Kinetex 1.7 µm UHPLC Columns - Chromservis...• Simple to use • Extend column lifetime •...

Core-Shell Technology

The chosen core-shell brand to drive UHPLC performance to the next level

Kinetex 1.7 µm UHPLC Columns



Simply AdvantageousThe band broadening (wide peaks) and lengthy retention times of traditional fully porous products can limit your

results. Now you can use the incredible efficiency levels of Kinetex Core-Shell Technology to achieve shorter run times, higher levels of sensitivity, and overall better UHPLC results.

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AP

P ID

228

05

Kinetex Core-Shell

Traditional Fully Porous

Get faster, better results

with me!

Kinetex Core-Shell

Traditional Fully Porous

Phenomenex l WEB: www.phenomenex.com


Upgrade the efficiency and performance of your current traditional sub-2 μm column with a Kinetex 1.7 μm core-shell column. Extra resolution, higher peak capacities, and greater sensitivity are all within your reach with Kinetex Core-Shell Technology.

The First 1.7 µm Core-Shell Column

Waters® ACQUITY® BEH 1.7 µm C18

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05

Kinetex 1.7 µm XB-C18

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Higher Resolution(between peaks 1 and 2)



66% 138% 21%

Conditions for both columns:Column: Kinetex 1.7 µm XB-C18

ACQUITY BEH 1.7 µm C18

Dimensions: 150 x 2.1 mm

Mobile Phase: A: Milli-Q® Water B: Acetonitrile

Gradient: Time (min) 05

% B30100

Flow Rate: 0.5 mL/min

Temperature: Ambient

Detection: UV @ 210 nm (ambient)

Backpressure: 891 bar (Kinetex)935 bar (ACQUITY)

Instrument: Waters ACQUITY UPLC®

Sample: 1. Hydrocortisone2. Cortisone3. Corticosterone4. Cortisone-21-Acetate5. DHEA (Dehydroepiandrosterone)6. 17-Hydroxyprogesterone7. Deoxycorticosterone8. Progesterone

Comparative separations may not be representative of all applications.



The increased efficiency of Kinetex 1.7 μm allows you to use shorter columns for faster run times while keeping optimal performance. Now it’s time for you to challenge your difficult separations with the superior efficiency and resolving power of a Kinetex 1.7 μm core-shell column.

Superior and Faster Resolving Power

Inte

nsity

, cp

s

Fully Porous 3.5 µm C18

0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 min0

5500

Inte

nsity

, cp

s

Fused-Core® 2.7 µm C18

0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 min0

6000

1.15e4

Inte

nsity

, cp

s

Kinetex 1.7 µm C18 Faster analysis, better sensitivity, and overall

better resolution!

0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 min0

7000

1.4e4

AP

P ID

206

86A

PP

ID 2

0686

AP

P ID

206

86

Conditions for all columns:Columns: Fully porous 3.5 µm C18 (50 x 2.1 mm)

Fused-Core 2.7 µm C18 (50 x 2.1 mm) Kinetex 1.7 µm C18 (30 x 2.1 mm)

Mobile Phase: A: 10 mM Ammonium Formate B: Acetonitrile


2.1

% B5

1005


Temperature: 22 °C

Detection: MS/MS (SCIEX API 4000™)

Sample: Lorazepam glucuronide




Highly sensitive UHPLC separations are dependent upon consistent quantitation and consistent results. With the reliability of the Kinetex columns and media batches, you no longer have to settle for the inconsistent results of inferior products that exist on the market. Our highest standards of quality will ensure that you are fully satisfied with each and every Kinetex column!

Trust / Consistency

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Batch 13

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Batch 12

Conditions same for all batches:Column: Kinetex 1.7 µm F5

Dimensions: 50 x 4.6 mmMobile Phase: A: 0.1 % Formic Acid in Water

B: 0.1 % Formic Acid in AcetonitrileGradient: 5-95 % B over 5 min

Flow Rate: 1.85 mL/minTemperature: 30 ˚C

Detection: UV @ 254 nm (ambient)Sample: 1. Uracil

2. Pindolol3. Chlorpheniramine4. Nortriptyline5. 3-Methyl-4-Nitrobenzoic acid6. 5-Methyl Salicyl Aldehyde7. Hexaphenone




By upgrading from fully porous hybrid UHPLC columns to the Kinetex 1.7 μm EVO C18, you can keep the pH stability but gain much greater performance. With the core-shell advantage at your disposal your new methods will be faster and more sensitive, leaving room for greater productivity.

Upgrade from Hybrid Materials

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mAU

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Conditions for both columns:Column: Kinetex 1.7 µm EVO C18

ACQUITY BEH 1.7 µm C18


Mobile Phase: A: 0.1 % Formic Acid in WaterB: 0.1 % Formic Acid in Methanol


% B295


Temperature: 30 º C


Sample: 1. Berberine

Berberine Standard

AP

P ID

231

11 a

nd 2

3109Greater Resolution

of Impurities

Waters® ACQUITY® BEH 1.7 µm C18

Kinetex 1.7 µm EVO C18

57%Increase in Peak Height Response




Kinetex columns are compatible with ALL UHPLC Instruments.

Kinetex on Agilent® 1290

Kinetex on Shimadzu® Nexera®

Kinetex on JASCO® X-LC

Kinetex on Waters® ACQUITY UPLC®

UHPLC Compatible

Need help installing your Kinetex column? www.phenomenex.com/kinetex/technicalresources



With the combination of the novel Kinetex EVO C18, F5, and Biphenyl selectivities at your disposal, reversed phase UHPLC method development can be taken to the next level. With its exceptional peak shape for basic compounds, the EVO C18 is a dynamic tool that gives core-shell high performance while allowing retention to be enhanced by adjusting the pH of the mobile phase.

Utilizing Powerful Selectivities

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pH 2.5

pH 10

Utilize High pH Stability of EVO C18 to Enhance Retention

Move to Orthogonal Solutions!

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P ID

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82

Conditions for all columns same except where noted:Column: Kinetex 1.7 µm EVO C18


Part No.: 00B-4726-AN

Mobile Phase: A: 20 mM Potassium phosphate (pH 2.5)B: Methanol

Mobile Phase: A: 20 mM Potassium phosphate (pH 10)B: Methanol


% B590




Sample: 1. Fluvoxamine

EVOC18

TMSTMS

EVOC18

TMSTMS



Next to the EVO C18, the orthogonal Kinetex F5 and Biphenyl will drive enhancement of reversed phase methods with their versatile array of interaction mechanisms. Varying degrees of electrostatic, aromatic, reversed phase, steric, and hydrogen bonding between the F5 and Biphenyl mean that these two stationary phases are both excellent alternatives to a C18. Additionally the F5 is known for its ability to separate isomeric species, while the pi electron cloud created by the dual ring of the Biphenyl phase acts like a weak negative charge and aids basic compound retention.

Utilizing Powerful Selectivities

Kinetex 1.7 µm F5

Move to Orthogonal Solutions!

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Kinetex 1.7 µm Biphenyl

pH 2.5

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F

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TMSTMS

TMSTMS

Biphenyl

Conditions for all columns:Column: Kinetex 1.7 µm F5

Kinetex 1.7 µm Biphenyl


Part No.: Kinetex F5: 00B-4722-ANKinetex Biphenyl: 00B-4628-AN

Mobile Phase: A: 20 mM Potassium phosphate (pH 2.5)B: Methanol


% B590




Sample: 1. Fluvoxamine

AP

P ID

230

81A

PP

ID 2

3080



Protect your column, including Kinetex core-shell columns, from damaging contaminants and microparticulates with the SecurityGuard ULTRA guard cartridge system!

• Simple to use

• Extend column lifetime

• Pressure rated to 20,000 psi (1,378 bar)

• Fits virtually all manufacturers’ columns 2.1 to 4.6 mm ID

UHPLC Column Protection

SecurityG

uard™ U

LTRA

UH

PLC

Colum

n to Detector

From Injection

CartridgeHolder

Material Chamber

Frit

}Compounds of Interest

C o n t a m i n a n t s and Particulates

See it in action: www.phenomenex.com/SecurityGuardULTRA

SecurityGuard ULTRA Guard Cartridge System (Cartridge connected to Holder)

Protect any UHPLC Column



Kinetex Phase SelectionPhase Best Use pH Stability

EVO C18 Robust reversed phase methods even in alkaline conditions with improved peak shape for polar basic compounds 1 - 12

C18 All purpose phase that offers the hydrophobic retention and methylene selectivity chromatographers expect from a C18 column 1.5 - 8.5*

XB-C18 C18 phase with protective butyl side chains for improved peak shape for basic compounds under neutral and acidic conditions 1.5 - 8.5*

C8 USP L7 phase that provides less hydrophobic and methylene selectivity than a C18 1.5 - 8.5*

F5 Highly reproducible pentafluorophenyl propyl phase that offers a unique combination of polar, hydrophobic, aromatic, and shape selectivity 1.5 - 8.5

Biphenyl 100% aqueous stable and allows for excellent reversed phase retention and enhanced polar and aromatic selectivity 1.5 - 8.5*

Phenyl-Hexyl Reversed phase chemistry that allows for greater retention and separation of aromatic hydrocarbons 1.5 - 8.5*

HILIC Unbonded silica phase for HILIC conditions to provide selectivity for polar compounds 2.0 - 7.5

*pH stability under gradient conditions. pH stability is 1.5-10 under isocratic conditions.


If you are not completely satisfied with Kinetex core-shell columns, send in your comparative data to a similar product within 45 days and keep the column for FREE.

Ordering Information

1.7 μm Minibore Columns (mm)SecurityGuard™

ULTRA Cartridges‡

Phases 30 x 2.1 50 x 2.1 100 x 2.1 150 x 2.1 3/pk

EVO C18 –– 00B-4726-AN 00D-4726-AN 00F-4726-AN AJ0-9298

F5 –– 00B-4722-AN 00D-4722-AN 00F-4722-AN AJ0-9322Biphenyl –– 00B-4628-AN 00D-4628-AN 00F-4628-AN AJ0-9209XB-C18 00A-4498-AN 00B-4498-AN 00D-4498-AN 00F-4498-AN AJ0-8782C18 00A-4475-AN 00B-4475-AN 00D-4475-AN 00F-4475-AN AJ0-8782C8 00A-4499-AN 00B-4499-AN 00D-4499-AN 00F-4499-AN AJ0-8784HILIC 00A-4474-AN 00B-4474-AN 00D-4474-AN –– AJ0-8786Phenyl-Hexyl –– 00B-4500-AN 00D-4500-AN 00F-4500-AN AJ0-8788

for 2.1 mm ID‡ SecurityGuard ULTRA Cartridges require holder, Part No.: AJ0-9000

Terms and Conditions Subject to Phenomenex Standard Terms & Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions.Trademarks Kinetex is a registered trademark and SecurityGuard is a trademark of Phenomenex. Waters, ACQUITY, and UPLC are registered trademarks, and BEH Technology is a trademark of Waters Corporation. Agilent is a registered trademark of Agilent Technologies, Inc. Shimadzu and Nexera are registered trademarks of Shimadzu Corporation. JASCO is a registered trademark of JASCO, Corporation. Fused-Core is a registered trademark of Advanced Materials Technology, Inc. API 4000 is a trademark of AB SCIEX Pte. Ltd. AB SCIEX is being used under license. Milli-Q is a registered trademark of Merck KGaA, Darmstadt, Germany.Disclaimer Phenomenex is not affiliated with Advanced Materials Technology, Agilent Technologies, JASCO, or Waters Corporation. Comparative separations may not be representative of all applications.Kinetex EVO is patented by Phenomenex. U.S. Patent Nos. 7,563,367 and 8,658,038 and foreign counterparts. © 2016 Phenomenex, Inc. All rights reserved.

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t: +1 (310) 212-0555f: +1 (310) 328-7768

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BR

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Kinetex 1.7 µm UHPLC Columns - Chromservis...• Simple to use • Extend column lifetime •...

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