KESRRP001 RT- PCR report2009. 07. 07

1. Sample check

C sample

C -> control 1.5ug loading

# Sample 260/280 260/230 conc.(ng/ul) Total(ul) Total(ug) loading양

1 Kenaf 1.87 1.99 2293.5 45 103.2 2.3ug

2. Primer sequence

2009. 07. 07

KESRRP001 RT- PCR report

No. name primer sequence primer 수 PCR product size(bp)

1LuCAD-F catggaaaagggaagaacca 20

1052LuCAD-R acggctgctgaagatcactt 20

2MsCAD-F gcattgaagctgcagaaaga 20

1067MsCAD-R tcctttgacatccacaacga 20

3HcC4H-F aagtcggcgatgacttgaac 20

1317HcC4H-R gtccacctttctccgtcgta 20

4GaC4H-F ttcctagagaaagttctcatctctctt 27

1506GaC4H-R tcaaaacacccttggctttg 20

5StC4H-F tctcttactggagaagaccttaatagg 27

1503StC4H-R gatcttggtttcatcacaatgg 22

6PtC4H-F tcctggagaagaccctcttg 20

1504PtC4H-R taaaaggaccttggctttgc 20

7HcCCR-F gtggagccagccgtgaat 18

475HcCCR-R cacggtggagaacactctcg 20

8EgCCR-F1 atgcccgtcgacgccctc 18

1009EgCCR-R atccctgaatacgcaccgaat 21

8ㅡ 1EgCCR-F2 gttcatcgcctcctggatt 19

950EgCCR-R atccctgaatacgcaccgaat 21

9LeCCR-F gtcgggtcgtctgtgttacc 20

981LeCCR-R aagtctgaattcgaataatttcgtc 25

10StCCR-F catcagaatccggcaaagtt 20

991StCCR-R gagtgaattttaataggttcatcattt 27

Temp. Time CyclesFinal Reaction

vol.

95 ℃ 10min 1

50ul

94 ℃ 30sec

4042~60 ℃

30sec

72 ℃1min

30sec

72℃ 10 min 1

4℃Storag

e 1

3. PCR Condition

2009. 07. 07

KESRRP001 RT- PCR report

4. The Result of PCR

2009. 07. 07

KESRRP001 RT- PCR report

1kb 1 2 3 4 5 6 7 8 8-1 9 10 3 1kb 4 1kb 7 1kb

No. name PCR product size(bp)

1LuCAD-F

1052LuCAD-R

2MsCAD-F

1067MsCAD-R

3HcC4H-F

1317HcC4H-R

4GaC4H-F

1506GaC4H-F-R

5StC4H-F

1503StC4H-R

No. name PCR product size(bp)

6PtC4H-F

1504PtC4H-R

7HcCCR-F

475HcCCR-R

8EgCCR-F1

1009EgCCR-R

8ㅡ 1EgCCR-F2

950EgCCR-R

9LeCCR-F

981LeCCR-R

10StCCR-F

991StCCR-R

V V

V