Keystone review 1
description
Transcript of Keystone review 1
![Page 1: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/1.jpg)
KEYSTONE REVIEW 1Biochemistry through DNA/protein synthesis
![Page 2: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/2.jpg)
Biochemistry• What is an atom?
• An atom is the most basic unit of structure. Each element on the period table, such as carbon, hydrogen and oxygen, are made up of atoms.
• Atoms consist of three particles• Protons: have a positive charge• Electrons: have a negative charge • Neutrons: have a neutral charge
• What are compounds?• Compounds form when atoms join together, like water, which is
hydrogen atoms and oxygen atoms bonded together.
![Page 3: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/3.jpg)
Biochemistry• What is a bond?
• The sharing or transferring of electrons in the atom’s outer “shell”• Sharing electrons: covalent bond• Transferring electrons: ionic bond• Hydrogen bonds from between hydrogen atoms and other atoms
![Page 4: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/4.jpg)
Biochemistry• What’s so special about carbon?
• Carbon has 4 electrons in it’s outer shell so it can form 4 bonds• Carbon is found in all of the macromolecules such as
• Proteins• Lipids (fats)• Carbohydrates• Nucleic acids (DNA material)
![Page 5: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/5.jpg)
Biochemistry• What’s so special about water?
• Water (hydrogen atoms + oxygen atoms) is a polar molecule• Polarity refers to the distribution of electrons between two atoms (that
are sharing their electrons)• Water is considered polar because the hydrogen atom’s electrons are
shared UNEQUALLY with the oxygen atom’s electrons
• Polar Nonpolar
![Page 6: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/6.jpg)
Biochemistry• What’s so special about water?
• It’s attracted to some substances and it repels others• Hydrophobic: water-hating. These substances don’t mix with water
• Ex: Oil• Hydrophilic: water-loving. These substances DO mix and dissolve
in water• Ex: Sugar
![Page 7: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/7.jpg)
Biochemistry• What’s so special about water?
• Water is a natural buffer, meaning it stabilizes the pH of substances• Acid: any substance that releases hydrogen ions (H+) when
dissolved in water• Ex: lemon juice, hydrochloric acid
• Base: any substance that releases hydroxide ions (OH-) when dissolved in water• Ex: ammonia, bleach
![Page 8: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/8.jpg)
Biochemistry• What’s so special about water?
• Water molecules have sticky properties• Adhesion: when water sticks to other surfaces
• Think: water sticking to your car windshield• Cohesion: when water molecules stick to one another
• Think; water forming droplets • Surface tension: when water forms a surface that resists external
forces• Think: belly flopping off the diving board
• Capillary action: when water flows against gravity up a plant stem• Think: paper towel soaking up water
![Page 9: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/9.jpg)
Biochemistry• Macromolecules
• Proteins• Lipids• Carbohydrates• Nucleic acids
• Made up of monomers (building blocks)• Macromolecules build together to make cell structures,
which build together to make us!
![Page 10: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/10.jpg)
Biochemistry• Proteins
• Monomer: amino acid• Elements that make up proteins: carbon (C), nitrogen (N), oxygen
(O), hydrogen (H) and sometimes sulfur (S)• Function/propose: make up muscles, act as markers on cells
(identify the cell as a special type), act as enzymes that speed up reactions, fight disease and transport materials into and out of cells
• Reaction: amino acids are joined together through a reaction called dehydration synthesis. Two amino acids are joined together by a bond called a peptide bond
![Page 11: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/11.jpg)
Biochemistry•Amino acids are made up of a central carbon that is attached to a carboxyl group (COOH), a hydrogen, an amino group (NH2) and a “R” group•R groups differ from one amino acid to another, making each amino acid unique•There are 20 amino acids in existence
Alanine Serine
![Page 12: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/12.jpg)
Biochemistry• When amino acids join together (through a reaction called
dehydration synthesis, and by a bond called a peptide bond), they from a polypeptide
• A polypeptide is a chain of amino acids• Polypeptides=proteins
![Page 13: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/13.jpg)
Biochemistry• Enzymes are types of proteins. Their job is to speed up
reactions and act as catalysts. • Enzymes fit, like a lock and key, with a substrate. The place
where they join is called the activation site.• A substrate is what the enzymes acts on.• EX: lactose intolerance
• Lactose is a sugar found in dairy products. Normally, there is an enzyme that occurs naturally in a person’s body. When they consume dairy products, and their body is filled with the lactose sugar, the enzyme lactase acts on the lactose sugar and breaks it down so it can be digested. People with lactose intolerance are lacking the enzyme (or it doesn’t function properly) lactase, so when they eat dairy products and take in the lactose sugar, they are unable to digest it and they have stomach “issues” as a result.
![Page 14: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/14.jpg)
Biochemistry• This is not the only function of enzymes. • Enzymes also speed up reactions so that your body
doesn’t have to use all of it’s energy (that it gets from breaking down food) on simple tasks like breathing.
• Enzymes are never used up, but can be affected by things like pH, temperature and salt concentration. These things can alter an enzyme’s shape, making it impossible for the substrate and the enzyme to fit properly.
![Page 15: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/15.jpg)
![Page 16: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/16.jpg)
Biochemistry• Lipids are fats, oils, steroids, and waxes
• Monomer: Fatty acid• Elements: Carbon (C), hydrogen (H), Oxygen (O). They have HIGH
amount of hydrogen atoms (for every 1 carbon there are 2 hydrogens)
• Function/purpose: Fats provide cells with protection and insulation, steroids are our hormones, waxes provide waterproof coverings (ear wax or waxy plant leaves like ivy). Lipids make up the cell membrane (lipid bilayer!)
![Page 17: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/17.jpg)
Biochemistry• Different types of lipids
• Saturated: fats that have single (covalent) bonds between the carbon atoms
• Unsaturated: fats that have some double (covalent) bonds between the carbon atoms. The electrons are shared twice, in a sense.
• Polyunsaturated: fat that have many double (covalent) bonds between the carbon atoms.
![Page 18: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/18.jpg)
![Page 19: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/19.jpg)
Biochemistry• Carbohydrates
• Monomer: monosaccharide• Elements: Carbon (C), Hydrogen (H) and Oxygen (O)• Function/propose: main source of energy for the body• Reaction: Dehydration synthesis joins two monosaccharides
together.
![Page 20: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/20.jpg)
Biochemistry• Disaccharides: two monosaccharides joined together• Polysaccharides: many monosaccharides joined together
![Page 21: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/21.jpg)
Biochemistry• Nucleic acids
• Monomer: nucleotide• Elements: Carbon (C), Oxygen (O), Nitrogen (N), Phosphorus (P),
and Hydrogen (H)• Base: Adenine, thymine, cytosine, and guanine• Sugar: a monosaccharide• Phosphate bonded to 3 hydrogens
• Function/purpose: DNA (the heredity molecule that stores genetic information, RNA (like DNA’s simpler form), ATP and NAD (energy storing molecules involved in photosynthesis and cellular respiration reactions)
![Page 22: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/22.jpg)
Biochemistry• Two types of bases: pyrimidine and purines. • Adenine (A) and guanine (G) are purines• Thymine (T) and cytosine (C) are pyrimidine
![Page 23: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/23.jpg)
Cells• The cell membrane
• Function: to control what enters and exits the cell• Composition: a phospholipid bilayer (two layers of fats)
• Arranged tail to tail
![Page 24: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/24.jpg)
Cells• Also embedded in the cell membrane are
• Proteins: help large molecules move into and out of the cell • Peripheral protein: on the surface of the cell (mostly involved in cell
identification and recognition)• Integral protein: go the entire way through the membrane (helps
molecules get into and out of the membrane, like a tunnel!)• Cholesterol: makes the membrane more rigid
![Page 25: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/25.jpg)
Cells• Transport into and out of the cell
• Passive transport: doesn’t require energy• Molecules move by diffusion: the movement of molecules from a high
concentration to a low concentration• THINK: does it take energy to ride a bike from high on a mountain to low
on a mountain?• Solute: the solid substance being dissolved (sugar, salt etc.)• Solvent: the liquid substance doing the dissolving (usually water)
![Page 26: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/26.jpg)
Cells• Diffusion occurs when a “system” is not a equilibrium• Equilibrium is when all things are equal• Solutes move from high low until there is an equal
amount on each side (of the cell, inside/out)• Things that affect diffusion
• Temperature: a system at a higher temperature will cause diffusion to occur more quickly. THINK: does hot chocolate powder dissolve faster in hot water or cold water?
• Size: larger solute molecules diffuse slower into/out of a system or a cell. THINK: human running through a jell-o wall vs. an ant
![Page 27: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/27.jpg)
Cells• Osmosis is the diffusion of specifically WATER into or out
of a cell• Cells need water to live but too much water can be fatal• Types of tonicity
• Hypertonic solutions: cause the cell to shrink because water is leaving the cell. • Why is water leaving? The concentration of water inside the cell is
greater than the concentration of water outside of the cell, so the water moves from high to low OUT OF THE CELL
• Hypotonic solutions: cause the cell to swell because water is entering the cell.• Why is water entering? The concentration of water inside the cell is
lesser than the concentration of water outside the cell, so the water moves from high to low INTO THE CELL
![Page 28: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/28.jpg)
Cells• Isotonic solution: water moves into and out of the cell at an equal
rate
Cytosol is the gel-like fluid inside a cell (in this case, a red blood cell)
![Page 29: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/29.jpg)
Cells• Facilitated diffusion: molecules that need help crossing
the membrane use proteins as tunnels. They still move from a high concentration to a low concentration.
Glucose
Phospholipid bilayer
Integral protein
High concentration
Low concentration
![Page 30: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/30.jpg)
HighConcentration
LowConcentration
CellMembrane
Glucosemolecules
Proteinchannel
![Page 31: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/31.jpg)
Cells• Active transport: requires energy to move molecules from
a low concentration to a high concentration• THINK: does it require energy to move a bike from low on a
mountain to high on a mountain?• Pumps are required to move the molecules
![Page 32: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/32.jpg)
Cells
•Endocytosis - cell membrane engulfs and takes in materials •Phagocytosis - solid material taken in
•Pinocytosis - liquid material take in
![Page 33: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/33.jpg)
Cells• Vesicles, sacs that are carrying either solid or liquid
materials, fuse with the cell membrane and release their contents or take in contents
![Page 34: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/34.jpg)
Cells• Types of cells
• Prokaryotic cells: cells that lack a nucleus and other internal organelles. • Ex: bacteria
• Eukaryotic cells: cells that contain a nucleus and other internal organelles • Ex: plant cells and animal cells
• Organelles: “tiny organs” inside the cell that help the cell carry out various processes and functions to keep it alive
![Page 35: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/35.jpg)
Organelles• Cell membrane
• Found in both plant and animal cells (and prokaryotic cells like bacteria)
• Surrounds the cell and provides protection • Controls what enters and exits the cell
![Page 36: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/36.jpg)
Organelles• Cell wall
• Found surrounding plant cells (eukaryotic) and bacteria cells (prokaryotic)
• Provides extra support and structure for the cell
![Page 37: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/37.jpg)
Organelles• Nucleus
• Found in both plant and animal cells
• Brain of the cell. Controls all cell functions. Where DNA is located
• Has a membrane around it, called the nuclear membrane
• Material (such as RNA) can enter and leave the nucleus
• Has an inner core known as the nucleolus where ribosomes are made
![Page 38: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/38.jpg)
Organelles• Mitochondria
• Found in both plant and animal cells• Known as the powerhouse of the cell because it makes energy for
the cell. Involved in cellular respiration.• Has two membranes surrounding it • Has folds inside, which are called cristae
![Page 39: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/39.jpg)
Organelles• Vesicles
• Found in both plant and animal cells• Small sacs or pouches that transport materials around the cell• Involved in exocytosis and endocytosis• Types
• Peroxisomes: found in both plant and animal cells. They break down fatty acid chains
• Lysosomes: found in only animal cells. Contain digestive enzymes that help clean up the cell
![Page 40: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/40.jpg)
Organelles• Ribosomes
• Found in both plant and animal cells• Made by the nucleolus• Attach themselves to the rough endoplasmic reticulum• Responsible for attaching to RNA and aiding in translation, which is
the process of making proteins
![Page 41: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/41.jpg)
Organelles• Rough endoplasmic reticulum
• Called rough ER for short• Found in both plant and animal cells• Responsible for transporting proteins.
• Smooth endoplasmic reticulum• Called the smooth ER for short• Found in both plant and animal cells• Responsible for breaking down fats and toxic substances
![Page 42: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/42.jpg)
Organelles
![Page 43: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/43.jpg)
Organelles• Golgi body (apparatus)
• Found in both plant and animal cells• Responsible for modifying proteins that are made by the rough ER
and ribosomes and then packaging them for distribution by vesicles
![Page 44: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/44.jpg)
Organelles• Chloroplasts
• Found only in plant cells• Responsible for preforming photosynthesis• Green in color because they contain the pigment chlorophyll
![Page 45: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/45.jpg)
Organelles• Vacuole
• Found in both plant and animal cells• Responsible for storing water and other important materials• Can expand or shrink depending on the needs of the cell
![Page 46: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/46.jpg)
PHOTOSYNTHESIS
Sunlight + 6CO2 + 6H2O C6H12O6 + 6O2
Reactants
Products
![Page 47: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/47.jpg)
Photosynthesis• The process of taking light energy and converting it into
chemical energy ( sugars)• 3 stages of photosynthesis
• Energy capture from sunlight• Light energy is converted into chemical energy and stored in a molecule of ATP or NADPH
• Chemical energy stored in ATP or NADPH is used to make organic compounds ( sugars)
![Page 48: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/48.jpg)
ATPA nucleotide with two extra energy-storing phosphate
groups
H20 + ATP ADP + P + energy Water + ATP adenosine diphosphate + phosphate + energy
Cell use the energy released by this reaction to power metabolism.
![Page 49: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/49.jpg)
![Page 50: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/50.jpg)
Plant parts• Stomata: openings in plant leaves and stems where gas
exchange occurs• Mesophyll: plant tissues• Chloroplasts: organelle that does photosynthesis
![Page 51: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/51.jpg)
Stroma: gel-like fluid inside chloroplastsGranum: stacks of thylakoidsThylakoid: site of photosynthesisLumen: empty space inside thylakoid
What is chlorophyll?• A pigment that absorbs red and blue light, and reflects
green light• This is why plants appear green• Chlorophyll is responsible for capturing the energy from
the sun
![Page 52: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/52.jpg)
Photo 1- light dependent reactions
• Stage 1• Energy capture from sunlight• Pigments are in disc-shaped structures called thylakoids• When light strikes a thylakoid, energy is transferred to electrons in
chlorophyll• This causes the electrons to have extra energy, they are said to be
“excited”
![Page 53: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/53.jpg)
Photo 1- light dependent reactions• Excited electrons jump to other molecules in the
thylakoid membrane• The electrons that left must be replaced• They are replaced by e- from water splitting apart• The oxygen left over is released into the atmosphere
as gas (we breathe it!)
![Page 54: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/54.jpg)
1. Light strikes thylakoid=excites electrons
E-E-E-
2. H20 splits a part. H+ is used to replace the electrons. O2 is released into the atmosphere
H2O
H+ electrons
O2 gas
Thylakoid
![Page 55: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/55.jpg)
![Page 56: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/56.jpg)
• Stage 2• Electron transport chains move the excited electrons along the
thylakoid membrane
![Page 57: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/57.jpg)
Photo 2- light dependent reactions
• Electron transport chains• Excited electrons pass through pumps in the thylakoid
membrane. They lose some of their energy• That energy is used to pump hydrogen (H+) ions into the
thylakoid• This creates a higher concentration of H+ inside than
outside, so they DIFFUSE back out of the thylakoid
![Page 58: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/58.jpg)
E-E-E-
1. Electrons leave the thylakoid. This creates energy
2. The energy is used to pump H+ ions into the thylakoid
H+H+H+H+
3. H+ ions diffuse out. This triggers the ATP reaction
Thylakoid
![Page 59: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/59.jpg)
Photo 2- light dependent reactions
• The H+ ions pass through carrier proteins in the membrane. (facilitated diffusion)
• The carrier proteins create a reaction in which a phosphate group is added to the chemical ADP, making ATP!
• This ATP is used to power the third stage of photosynthesis
![Page 60: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/60.jpg)
ADP + P ATP
Thylakoid
![Page 61: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/61.jpg)
Photo 2- light dependent reactions
• There is a second electron transport chain that provides energy to make another chemical called NADPH
• NADPH is an electron carrier that provides the high energy electrons needed to make carbon-hydrogen bonds in the third stage of photosynthesis
![Page 62: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/62.jpg)
![Page 63: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/63.jpg)
Overview of light dependent reactions• Pigment molecules in the thylakoids of chloroplasts
absorb light energy• Electrons in the pigments are excited by light and
move through electron transport chains in the thylakoid membranes
• These electrons are replaced by electrons from water molecules which are split
• Oxygen atoms and from water splitting combine to form oxygen gas
• Hydrogen ions accumulate inside thylakoids and then diffuse out which provides the energy to make ATP
![Page 64: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/64.jpg)
Stage 3-light independent reactions
• Does not require light• Carbon atoms from carbon dioxide (in the atmosphere)
are used to make organic compounds sugars!• Carbon dioxide fixation: transfer of carbon dioxide to
organic compounds
![Page 65: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/65.jpg)
Photo 2- Light independent reactions
Stage 3 The Calvin cycle (4 steps)1. in carbon dioxide fixation, each molecule of carbon dioxide (CO2) is added to a
five-carbon compound by an enzyme.2. The resulting six-carbon compound splits into 2 3-carbon compounds. Phosphate
groups from ATP and electrons from NADPH are added to these compounds, forming three-carbon sugars
3. One of the resulting three-carbon sugars is used to make organic compounds (starch and sucrose) which is stored by the organism
4. The other three-carbon sugars are used to regenerate the initial five-carbon compound, completing the cycle
![Page 66: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/66.jpg)
1.C-C-C-C-C
2.CO2 + C-C-C-C-C
3. C-C-C C-C-C
Stored as sugar for plant to use as food
Starts the carbon dioxide fixation process over again
![Page 67: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/67.jpg)
Factors that affect photosynthesis• The rate of photosynthesis increases as the intensity of
light increases, until all the pigments are being used. • At this “saturation point”, the Calvin cycle cannot move
any faster• Same thing happens after the saturation point of carbon
dioxide is reached
![Page 68: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/68.jpg)
OVERVIEW•Changing light energy into chemical energy
•Requires CO2 and H2O•Oxygen and glucose are the products
![Page 69: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/69.jpg)
CELLULAR RESPIRATIONTransferring energy from organic compounds
(sugars) to ATP
![Page 70: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/70.jpg)
Stages of Cellular Resp.• Stage 1: Called Glycolysis. Glucose is broken down into a
compound called pyruvate, producing a small amount of ATP and NADH
• Stage 2: Has different names depending on if oxygen is present and used in the process• Krebs Cycle: aerobic, oxygen is present• Fermentation: anaerobic, oxygen is absent
![Page 71: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/71.jpg)
Stage 1: glycolysis• Glucose is broken down in the CYTOPLASM (gel-like fluid
that surrounds all the organelles inside the cell)• Does not require oxygen, anaerobic• NADH plays the same role in cellular respiration that it did
in photosynthesis• It’s an electron carrier
![Page 72: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/72.jpg)
Steps of glycolysis• 1) phosphate groups from 2 ATP molecules are
transferred to a glucose molecule• 2) the resulting 6-carbon compound is broken down into 2,
3-carbon compounds, each with a phosphate group• 3) two NADH molecules are produced• 4) each 3-carbon compound is converted to a 3-carbon
pyruvate, producing 4 ATP molecules
![Page 73: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/73.jpg)
![Page 74: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/74.jpg)
Stage 1: glycolysis• Glycolysis uses 2 ATP, but generates 4• Glycolysis is followed by another set of reactions that use
the energy temporarily stored in NADH to make more ATP
![Page 75: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/75.jpg)
Glycolysis reactants & products
![Page 76: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/76.jpg)
Stage 2: Aerobic• Aerobic means that oxygen is required to run the process• We call aerobic respiration of stage 2, the Kreb’s Cycle
![Page 77: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/77.jpg)
Kreb’s Cycle• 1) Acetyl-CoA combines with a 4-carbon compound,
forming a 6-carbon compound and releasing coenzyme A
![Page 78: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/78.jpg)
Kreb’s Cycle• 2) carbon dioxide, CO2, is released from the 6-carbon
compound, forming a 5-carbon compound. Electrons are transferred to NAD+, making a molecule of NADH
• 3) carbon dioxide is released from the 5-carbon compound, resulting in a 4-carbon compound. A molecule of ATP is made, and a molecule of NADH is also produced
![Page 79: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/79.jpg)
Kreb’s Cycle• 4) the existing 4-carbon compound is converted to a new
4-carbon compound. Electrons are transferred to an electron acceptor called FAD, making a molecule of FADH2 (electron carrier)
• 5) The new 4-carbon compound is then converted to the 4-carbon compound that began the cycle. Another molecule of NADH is produced
![Page 80: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/80.jpg)
Chemicals and their jobs• Electron Acceptors: NAD+ and FAD• Electron Carriers: NADH and FADH2
• Chemical form of energy: ATP• Equations
• NAD+ + electron NADH• FAD + electron FADH2
• ADP + P ATP
![Page 81: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/81.jpg)
Electron Transport Chains• 1) Hydrogen ions (H+) are pumped out of the inner part of
the mitochondria• 2) Electrons and hydrogen ions combine with oxygen,
forming water• 3) ATP is produced as hydrogen ions diffuse into the inner
part of the mitochondria through a channel protein (facilitated diffusion)
![Page 82: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/82.jpg)
![Page 83: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/83.jpg)
Stage 2: anaerboic• Anaerobic means that oxygen is not required to run the
process• Anaerobic respiration occurs when there is not enough
oxygen to run the Kreb’s Cycle• This process is called Fermentation. There are two types
• Lactic Acid Fermentation• Alcoholic Fermentation
• In this process, NAD+ is recycled and allows glycolysis to continue. A small amount of ATP is produced
![Page 84: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/84.jpg)
Lactic Acid Fermentation
• A 3-carbon pyruvate is converted to a 3-carbon lactate through lactic acid fermentation
• Lactate is the ion of an organic acid called lactic acid
• Fermentation enables glycolysis to continue producing ATP as long as the glucose supply lasts.• Lactic acid builds up in your muscles and
is removed by your blood. Lactate can build up in your muscle cells if it is not removed quickly enough, sometimes causing sore muscles!
• Bacteria and other animals that are anaerobic use LAF
![Page 85: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/85.jpg)
Alcoholic Fermentation• The 3-carbon compound
pyruvate is broken down to ethanol, a 2-carbon compound.
• Carbon dioxide is released during the process
• Alcoholic fermentation by yeast, a fungus, has been used in the preparation of many foods and beverages• Wine and beer making• Carbon dioxide released by yeast
causes dough to rise Carbon dioxide released by yeast causes carbonation in beer
![Page 86: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/86.jpg)
ATP Production• Glycolysis: 2 ATP molecules gained• Aerobic respiration: 2 ATP molecules gained• Electron transport chain: 34 ATP molecules gained
•TOTAL= 38• Fermentation produces a small amount of ATP
![Page 87: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/87.jpg)
DNA• Name: Deoxyribonucleic Acid• Structure:
• Double helix “twisted ladder”• Phosphate and sugar “backbone”• Nitrogen bases that form complimentary pairs
• Adenosine (A) Thymine (T)• Guanine (G) Cytosine (C)• Hydrogen bonds join the bases together• A and G are purines• T and C are pyrimidines
![Page 89: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/89.jpg)
DNA• Function:
• Carries a genetic code (order of the bases like agctatgca…)• This genetic code is the recipe for how to make specific proteins• Those proteins assemble into parts of the cell and body to make up
unique traits such as hair color, eye color and skin color
![Page 90: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/90.jpg)
DNA• Genes
• Genetic code: the order of the bases. • Different order=different people and even different species!
• Gene: a segment of DNA that codes for a protein• Chromosome: a long chain of DNA all coiled up
• Humans have 46 chromosomes, each with 1,000’s of genes on them• Other species have different amount of chromosomes
![Page 91: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/91.jpg)
DNA• Making more DNA= DNA replication• DNA needs to make copies of itself so that every new cell
that is made, has a copy of DNA• You make new cells all the time (new skin cells daily!)
• How does DNA replicate?• 1. An enzyme, called DNA helicase, binds to DNA and unzips it• 2. DNA helicase unzips the DNA molecule by breaking hydrogen
bonds between the bases• 3. Another enzyme, DNA polymerase, binds to DNA and adds NEW
bases to each side of the DNA “ladder”
![Page 92: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/92.jpg)
ATGGCTAATCCGTTACCGATTAGGCA
TAGGCCT
ATCCGGA
DNA helicase
![Page 93: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/93.jpg)
ATGGCTAATCCGTTAGGCCT TACCGATTAGGCAATCCGGA TACCGATTAGGCAATCCGGA ATGGCTAATCCGTTAGGCCTDNA polymerase
![Page 94: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/94.jpg)
DNA• Sometimes, DNA polymerase makes mistakes
• A G by accident!• Proof-reader enzymes are responsible for checking the newly
added strand for such mistakes
![Page 95: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/95.jpg)
DNA• One of DNA’s roles is the recipe for making protein• How does DNA make proteins?
• First off, DNA cannot leave the nucleus. Proteins can only be made in the cell’s cytoplasm and if DNA enters the cytoplasm, it will start to break down.
• So, to solve this problem, the cell uses another molecule that is similar to DNA. This molecule carries DNA’s recipe or message into the cytoplasm so that proteins can be made
• The molecule is called RNA
![Page 96: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/96.jpg)
RNA• Name: Ribonucleic Acid• Structure:
• A single helix (one sided “ladder”)• A phosphate and sugar “backbone”• Nitrogen bases that form complimentary pairs
• Adenosine Uracil (NOT THYMINE)• Guanine Cytosine
![Page 97: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/97.jpg)
![Page 98: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/98.jpg)
Transcription• Transcription occurs when RNA makes a copy of DNA
• Why does this happen? Because DNA cannot leave the nucleus, and protein are made OUTSIDE of the nucleus, in the cell’s cytoplasm
• Steps• 1. DNA is unzipped by the enzyme DNA helicase• 2. The enzyme RNA polymerase, begins to add complimentary
RNA bases to ONE STRAND of DNA• 3. The newly made RNA strand detaches, and leave the nucleus
• This strand of RNA is specifically called messenger RNA, or mRNA
![Page 99: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/99.jpg)
Transcription
![Page 100: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/100.jpg)
Translation/protein synthesis• What happens to RNA after it leaves the nucleus?
• It travels into the cell’s cytoplasm and the process of translation begins.
• Translation is also called protein synthesis (the making of proteins)• What are the steps of translation?
• 1. mRNA hooks up to a ribosome• 2. A different type of RNA, transfer RNA (tRNA) brings
complimentary bases and attaches to the mRNA• 3. tRNA is also carrying amino acids. Amino acids are the building
blocks for proteins• 4. When the bases of mRNA and tRNA are joined, the amino acid
carried by tRNA pops off• 5. Amino acids start to form chains, called polypeptides (a.k.a
proteins)
![Page 101: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/101.jpg)
Translation/protein synthesis• When tRNA attaches to mRNA, it does so through
anticodons• mRNA has codons, which are a group of 3 bases
• AUG• tRNA has anticodons, which are a group of 3 bases that are
complimentary to the codons on mRNA• UAC
• A codon is what “codes” for one amino acid
• mRNA codons
• Amino acids
![Page 102: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/102.jpg)
Translation/protein synthesis• Amino acids
• There are 20 amino acids• Some codons “code” for the same amino acid• Some codons “code” for the cell to stop or start producing amino
acids
![Page 103: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/103.jpg)
Cytoplasm
Nucleus
Ribosome
Amino acid
tRNA
Anticodon
mRNA
Chain of amino acids=polypeptide
![Page 104: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/104.jpg)
Mutations• There can be mistakes that occur along the way
• During DNA replication• During Transcription• During Translation
• Types of mutations• Point mutations-one base of DNA/RNA is altered
• Substitution, insertion, deletion• Can cause a frame-shift mutation
• Chromosome mutation-a segment or whole chromosome is altered
![Page 105: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/105.jpg)
Mutations
• Substitution: when one base is substituted for another
• Deletion: when one, or more, base is deleted
• Insertion: when one, or more, base is added
• Both insertions and deletions can cause frame-shift mutations• Frame-shift mutations cause codons to be read differently. These
changes can cause different amino acids to be coded for, which will lead to incorrect protein assembly
![Page 106: Keystone review 1](https://reader036.fdocuments.in/reader036/viewer/2022062410/56815f4d550346895dce2aad/html5/thumbnails/106.jpg)
Mutations• Chromosome mutations
• Deletion- part of a chromosome is deleted
• Duplication- part of a chromosome is doubled
• Inversion- part of a chromosome flips around and reattaches
• Translocation- non-homologous pairs exchange chromosome segments