratories working with green technology and seeking to improve turnaroundtime, are now able to take advantage of the separation power of the new Kromasil SFC 2.5 µ m family of columns.

Separation of steroids

With the chromatographic power ofKromasil SFC cyano phase users caneasely achieve baseline resolution within2.5 minutes of a generic linear gradient.

ConditionsColumn: Kromasil SFC, 2.5 µ m, CN, 3.0 x 150 mm Temperature: 40° CEluent: CO2 / methanol Outlet pressure: 120 barGradient: 0 min: 5%, 10 min: 30% methanol Detection: ES-MS and UV @ 220 nmFlow rate: 2 ml/minSubstances: 1 = deoxycorticosterone, 2 = corticosterone, 3 = cortisone, 4 = hydrocortisone

[min]

12

3

4

024

[min]

1

2

3

0246

Separation of anticonvulsants

Due to the outstanding properties ofKromasil silica, analysts can now achievebaseline resolution for anticonvulsantsusing a generic gradient, resulting insharp and Gaussian peaks.

ConditionsColumn: Kromasil SFC, 2.5 µ m, SIL, 3.0 x 150 mm Temperature: 40° CEluent: CO2 / methanol Outlet pressure: 120 barGradient: 0 min: 5%, 10 min: 30% methanol Detection: ES-MS and UV @ 220 nmFlow rate: 2 ml/min Substances: 1 = phenytoin, 2 = carbamazepine, 3 = primidone

www.kromasil.com

The moment you adopt our Kromasil High Performance Concept, youjoin thousands of chromatographers who share a common goal: toachieve better separations when analyzing or isolating pharmaceu-ticals or other substances.

Not only will you benefit from our patented silica technology, but yougain a strong partner with a reliable track record in the field of silicaproducts. For the past 70 years, we have pioneered new types ofsilica. Our long experience in the field of silica chemistry is thesecret behind the development of Kromasil, and the success of ourSeparation Products group. Kromasil is available in bulk and in high-pressure slurry-packed columns. The development, production andmarketing of Kromasil are ISO 9001 certified.

Kromasil is a brand of AkzoNobel, a leading global paint andcoatings company and a major producer of specialty chemicals withheadquarters in Amsterdam, the Netherlands. With 48 000 people inmore than 80 countries around the world, we are committed tosustainability and delivering leading products and technologies tomeet the growing demands of our fast-changing planet, making lifemore liveable and our cities more human.

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Kromasil® is a registered trademark of AkzoNobelin a number of territories in the world.

AvailabilityWith more than 25 years of stationary phasemanufacturing and packing expertise, AkzoNobelcontinues to deliver performance products tocustomers in the pharmaceutical, food and beverage,clinical and environmental industries.

The Kromasil SFC columns are available in 2.5 µ mparticle size with the following surface chemistries:

• silica (SIL)• cyano (CN)• diol (Diol)• 2-ethylpyridine (2EP)

See table to the right for actual part numbers.

Separation of anti-inflammatory drugs

With its endcapping and aromaticproperties, Kromasil SFC with2-ethylpyridine offers a unique separationpower that makes it standout from the rest.

ConditionsColumn: Kromasil SFC, 2.5 µ m, 2EP, 3.0 x 150 mm Temperature: 40° CEluent: CO2 / methanol Outlet pressure: 120 barGradient: 0 min: 5%, 10 min: 30% methanol Detection: ES-MS and UV @ 220 nmFlow rate: 2 ml/minSubstances: 1 = ibuprofen, 2 = fenoprofen, 3 = flurbiprofen, 4 = ketoprofen

[min]

1

23 4

01234

Alternative selectivity

Columnsize(I.D.xlength)

Material 3.0x150mm 4.6x150mm

KromasilSFC-2.5-SIL FH2SIC15 FH2SIA15

KromasilSFC-2.5-CN FH2CNC15 FH2CNA15

KromasilSFC-2.5-Diol FH2DIC15 FH2DIA15

KromasilSFC-2.5-2EP FH2EPC15 FH2EPA15

For further details contact:Hichrom Limited, 1 The Markham Centre, Station Road, Theale, Reading, Berkshire, RG7 4PETel: 0118 930 3660 Fax: 0118 932 3484 Email: technical@hichrom.co.uk, www.hichrom.co.uk