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Klebsiella oxytoca and K1 enzyme (indole positive) Chromosomal R/ ampicillin Mutational...
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Transcript of Klebsiella oxytoca and K1 enzyme (indole positive) Chromosomal R/ ampicillin Mutational...
![Page 1: Klebsiella oxytoca and K1 enzyme (indole positive) Chromosomal R/ ampicillin Mutational hyperproduction R/ cephalosporins Less susceptible to clavulanic.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649d485503460f94a2375b/html5/thumbnails/1.jpg)
Klebsiella oxytocaand K1 enzyme
(indole positive)
• Chromosomal
R/ ampicillin • Mutational hyperproduction
R/ cephalosporins• Less susceptible to clavulanic acid than ESBL
No key holeKey hole not obvious
![Page 2: Klebsiella oxytoca and K1 enzyme (indole positive) Chromosomal R/ ampicillin Mutational hyperproduction R/ cephalosporins Less susceptible to clavulanic.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649d485503460f94a2375b/html5/thumbnails/2.jpg)
A Klebsiella oxytoca with basal K1 -lactamase resistant to ampicillin and susceptible to Augmentin, cephalexin and cefotaxime.
![Page 3: Klebsiella oxytoca and K1 enzyme (indole positive) Chromosomal R/ ampicillin Mutational hyperproduction R/ cephalosporins Less susceptible to clavulanic.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649d485503460f94a2375b/html5/thumbnails/3.jpg)
A hyperproducer Klebsiella oxytoca with a high level of mutational K1 -lactamase shows resistance to Augmentin, cephalexin and cefotaxime.
![Page 4: Klebsiella oxytoca and K1 enzyme (indole positive) Chromosomal R/ ampicillin Mutational hyperproduction R/ cephalosporins Less susceptible to clavulanic.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649d485503460f94a2375b/html5/thumbnails/4.jpg)
Another hyperproducer Klebsiella oxytoca with a high level of mutational K1 -lactamase. Note the tiny "keyhole" between cefotaxime (CTX 5) and Augmentin (AMC 60) and the small inhibitory zone around cephalexin (CL100). The tiny "keyhole“ is a “bonus” as most strains with high K1 activity simply show resistance to Augmentin, cephalexin and cefotaxime.
![Page 5: Klebsiella oxytoca and K1 enzyme (indole positive) Chromosomal R/ ampicillin Mutational hyperproduction R/ cephalosporins Less susceptible to clavulanic.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649d485503460f94a2375b/html5/thumbnails/5.jpg)
A hyperproducer Klebsiella oxytoca with a low level of mutational K1 -lactamase. Note the slight synergy between Augmentin (AMC 60) and the cephalosporin discs cephalexin (CL100) and cefotaxime (CTX 5).
![Page 6: Klebsiella oxytoca and K1 enzyme (indole positive) Chromosomal R/ ampicillin Mutational hyperproduction R/ cephalosporins Less susceptible to clavulanic.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649d485503460f94a2375b/html5/thumbnails/6.jpg)
A Klebsiella oxytoca with an ESBL. It’s likely that the isolate only has basal K1 -lactamase (large zone around AMC 60).
![Page 7: Klebsiella oxytoca and K1 enzyme (indole positive) Chromosomal R/ ampicillin Mutational hyperproduction R/ cephalosporins Less susceptible to clavulanic.](https://reader036.fdocuments.in/reader036/viewer/2022062516/56649d485503460f94a2375b/html5/thumbnails/7.jpg)
Reporting
Klebsiella oxytoca hyperproducer of K1
Not ESBL (plasmid mediated)
As per Table 4
R/ ampicillin, cephalexin, cefotaxime…..