Jordan Garth portfolio

JORDAN GARTH UNDERGRADUATE PORTFOLIO

A summary of a few important projects done while in undergraduate at Georgia Institute of Technology

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Table of Contents •  Lab for Neuroengineering à Slide 3 • BMED 2300 Problems in Engineering II (Sophomore

Design) à Slide 13 • GTRI ELSYS Co-op II Summer 2014 à Slide 35 • GTRI EOSL Co-op III Spring 2015 à Slide 54

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Lab for Neuroengineering •  I worked in the Lab for Neuroengineering from January

2012-December 2012 •  The following slides are a summary of my work from that

time

Lab for Neuroengineering

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U87MG-PE-GFP CANCER CELL LINE IN VITRO

Jordan Garth Undergraduate Research Fall 2012

Georgia Institute of Technology Bellamkonda Lab for Neuroengineering

Mentor: Martha Betancur November 28, 2012


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Research This Semester • DARPA Project

•  Researched the failure mechanisms of neuronal electrodes •  Sectioning •  Staining •  Imaging •  Image Analysis

• Eureka Project •  ‘Exvading’ inoperable brain tumors in the cerebellum •  Goal à to engineer a path of least resistance in using PCL

nanofiber conduits for treatment


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Using the U87MG-pe-GFP Cell Line

• Why do we use U87MG-pe-GFP cancer cell line? • Able to inoculate in vivo and measure the tumor that develops

• Common human glioblastoma cell line Tissue from an inoculated animal


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Testing Ki67 and PCNA on U87 Cells • We cultured the U87 cells into the slides to test Ki-67 and

PCNA • Ki-67 is a protein used to measure proliferation of the cells

•  Present in all cells life cycles except for G0 • PCNA is a protein present during DNA synthesis • Goal à calculate proliferation rate in the cells


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Cell Culture Overview

•  Incubate PS – 2 slides • Make complete media • Put frozen cells into the water broth • Cells adhere to the bottom • Washed with PBS, add trypsin to pick up the cells • Place in new media for an allotted time


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Imaging

Ki67

GFP

DAPI

Merged image of Ki67 10X (48 hr) Merged image of PCNA 10X (48 hr)


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Imaging Continued

Ki67 40X (48 hr) PCNA 40X (48 hr)


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Image Analysis

0

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Cell Count

Dapi cell Count from Ki67

GFP cell Count from Ki67

Dapi cell Count from PCNA

GFP cell Count from PCNA

Average Cell Count

0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

Index (%cells not in G0)

Ki67 index From Dapi

Ki67 index from GFP

PCNA index from Dapi

PCNA index from GFP

Percentage of Cells Proliferating

Index = (Cells with proteins)/(Total cells detected with DAPI or GFP)

N=9 images per staining


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Future Studies

•  It is now clear to use Ki-67 to detect proliferation in U87MG-pe-GFP

•  Next step à use this stain in vivo sections •  Get the proliferation rate using Ki-67 in U87MG-pe-

GFP in the empty versus the aligned nanofilm conduits

•  I suspect that the proliferation rate of the cells is hypothesized to be less in vivo than in vitro •  In vitro the cells have much more media to feed off of,

whereas in vivo, the tumor cells are more starved


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BMED 2300: Problems in Biomedical Engineering II (Design Course) •  The objective of the course was to choose a medical

device to redesign to better fit a user’s needs •  This is a sophomore design course taken in a group,

which I took in Spring 2013

BMED 2300

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Introduction •  Football helmets prevent

the probability of sustaining brain injury •  Designed to absorb forces

upon impact and slow down the reaction

•  Strategically placed padding • Concussions are a

commonality amongst football players

• Significant metabolic derangement that occurs immediately after a concussion

BMED 2300

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• High school football player with special conditions •  I.e. mood disorders,

previous concussions, physical abnormalities

• Require specific needs • Easier access •  Increased protection

Users

http://photos.pennlive.com/patriot-news/2012/08/2012_high_school_football_play_18.html

http://upload.wikimedia.org/wikipedia/commons/6/6e/MRI_of_human_brain_with_type-1_Arnold-Chiari_malformation_and_herniated_cerebellum.jpg

Individual with Arnold Chiari Malformation

BMED 2300

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Problem Statement • With more than 7.6 million high school students play

football, with average player sustains an estimated 950 impacts to the head during a season

• Both athletic trainers and athletes feel significant pressure to return athletes to practice and play as soon as possible after injury

•  Statement: Redesign the football helmet with increased protection and ease of access

BMED 2300

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Objectives • Absorb the shock from hits, and prevent the most amount

of damage possible •  Lower probability of concussions and brain injury • Meet NOCSAE regulations • Provide cost efficiency, durability, be aesthetically pleasing

and lightweight

BMED 2300

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Ranked Constraints

BMED 2300

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Engineering Analysis

BMED 2300

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Engineering Analysis

BMED 2300

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Iterations

Kevlar Layer

• Pros: Shock absorption, energy dissipation

• Cons: Cost inefficient, friction if it is too thick

Thicker Pads, Larger Helmet

• Pros: More protection, less impact

• Cons: Additional weight, decreased peripherals

BMED 2300

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Iterations

Pull Apart Helmet

• Pros: Ease of Access • Cons: Cost, Could open

up during game play

Light Sensor

• Pros: Detects size, perfect fit

• Cons:Extra Cost

BMED 2300

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Iterations

Hinged Sides

• Pros: Easy access, comfortable fit

• Cons: Added weight, could fall off with overuse

Hinged Facemask

• Pros: Easy Access • Cons: Extra Cost,

Facemask could come off

BMED 2300

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Iterations

Rubber Stoppers with Internal Cap

• Pros: Added protection and firm placement

• Cons: Cost, less space for head inside of helmet

Rubber Stoppers

• Pros: Firm placement • Cons: Cost

BMED 2300

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Iterations

Spiderweb padding system

• Pros: Distributes force evenly, provides better fit

• Cons: More material means more expensive

BMED 2300

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P2 Prototype

Network of Pads

Hinged Sides

Hinged Facemask

Kevlar layer

BMED 2300

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Experienced User Interview •  "As a former football player who experienced multiple concussions, I think the network of pads under a layer of Kevlar will significantly help reduce impact force and allow players whose conditions could be worsened by head injury to play like normal.“

-Taylor Nicholson

•  "Being a trainer, I know that accessibility to an injured player is important. Though the hinged sides may make it too easy for the helmet to come off during play, the hinged face mask provides just the right amount of accesibility for a trainer to attend to player who needs help."

-Dana Domangue BMED 2300

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P3 Final Design • Hinged Facemask • Spiderweb Padding System •  Thin Layer of Kevlar • Rubber Stoppers

BMED 2300

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Solidworks

BMED 2300

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SolidWorks: Rubber Stopper

BMED 2300

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SolidWorks: Frontal Padding

BMED 2300

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SolidWorks: Padding

BMED 2300

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SolidWorks: Shell

BMED 2300

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SolidWorks: Kevlar strip

BMED 2300

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GTRI ELSYS Co-op II Summer 2014

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Projects • Balance study in conjunction with Georgia Tech using

ballerinas and novices •  ISSS working on patient status monitor

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Project 1: Ballerinas •  In this study:

•  The subject wears markers that reflect from LED infrared lights to show position in space

•  GTRI provides accelerometer to find orientation of the dancer and acceleration in the body frame

• Current contribution: •  Installation of sensors, data collection, troubleshooting, and general

support

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Project 1: Ballerinas cont. •  Future contribution: data analysis

•  Goal from the data is to get the overall body acceleration from the body frame angular rate and body frame angular acceleration

Using the laws of motion in a rotating reference frame

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Project 2: ISSS PSM Beginning

•  Three main watch comparisons

Basis Mio Alpha Adidas MiCoach

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Project 2: ISSS PSM Feedback •  Wearable devices on the wrist are popular (solar watches) •  Charging strips are limited on deployment (lasting up to days

at a time), and therefore power could make some solutions impractical

•  Adidas was through to be uncomfortable due to its wide form factor

•  Overall, it was decided that preferences would lean towards a BASIS functionality in a Mio profile with a Mio band.

•  Some concern exists that larger watch faces will snag of cuff buttons and be difficult to access

•  Gloved operation was not an issue, although the BASIS was preferred because one could be less precise.

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Project 2: ISSS PSM Testing •  Basis versus Mio Alpha compared on pulse oximeter •  Resting HR Procedure:

•  Put both on approx at same time, Wait 1 min, Record •  Elevated HR procedure:

•  20 jumping jacks, Wait 15 seconds, Record

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Project 2: ISSS PSM Testing • Same procedure compared to ECG

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Project 2: ISSS PSM conclusion 1 • Mio Alpha based on its superior accuracy is the best

watch •  Now onto additional testing and redesigns

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Final testing: Mio Alpha •  Test that simulates soldier conditions:

•  Resting HR for 30 minutes •  50% HR (for 20-30 year olds, this is between 100-130 bpm) •  80% HR (for 20-30 year olds, this is between 150-180)

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Resting Heart Rate

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100

10:3

6:51

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:37:

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10:3

9:11

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:40:

01

10:4

1:21

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:42:

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10:4

4:01

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:45:

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10:4

6:31

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:47:

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10:4

9:11

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:50:

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10:5

1:31

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:52:

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10:5

4:41

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:56:

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10:5

7:51

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:59:

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11:0

0:01

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:01:

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11:0

2:51

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:04:

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11:0

6:01

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:07:

01

Mio HR

GE HR

Kyle Land Resting HR

Average Error: 4.992092

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100

14:4

4:43

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:46:

03

14:4

7:03

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:48:

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14:4

9:03

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:49:

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14:5

1:03

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:52:

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14:5

3:13

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14:5

5:13

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14:5

7:33

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15:0

0:04

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:01:

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15:0

2:13

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:03:

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15:0

4:04

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15:0

6:33

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15:0

8:33

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:09:

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15:1

0:33

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:12:

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15:1

3:14

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:14:

13

15:1

5:13

John McClure Resting HR

mio ge

Average Error 4.621705

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50% Heart Rate

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120

140

11:1

2:59

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:13:

39

11:1

3:59

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:14:

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11:1

4:49

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:15:

09

11:1

5:29

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:15:

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11:1

6:09

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:16:

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11:1

7:19

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:17:

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11:1

7:59

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:18:

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11:1

8:39

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:19:

09

11:1

9:29

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:19:

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11:2

0:19

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:20:

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11:2

0:59

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:21:

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11:2

1:49

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:22:

09

11:2

2:29

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:22:

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Kyle Land Medium HR

mio ge

Average percent error: 1.418672

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40

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120

140

160

John McClure HR

Mio GE

2.139142 Average percent error:

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80% Heart Rate

0

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100

120

140

160

180

11:2

6:04

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:26:

24

11:2

6:34

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:26:

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11:2

7:34

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:27:

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11:2

7:54

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:28:

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11:2

9:04

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:29:

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11:2

9:34

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:29:

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11:2

9:53

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:30:

04

11:3

0:14

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:30:

24

11:3

0:44

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:30:

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11:3

1:03

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:31:

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Kyle Land

MIO GE


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11:0

0:47

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:00:

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11:0

1:06

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:01:

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11:0

1:27

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:01:

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11:0

1:57

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:02:

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11:0

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:02:

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11:0

3:07

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:03:

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11:0

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:03:

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11:0

3:47

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:03:

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11:0

4:26

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11:0

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11:0

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11:0

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11:0

5:46

John McClure


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Redesign 1: Basic

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Redesign 2: Hildago

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Redesign 3: Stella

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Solidworks of Hildago

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Future designs and contributions • May consider other designs after thinking about the

logistics •  Incorporating better battery life • Smaller fuel cell • Soldier feedback

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Wheelchair Redesign • Current project in conjunction with Children’s Healthcare

of Atlanta •  Improving a wheelchair for children with special needs that are

mostly immobile, but not paralyzed •  Design specifications include: ability to rise and fall to heights of

different tables, to be lightweight, affordable, and foldable

•  The upcoming design is the first iteration of a project over the next coming months •  Will be in production for the upcoming months

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Drawing: Redesign 1

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Drawing: Redesign 2

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Solidworks of Redesign 2: First iteration 57

•  Folds in half •  3 wheels for a sporty and youthful aesthetic •  Raises from the back using concentric cylinders

Video of the Wheelchair in Solidworks

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Jordan Garth portfolio

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