Jurriaan Mes Wageningen - Food & Biobased Research Food ... · PTR-QiTOF analysis and applications...
Transcript of Jurriaan Mes Wageningen - Food & Biobased Research Food ... · PTR-QiTOF analysis and applications...
PTR-QiTOF analysis and applications
Jurriaan Mes
Wageningen - Food & Biobased Research
Food & Health Research
Liver disease
Diagnose chronic liver disease (CLD) patients, compensated cirrhosis (CIR) from those without cirrhosis
Scientific Reports 6, Article number: 19903 (2016)
Nr. Chemical identity Change
1 3-methylbutanal (−)
2 Propanoic acid (+)
3 Octane (+)
4 Terpene (C10H16) (+)
5 Terpenoid: α-pinene (+)
6 3-carene (+)
7 Unknown (+)
8 Branched C16H34 (+)
9 1-hexadecanol (−)
10 Branched C16H34 (−)
11 Dimethyl disulfide (+)
IBD children
Aliment Pharmacol Ther. 2014 Sep; 40(5): 498–507.
What we did
Developed protocol● Effect of pre-digested food/ beverages● How long and how often to breath in tube● Correct background VOC
SenTo Panel breath marker development
T2D Asthma Gut complains
Can we correlate VOC from exhaled breath with health status● Which biomarkers can be analysed direct in breath● How strong /predictive are biomarkers● How specific are biomarker for health status
● Single marker or based on model ● Combine with other (blood based) biomarker
Breath analysis update
Medical Ethical Approval received● Had to show the accuracy and sensitivity of the PTR-QiTOF ourselves
Recruitment and analyse Target 25 persons in each group Analyse breath 1x per month, 4x months Questionnaire on health at same time (variable over
time) Now evaluating the data Next
● Continue with same population
● Include also another health issue
Problems and opportunities
Problems Data analysis from raw to
processed
● Need to be more automated
Need support from processed to biomarker
● Statistics, biometris
Opportunities Quick diagnose of diseases,
inflammation, cancers etcfor hospitals
Easy assessments of health status and effect of an (food) intervention/ treatment
Promising direction for sensors when validated biomarkers and models for translation have been developed