KINESIN. Microtubules Questions How much ATP is hydrolysed per step? How many rate-limiting steps...

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KINESIN

Transcript of KINESIN. Microtubules Questions How much ATP is hydrolysed per step? How many rate-limiting steps...

Page 1: KINESIN. Microtubules Questions How much ATP is hydrolysed per step? How many rate-limiting steps are there? What is the mechanism of movement? Analyse.

KINESIN

Page 2: KINESIN. Microtubules Questions How much ATP is hydrolysed per step? How many rate-limiting steps are there? What is the mechanism of movement? Analyse.

Microtubules

Page 3: KINESIN. Microtubules Questions How much ATP is hydrolysed per step? How many rate-limiting steps are there? What is the mechanism of movement? Analyse.

Questions

• How much ATP is hydrolysed per step?

• How many rate-limiting steps are there?

• What is the mechanism of movement?

Analyse movement

Simulation of randomness

Page 4: KINESIN. Microtubules Questions How much ATP is hydrolysed per step? How many rate-limiting steps are there? What is the mechanism of movement? Analyse.

Michaelis-Menten kinetics

v = kcat [ATP]/(Km + [ATP])

When [ATP] is limmiting: v = [ATP] kcat/Km – linear function

Km: Michaelis constant: concentration of ATP at ½vmax

kcat: Velocity at saturatet [ATP]

Kinesin:Km = 62 +/- 8 µMkcat = 680 +/- 31 nm/s

At limiting [ATP]: kcat/Km = 11 +/- 1 nm s-1 µM-1

Page 5: KINESIN. Microtubules Questions How much ATP is hydrolysed per step? How many rate-limiting steps are there? What is the mechanism of movement? Analyse.
Page 6: KINESIN. Microtubules Questions How much ATP is hydrolysed per step? How many rate-limiting steps are there? What is the mechanism of movement? Analyse.
Page 7: KINESIN. Microtubules Questions How much ATP is hydrolysed per step? How many rate-limiting steps are there? What is the mechanism of movement? Analyse.
Page 8: KINESIN. Microtubules Questions How much ATP is hydrolysed per step? How many rate-limiting steps are there? What is the mechanism of movement? Analyse.

Randomness parameter

d = step distancex(t) = position

r = 0: Clock-liker = 1: 1 rate-limiting step0 < r < 1: more ratelimiting stepsr > 1: increasing variance (backwards steps, double steps, biochemically inactivatet states)

Page 9: KINESIN. Microtubules Questions How much ATP is hydrolysed per step? How many rate-limiting steps are there? What is the mechanism of movement? Analyse.
Page 10: KINESIN. Microtubules Questions How much ATP is hydrolysed per step? How many rate-limiting steps are there? What is the mechanism of movement? Analyse.
Page 11: KINESIN. Microtubules Questions How much ATP is hydrolysed per step? How many rate-limiting steps are there? What is the mechanism of movement? Analyse.

A small-molecule activator of kinesin-1 drives remodeling ... · light chains (KLCs), which contain binding sites for both organelle-specific cargo adaptors and microtubules (9, 11

A small-molecule activator of kinesin-1 drives remodeling ... · light chains (KLCs), which contain binding sites for both organelle-specific cargo adaptors and microtubules (9, 11

Vesicle transport on microtubules

Vesicle transport on microtubules

High Cell Density Conversion of Hydrolysed Waste Cooking ...

High Cell Density Conversion of Hydrolysed Waste Cooking ...

The Loop 5 Element Structurally and Kinetically ... · ABSTRACT Eg5 is a homotetrameric kinesin-5 motor protein that generates outward force on the overlapping, antiparallel microtubules

The Loop 5 Element Structurally and Kinetically ... · ABSTRACT Eg5 is a homotetrameric kinesin-5 motor protein that generates outward force on the overlapping, antiparallel microtubules

Lecture 7: the cytoskeleton and cell movement …...Microtubules-motor proteins such as kinesin and dynein move along microtubules in opposite directions kinesins move toward the plus

Lecture 7: the cytoskeleton and cell movement …...Microtubules-motor proteins such as kinesin and dynein move along microtubules in opposite directions kinesins move toward the plus

I. INTRODUCTION - arXiv · kinesin walking along microtubules and ribosomes moving along mRNA [2, 3]. These laments act as one-dimensional lanes that allow proteins to move over long

I. INTRODUCTION - arXiv · kinesin walking along microtubules and ribosomes moving along mRNA [2, 3]. These laments act as one-dimensional lanes that allow proteins to move over long

Molecular crowding creates traffic jams of kinesin motors ... · Molecular crowding creates traffic jams of kinesin motors on microtubules Cécile Leduca,b,1, Kathrin Padberg-Gehlec,1,

Molecular crowding creates traffic jams of kinesin motors ... · Molecular crowding creates traffic jams of kinesin motors on microtubules Cécile Leduca,b,1, Kathrin Padberg-Gehlec,1,

High-resolution structures of kinesin on microtubules ... · High-resolution structures of kinesin on microtubules provide a basis for nucleotide-gated force-generation Zhiguo Shang1†,

High-resolution structures of kinesin on microtubules ... · High-resolution structures of kinesin on microtubules provide a basis for nucleotide-gated force-generation Zhiguo Shang1†,

Superresolution and pulse-chase imaging ... - Science Advances · transported along microtubules by kinesin-1 and further along actin filaments by myosin-5 to the hyphal tip for exocytosis

Superresolution and pulse-chase imaging ... - Science Advances · transported along microtubules by kinesin-1 and further along actin filaments by myosin-5 to the hyphal tip for exocytosis

Microtubules - Fibonacci

Microtubules - Fibonacci

Native kinesin‐1 does not bind preferentially to GTP‐tubulin‐rich microtubules …xulab.ucmerced.edu/uploads/6/6/2/2/66221941/li_et_al... · 2019. 10. 25. · Molecular motor-based

Native kinesin‐1 does not bind preferentially to GTP‐tubulin‐rich microtubules …xulab.ucmerced.edu/uploads/6/6/2/2/66221941/li_et_al... · 2019. 10. 25. · Molecular motor-based

Kinesin-2motors:Kineticsandbiophysics...kinesin-3, kinesin-5 Eg5/KSP, and kinesin-7 CENP-E, and all are best known for their roles in intracellular transport. N-kinesins carry cargo

Kinesin-2motors:Kineticsandbiophysics...kinesin-3, kinesin-5 Eg5/KSP, and kinesin-7 CENP-E, and all are best known for their roles in intracellular transport. N-kinesins carry cargo

HEAT-INDUCED CHANGES IN LACTOSE HYDROLYSED MILKS …lib.tkk.fi/Diss/2008/isbn9789512293995/isbn9789512293995.pdf · Heat-induced changes in lactose hydrolysed ... and was found to

HEAT-INDUCED CHANGES IN LACTOSE HYDROLYSED MILKS …lib.tkk.fi/Diss/2008/isbn9789512293995/isbn9789512293995.pdf · Heat-induced changes in lactose hydrolysed ... and was found to

Kinesin rotates unidirectionally and generates torque ... · Kinesin rotates unidirectionally and generates torque while walking on microtubules Avin Ramaiyaa,1, Basudev Roya,1,2,

Kinesin rotates unidirectionally and generates torque ... · Kinesin rotates unidirectionally and generates torque while walking on microtubules Avin Ramaiyaa,1, Basudev Roya,1,2,

Microtubules and Guidance

Microtubules and Guidance

Engineering Oscillating Microtubule Bundles...bundles are assembled in mixtures comprising microtubules (MTs), kinesin clusters, and a depletion agent which holds the MTs together

Engineering Oscillating Microtubule Bundles...bundles are assembled in mixtures comprising microtubules (MTs), kinesin clusters, and a depletion agent which holds the MTs together

Microtubules Structure and Function

Microtubules Structure and Function

Moisture sorption and stickiness behaviour of hydrolysed ...

Moisture sorption and stickiness behaviour of hydrolysed ...

The Kinesin-like Calmodulin Binding Protein Is ...et al., 1998; Narasimhulu and Reddy, 1998). Ca 2 1-CaM therefore negatively regulates the interaction of KCBP with microtubules. Here,

The Kinesin-like Calmodulin Binding Protein Is ...et al., 1998; Narasimhulu and Reddy, 1998). Ca 2 1-CaM therefore negatively regulates the interaction of KCBP with microtubules. Here,

Drosophila PAT1 is required for Kinesin-1 to transport ...of Kinesin are reduced in cell extracts that lack PAT1, suggesting that the requirement for PAT1 during Kinesin-1-mediated

Drosophila PAT1 is required for Kinesin-1 to transport ...of Kinesin are reduced in cell extracts that lack PAT1, suggesting that the requirement for PAT1 during Kinesin-1-mediated