JOINT PROJECT WITH RHODE ISLAND COLLEGE DEPARTMENT OF PHYSICAL SCIENCES JULY 13- 17, 2009...
-
date post
21-Dec-2015 -
Category
Documents
-
view
214 -
download
0
Transcript of JOINT PROJECT WITH RHODE ISLAND COLLEGE DEPARTMENT OF PHYSICAL SCIENCES JULY 13- 17, 2009...
JOINT PROJECT WITH RHODE ISLAND COLLEGEDEPARTMENT OF PHYSICAL SCIENCES
JULY 13- 17, 2009
Interaction of o-Xylylenebis(triphenylphosphonium bromide)
and DNA
Jennifer Andrade, Renae Miozzi, Ellen Rounds
Exeter-West Greenwich Exeter-West Greenwich Regional High SchoolRegional High School
Biotech IIIBiotech III
O-XYLYLENEBIS(TRIPHENYLPHOSPHONIUM BROMIDE)
“o-XYLY”
•Classification: Benzylic Phosphonium Salt•Molecular Weight: 788.53•Melting point: > 300 C �•Appearance
SolidOff-white or grey
•Not known to be cytotoxic in humans(Not a Hazard) •Hygroscopic
Formula: Formula: C6H4[CH2P(C6H5)3Br]2
Benzylic Phosphonium Salts
Anti-trypanonosomal effects (Trypanicide)• used to control Trypanosoma gambiense and T rhodesiense – insect vectors• work as an acetylcholinesterase inhibitor•Toxic to parasite only
Latent Thermal Indicator• used in epoxy and paint by NASA
Anti-fungal effects (Fungicide)• used to control plant fungi in agriculture
Electrophoresis ResultsLane 5 Control (X)
BP Measurement for X
Distance (mm) BP28 5,00036 2,000
E (DNA with Hind III enzyme )Eb – with o-Xyly
X ( DNA without Hind III enzyme )Xb – with o-Xyly
Eb
10µl
Eb
15 µl
X 0µ
l
Xb
5µl
Xb
10µl
Xb
15µl
Eb
5µl
E 0 µ
l
BP Measurement for E
Distance (mm) BP31 4,000
Lane 1 Control (E)
Plasmid DNA with Hind III enzyme, with and without o-Xyly
Uncut plasmid with and without o-Xyly
Mas
s of
DN
A fr
agm
ents
(ng)
Base Pairs from Hind III Ladder (BP)
HyperChem Location Before
This is an illustration of our compound before optimization and it shows the location in the Major Groove.
The Distance between 27 C to atom 1H5M = 4.6743 ÅFrom 26 C to atom 90 C = 15 Å
From 26 C to atom 239 C = 4.6 Å
Energy Level-6675.93671Kcal/molGradient Value-285.526871
HyperChem Interactions:
• The phosphate bonds repositioned themselves to accommodate a lower energy, but the DNA molecule does not appear to be altered.
• The DNA and O-XYLY did not bond in a visible manner, but our data did prove a bond.
HyperChem(Steepest Descent) Locations After
The compound and DNA did not show any signs of physically bonding although the energy levels were decreased
The Distance between 27 C and 1H5M = 5.01036Å (increase)
Energy Level=264.62Gradient Value-0.099511
HyperChem Results:
Before Optimization After Optimization
Energy=1791.844854 Kcal/molGradient Value= 260.335489
Energy= -300.682007 Kcal/molGradient Value= 0.09384
Distance Between 28 Carbon and 57 Carbon = 20.6344Å
Distance Between 28 Carbon and 57 Carbon= 20.4114Å
Discussion
• Our Experimental results did not compare with our predictions because we went into this project hoping to see a significant reaction between the compound and Native DNA.
• Experimental changes with electrophoresis• Further Research- electrophoresis with higher
concentration and running Smart Spec™ in reverse temperatures.