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THE J O U R N A L OF

IMMUNOLOGY

V()LUME 111

NUMBERS 4 TO 6

BALTIMORE, MD. 21202

1973

JOIMA3

COPYRIGHT ~) 1973, BY THE WILI.I~S & WILKINS COMPANY

NUMBER 1, JULY 1973

CELLULAR IMMUNOLOGY

Regulation of Antibody Responses in Vitro. V. Effect of Carrier-Specific Helper Cells on Generation of Hapten-Specific Memory Cells of Different Immunoglobulin Classes. Tadamitsu Kishimoto and Kimishige Ishizaka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

Enhanced Antibody Response of Mice to Polyinosinic Polycytidylic Acid by Antithymocyte Serum and Its Age-Dependent Loss in NZB/W Mice. Thomas M. Chused, Alfred D. Steinberg, and Leroy M. Parker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

Antigen-Induced Proliferation of Guinea Pig Lymphocytes in Vitro: Obligatory Role of Macrophages in the Recognition of Antigen by Immune T-Lymphocytes. James A. Waldron, Jr., Robert G. Horn, and Alan S. Rosenthal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

Alternative Potentiat ing and Inhibitory Effects of GVH Reaction on Formation of Antibodies Against a Thymus-lndependent Polysaccharide (SIII). Patricia Byfield, George H. Christie, and James G. Howard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

Antigen-T Lymphocyte Interactions: Inhibition by Cytoehalasin B. Christopher S. Henney and J. Eric Bubbers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

Development of Primary 3,M, ~G, and 3'A Anti -TNP Plaque-Forming Cell Responses in Vitro. Harry G. Bluestein and Carl W. Pierce . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130

Cellular Requirements for Development of Primary Anti-Hapten Antibody Responses in Vitro. Harry G. Bluestein and Carl W. Pierce . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137

Studies on Rabbit Lymphocytes in Vitro. XVI. Restimulation of Antiallotypically Stimulated and Blocked Cultures. Stewart Sell, Jennifer A. Lowe, and P. G. H. Gell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144

Effect of Irradiation and Appendicostomy on Appendix Structure and Responses of Appendix Cells to Mitogens. Hildur E. Blythman and Byron H. Waksman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171

In Vitro IgM Antibody Synthesis by Human Tonsil-Derived Lymphoeytes. John L. Sloyer, Robert W. Veltri, and Philip M. Sprinkle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 183

IMMUNOCHEMISTRY

Morphologic, Chemical, and Immunologic Studies of Amyloid-Like Fibrils Formed from Bence Jones Proteins by Proteolysis. R .P . Linke, D. Zucker-Franklin, and E. C. Franklin . . . . . . . . . . . . . . . . . . . . . 10

The Formation of Amyloid-Like Fibrils in Vitro from Benee Jones Proteins of the VXI-Subclass. R .P . Linke, F. W. Tischendorf, D. Zucker-Franklin, and E. C. Franklin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

Molecular Restriction of Anti-DNP Antibodies Induced by Dinitrophenylated Type III Pneumococcus. Paul C. Montgomery and Jack H. Pincus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

The Role of Antigenic Determinants in the Control of IgM and IgG Antibody Responses to Denatured DNA. Ruben Gruenewald and B. David Stollar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106

Denaturation of Lysozyme by Freund's Complete Adjuvant. Robert J. Scibienski . . . . . . . . . . . . . . . . . . . . . . 114 Immune Response of Mice to Nucleic Acids: Strain-Dependent Differenees in Magnitude and Class of

Antibody Production. B. David Stollar, Sara Fuchs, and Edna Mozes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 Complement: Optimal Reaction Conditions for Guinea Pig C4. William D. Linscott . . . . . . . . . . . . . . . . . . . 189 Complement: A Reversible Temperature-Dependent Alteration in the Reactivity of EAC1 with C4. Wil-

liam D. Linscott . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 200 Lymphocyte Receptor Movement Induced by Sheep Erythrocyte Binding. Robert F. Ashman . . . . . . . . . . 212 Intestinal Uptake of Macromolecules. II. Effect of Parenteral Immunization. W. A. Walker, K. J.

Isselbacher, and K. J. Bloch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 221 The Relationship of Binding Affinity to b4 Allotype in Rabbit Anti-Dinitrophenyl Antibody. Silvia

Fontana, Ciro Manzo, and Serafino Zappacosta . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 227

IMMUNOGENETICS AND TRANSPLANTATION

The Importance of Chimerism in Maintaining Tolerance of Skin Allografts in Mice. David M. Lubaroff and Willys K. Silvers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

Genetics of the Immune Response. II. Inheritance of Antibody Specificity to Lipopolysaccharides in Mice. Raimund Di Pauli . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

Genetic Control of Immune Response toward the Loop Region of Lysozyme. Elchanan Maron, Howard I. Scher, Edna Mozes, Ruth Arnon, and Michael Sela . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101

i i i

iv CONTENTS [VOL. 111

IMMUNOPATHOLOGY

Immunologic Release of Histamine and Slow Reacting Substance of Anaphylaxis from Human Lung. V. Effects of Prostaglandins on Release of Histamine. Alfred I. Tauber, Michael Kaliner, Daniel J. Stechsehulte, and K. Frank Austen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Lysosomal Enzyme Release from Human Leukocytes: Mediation by the Alternate Pathway of Comple- ment Activation. Ira M. Goldstein, Melehiorre Brai, Abraham G. Osier, and Gerald Weissmann . . . . 33

Myelination Inhibition Factor: Its Absence in Sera from Subhuman Primates Sensitized with Myelin Basic Protein. Frederick J. Sell, Helene C. Rauch, Elizabeth Reboz Einstein, and Ann E. Hamilton 96

The Role of the Inflammatory Response of Skin and Lymph Nodes in the Induction of Sensitization to Simple Chemicals. Mitchell H. Friedlaender, Francis V. Chisari, and Harold Baer . . . . . . . . . . . . . . . . 164

Hypersensitivity to Ascaris Antigens. VIII. Characterization of a Highly Purified Allergen. Rabia Hussain, Susan M. Bradbury, and Gill Strejan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 260

TUMOR IMMUNOLOGY

Effects of Pyran Copolymer on Leukemogenesis in Immunosuppressed AKR Mice. Mart in S. Hirsch, Paul H. Black. Maw' L. Wood, and Anthony P. Monaco . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

VIRAL AND MICROBIAL IMMUNOLOGY

Immune Response of the Human Respiratory Tract. I. Immunoglobulin Levels and Influenza Virus Vaccine Antibody Response. R .H. Waldman. P. F. Jurgensen, G. N. Olsen, R. Ganguly, and J. E. Johnson, III . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

Intracellular Localization of Mammalian Type C Virus Species-Specific (gs-1) and Interspecies-Specific (gs-3) Antigenic Determinants with the Indirect lmmunoperoxidase Technique and Light Microscopy. Anthony Girardi, Berge Hampar, Konrad C. Hsu. Stephan Oroszlan, Elizabeth Hornberger, Gary Kel- loft, and Raymond V. Gilden . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152

Induction of Systemic Delayed Hypersensitivity during Experimental Viral Infection of the Respiratory Tract with a Myxovirus or Paramyxovirus. Roger E. Wetherbee . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157

Immunoglobulin Response in Experimental Infection with Cell Free and Cell Associated Marek's Disease Virus. R .M. Jakowski. T. N. Fredrickson, and R. E. Luginbuhl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 238

Cross-Reactions among Group A Streptococci. IV. Extraction, Separation and Purification of Two Protective Antigens of Type G~ Cocci. T. Timothy" Myoda. Grove G. Wiley, and Pauline N. Bruno . . 249

COMMUNICATIONS

Position of the Selective Peptic Cleavage of a Human ~-Chain between the V and C Domains. Byeong Kuk Seon, Allan L. Grossberg, Oliver A. Roholt. and David Pressman . . . . . . . . . . . . . . . . . . . . . . . . . . . 269

Immunoregulatory a-Globulin: Failure to Inhibit Antibody Response When Administered after Antigen Exposure. A.H. Glasgow, J. O. Menzoian. S. R. Cooperband, R. B. Nimberg, K. Schmid, and J. A. Mannick . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 272

T Cells Can Bind Antigen via Cytophilic IgM Antibody Made by B Cells. Susan R. Webb and Max D. Cooper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 275

Water Buffalo Blood Groups. IV. Antigenic Determinant Located Beneath the Surface of Red Cells. D. Iannelli. T. M. Bettini, and U. Oreste . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 278

Serologic Assay of HL-A Antigens in the Presence of Detergents. Jeffrey R. Dawson, Sandra S. Shasby, and D. Bernard Amos . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 281

Protection of Adult Mice Against Tumor Challenge by Immunization with Irradiated Adult Skin or Embryo Cells. Aaron Bendich, Ellen Borenfreund, and Elliott H. Stonehill . . . . . . . . . . . . . . . . . . . . . . . 284

SPECIAL ARTICLE

Abstracts of the Fifth International Complement Workshop, Hotel Del Coronado, Coronado, California, February 23-25. 1973 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286

NUMBZR 2, AUGUST 1973

CELLULAR IMMUNOLOGY

Induction of Tolerance to a Hapten. II. Maintenance and Escape of the Tolerant State to TNP. John M. Fidler and Edward S. Golub . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 317

Mitogenicity of Bacterial Endotoxins: Characterization of the Mitogenic Principle. Duane L. Peavy,

1973] CONTENTS v

Joseph W. Shands, Jr., William H. Adler, and Richard T. Smith . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 352 Studies of the Mechanism of Lymphocyte-Mediated Cytolysis. IV. Specificity of the Histamine Receptor

on Effector T Cells. Marshall Plaut, Lawrence M. Lichtenstein, Elizabeth Gillespie, and Christo- pher S. Henney . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389

Cooperative Effects in Antibody Formation Produced by Hapten-Speeific Delayed Sensitivity. Nabit Hanna and Sidney Leskowitz . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 410

The Spontaneous Induction of Anamnestic Antibody Synthesis in Lymph Node Cell Cultures Many Months after Primary Immunization. John G. Tew, Colin H. Self, Weldon W. Harold, and Abram B. Stavitsky . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 416

Proliferation of Stimulated Human Peripheral Blood Lymphocytes: Preferential Incorporation of Concanavalin A By Stimulated Cells and Mitogenic Activity. Richard M. Pauli, Louis DeSalle, Patrick Higgins, Earl Henderson, Allen Norin, and Bernard Strauss . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 424

Absence of Rosette-Forming Cells in Delayed Hypersensitive Agammaglobulinemic Chickens. G .A . Theis, F. I. Weinbaum, and G. J. Thorbecke . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 457

A Sensitive Microassay System for Lymphocyte-Mediated Cytotoxicity Induced by PHA and PPD. No- buyuki Amino and Leslie J. Degroot . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 464

Human Skin Responses to Products of Concanavalin A-Activated Lymphocytes. Helgi Valdimarsson and Nicholas J. Gross . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 485

The Immune Response against Hapten-Autologous Protein Conjugates in the Mouse. IV. Thymus Dependency of the Primary Response to Hapten-Autologous Albumin Conjugates. Bent Rubin, Klaus Hiesche, Volker Schirrmacher, and Hans Wigzell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 492

Immune Responses i n V i t r o . VIII. Analysis of Cell Clusters. John A. Mclntyre and Carl W. Pierce . . . . . . 512 Delayed Hypersensitivity to Beryllium Compounds. James J. Marx, Jr., and Robert Burrell . . . . . . . . . . . 590 Subpopulations of Human Lymphocytes Defined by/~-Microglobulin. Michael W. Fanger and George

M. Bernier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 609 Immunity to Chlamydial Infections of the Eye. Ill. Presence and Duration of Delayed Hypersensitivity to

Guinea Pig Inclusion Conjunctivitis. Ronald R. Watson, A. Bruce MacDonald, Edward S. Murray, and Farrokh Z. Modabber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 618

IMMUNOCHEMISTRY

Characterization of an Immunosuppressive Factor Present in Mouse Serum. Bruce Veit and J. Gabriel Michael . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 341

Synthesis, Assembly and Secretion of ~,-Globulin by Mouse Myeloma Cells. V. Balanced and Unbalanced Synthesis of Heavy and Light Chains by IgG-Producing Tumors and Cell Lines. Reuben Baumal and Matthew D. Scharff . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 448

Immunologic Studies on Drug Addiction. I. Antibodies Reactive with Methadone and Their Use for Detection of the Drug. Chi-Tan Liu and Frank L. Adler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 472

Role of Intracellular and Extracellular Cationic Composition in Immune Lysis of Mammalian Eryth- rocytes. Agustin P. Dalmasso, Elda B. Giavedoni, Rosalia Lelchuk, and Maria M. E. deBracco . . . . 527

Interactions between Human C5, C6, and C7 and Their Functional Significance in Complement-Depend- ent Cytolysis. Carlos M. Arroyave and Hans J. Miiller-Eberhard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 536

A New Serum Factor in Normal Rabbits. IX. Association with Group b Immunoglobulin Allotypic Specificities. K. Hagen, S. S. Kulkarni, and W. J. Mandy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 546

Use of an Aqueous Polymer Phase System as an Assay for Antibodies to E s c h e r i c h i a c o l i Alkaline Phosphatase. R .T . Reese and H. P. Treffers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 624

Effects of Bursectomy and Thymectomy on Ontogeny of Fowl IgA, IgG, and IgM. Daniel Y. E. Perey and John Bienenstock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 633


Genetic Control of Immune Responses to Synthetic Polypeptides in Mice: Cellular Analysis of the Phenotypic Correction of the Ir-3 Gene Defect by Polyadenylic-Polyuridylic Acid. E. Mozes, G. M. Shearer, M. Sela, and W. Braun . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 439

Hyperacute Rejection of Skin Allografts in the Mouse by the Administration of Alloantibody and Rabbit Complement. R . A . P . Koene, P. G. G. Gerlag, J. F. H. M. Hagemann, U. J. G. van Haelst, and P. G. A. B. Wijdeveld . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 520

IMMUNOPATHOLOGY

On the Nature of the Presumed Receptor for IgE on Mast Cells. II. Demonstration of the Specific Binding of IgE to Cell-Free Particulate Preparations from Rat Peritoneal Mast Cells. Michael K. Bach and

vi CONTENTS [VOL. 111

John R. Brashler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 324 Characterization of IGE-Induced Systemic Anaphylaxis in the Rabbit. Lack of Correlation between the

Intravascular Release of Histamine and Anaphylactic Sensitivity. Marilyn Halonen, R. Neal Pinckard, and Aniko L. Meng . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 331

Mechanisms of Passive Sensitization. III. Number of IgE Molecules and Their Receptor Sites on Human Basophil Granulocytes. Teruko Ishizaka, Carmen S. Soto, and Kimishige Ishizaka . . . . . . . . . . . . . . . . 500

Immunogenicity and Arthritogenicity in the Rat of an Antigen from Mycobacterium tuberculosis Wax D. Toshitaka Koga and Carl M. Pearson . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 599

TUMOR IMMUNOLOGY

Allograft Immuni ty in the Mouse. I. Quantitat ion and Specificity of Cytotoxic Effector Cells after in Vitro Sensitization. H.R. MacDonald, R. A. Phillips, and R. G. Miller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 565

Allograft Immuni ty in the Mouse. II. Physical Studies of the Development of Cytotoxic Effector Cells from Their Immediate Progenitors. H.R. MacDonald, R. A. Phillips, and R. G. Miller . . . . . . . . . . . . 575


Pulmonary Host Defenses. I. Analysis of Protein and Lipids in Bronchial Secretions and Antibody Responses after Vaccination with Pseudornonas aeruginosa. Herbert Y. Reynolds and Russell E. Thompson . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 358

Pulmonary Host Defenses. II. Interaction of Respiratory Antibodies with Pseudomonas aeruginosa and Alveolar Macrophages. Herbert Y. Reynolds and Russell E. Thompson . . . . . . . . . . . . . . . . . . . . . . . . . . . 369

Recognition of Hepatitis B Antigen Determinants by a Double Label Radioimmunoassay: A Sensitive Means of Subtyping Hepatitis B Antigen. Richard D. Aach, Edwin J. Hacker, and Charles W. Parker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 381

Protection against Enteric Bacterial Infection by Secretory IgA Antibodies. Ernest S. Fubara and Rolf Freter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 395

Preferential Radioassay of Influenza-Specific 7S (IgG} Over 19S (IgM) Class of Antibodies. Harry Daugharty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 404

Treatment of E. coli and Klebsiella Baeteremia in Agranuloeytie Animals with Antiserum to a UDP-Gal Epimerase-Deficient Mutant . Elizabeth J. Ziegler, Herndon Douglas, James E. Sherman, Charles E. Davis, and Abraham I. Braude . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 433

A Latex Agglutination Test for Measuring Antibodies to Streptococcal Mucopeptides. Berno Heymer, Walter Schachenmayr, Burkhard B~ltmann, Reinhard Spanel, Otto Haferkamp, and Willard C. Schmidt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 478

Application of Electroimmunodiffusion and Crossed Electroimmunodiffusion to the Comparative Serology of A Microorganism (Histoplasma capsulotum). George H. Sweet and Donna E. Wilson .. 554

COMMUNICATIONS

Heterologous Adoptive Cutaneous Anaphylaxis: A Method lor Detecting Reaginic Antibody Formation by Cells of the Mouse. L .S . Kind and B. Macedo-Sobrinbo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 638

A Third Non-Adherent Cell Required for the in Vitro Immune Response to Sheep Erythrocytes. David W. Talmage and Helen Hemmingsen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 641

Interferon Induction in New Zealand Black Mice by Murine Leukemia Virus. Benjamin V. Siegel, McKay Brown, and Jane I. Morton . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 644

Homocytotropic Autoantibodies in Rabbits Subjected to Cryosurgery. Clelia M. Riera, Maria G. Faillaci, and Carlos Yantorno . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 647

Suppressor Cells in the Graft-Versus-Host Reaction. John A. Hardin, Thomas M. Chused, and Alfred D. Steinberg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 650

Retention of Graft-vs-Host Activity in Non-Adherent Spleen Cells after Depletion of Cytotoxic Activity by Incubation on Allogeneic Target Cells. Michael G. Mage and Louise L. McHugh . . . . . . . . . . . . . . . 652

NtJ~mER 3, SEVrEMBER 1973

CELLULAR IMMUNOLOGY

Effects of Sublethal Irradiation on the Plaque-Forming Cell Response in Mice. Jon R. Schmidtke and Frank J. Dixon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 691

T-Cell Deficiency in Mouse Allogeneic Radiation Chimeras. Paul Urso and Nazareth Gengozian . . . . . . . 712

19731 CONTENTS vii

Regulation of Antibody Response in Vitro. VI. Carrier-Specific Helper Cells for IgG and IgE Antibody Response. Tadamitsu Kishimoto and Kimishige Ishizaka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 720

Differentiation of Lymphoid Cells: The in Vitro Emergence of Immunoglobulin M-Secreting Cells. Danio H. Zimmerman and Milton Kern . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 761

Characteristics of a Cytotoxin ("Lymphotoxin") Produced by Stimulation of Human Lymphoid Tissues. James B. Peter, Joan A. Stratton, Kerstin E. Stempel, David Yu, and Claire Cardin . . . . . . 770

Cellular Events in Tolerance. I. Failure to Demonstrate Activation of Lymphocytes, Blocking Factors, or Suppressor Cells during the Induction of Tolerance to a Soluble Protein. David W. Scott . . . . . . . . . . 789

Immunoglobulin Production in Synchronized Cultures of Human Hematopoietic Cell Lines. II. Variation of Synthetic and Secretion Activities during the Cell Cycle. Shinichiro Watanabe, Yasuo Yagi, and David Pressman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 797

The Cellular Basis of Cross-Tolerance. Thoman J. Ruben, Jacques M. Chiller, and William O. Weigle .. 805 Antigen-Macrophage Interaction. I. Hapten-Specific Inhibition of Antigen Interaction with Macrophages

f rom Immune Animals. Benjamin E. Cohen, Alan S. Rosenthal, and William E. Paul . . . . . . . . . . . . . 811 Antigen-Macrophage Interaction. II. Relative Roles of Cytophilic Antibody and Other Membrane

Sites. Benjamin E. Cohen, Alan S. Rosenthal, and William E. Paul . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 820 Enumerat ion and Analysis of Antibody-Forming Cell Precursors in the Neonatal Mouse. Joan L. Press

and Norman R. Klinman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 829 Technical Aspects of the Rosette Tests Used to Detect Human Complement Receptor (B) and Sheep

Erythrocyte-Binding (T) Lymphocytes. Nelson F. Mendes, Marina E. A. Tolnai, Nelson P. A. Silveira, Richard B. Gilbertsen, and Richard S. Metzgar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 860

The Homing Pat tern of Thymus-Derived Lymphocytes in Calf and Neonatal Mouse Peyer's Patches. Byron H. Waksman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 878

Reconstitution of T Cell-Depleted Spleen Cell Populations by Factors Derived from T Cells. III. Mechanism of Action of T Cell-Derived Factors. R .M. Gorczynski, R. G. Miller, and R. A. Phillips 900

The Number of Reactive Cells in Mouse Lymphocyte Cultures Stimulated by Phytohemagglutinin, Concanavalin A or Histocompatibility Antigen. G. Jones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 914

Antigen Stimulation of SH Thymidine Incorporation by Subpopulations of Human Peripheral Blood Cells. Seth A. Eisen, Leon R. Lyle, and Charles W. Parker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 962

IMMUNOCHEMISTRY

Purification of the Human Complement Protein Clq by Affinity Chromatography. Carlos R. Sledge and David H. Bing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 661

The Role of Complement in Human Immediate Hypersensitivity: Evidence against Involvement of the Alternate Pathway of Complement Activation. J. Andrew Grant and Lawrence M. L ich tens te in . . . 733

Detection of Idiotypic Determinants on Human Monoclonal Proteins with Specific Rabbit Antisera and Hemagglutination-Inhibition. J. Vivian Wells, An-Chuan Wang, Charles T. Thornsvard, Melvin S. Schanfield, Jan F. Bleumers, and H. Hush Fudenberg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 841

Cross-Reactions of Additional Extracellular Polysaccharides of Klebsiella. Michael Heidelberger and Guy G. S. Dutton . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 857

Characterization and Antibody-Binding Capacity of Streptococcal Group A Carbohydrate Haptens. Stanford T. Shulman and Elia M. Ayoub . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 868

Antigenicity of Formaldehyde-Inactivated Staphylococcal Enterotoxin B. John R. Warren, Leonard Sporo, and Joseph F. Metzger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 885

Extraction of Complement Inhibitory Factors from the Erythrocytes of Non-Human Species. E . M . Hoffman and H. M. Etlinger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 946


The Responsiveness of Tolerant Lymphocyte Populations after Transfer to Normal Recipients. D .D. McGregor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 672

Heart Autoantibodies in Mice: Genetic, Bacterial, and Age Determinants. John H. Schwab and Roger R. Brown . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 682

Expression of the a, x, and y Variable Region Genes of Heavy Chains among IgG, IgM, and IgA Molecules of Normal and a Locus Allotype-Suppressed Rabbits. Byung Suk Kim and Sheldon Dray . . . . . . . . . . 750

Extraction and Purification of Soluble HL-A Antigens from Exhausted Media of Human Lymphoid Cell Lines. Michele A. Pellegrino, Anna Pellegrino, Soldano Ferrone, Barry D. Kahan, and Ralph A. Reisfeld . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 783

viii CONTENTS [VOL. 111

IMMUNOPATHOLOGY

Competitive Effect of Homologous IgG or Fc in Sensitization of Rabbits for PCA Mediated by Reaginic Antibody. Roberto Revoltella and Zoltan Ovary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 698

The Skeletal Muscle-Binding Site of Antistriated Muscle Antibody in Myasthenia Gravis: An Electron Microscopic Immunohistochemical Study Using Peroxidase-Conjugated Antibody Fragments. J .R. Mendell, J. N. Whitaker, and W. K. Engel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 847

A Neutrophil Immobilizing Factor Derived from Human Leukocyte. II. Specificity of Action on Polymorphonuclear Leukocyte Mobility. Edward J. Goetzl, Irma Gigli, Stephen Wasserman, and K. Frank Austen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 938

Regulation of Homocytotropic Antibody Formation in the Rat. VIII. An Antigen-Specific T Cell Factor That Regulates Anti-Hapten Homocytotmpic Antibody Response. Tomio Tada, Ko Okumura, and Masaru Taniguchi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 952

TUMOR IMMUNOLOGY

Further Evidence for the Involvement of Thymus-Processed Lymphocytes in Syngeneic Tumor Cell Cytolysis. Fernando Plata, Elisabeth Gomard, Jean-Claude Leclerc, and Jean-Paul Levy . . . . . . . . . 667

Alterations in Lymphocyte Populations in Tumorigenesis. I. Lymphocyte Trapping. Marion M. Zatz, Abraham White, and Allan L. Goldstein . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 0 6

RNA-Mediated Immunologic Depression. M . E . M . Cotmerauer, Lia Rumi, Fortuna Saal, Christiane Dosne Pasqualini, and S. L. Rabasa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 743

Separation of Lymphoc~es from Disaggregated Mouse Malignant Neoplasms by Sedimentation in Gradients of Ficoll in Tissue Culture Medium. Judy G. Zettergren, Douglas E. Luberoff, and Thomas G. Pretlow, II . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 836

Studies of MOPC 104E Plasmacytoma Cells Forming Rosettes with Autologous and Syngeneic Erythrocytes. Raymond N. Hiramoto and Vithal K. Ghanta . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 893

Selective Cytotoxicity of Hapten-Substituted Cells with an Antibody-Enzyme Conjugate. Gordon W. Philpott, William T. Shearer, Richard J. Bower, and Charles W. Parker . . . . . . . . . . . . . . . . . . . . . . . . . . . 921

Selective Cytotoxicity in a Hapten-Substituted Cell Culture Model System. Gordon W. Philpott, Richard J. Bower, and Charles W. Parker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 930


I n V i t r o Assessment of Cellular Immunity to Rauscher Leukemia Virus. Richard F. Mortensen, Walter S. Ceglowski, and Herman Friedman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 657

COMMUNICATIONS

Reversibility of the Immunodepression Due to Ehrlich Ascites Carcinoma. Paoli Mocarelli, Maria Luisa Villa, Gianni Garotta, Carla Porta, Giuseppe Bigi, and Enrico Clerici . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 973

Graft-vs-Host Reaction with an H-2 Mutant. J. Klein and I. K. Egorov . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 976 Immunologic Responses in the Gastrointestinal Tract of the Guinea Pig. I. Characterization of Peyer's

Patch Cells. Douglas M. Levin, David L. Rosenstreich, and Herbert Y. Reynolds . . . . . . . . . . . . . . . . . . 980 Xenogeneic Reactivity of Human Fetal Lymphocytes. Tuula Asantila, Tapani Sorvari, Toivo Hirvonen,

and Paavo Toivanen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 984

NU~mER 4, OCTOB~B 1973

Presidential Address. Dan H. Campbell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 989

CELLULAR IMMUNOLOGY

The Role of Thymus-Derived Lymphoeytes in the i n V i t r o Activation of Macrophages to Kill L i s t e r i a

m o n o c y t o g e n e s . James L. Krahenbuhl, Leon T. Resenberg, and Jack S. Remington . . . . . . . . . . . . . . . 992 Production of Functional T Cells after Treatment of Bone Marrow with Thymic Factor. Harold C.

Miller, Susan K. Schmiege, and Allyn Rule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1005 Role of Cyclic AMP in Mitogen Induced Transformation of Human Peripheral Leukocytes. R.

Krishnaraj and G. P. Talwar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1010 The Proliferative and Anamnestic Antibody Response of Rabbit Lymphoid Cells i n V i t r o . II. Effect of

Passive Antibody on Immunologic Memo~' in Lymph Nodes Contralateral to the Site of Antigen Injection. G. Jeanette Thorbecke and Melvin K. Bell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1043

Effect of Locally Administered Endotoxin on Regenerating Appendix Structures and Responses of Appendix Cells to Mitogens. Hildur E. Blythman and Byron H. Waksman . . . . . . . . . . . . . . . . . . . . . . . 1081

1973] CONTENTS ix

Lymphocyte Phytohemagglutinin Responsiveness, Immunoglobulins and Autoantibodies in Aging Humans. H .M. Hallgren, C. E. Buckley, III, V. A. Gilbertsen, and E. J. Yunis . . . . . . . . . . . . . . . . . . . 1101

Stimulation of 7-Globulin Synthesis and Specific Antibody Production by a Factor Released by Activated Lymphocytes. Moshe Rosenthal, Peter Stastny, and Morris Ziff . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1119

Early Biochemical Alterations Induced by Lymphotoxin in Target Cells. Werner Rosenau, Melvin L. Goldberg, and Gertrude C. Burke . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1128

Carrier Independent T Cell Helper Effects in Antigenic Stimulation. Robert A. Doughty and Norman R. Klinman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1140

Transient Appearance of Cells Secreting Antibody of Different Specificities after Immunizat ion of Mice with Trinitrophenylated Erythrocytes. Jacques Couderc, Christian Bleux, Jean-Louis Birien, and Panayotis Liacopoulos . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1155

Antigen-Induced Deoxyribonucleic Acid Synthesis in Mouse Lymphocytes. I. The Nature and Specificity of Lymphocyte Activation by Hemocyanin and Dinitrophenyl Carrier Conjugates. David P. Osborne, Jr., and David H. Katz . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1164

Antigen-Induced Deoxyribonucleic Acid Synthesis in Mouse Lymphocytes. II. Analysis of the Cell Populations Required for and Responding to Antigen Stimulation in Vitro. David P. Osborne, Jr., and David H. Katz . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1176

Studies on Early Events in the Differentiation of Antibody-Producing Cells. I. Patterns of Deoxyribonu- cleic Acid Replication in Antigen-Stimulated Mouse Spleen Cells. Neomi Moav, Sara Smolinsky, Boaz Moav, and Asher Frensdorff . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1183

Regulation of Antibody Response in Vitro. VII. Enhancing Soluble Factors for IgG and IgE Antibody Response. Tadamitsu Kishimoto and Kimishige Ishizaka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1194

Regulation of the Immune Response. IV. Antibody-Mediated Suppression of the Immune Response to Haptens and Heterologous Erythrocyte Antigens in Vitro. John W. Kappler, Andr~ van der Hoven, Umarani Dharmarajan, and Michael Hoffmann . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1228

Immunoglobul in Biosynthesis by Normal and Leukemic H u m a n Peripheral Blood Lym- phocytes. Kenneth M. Nies, M. Anne Oberlin, John C. Brown, and Michael S. Halpern . . . . . . . . . . . 1236

The Mechanism of a Hapten-Specific Helper Effect in Mice. Charles A. Janeway, Jr . . . . . . . . . . . . . . . . . . . 1250

IMMUNOCHEMISTRY

Effect of Cortisone Therapy on Serum Complement Components. John P. Atkinson and Michael M. Frank . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1061

Synthesis of IgG, IgA, IgM by Chicken Tissues: Immunofluorescent and ~4C Amino Acid Incorporation Studies. John Bienenstock, Jack Gauldie, and D. Y. E. Perey . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1112

Demonstration of Antibodies to Deoxyribonucleic Acid by Counterelectrophoresis. A. Klajman, Ruth Farkash, and B. D. Myers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1136

B2-Microglobulin in Human Blood Cells. P .E . Evrin and H~kan Pertoft . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1147 Detection of Antibodies and Soluble Antigen-Antibody Complexes by Precipitation with Polyethylene

Glycol. W.D. Creighton, P. H. Lambert, and P. A. Miescher . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1219 Immunoglobulins in the Developing Amphibian, Rana calesbeiana. Carolyn L. Geczy, Patrick C. Green,

and Lisa A. Steiner . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1261


Genetic Control of the Immune Response in Guinea Pigs to the Known Sequence Polymer (Tyr-Glu-Ala- Gly)n. Paul H. Maurer, Gerald Odstrchel, and Carmen F. Merryman . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1018

Selective Loss of H-2 Antigenic Reactivity after Chemical Modification. Samuel J. Pancake and Stanley G. Nathenson . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1086

Anti-AUoantibodies: A De Novo Product. Hans Binz, Hansruedy Ramseier, and Jean Lindemann • 1108 Relationship between Proliferative and Effector Phases of the Mixed Lymphocyte Reaction and

Graft-versus-Host Reaction. Michael Howe, Lee Berman, and Lewis Cohen . . . . . . . . . . . . . . . . . . . . . . 1243

IMMUNOPATHOLOGY

Antinucleolar Antibodies in Human Sera. Jacob L. Pinnas, James D. Northway, and Eng M. Tan . . . . . . 996 Failure to Obtain Histamine Release from Rat Mast Cells Exposed to Human Allergic Serum and Specific

Antigen or IgE Myeloma Protein and Anti-IgE. Marshall Plaut, Lawrence M. Lichtenstein, and Kurt J. Bloch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1022

The Pathogenesis of Corneal Inflammation Due to Herpes Simplex Virus. I. Corneal Hypersensitivity to HSV Antigens in the Rabbit . Reberta L. Meyers and Thomas H. Pett i t . . . . . . . . . . . . . . . . . . . . . . . . . . 1031

X CONTENTS [VOL. 111

Antigen-Induced Inhibition of Experimental Allergic Encephalomyelitis. II. Studies in Guinea Pigs with the Small Rat Myelin Basic Protein. Robert H. Swanborg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1067

TUMOR IMMUNOLOGY

Thymus Function in Spontaneous Lymphoid Leukemia. I. Premature Leukemogenesis in "Young" Thymectomized Mice Bear ing"Old" Thymus Grafts. Hiroshi Nagaya . . . . . . . . . . . . . . . . . . . . . . . . . . . 1048

Thymus Function in Spontaneous Lymphoid Leukemia. II. I n V i t r o Response of "Preleukemic" and Leukemic Thymus Cells to Mitogens. Hiroshi Nagaya . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1052

Factors Involved in the Cytotoxicity of Normal Guinea Pig Serum for Cells of Murine Tumor TA3 Sublines Treated with Neuraminidase. R. Colin Hughes, Peter D. Palmer, and Barbara H. Sanford 1071

Association of Tumor and Histocompatibility Antigens in Sera of Lymphoma-Bearing Mice. Shigeyoshi Fujimoto, Ching Hong Chen, Edris Sabbadini, and Alec H. Sehon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1093

T Cell Surface Markers on Lymphoblasts from Acute Lymphocytic Leukemia. Luis Borella and Luisa Sen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1257


Purif icat ion of Mouse Interferon by Affinity Chromatography on Ant i - Interferon Globulin- Sepharose. Clifton A. Ogburn, Kurt Berg, and Kurt Paucker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1206

COMMUNICATIONS

Local Transfer of Delayed Hypersensitivity by T Lymphocytes. Adrian M. Jaffer, Gerald Jones, Earl J. Kasdon, and Stuart F. Schlossman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1268

Thymus Independent Stimulator Cells in the Mixed Lymphocyte Reaction. Michael R. Harrison . . . . . . 1270 Human Immunoglobulin A: Production of an Fc Fragment by an Enteric Microbial Proteolytic

Enzyme. Satish K. Mehta, Andrew G. Plaut, Nickolas J. Calvanico, and Thomas B. Tomasi, J r . . . . 1274 The i n V i t r o Response of Chicken B Cell Populations to Lipopolysaccharide. W.T. Weber . . . . . . . . . . . . . 1277 Use of Normal Mouse Serum to Detect Indirect Plaque-Forming Cells. Donald M. Silver and Henry J.

Winn . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1281 Comparative Chemical Study of a- and ~-Chains from a Single Patient (SC). B.-K. Seon, Y. Yagi, and

D. Pressman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1285 Spleen Cell Cytotoxicity in New Zealand Black Mice (NZB) with Autoimmune Disease. E .M. Liburd,

A. S. Russell, and J. B. Dossetor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1288 Specific Antigenicity of Thymic Reticuloepithelial Cells. Edward F. Potworowski, Adrien G. Borduas,

and Gilles Lussier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1292 Independent Movement of IgD and IgM Molecules on the Surface of Individual Lymphocytes. W. Knapp,

R. L. H. Bolhuis, J. Radk and W. Hijmans . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1295

NUMBER 5. NOVEMBEB 1973

CELLULAR IMMUNOLOGY

The Role of Humoral Factors in the Initiation of i n V i t r o Primary Immune Responses. III. Characteriza- tion of Factors that Replace Thymus-derived Cells. James Watson . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1301

Specific Heterologous Enhancement of Immune Responses. V. Isolation of a Soluble Enhancing Factor from Supornatants of Specifically St imulated and Allogenetically Induced Lymphoid Cells. Arnold S. Rubin, A. Bruce MacDonald, and Albert H. Coons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1314

Differentiation of Lymphoid Cells: Effect of Serum and Other Mitogonic Agents on the Selective Induction of Immunoglobulin M Secreting Lymph Node Cells in Tissue Culture. Daniel H. Zimmerman and Milton Kern . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1326

Immunoglobulin (Ig) Allotype Markers on Rabbit Lymphocytes: Separation of Cells Bearing Different Allotypes and Demonstration of the Binding of Ig to Lymphoid Cell Membranes. Patricia P. Jones, John J. Cebra, and Leonard A. Herzenberg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1334

Vitamin A: Adjuvant and Steroid Antagonist in the Immune Response. Benjamin E. Cohen and I. Ketman Cohen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1376

Induction of Cell-Mediated Immunity and Tolerance to Homologous Collagen in Guinea Pigs: Demonstration of Antigen-Reactive Cells for a Self-Antigen. George Senyk and Dov Michaeli . . . . . . 1381

Reaginic Antibody Formation in the Mouse. III. Collaboration Between Hapten-Specific Memory Cells and Carrier-Specific Helpers Cells for SecondaD' AntioHapten Antibody Formation. Hirokazu

1973] CONTENTS xi

Okudaira and Kimishige Ishizaka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1420 Reversible Inhibition of Lymphocyte-Mediated Cytolysis by Dimethyl Sulfoxide (DMSO). Gerald

Wolberg, Kay Hiemstra, Joseph d. Burge, and Robert C. Singler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1435 Complement Receptors on Cell Membranes. I. Evidence for Two Complement Receptors. Hidechika

Okada and Kusuya Nishioka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1444 Lymphocyte-Derived Eosinophil Chemotactic Factor. II. Studies on the Mechanism of Activation of the

Precursor Substance by Immune Complexes. Motomichi Torisu, Takeshi Yoshida, Peter A. Ward, and Stanley Cohen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1450

Long-term Survival of Mouse Thymic Lymphoid Cells i n V i t r o : Effect of Human Serum. "Keiko Ozato . . . 1459 Studies of Thoracic Duct Lymphocytes of Mice. III. Celt Surface Antigen Characteristics. Mark A.

Mandet, Toshitado Takahashi, and Lloyd J. Old . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1474 Presence of Impairment of Humoral Immunity in Nonadherent Spleen Cells of Old Mice. Margaret L.

Heidrick and T. Makinodan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1502 Lymphocytes in Peyer's Patches of the Mouse: Analysis of the Constituent Cells in Terms of Their

Capacities to Mediate Functions of Mature T and B Lymphocytes. David H. Katz and Daniel Y. E. Perey . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1507

Immune Responses i n V i t r o . IX: Role of Cell Clusters. John A. McIntyre and Carl W. Pierce . . . . . . . . . . . 1526 An Effector-Cell Independent Step in Target Cell Lysis by Sensitized Mouse Lymphocytes. Eric Martz

and Baruj Benacerraf . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1538 A Mathematical Model for the Quantitation of Tumor and Antibody-Forming Cell Populations within an

Animal's Body. Raymond N. Hiramoto, Vithal K. Ghanta, Jerry R. McGhee, and N. Marlene Hamlin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1546

Cellular Site of Action of Various Adjuvants in Antibody Responses to Hapten-Carrier Conjugates. To- shiyuki Hamaoka and David H. Katz . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1554

IMMUNOCHEMISTRY

A Light Chain Tyrosyl Sequence in the Antibody Site of a Rabbit Anti-p-Azobenzoate Antibody. 0. A. Reholt, B. Friedenson, G. Radzimski, and D. Pressman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1367

The Reaction of Zymosan with the Properdin System in Normal and C4-Deficient Guinea Pig Serum: Demonstration of C3- and C5-Cleaving Multi-unit Enzymes, Both Containing Factor B, and Acceleration of Their Formation by the Classical Complement Pathway. Volker Brade, Gerald D. Lee, Anne Nicholson, Hyun S. Shin, and Manfred M. Mayer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1389

Analysis of Antibody Populations to Oligo-Alanine Determinants. III. The Participation of Subclasses IgGl and IgG,a in the Antibody R e s p o n s e in Inbred Mice. Hubert Kolb and Eberhardt Weiler . . . . . . 1479

Analysis of Antibody Populations to Oligo-Alanine Determinants. IV. Functional Similarity of Variable Regions of IgG1 and IgG2a Antibodies. Hubert Kolb and Eberhardt Weiler . . . . . . . . . . . . . . . . . . . . . . . . 1487

Identification of J-Chain in Polymeric Immunoglobulins from a Variety of Species by Cross-Reaction with Rabbit Antisera to Human J-Chain. Kunihiko Kobayashi, Jean-Pierre Vaerman, Herv6 Bazin, Anne-Marie LeBacq-Verheyden, and Joseph F. Heremans . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1590


Cellular Studies of the Genetic Control of Immune Response toward the Loop Region of Lysozyme. Edna Mozes, G. M. Shearer, Elchanan Maron, Ruth Arnon, and Michael Sela . . . . . . . . . . . . . . . . . . . . . . . . . . . 1429

Genetic Control of Thymus-Derived Cell Function. III. DNA Synthetic Responses of Rat Lymph Node Cells Stimulated in Culture with Concanavalin A and Phytohemagglutinin. R. Michael Williams, Michael J. Moore, and Baruj Benacerraf . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1571

Genetic Control of Thymus-Derived Cell Function. IV. Mitogen Responsiveness and Mixed Lymphocyte Reactivity of Thymus Cells and Lymph Node Cells from Lewis and Brown Norway Rats. R. Michael Williams, Michael J. Moore, and Baruj Benacerraf . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1579

IMMUNOPATHOLOGY

Mechanisms of Endotoxin Tolerance. VIII. Specificity of Serum Transfer. Sheldon E. Greisman, Edward J. Young, and Bernard DuBuy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1349

Role of Ca ++ in Mast Cell Activation, Desensitization, and Histamine Release by Dextran. James H. Baxter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1470

Detection of Slow Reacting Substance of Anaphylaxis (SRS-A) in Plasma of Guinea Pigs During Anaphylaxis. Daniel J. Stechshulte, Robert P. Orange, and K. Frank Austen . . . . . . . . . . . . . . . . . . . . . 1585

xii CONTENTS [VOL. 111

TUMOR IMMUNOLOGY

Lymphocyte Populations of AKR/J Mice. I. Effect of Aging on Migration Patterns, Response to PHA and Expression of Theta Antigen. Marion M. Zatz, Allan L. Goldstein, and Abraham White . . . . . . . . . . . 1514

Lymphocyte Populations of AKR/J Mice. II. Effect of Leukemogenesis on Migration Patterns, Response to PHA, and Expression of Theta Antigen. Marion M. Zatz, Abraham White, and Allan L. Goldstein . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1519


Studies of Antibodies to Non-Type-Specific Antigens Associated with Streptococcal M Protein in the Sera of Patients with Rheumatic Fever. Edwin H. Beachey, Itzhak Ofek, and Alan L. Bisno . . . . . . . . . . . . 1361

A Surface Component in Group A, C, and G Streptococci with Non-Immune Reactivity for Immunoglobu- lin G. GSran Kronvall . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1401

Failure to Demonstrate a Humoral Immune Response to Scrapie Infection in Mice. David D. Porter, Helen G. Porter, and Nancy A. Cox . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1407

Evidence for a Macrophage-Mediated Effect of Poliovirus on the Lymphocyte Response to Phytohemag- glutinin. F . J . C . J . Soonti~ns and J. van der Veen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1411

Role of Humoral and Cell-Mediated Immunity on the Recovery of Chickens from Fowlpox Virus Infection. Chiharu Morita . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1495

An Agglutination-Flocculation Test for Rapid Detection of Hepatitis B Antigen. Zarah F. Kachani and David J. Gocke . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1564

COMMUNICATIONS

Endogenous Synthesis of Membrane b5 by Lymphocytes from Rabbits Recovering from b5 Allotype Suppression. Michael R. Harrison, Patricia P. Jones, and Rose G. Mage . . . . . . . . . . . . . . . . . . . . . . . . . . 1595

Alternate Complement Pathway: Elevation of Cobra Venom-Induced Hemolytic Activity in Sera of Tumor-Bearing Mice. Melchiorre Brai and Abraham G. Osier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1598

Thymus Dependence of Reaginic Antibody Formation in Mice. J. Gabriel Michael and I. Leonard Bernstein . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1600

Selective Activation of Chicken T Lymphocytes by Concanavalin A. Paavo Toivanen and Auli Toivanen . 1602 Relationship between Graft-vs-Host Reactivity and Possession of the High Leukemia Genotype hr/hr.

Valerie A. FAnson and David L. Gasser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1604 Tissue Typing: An Experimental Solution in the Chicken. Molten Simonsen . . . . . . . . . . . . . . . . . . . . . . . . . 1607 Characterization of Glass-Adherent Human Mononuclear Cells. Charles A. Koller, Gerald W. King,

Paul E. Hurtubise. Arthur L. Sagone, and Albert F. LoBuglio . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1610 Change in Susceptibility of C3H/HeJ Mice to LCM Virus Infection. Michael B. A. Oldstone and Frank

J. Dixon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1613 Intercellular Communication in Stimulated Human Lymphocytes. Gilberto M. Oliveira-Castro, Mar-

cello A. Barcinski and Samuel Cukierman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1616

NUMBER 6, DECEMBER 1973

CELLULAR IMMUNOLOGY

The Role of Thymus during the Induction of Tolerance of a Thymus-Dependent Antigen. Julia M. Phillips-Quagliata, Donald O. Bensinger, and Franco Quagliata . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1712

Migration Inhibition of Rat Peritoneal Cells Induced by Streptococcal Mucopeptides. Characteristics of the Reaction and Properties of the Mucopeptide Preparations. Berno Heymer, Biirkhard B(iltmann, Walter Schachenmayr, Rienhard Spanel, Otto Haterkamp, and Willard C. Schmidt . . . . . . . . . . . . . . . . 1743

Binding of Maclura pomifera Lectin to Rat Lymphoid Cells and Erythrocytes. Joe M. Jones and Joseph D. Feldman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1765

Simultaneous Expression of Hapten Binding and Antibody Secretion by NIP-Primed Lymph Node Cells Early in the Immune Response. Richard B. Bankert and Benjamin Wolf . . . . . . . . . . . . . . . . . . . . . . . . . 1790

Leukemia Virus-induced Immunosuppression. IX. Depression of Delayed Hypersensitivity and MIF Production after Infection of Mice with Friend Leukemia Virus. Richard F. Mortensen, Walter S. Ceglowski, and Herman Friedman . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1810

Rosette Formation by Guinea Pig Thymocytes and Thymus Derived Lymphocytes with Rabbit Red Blood Cells. Miguel J. Stadecker, Gene Bishop, and Henry H. Wortis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1834

Phagocytic Cells as Effectors in a Cell-Mediated Immunity System. Gunther Dennert and Edwin S. Lennox . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1844

1973] CONTENTS x i i i

Evaluation of Cell-Mediated Cytotoxic Reactivity against Tumor Associated Antigens with ~2~I-Iododeox- yuridine Labeled Target Cells. Robert K. Oldham and Ronald B. Herberman . . . . . . . . . . . . . . . . . . . . . 1862

Effect of Coneanavalin A on the Homing of Labeled T Lymphocytes. Ronald W. Gillette, Gall O. McKenzie, and Margaret H. Swanson . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1902

Systemic Immunity after Local Antigenic Stimulation of the Lymphoid Tissue of the Gastrointestinal Tract. Richard M. Rothberg, Sumner C. Kraft, and Suzanne M. Michalek . . . . . . . . . . . . . . . . . . . . . . . 1906

Production and Properties of Migration Inhibitory Factor and Interferon in the Circulation of Mice with Delayed Hypersensitivity. J .S . Youngner and S. B. Salvin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1914

IMMUNOCHEMISTRY

The Stoichiometry of J Chain in Human Secretory IgA. Michael S. Halpern and Marian Elliott Koshland . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1653

Hemolysis of Sheep Erythrocytes in Guinea Pig Serum Deficient in the Fourth Component of Complement. I. Antibody and Serum Requirements. Joseph E. May and Michael M. Frank . . . . . . . . . . . . . . . . . . . . 1661

Hemolysis of Sheep Erythrocytes in Guinea Pig Serum Deficient in the Fourth Component of Complement. II. Evidence tbr Involvement of C1 and Components of the Alternate Complement Pathway. Joseph E. May and Michael M. Frank . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1668

Fractionation of Antibodies to the Pneumococcal Polysaccharides by Affinity Chromatography. William C. Cheng, Kevin J. Fraser, and Edgar Haber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1677

Studies of Biological and Serologic Activities of Rabbit-IgG antibody Depleted of Carbohydrate Residues. Ralph C. Williams, Jr., C. Kirk Osterland, S. Margherita, Sei Tokuda, and Ronald P. Messner . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1690

Recombination of the Polypeptide Chains of A WaldenstrSm's IgM tha t Binds IgG. Ralph E. Schrohenloher and Jiri Mestecky . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1699

Serum Proteins Involved in Decay and Regeneration of Cobra Venom Factor-Dependent Complement Activation. Douglas T. Fearon, K. Frank Austen, and Shaun Ruddy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1730

Sequence Variations in b9 Light Chains as Potential V Region Genetic Markers. Allen L. Thunberg, Henry Lackland, and Thomas J. Kindt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1755

Isolation of Mucin from Human Submaxillary Secretions. M. Mansoor Baig, Richard J. Winzler, and Owen M. Rennert . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1826

Restricted Rabbit Antibodies: Amino Acid Sequences of Rabbit H Chains of Allotype a l , a 2 , and a3 in the Region 80 to 94. Jeane-Claude Jaton, Dietmar G. Braun, A. Donny Strosberg, Edgar Haber, and James E. Morris . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1838

Decreased Epinephrine Response of Adenylate Cyclase Activity of Lymphoid Cells from Immunodeficient Pituitary Dwarf Mice. Robert G. Kemp, Yung-Chen Huang, and Ren~ J. Duquesnoy . . . . . . . . . . . . . . 1855


Mapping the H-2-Linked Genes Governing, Respectively, the Immune Responses to a Glutamic Acid-Alanine-Tyrosine Copolymer and to Limiting Doses of Ovalbumin. Edward K. Dunham, Mart in E. Dorf, Donald C. Shreffler, and Baruj Benacerraf . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1621

Histoincompatibility Recognition Reaction by Irradiated or Mitomycin-Treated Parental Cells against Hybrid Spleen Cells Produces Hybrid Cell Proliferation and Illusion of Reaction of Hybrid against Parent. James C. Kennedy and J. Ampoe Ekpaha-Mensah . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1639

Expression oi HL-A Antigens on Cultured Human Fibroblasts Infected with Mycoplasmas. Chaim Brautbar, Eric J. Stanbridge, Michele A. Pellegrino, Soldano Ferrone, Ralph A. Reisfeld, Rose Payne, and Leonard Hayflick . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1783

The Immune Response of Inbred Strains of Mice to a "High-Conjugation" and a "Low-Conjugation" 2,4 Dinitrophenyl-Glutamic AcidS'-LysineSS-Alanine 5 (DNP/GLA 5) Copoly, mer. Carol M. Warner, Paul H. Maurer, Carmen F. Merryman, and Mary Jo F. Schmerr . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1880

The Immune Response of Tetraparental Mice to Two Synthetic Amino Acid Polymers: "High-Conjuga- t ion" 2,4 Dinitrophenyl-Glutamic Acid57-Lysine38-Alanine 5 (DNP-GLA 5) and Glutamic Acid 6°- Alanine s° Tyrosine ~° (GATe°). Carol M. Warner, Maryann Fitzmaurice, Paul M. Maurer, Carmen F. Merryman, and Mary Jo F. Sehmerr . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1887

A Comparison of the Cytolytic Action of Guinea Pig and Rabbit Complement on Sensitized Nucleated Mouse Cells. Robert A. P. Koene and Ian F. C. McKenzie . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1894

IMMUNOPATHOLOGY

Immune Complex Glomerulonephritis in One-Year-Old C57BL/6 Mice Induced by Endogenous Murine Leukemia Virus and Erythrocyte Antigens. David D. Porter, Helen G. Porter, and Nancy A. Cox .. 1626

xiv C O N T E N T S [VOL. 111

M a c r o p h a g e - M e d i a t e d R e s i s t a n c e t o I n t r a c e l l u l a r I n f e c t i o n I n d u c e d b y P o l y - L - G l u t a m i c A c i d . S t a n t o n

G . A x l i n e , J o y W . M e n d e n h a l l , a n d J a c k S. R e m i n g t o n . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1634

Q u a n t i t a t i v e P h a g o c y t o s i s b y N e u t r o p h i l s . I. A N e w M e t h o d w i t h I m m u n e C o m p l e x e s . P . A . W a r d a n d

N . J . Z v a i f l e r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1771

Q u a n t i t a t i v e P h a g o c y t o s i s b y N e u t m p h i l s . II . R e l e a s e o f t h e C 5 C l e a v i n g E n z y m e a n d I n h i b i t i o n o f

P h a g o c y t o s i s b y R h e u m a t o i d F a c t o r . P . A . W a r d a n d N. J . Z v a i f i e r . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1777

T U M O R I M M U N O L O G Y

Q u a n t i t a t i o n o f M o u s e M a m m a r y T u m o r V i r u s ( M T V ) V i r i o n s b y R e d i o i m m u n o a s s a y . R o b e r t D .

C a r d i f f . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1722

E v i d e n c e fo r S e v e r a l Cel l P o p u l a t i o n s A c t i v e in A n t i b o d y D e p e n d e n t C e l l u l a r C y t o t o x i c i t y . J a c o b

Z i g h e l b o i m , B e n j a m i n B o n a v i d a , a n d J o h n L. F a h e y . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1737

I n t r o d u c t i o n o f C e l l u l a r I m m u n i t y t o a C h e m i c a l l y A l t e r e d T u m o r A n t i g e n . H s i a - F u C h a o , S t e p h e n C .

Peiper, Richard D. Aach, and Charles W. Parker ................................................. 1800

Differential Effect of Neuraminidase on the Immunogenicity of Viral Associated and Private Antigens of Mammary Carcinomas. Richard L. Simmons and Angelyn Rios ................................. 1820

Immunochemical Studies of the Intramolecular Heterogeneity of the Carcinoembryonic Antigen (CEA) of the Human Digestive System. J.M. Gold, C. Banjo, S. O. Freedman, and P. Gold ................ 1872


Human Antibody to Dengue Soluble Complement-Fixing (SCF) Antigens. William A. Falkler, Jr., A r w i n d R. D i w a n , a n d S c o t t B. H a l s t e a d . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1804

C O M M U N I C A T I O N S

I m m u n o g l o b u l i n o n t h e S u r f a c e o f H u m a n L y m p h o c y t e s . T h e D e t e c t i o n o f K a p p a C h a i n V R e g i o n D e t e r - m i n a n t s o n B Ce l l s a n d N o t o n T Cel l s . H o w a r d M . G r e y , S o n i a C o l o n , A l a n S o l o m o n , a n d C a r l a L.

M c L a u g h l i n . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1923

C h a r a c t e r i z a t i o n o f a S e c o n d N o r m a l A n t i g e n t h a t C r o s s - R e a c t s w i t h C E A . P . B u r t i n , G . C h a v a n e l , a n d

H . H i r s c h - M a r i e . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1926

Statement of Ownership, Management and Circulation required by the Act of October 23, 1962: Section 4369, Title 39, United States Code. 1. Date of Filing: October 1, 1973. 2. Title of Publication: Journal of Immunology. 3. Frequency of Issue: Monthly. 4. Locat4~n of known O~ce o/ Publication: 428 E. Preston St.,

Baltimore, Md. 21202. Editor: Joseph D. Feldman, M.D., Scripps Clinic and Re-

search Foundation, La Jolla, Calif. 92037. 7. Owners: (If owned by a c~)rporation, its name and address

must be stated and also immediately thereunder the names ami addre~es of s~ockholders owning or holding 1 percent or more of total amount of stock. If not owned by a corporation, the names and addresses of the individual owners must be given. If owned by a partnership or other unincorporated firm, its name and address, as well as that of each individual, must be given.) Ameri- can Association of Immunologists, 9650 Rockville Pike, Bethesda, Md. 20014. No stockholders.

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9. Paragraphs 7 and 8 include, in cases where the stockholder or security holder appears upon the books of the company as trustee or in any other fiduciary relation, the name of the person or corporation for whom such trustee is acting, also the statements in the two paragraphs show the affiant's full knowledge and belief as to the circumstances and conditions under which

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Run) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6123 5928 B. Paid Circulation

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t Single issue nearest to filing date. I certify tha t the statements made b y me above are correct

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A text for thinking bacteriologists...

Basic Bacteriology

- ° ~ ~ ° ° ~ ~ " " Its Biological and "1o "a _ 0 ,.,,." Chemical Background

~'~"' 0 0 ~ O ° Fourth Edition

Carl Lamanna, Ph.D., Deputy and Scientific Advi- sor, Life Sciences Division, Office of Chief of Re- search and Development. Department of the Army; M. Frank Malette, Ph.D., Professor of Biochem- istry, The Pennsylvania State University; and Leonard N. Zimmerman, Ph.D., Professor of Mi- crobiology, The Pennsylvania State University

For many years, this book has been recognized as a fundamental source of information. Every aspect of the study of bacteria is here, with emphasis on ideas and principles of physics, chemistry, and biology as used by the bacteriologist. New thoughts are evident in discussions of taxonomy, structure, genetics, and metabolism; chapters on energy transfer and biosynthesis are expanded accounts from previous editions. Nearh" 100 pages and 100 illustrations have been added. A precise treatment of bacteria as unique, viable subjects for study. 1973 / 1150 pages / 250 figures / $22.50

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