Keynote 3: Translational Science and the Microbiome...Plan of talk • Disease association processes...
Transcript of Keynote 3: Translational Science and the Microbiome...Plan of talk • Disease association processes...
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Translational Science and the Microbiome
Jonathan Braun, M.D., Ph.D. David Geffen School of Medicine University of California, Los Angeles
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Eli
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2007: Exceptional Scale and Diversity of Bacterial Phyla in Healthy Humans
Relman, Nature. 2007 449:811
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Benedetto Bordon, 1528 http://maps.bpl.org/
Microbiome, 2007
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2012: The microbiome “aerial view” is complete
HMP Consortium, Nature 2012
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Like host genetic variants, our microbiomes are unique and (somewhat) stable
7
40 genes in the “same” microbe, Prevotella copri
Present
Absent
Microbiome variants are genetically distinct and persistent up to a period
of at least months.
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Diet affects composition of the microbiome only in the long-term
8
CAFE n=10 subj,
10 days Claesson 2012, Nature
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Effects of birth mode, lifetime, and geography on the microbiome
9
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Plan of talk • Disease association processes of the microbiome
• Hit and run (the neonatal window) • Good and bad guys (C. difficile, atherosclerosis) • Good and bad ecosystems (obesity, behavior, IBD, systemic lymphoma)
• Functional ecology • The phylogeny paradox • Co-occurrence networks of bacteria, proteins, and metabolites • Functional analysis of ecosystems
• Fixing radios, and the way forward • Ecosystems repair • Targeting functional features of the microbiome • Systems versus pathways
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Hit and Run: neonatal microbiome has life-long effect on elements of immune function and obesity
Cho I, Nature 2012 Trassande J, I J Obesity 2013
Neonatal antibiotics and body fat Olszak T, Science 2012
iNKT cells and risk of colitis, asthma
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Tang WHW, N Engl J Med 2013
Basal Antibiotcs After antibiotcs
Good and Bad Bugs
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Tang WHW, N Engl J Med 2013
Good and Bad Bugs
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Ecosystem disruption and C. difficile colitis
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Lemon KP, Sci Transl Med 2012
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Cox LM, Cell Metabolism 2013
Alternate ecosystems Microbiome, direct and indirect, in obesity
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Cox LM, FASEB J 2013
Prebiotic modification of microbiome “Neutralization” of high fat-induced obesity with complex carbohydrates
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Cryan JF Nat Rev Neuroscience 2012
Microbiome and behavior
Tillisch Gastroenterology 2013
Short-term yogurt ingestion and response to emotional stimuli
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Ataxia Telangiectasia (AT)
Clinical manifestation: Autosomal recessive disease (1 in 40.000-100.000 people affected) Early-onset progressive cerebellar ataxia High incidence of tumors (1 in10 develop lymphoma or leukemia) Growth retardation Immunodeficiency
Biological markers: Chromosomal instability Hypersensitivity to radiation Imbalance in antioxidant levels and antioxidative enzymes
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Lymphoma latency in ATM-/- mice is modified by microbial composition
Rustic lifestyle microbiota (RF) Urban lifestyle microbiota (CF)
Wei B, J Immunol 2010 Yamamoto ML. Cancer Res 2013
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Systemic lymphocyte DNA damage and oxidative stress is modified by microbial composition
Yamamoto ML. Cancer Res. 2013
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Identification of microbiota selectively augmented or depleted in lymphoma resistant RM microbiota
UniFrac (PCA) Candidate organisms (2 of 8)
Candidate prioritization • Contribution to PC1 and 2 • False-detection significance of association • Consistent presence and correlation in individual mice • Relevant biologic features • Culturability
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Lactobacillus johnsonii reduces genotoxicity and inflammatory state of lymphoma-prone (CM) ATM-/- mice
Hemopoietic DNA damage
Systemic inflammatory cytokine levels
Hepatic innate lymphocyte levels
Yamamoto ML. Cancer Res. 2013
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Microbial composition and lymphoma
• Ataxia Telangiectasia, a DNA repair disease, results in highly penetrant lymphoma
• Mucosal inflammation induces systemic DNA damage
• Lymphoma and survival in ATM-/- mice is dependent on intestinal microbiota
• Microbiota identified that fulfill Koch’s postulate for correcting mucosa-associated inflammation and systemic genotoxicity
• Microbial composition is a modifiable factor in lymphoma
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Mouse with genetic risk No gut bacteria
Mouse with genetic risk Enterobacter faecalis
Mouse with genetic risk Bacteroides fragilis
Microbial composition and IBD
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IBD risk factors and microbiome composition
Morgan, Genome Biology 2012 Morgan, Genome Biology 2012
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Morgan X, Genome Biol 2012
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Correlation vs. Causation
• Better cohort study design • Integration of habitat and
remote biologic metadata
• Mechanistic definition and validation of microbial function
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Justus Kanckerts 1600 http://maps.bpl.org/
Microbiome, 2013 (Western Hemisphere, 1600)
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Plan of talk • Disease association processes of the microbiome
• Hit and run (the neonatal window) • Good and bad guys (C. difficile, atherosclerosis) • Good and bad ecosystems (obesity, behavior, IBD, systemic lymphoma)
• Functional ecology • The phylogeny paradox • Co-occurrence networks of bacteria, proteins, and metabolites • Functional analysis of ecosystems
• Fixing radios, and the way forward • Ecosystems repair • Targeting functional features of the microbiome • Systems versus pathways
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16S view of individual microbial composition
Tong M, in press
Bacteroides Parabacteroides Escherichia Prevotella Faecalibacterium
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Functional diversity within a single genus (Solanales)
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Functional diversity within a single species
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A large portion of genes (~50%) correlate well at the DNA and RNA levels
*Genes are KEGG Orthogroups, KOs
0
0.25
0.5
0.75
1 Dorea
Bifidobacterium
Parabacteroides
Roseburia
Coprococcus
Ruminococcus
Faecalibacterium
Alistipes
Bacteroides
Eubacterium 0
0.0025
0.005
0.0075
0.01
0
0.1
0.2
0.3
Genera Genes (3rd lev. EC) Transcripts (3rd lev. EC) How consistent are the top ten...
• Microbial membership varies. • Early colonization? Genetics?
• Over time, the community “solves” for a habitat-specific metagenome.
• It then differentially regulates that metagenome. • These two types of regulation differ at least in time scale.
Relating the gut metagenome and metatranscriptome
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Analytical Pipeline
1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00
%
0
100
3.85605
1.54154
0.841200.39
102
1.40188
3.63237
3.44621
2.58236
1.96176
2.97255
3.98429
4.85133
9.4982
5.17288 7.30
5246.52496
9.056668.69
3817.97637
Metabolites, proteins
Colonic samples 100s of patients and controls
PICRUST HUMAnN
OTUs
KEGG functional profiling Metabolite comparisons with bacteria and human proteins
Imputed metagenome
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Microbiota are wired to the local metabolic milieu
Bacteria x Human Proteins Bacteria x Metabolites Human Proteins x Metabolites
McHardy, Microbiome2013
1 2 3
16S OTUs M
etab
olite
s Positive Correlation
Negative Correlation
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Functional specialization of microbiota in Crohn’s disease and ulcerative colitis
CD UC HC
Tong M, in press
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Morgan X, Genome Biol 2012
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Mucosal inflammation: a genetic and microbial process in intestinal neoplasia and systemic disease
Immune Regulation
Epithelial Barrier
Bacterial Control
Cellular Stress Nature (2011) 474:307
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Do we genetically “garden” or microbiome? Human QTL analysis for 115 highest abundance bacterial taxa
Tong M, in preparation
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Johansson MV, PNAS 2010
Intestinal Wall
Lumen (food)
• COSMC • Core 1 O-glycanase • Fut2
Mucin O-glycan genetics and IBD
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WT Fut2-/-
SE se
PICRUST HUMAnN
PICRUST HUMAnN
Microbial composition
in members of each genetic group
Differential microbial
genes
FUT2 risk polymorphism (null) in healthy individuals
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29 24 Enriched in Secretor Individuals
Enriched in Non-Secretor Individuals
12
Human (SE vs. se)
Mouse (WT vs. Fut2-/-)
25 22 6
KEGG pathway genes concordant in human and mouse microbial communities after FUT2 gardening
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FUT2 Polymorphism Pervasively Affected the Metabolic Functions Encoded by Gut Microbiome
Cysteine and methionine metabolism
Lysine biosynthesis
C5-Branched dibasic acid metabolism
Pantothenate and CoA biosynthesis
Porphyrin and chlorophyll metabolism
Terpenoid backbone biosynthesis
RNA transport
Penicillin and cephalosporin biosynthesis
Phenylalanine metabolism
Glutathione metabolism
Lipopolysaccharide biosynthesis
Arachidonic acid metabolism
Biotin metabolism
Enriched in SeSe individuals
Enriched in Sese and sese individuals
SeSe/FUT2+/+ Sese/FUT2+/- Sese/FUT2-/-
0.000 0.010 0.020 0.0300.000 0.010 0.020 0.030
Human dataset Mouse dataset
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Plan of talk • Disease association processes of the microbiome
• Hit and run (the neonatal window) • Good and bad guys (C. difficile, atherosclerosis) • Good and bad ecosystems (obesity, behavior, IBD, systemic lymphoma)
• Functional ecology • The phylogeny paradox • Co-occurrence networks of bacteria, proteins, and metabolites • Functional analysis of ecosystems
• Fixing radios, and the way forward • Ecosystems repair • Targeting functional features of the microbiome • Systems versus pathways
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Repairing the ecosystem
• Experimental observations and ecological principles suggest that disease states are not explained by individual organisms
• The disease-associated microbiome is powerfully described by functional features
• This makes sense mechanistically – Microbial products drive disease (or protective) host states – Inter-microbial interactions determine composition and function
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Repairing the ecosystem
• Selectively deplete pathobionts?
• Add back deficient benefibionts?
• Intervention by targeting functions – Mass action: substrate or end-products – Pathway inhibitors – Alien functionalbionts
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Alternate ecosystem states A multi-stable, multi-factorial system
Mic
robi
ota
Ecol
ogy
“HEALTHY” “DISEASE”
“PERTURBATION”
Host genetics and environmental/physiological factors shape the contours of the landscape and determine an individual’s disease susceptibility or tolerance
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Challenges
• We need robust knowledge of ecosystem networks – What are the set of stable ecosystem states? – How do we navigate between them?
• What are the microbiome problems in each disease? – A hit and run event (obesity, NKT cell immunity) – A broken or altered component (C. difficile, atherosclerosis) – The wrong ecosystem state (obesity, IBD)
• Will hub or causal targets be more robust for intervention?
• We need the right analytics to define and testt systems – Easy, robust sampling and preanalytic platforms – Quantitative functional measurements (transcripts, proteins, metabolites) – Component testing experimental design
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Harnessing the microbiome by microbial product targeting (2016)
• 250 million people • Maps have vast amounts and types of data • Map data accessible by everyone • Useful in amazing ways
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Braun Lab • Jonathan Braun • Michelle Li • Ian McHardy • Maomeng Tong • Bo Wei Mouse O-glycan biology • Lijun Xia (OMRF) • Justin Sonnenberg (Stanford) CCFA Microbiome Consortium • Dermot McGovern • Ramnik Xavier • Curtis Huttenhower • Balfour Sartor • Thad Stappenback • Skip Virgin
MLI consortium • Dermot McGovern • James Borneman • Tom Graeber • Al Fornace • Justin Sonnenburg
Acknowledgements
DNA damage and AT • Robert Schiestl (UCLA)