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CURRICULUM VITAE

Jitendra Singh Rathore, Ph.D (Fulbright Fellow) Assistant Professor School of Biotechnology Gautam Buddha University

Yamuna Expressway

Greater Noida

Uttar Pradesh

India- 201312

Email: jitendra@gbu.ac.in, jiteniitr@gmail.com,

http://www.cies.org/grantee/jitendra-singh-rathore Email:jitendra.rathore@fulbrightmail.org EDUCATION/PROFESSIONAL QUALIFICATION:

PhD (Biotechnology) from School of Biotechnology, Jawaharlal Nehru

University (JNU) New Delhi, and International Centre for Genetic Engineering

and Biotechnology (ICGEB) New Delhi, India.

M.Sc (Biotechnology) from Indian Institute of Technology (IIT),

Roorkee, Uttarakhand, India. (Ist Class)

Post Graduate diploma in Molecular Biochemical Technology

(Recombinant DNA Technology, Essential lab Techniques and Immunology)

from Sri Venkateshwara college, University of Delhi, India. (Ist Class)

B.Sc in Life Sciences (Zoology, Botany and Chemistry) from University

of Delhi, India. (Ist Class)

SUMMARY OF TEACHING/RESEARCH EXPERIENCE:

Sep 2014-Till date: Assistant Professor, School of Biotechnology,

Gautam Buddha University, (GBU) Greater Noida, U.P, India.

Sep 2013-Aug 2014: Assistant Professor of Microbiology on Visiting

Track Perelman School of Medicine, University of Pennsylvania (UPENN),

Philadelphia, USA.

Sep 2013-Aug 2014: Fulbright-Nehru Postdoctoral Fellow, Perelman

School of Medicine, University of Pennsylvania (UPENN), Philadelphia, USA.

July, 2010- Aug 2013: Assistant Professor, School of Biotechnology,

Gautam Buddha University, (GBU) Greater Noida, U.P, India.

Sep, 2009-June, 2010: Faculty Associate, School of Biotechnology,

Gautam Buddha University, (GBU) Greater Noida, U.P, India.

July 2004-Aug, 2009: Research Scholar, International Centre for

Genetic Engineering and Biotechnology, (ICGEB) New Delhi, India.

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Nov, 2002-July, 2004: Junior Research Fellowship, School of

Biotechnology, Jawaharlal Nehru University (JNU) New Delhi, India.

TEACHING EXPERIENCE (8 Year+ : Subjects taught at M.Tech/M.Sc

Biotechnology level)

1. Vaccine Design and Development.

2. Immunotechnology. 3. Introductory Bioinformatics. 4. Genetic Engineering and its Application.

5. Microbiology. 6. Industrial Biotechnology.

AWARDS AND ACHIEVEMENTS: 1. Fulbright-Nehru Postdoctoral Research Fellowship 2013-14 from United States-India Education Foundation (USIEF) USA. 2. Young Scientist Project Awarded under Fast Track Scheme from Department of Science and Technology (DST), Government of India, 2011. 3. Selected from Gautam Buddha University as a Teacher to attend 3rd Science conclave (Interaction with Nobel laureates and eminent scientist (An MHRD and DST initiative) 8th Dec-14th Dec, 2010 at IIIT Allahabad. 4. Pre doctoral travel award from American Society of Cell Biology (ASCB) USA 2007. 5. Foreign Travel Grant from Council of Scientific & Industrial Research (CSIR) for ASCB conference, 2007. 6. Foreign Travel Grant from Jawaharlal Nehru University (JNU) corpus fund. 2007. 7. Senior Research Fellowship (SRF) offered by Council of Scientific & Industrial Research, (CSIR) Govt. of India (Oct, 2005-Oct, 2008).

8. Junior Research Fellowship (JRF) offered by Council of Scientific & Industrial Research (CSIR)Govt. of India (Oct, 2003-Oct, 2005). 9. Qualified National Eligibilty Test (NET) for Lectrureship conducted by Council of Scientific & Industrial Research (CSIR) Govt. of India, 2002 10. Qualified National Level GATE examination in 2002. 11. Studentship for Master offered by Department of Biotechnology, (DBT) Govt. of India (2000-2002)

EDITOR/REVIEWER IN SCIENTIFIC JOURNALS:

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1. Reviewer SERB-DST Extramural Individual Centric Research Projects.

2. Ad-hoc reviewer for "Clinical Immunology" Journal ELSEVIER publication.

3. Ad-hoc reviewer for "Innate Immunity" Journal SAGE publication

4. Editor “Immunology and Infectious Disease” Journal from Horizon

Research publication corporation, USA.

5. Editor "Mathews Journal of Immunology & Allergy". Mathews International

Publishers USA.

6. Editor "Journal of Drug Metabolism & Toxicology": Open access USA

7. Editor "EC Pulmonology and Respiratory Medicine" Journal from E-

Cronicon publication London, UK.

8. Editor “Journal of Life Sciences Research” International Journal from

Asian online Journal.

9. Editor “Research and Review: A Journal for Bioinformatics” Journal from

STM Journal.

10. Editor "International Journal of Research in Science & Technology" IRA

Publication.

11. Editor "International Journal of Inventions in Engineering & Science

Technology" IRA Publication.

12. Editor "International Journal of Universal Science & Engineering" IRA

Publication.

13. Editor "Multidisciplinary International Journal" IRA Publication

M.TECH DISSERTATION/Ph.D SUPERVISION:

1. M.Tech Dissertation supervised : Twenty (20) 2. Ph.D: Two (02) ongoing

RESEARCH INTEREST:

Bacterial Persistence & Host-Pathogen Interaction (Pulmonary

Infection)

1. Bacterial Persistence: Toxin-antitoxin (TA) systems are small genetic

elements found on plasmids or chromosomes of countless bacteria, archaea, and possibly also unicellular fungi. Under normal growth conditions, the activity of the toxin protein or its translation is counteracted by an antitoxin protein or non coding RNA. Five types of TA systems have been proposed that differ markedly in their genetic architectures and modes of activity control. Subtle regulatory properties, frequently responsive to environmental cues, impact the behaviour of TA systems. Typically, stress conditions result in the degradation or depletion of the antitoxin. Unleashed toxin proteins impede or alter cellular processes including translation, DNA replication, or ATP or cell wall synthesis. TA toxin activity can then result in cell death or in the formation of drug-tolerant persister cells. The versatile properties of TA systems have also been exploited in biotechnology and may aid in combating infectious diseases.

2. Bacterial-Host Interaction (Pulmonary Infection Th17 response) To perform its primary function of gas exchange, the mucosal surface of the lungs

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is continuously exposed to the environment. Given the considerable infection risk to the host, complex mechanism exists to prevent the development of bacterial infection. The immune system in the lungs consists of both innate and adaptive components, and it is clear these two systems are highly interdependent for optimal host defense. With the recent discovery of a distinct subset of Th cells called Th17 cells, in addition to the previously well characterized Th1- and Th2-cell subsets, came many new breakthroughs in the realm of innate and adaptive immunity. Th17 cells have been shown to differentiate from naïve CD4+ cells in the presence of IL-6 and TGF-β in mice, or IL-6 and IL-1 in humans, when stimulated with appropriate antigen via activation of the transcription factor STAT3. As CD4+ cells commit to the effector Th17 phenotype, the hallmark cytokines IL-17A, IL-17F, IL-21, and IL-22 are expressed via STAT-3-dependent activation of the critical transcription factor retinoid-related orphan receptor γt. Th17 cells have

been found to play a vital role in host defense against numerous pathogens. However, while Th17 cells are paramount in the adaptive phases of host defense, several other cell types are able to generate these cytokines in the earlier phases of immune response and bridge the gap between innate and adaptive immunity in the lung. These cell types include γδ T cells, natural killer (NK) cells, NKT cells, and certain innate lymphoid cells. New vaccine approaches are needed against Streptococcus pneumoniae, Haemophilus influenzae, Bacillus anthracis and Mycobacterium tuberculosis, which are the keys pathogenic bacteria responsible for serious pulmonary infections in developing countries like India. Although Th17 cells can protect against gram-negative pathogens at mucosal surfaces, including the lung, the bacterial proteins recognized by Th17 cells are largely unknown and could be potential new vaccine candidates. I am interested in the identification of Th17-stimulating antigens

from Streptococcus pneumoniae, Haemophilus influenzae, Bacillus anthracis and Mycobacterium tuberculosis, using ORFeomics approach, which can be used as serotype-independent novel vaccine candidates in future. Technical expertise (key word): Genetic Engineering, Microbiology, Immunology, Bioinformatics, and Proteomics.

RESEARCH GRANTS APPROVED: 1. Transcriptional and Functional Characterization of Xenorhabdus

nematophila toxin-antitoxin (TA) modules. Department of Science and Technology (DST), Govt of India. Duration Three years (2012-2015) Amount 25 lakh. 2. Transcriptional, functional and persister cell formation studies of novel identified yefM-yoeB, ccdAB and hipAB toxin-antitoxin modules from Xenorhabdus nematophila. Council of Scientific and Industrial Research (CSIR)Three years (2016-2019) Amount 30 lakh

PUBLICATIONS:

INTERNATIONAL

1. Yadav M and Rathore JS (2018) TAome analysis of type-II toxin-antitoxin system from Xenorhabdus nematophila. Computational Biol and Chem Comput Biol Chem. 2018 Oct;76:293-301

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2. Gautam LK, Yadav M, and Rathore JS. (2017) Functional annotation of novel toxin-antitoxin system Xn-RelT of Xenorhabdus nematophila; a combined in silico and in vitro approach. J Mol Model 23 (6): 189 3. Pandey DK, Kumar A, Rathore JS, Singh N, Chaudhary B (2017) Recombinant overexpression of dihydroneopterin aldolase catalyst potentially regulates folate-biofortification. J Basic Microbiol. 2017 Apr 18. 57(6):517-524 4. Rathore JS and Yan Wang (2016) Protective role of Th17 cells in pulmonary infection. Review. Vaccine 34(13):1504-1514

5. GautamLK, YennamalliRM, Rathore JS (2016) Implication on the function of novel Xn-relE toxin structure of Xenorhabdus nematophila using Homology modeling. Current Bioinformatics 12(6): 535 - 542

6. Rathore JS and GautamLK. (2014) Expression, purification and functional analysis of novel relE operon from X. nematophila. The Scientific World Journal

Volume 2014 (2014) 428159

7. Rathore JS. (2013) Expression, purification and functional characterization of atypical xenocin, its immunity protein and their domains from Xenorhabdus nematophila. International Journal of Bacteriology Volume 2013 (2013), Article ID 746862

8. Rathore JS. (2013) Function inferences from a molecular structural model of YoeBXn toxin from Xenorhabdus nematophila. American Journal of Bioinformatics Research. 3 (2): 25-29

9. Rathore JS*, Singh MP, and Gautam P. (2013) Insilico analysis of novel hipAB, ccdBA, and yoeB-yefM Toxin-Antitoxin homolog’s from the Genome of Xenorhabdus nematophila. American Journal of Bioinformatics Research. 3 (2):11-20 (*Corresponding author)

10. Singh, J. (2013) Role of D535 and H538 in endogenous toxicity of xenocin

from Xenorhabdus nematophila. FEMES Microbiology letters 338: p 147-154. 11. Sameer Giri and Singh, J*(2013) New Face in the Row of Human therapeutics: Bacteriocins. Journal of Microbiology Research, 3 (2) p71-78 (*Corresponding author)

12. Singh J. (2012) Structural and Functional Interferences from a Molecular Structural Model of Xenocin Toxin from Xenorhabdus nematophila. American

Journal of Bioinformatics Research. 2 (4):p55-60

13. Singh, J*.,Chaudhary, R, K., Gautam, P. (2012) Insilico analysis of novel relB, relE and mazF toxin-antitoxin homolog’s from the genome of Xenorhabdus nematophila. 2(3): p21-32. American Journal of Bioinformatics Research (*Corresponding author)

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14. Singh, J*. andGhosh C. (2012) Ribosomal Encoded Bacteriocins: Their functional insight and applications. Journal of Microbiology Research 2(2): p19-25 (*Corresponding author)

15. Singh, J. and Banerjee, N. (2008) Transcriptional analysis and functional characterization of a gene pair encoding iron regulated xenocin and immunity proteins of Xenorhabdus nematophila. Journal of Bacteriology, 190: p3377-85

16. Banerjee, J.,* Singh, J*, (2006) Mohan Chandra Joshi, ShubhenduGhosh, and Nirupama Banerjee. The Cytotoxic Fimbrial Structural Subunit of Xenorhabdus nematophila Is a Pore-Forming Toxin. Journal of

Bacteriology. 188(22): 7957-7962. (*equal contribution).

17. Singh, J., Joshi, M.C., and Bhatnagar, R. (2004). Cloning and expression of glutamine synthetase gene from mycobacterium in E. coli. Biochemical and

Biophysical Research Communication. 317: 634-638

NATIONAL

1. Chaitali Ghosh, Harish Kumari and Jitendra Singh Rathore, "Prevalance and risk of listeriosis by ready to eat products", International Journal of Scientific Research Letters Vol 1, Issue 1, Dec 2017 2. Sharma P, Ghosh C, Rathore JS. Anthocyanin: Structure, Synthesis and its Beneficial Role in Health. Research & Reviews: Journal of Food Science and Technology. 2016; 5(1): 45–55p.

BOOK CHAPTER

1. Jitendra Singh Rathore and Chaitali Ghosh "Pathogen-Associated Molecular

Patterns and Their Perception in Plants", chapter published in the book titled

"Molecular Aspects of Plant-Pathogen Interaction", published by Springer Nature,

ISBN 978-981-10-7370-0 ISBN 978-981-10-7371-7 (eBook).

MODEL SUBMITTED IN MODEL ARCHIVE

1. http://www.modelarchive.org/doi/10.5452/ma-azcde (2016)

2. http://www.modelarchive.org/doi/10.5452/ma-abgt9 (2016)

SEQUENCES SUBMITTED IN NCBI DATBASE:

1. Singh, J. and Banerjee, N. AAZ06276.( 2005). Nucleotide sequence

submitted in NCBIdata base. Direct submission. Putative colicin like protein

[Xenorhabdus nematophila] gi|70671870|gb|AAZ06276.1|[70671870]

2. Singh, J., and Banerjee, N. AAZ06277. (2005). Nucleotide sequence

submitted in NCBIdata base. Direct submission. Putative hemagglutinin/hemolysin

[Xenorhabdus nematophila gi|120433596|gb|AAZ06 277.1|[120433596]

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3. Singh, J., and Banerjee, N. AAZ06278. (2005). Nucleotide sequence

submitted in NCBIdata base. Direct submission. Putative cytoplasmic protein

[Xenorhabdus nematophila] gi|70671872|gb|AAZ06278.1|[70671872]

4. Singh, J., Shau, A. K., and Banerjee, N. ABC24675. (2005) Nucleotide

sequence submitted in NCBIdata base. Direct

submission.Glycerophosphoryldiesterphosphodiesteras precursor [Xenorhabdus

nematophila]

ABSTRACTS ACCEPTED IN INTERNATIONAL/NATIONAL CONFERENCES

1. Jitendra Singh Rathore (2017). Study of Th17 antigens from Streptococcus pneumoniae at International Vaccine Conference at International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, 27-29 Nov 2017 2. Mohit Yadav and Jitendra Singh Rathore. (2017). Structural and functional inferences of a novel hipBAXn toxin-antitoxin module from Xenorhabdus nematophila. Abstract accepted in INSCR International conference (IIC-2017) University of Delhi 26-28 September 2017. 3. Mohit Yadav and Jitendra Singh Rathore. (2017). TAome analysis of type-II toxin-antitoxin system from entomopathogenic bacteria Xenorhabdus nematophila. Abstract accepted for Poster presentation at 58th International Annual Conference of The Association of Microbiologists of India (AMI- 2017) at Babasaheb Bhimrao Ambedkar University, Lucknow, Uttar Pradesh. 4. Mohit Yadav and Jitendra Singh Rathore. (2017). In-silico interactomic analysis of Xn-HipAB TA module: structural and gene ontological inferences from Xenorhabdus nematophila. National Conference on Development and Advancement in conservation, propogation and sustainable utilization of medicinal plants. 20-21 January 2017. School of Biotechnology, Gautam Buddha University, Greater Noida. Sponsored by National Medicinal Plants Board, Ministry of AYUSH, Govt of India. (BEST POSTER AWARD) 5. Anjali Gautam and Jitendra Singh Rathore (2017).. Homology modelling based structural assessment and functional derivation of novel YoEB toxin from

Xenorhabdus nematophila and its interaction with YefM antitoxin. National Conference on Development and Advancement in conservation, propogation and sustainable utilization of medicinal plants. 20-21 January 2017. School of Biotechnology, Gautam Buddha University, Greater Noida. Sponsored by National Medicinal Plants Board, Ministry of AYUSH, Govt of India. (BEST POSTER AWARD) 6. Deeksha Duggal, Mohit Yadav and Jitendra Singh Rathore. (2017). Identification and characterization of putative Xn-FIC- PHD Toxin-antitoxin module from Xenorhabdus nematophila. National Conference on Development and Advancement in conservation, propogation and sustainable utilization of medicinal plants. 20-21 January 2017. School of Biotechnology, Gautam Buddha University, Greater Noida. Sponsored by National Medicinal Plants Board, Ministry of AYUSH, Govt of India.

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7. Gaurav kant, Mohit Yadav and Jitendra Singh Rathore. (2017). Gene ontology study of novel putative Xn-GNAT- RHH toxin antitoxin module from Xenorhabdus nematophila. National Conference on Development and Advancement in conservation, propogation and sustainable utilization of medicinal plants. 20-21 January 2017. School of Biotechnology, Gautam Buddha University, Greater Noida. Sponsored by National Medicinal Plants Board, Ministry of AYUSH, Govt of India. 8. Kirti Vashisth, Mohit Yadav and Jitendra Singh Rathore. (2017). In-silico structural and functional analysis of novel Xn-MosAT module from Xenorhabdus nematophila. National Conference on Development and Advancement in conservation, propogation and sustainable utilization of medicinal plants. 20-21 January 2017. School of Biotechnology, Gautam Buddha University, Greater Noida. Sponsored by National Medicinal Plants Board, Ministry of AYUSH, Govt of India.

9. Anamika chaudhary, Mohit yadav and Jitendra Singh Rathore. (2017). 3D modeling and functional inferences of a novel Xn-PIN- PHD like Toxin-antitoxin module from Xenorhabdus nematophila. National Conference on Development and Advancement in conservation, propogation and sustainable utilization of medicinal plants. 20-21 January 2017. School of Biotechnology, Gautam Buddha University, Greater Noida. Sponsored by National Medicinal Plants Board, Ministry of AYUSH, Govt of India. 10. Shubhrankita Pandey, Mohit Yadav and Jitendra Singh Rathore. (2017). 3D structure and function prediction of a novel Xn-GNAT- RHH Toxin-antitoxin system from Xenorhabdus nematophila. National Conference on Development and Advancement in conservation, propogation and sustainable utilization of medicinal plants. 20-21 January 2017. School of Biotechnology, Gautam Buddha University, Greater Noida. Sponsored by National Medicinal Plants Board, Ministry of AYUSH, Govt of India. 11. Rupal Chaudhary, Mohit Yadav and Jitendra Singh Rathore. (2017). In-silico characterization of putative Xn-RelE- RHH Toxin-Antitoxin Module from Xenorhabdus nematophila. National Conference on Development and Advancement in conservation, propogation and sustainable utilization of medicinal plants. 20-21 January 2017. School of Biotechnology, Gautam Buddha University, Greater Noida. Sponsored by National Medicinal Plants Board, Ministry of AYUSH, Govt of India. 12. Mohit Yadav1 , Chaitali Ghosh2 , Jitendra Singh Rathore1. Functional and transcriptional analysis of novel Xn-relB and Xn-relE toxin-antitoxin modules from Xenorhabdus nematophila.(1 School of Biotechnology, Gautam Buddha University, Greater Noida, Uttar Pradesh, India, 2Department of Zoology, Gargi College, University of Delhi, New Delhi, India.) Poster presented in 56th Annual Conference of Association of Microbiologists of India (AMI) 7th -10th December, Jawaharlal Nehru University, JNU, New Delhi, India2015 13. Mohit Yadav1 , Jitendra Singh Rathore. Evaluation of Xn-relB abd Xn-relE promoter(s) from Xenorhabdus nematophila by LacZ reporter construct .(1 School of Biotechnology, Gautam Buddha University, Greater Noida, Uttar Pradesh, India) .) Poster presented in Industrial-Academia Meet in Gautam Buddha University, Greater Noida, Uttar Pradesh, 2015 (Won First Prize) 14. Singh, J. 1,2 and Banerjee, N. 2 (1 School of Biotechnology, Gautam Buddha University, Greater Noida, Uttar Pradesh, India.2 International Centre for Genetic Engineering and Biotechnology, New Delhi, India.) Role of D535 and H538 in

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endogenous toxicity of xenocin from Xenorhabdus nematophila. Poster presented at 111th General meeting American society of for microbiology (ASM), New Orleans, Louisiana May 21-24, 2011. 15. Banerjee, N.,1 Singh, J., 1 Singh, P., 1 Forst, S., 2 and Park D. 2 (1Insect Resistance Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India, 2.Department of Biological Sciences, University of Wisconsin, Milwaukee, WI) “Xenocin Export in Xenorhabdus nematophila”. Abstract accepted for 49th annual meeting of American Society for Cell Biology (ASCB) at San Diego, CA December 5-9, 2009. 16. Singh, J., Banerjee, N., and Bhatnagar R. "Secretion studies of xenocin operon from Xenorhabdus nematophila” Abstract accepted for 109th General

meeting of AmericanSociety for Microbiology (ASM) at Philadelphia, Pennsylvania, USA May 2009. 17. Singh, J., Banerjee, N., and Bhatnagar R." Transcriptional analysis and functional characterization of a gene pair encoding iron regulated xenocin and immunity proteins of Xenorhabdus nematophila” poster presented at Annual Conference of American Society for CellBiology (ASCB) at Washington DC, USA December 2007. PROFESSIONAL MEMBERSHIPS: 1. Member of American Society for Microbiology (ASM), USA, Annual. 2. Member of American Society for Cell Biology (ASCB), USA, Annual.

3. Life Time member of Association of Microbiologist of India (AMI), India.

CONFERENCES ATTENDED:

1. International Vaccine Conference at International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, 27-29 Nov 2017 2. 10th Orientation program from UGC-HRDC Jawaharlal Nehru University (JNU) New Delhi 13 Feb 2017-10 March 2017 (26 Days) 3. Participated in INSCR International conference (IIC-2017) at University of Delhi, 26-28 September 2017.

4. Under Graduate Science teacher workshop "Developing scientific temper in student: Tools and Techniques" at Ramjas college 31 Jan 2017 , University of Delhi. Funded by Department of Biotechnology and MHRD Govt of India. 5. National Conference on "Development and Advancement in conservation, propogation and sustainable utilization of medicinal plants". 20-21 January 2017. School of Biotechnology, Gautam Buddha University, Greater Noida Sponsored by National Medicinal Plants Board, Ministry of AYUSH, Govt of India. 6. Chair session for "Intellectual Property Awareness Program 2015-16", Intellectual Property & Innovation Management in University on 10th March 2016, Gautam Buddha University.

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7. 56th Annual Conference of Association of Microbiologists of India (AMI) 7th -10th December 2015, Jawaharlal Nehru University, JNU, New Delhi, India. 8. PENNFLOW 2014 “Flowing outside the box” by PathBioResource Flow Cytometry, Perelamn School of Medicine, University of Pennsylvania, USA.

9. Lecture workshop on "Trans-disciplinary area of Research and Teaching by Shanty Swaroop Bhatnagar Awardee" Feb 01-02, 2013 Organized by Deen Dayal Upadhyaya College, University of Delhi and IEEE EDS Delhi Chapter, New Delhi.

10. Singh, J. 1,2 and Banerjee, N. 2 (1 School of Biotechnology, Gautam Buddha University, Greater Noida, Uttar Pradesh, India.2 International Centre for Genetic

Engineering and Biotechnology, New Delhi, India.) Role of D535 and H538 in endogenous toxicity of xenocin from Xenorhabdus nematophila. Poster presented at 111thGeneral meeting American society of for microbiology (ASM), New Orleans, Louisiana May 21-24, 2011.

11. Third Science conclave: "Interaction with Nobel laureates and eminent scientists" (An MHRD and DST initiative) 8th Dec-14th Dec, 2010 at IIIT Allahabad.

12. “The cytotoxicity fimbrial structural subunit of Xenorhabdus nematophila is a pore forming toxin”. Poster presented in Ist Annual Symposium of School of Biotechnology, Jawaharlal Nehru University, New Delhi, 2009.

13. Attended 2nd International conference on "Trends in cellular and Molecular Biology", organized by School of Life Sciences, Jawaharlal Nehru University, New Delhi, 2008.

14. "Transcriptional analysis and functional characterization of a gene pair encoding iron regulated xenocin and immunity proteins of Xenorhabdus nematophila”. Poster presented at Annual Conference of American Society for Cell Biology at Washington DC, USA December 2007.

15. “MEDIATORS OF PATHOGENECITY OF Xenorhabdus nematophila”. Poster presented at BIOTECH 2005 Concept of Commercialization, organized by School of Biotechnology, Jawaharlal Nehru University, New Delhi.

16. Attended workshop on “Patent And Intellectual Property Rights Awareness” sponsored by Technology Information Forecasting & Assessment Council (TIFAC), New Delhi. “BIOPOP" 2002 conducted by Department of Biotechnology, IIT, Roorkee

SEMINARS ATTENDED:

1. “Protective immunity against invasive Streptococcus pneumoniae infections by tissue-specific memory Th17 cells” by Yan Wang, Ph.D.University of Pennsylvania, USA, 2013. 2. “Influences of the cytokine interleukin-22 on mucosal barriers” by Lauren Zenewicz, Ph.D., University of Oklahoma Health Sciences Center, USA, 2013.

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3. “Host and bacterial factors influencing pneumococcal transmission” by Aimee Richard, Ph.D University of Pennsylvania, USA, 2013. 4. “Yersinia pestis and early events in bubonic plague’. Virginia Miller, Ph.D. University of North Carolina, USA, 2013. 5. “Modeling the efficacy of natural and vaccine-induced CD8+ T cell responses to HIV in humanized mice”. Todd Allen, Ph.D.Ragon Institute of MGH, MIT and Harvard,USA, 2013. 6. “Surviving intoxication: Host regulation of pore forming toxins” by Mary O'Riordan, Ph.D.University of Michigan Medical School, USA, 2013.

7. “Structural and functional studies of type IV pili” Katrina Forest, Ph.D., University of Wisconsin-Madison, USA, 2014. 8. “Newly recognized levels of Vibrio cholera virulence gene regulation” Ronald Taylor, PhD, Dartmouth University, USA, 2014. 9. “Filamentation of E. coli by hyperactivation of PhoQP two-component system” Srujana Samhita Yadavalli , Ph.D. University of Pennsylvania, USA, 2014. 10. "Cytosolic access of Streptococcus pneumoniae cell wall components in macrophages is dependent on pneumolysin”Jamie Lemon Ph.D. University of Pennsylvania, USA, 2014.

11. “Virulence Functions of Coxiella burnetii's Type IV Secretion System” Liam Bradley Ph.D.University of Pennsylvania, USA, 2014. 12. “Non-typeable Haemophilus influenzae immune evasion; resistance to complement- and neutrophil-mediated killing” Jeroen Langereis, Ph.D.University of Pennsylvania, USA, 2014.

RESEARCH EXPERIENCE:

1. Gautam Buddha University (GBU), School of Biotechnology, Greater Noida as Assistant Professor and Principal Investigator CSIR and DST project (July 2012-Aug 2019). 2. Perelman School of Medicine, University of Pennsylvania (UPENN), Philadelphia, USA (Sep, 2013-Aug 2014). Fulbright-Nehru Postdoctoral Research sponsored by United States-India Education Foundation commission. 3. Gautam Buddha University (GBU), School of Biotechnology, Greater Noida as Assistant Professor (Sep2009-Sep2013). 4. International Centre for Genetic Engineering and Biotechnology, (ICGEB), New Delhi. (July, 2004-Aug, 2009) Ph.D Scholar work entiled “Characterization of 4.3kb fragment from the genome of Xenorhabdus nematophila containing xenocin and its immunity gene(s)” in Insect Resistance Group.

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5. School of Biotechnology, Jawaharlal Nehru University,(JNU) New Delhi (Nov, 2002 - July,2004) Junior Research Fellow work entiled “Cloning and expression of glutamine synthetase gene from mycobacterium in E.coli” under supervison of Prof. Rakesh Bhatnagar. 6. Department of Biotechnology, All India Institute of Medical Science (AIIMS).(May 2001 -July 2001) “Cloning and expression of a gene from Plasmodium vivax” under supervision of Head of the Department, Prof. Y. D. Sharma (Summer Training). SPECIALIZED TRAINING ATTAINED:

1. Species-Specific Hands-on Training -- Mouse – ULAR, University of

Pennsylvania, USA

2. Mouse Training Pre-requisite – ULAR, University of Pennsylvania, USA

3. Occupational Health and Safety Animal Users Orientation – ULAR,

University of Pennsylvania, USA

4. Recombinant DNA Training – EHRS ,University of Pennsylvania, USA

5. Regulations and the IACUC: Module 6: Conclusions and Final Exam-

IACUC, University of Pennsylvania, USA

6. Regulations and the IACUC: Module 5-Working Safely with Animals-

IACUC, University of Pennsylvania, USA

7. Regulations and the IACUC: Module 4-Guidelines for Specific Research

Procedures- IACUC, University of Pennsylvania, USA

8. Regulations and the IACUC: Module 3-The Protocol Basics-IACUC,

University of Pennsylvania, USA

9. Regulations and the IACUC: Module 2-Federal Regulations and the

IACUC-IACUC, University of Pennsylvania, USA

10. Regulations and the IACUC: Module I-Introduction-IACUC, University of

Pennsylvania, USA

11. Introduction to Laboratory and Biological Safety at Penn-EHRS,

University of Pennsylvania, USA

12. Flow Cytometry and Cell Sorting, University of Pennsylvania, USA

EXPERIENCE IN RESEARCH LABORATORY: ANIMAL STUDIES: Mice Handling, Bacterial infection in Lung, Lung and Spleen isolation, Single cell preparation form Lung and Spleen, in vitro stimulation, CD4+ and CD8+ cell purification from Lung and Spleen, FACS. MOLECULAR BIOLOGY: Isolation of plasmid & Genomic DNA, Genomic Library preparation,Gene cloning, Polymerase Chain reaction (PCR), Agarose Gelelectrophoresis, Colony Hybridization, Northern and Southern Blotting, Western blotting, DNA Sequencing, Electroporation, total RNA isolation,RT-PCR, Primer Extension and Site directed mutagenesis (SDM). PROTEIN SCIENCE: Purification of membrane proteins, over expression of recombinantproteins, purification of recombinant proteins, refolding of inclusionbodies and protein binding & inhibition studies.

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BIOPHYSICS: Absorption spectroscopy, Spectropolarimetry (CD), Spectrofluorometry,Gel permeation & Ion exchange chromatography, FPLC, PAGE (native &SDS). MICROBIOLOGY: Isolation of Bacterial strains from soil and Insect gut, Growth Inhibitionassay, Cell culturing. IMMUNOLOGY: Immunization based studies on mouse animal model, Immunoblot analysis,Ouchterlony's double diffusion technique, ELISA, neutralization assay,agglutination, immunofluorescence, and generation of antiserum. TISSUE CULTURE: Maintenance of macrophage and various human cancerous cell lines andinsect cell lines, cytotoxicity assay (MTT).

TYPES OF INSTRUMENTS, SYSTEMS, SOFTWARES ETC INSTRUMENT USED: BD Canto, AKTA Explorer, PCR, Tycoon scanner for radio labeled gel,

hybridization chamber, scintillation counter, SDS PAGE, Western apparatus,

lypholyzer, Ultra centrifuge

SOFTER USED IN BIO-INFORMATICS: Expasy server, EBI server, CHIMERA software, RASMOL, Multiple alignments,

similarity searches, BLAST, Microbial BLAST version 2.2.6, FASTA, ClustalW

version 1.8, ClustalX 1.8, Multalin, primer designing, PROSITE, RDP-II Codon

Usage Database

ADMINISTRATIVE EXPERIENCE AT GBU: 1. Member of Institution Bio-safety committee (IBS), GBU. 2. Member of Ph.D committee School of Biotechnology, GBU. 3. Member of Seminar committee School of Biotechnology, GBU 4. Member of Chemicals and consumable committee School of Biotechnology, GBU Former 5. Member of Admission committee, GBU. 6. Former Member of Proctorial Board GBU. 7. Former Warden of Sant Kabir Das Boys Hostel GBU. 8. Former Member of Departmental Purchase Committee (DPC). 9. Former Member of Departmental Equipment Purchase Committee 10. Former Member of Departmental Research Project Committee. 11. Designed Ph. D programme for School of Biotechnology. 12. Former Member of Instrumental Committee of “ABHIBIYANJANA” 13. Member of Departmental Horticulture Committee.

PERSONAL DETAILS

Nationality: Indian Languages: English, Hindi, Pahari, Punjabi and Bengali Bonafide: Himachal Pradesh

Domicile: Delhi

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ACADEMIC REFEREES 1. Prof. Rakesh Bhatnagar (Ph.D Supervisor) Vice-Chancellor

Banaras Hindu University

Varanasi - 221005, U.P., India

Telephone: +91(542) 2368938 (Off); 2368339 (Resi)

Fax: +91(542)2369100 (Off); 2369951 (Resi)

Email: vc@bhu.ac.in Email: rakeshbhatnagar@mail.jnu.ac.in

2. Prof. Rajiv Bhat ( Former Dean) School of Biotechnology,

Jawaharlal Nehru University,

New Delhi 110067 India.

Phone: +91 11 26704086 (off) +91 11

26742098(Res) 5. Fax: +91 11 26717040, +91 11 26717586 Email: rajivbhat@mail.jnu.ac.in

3. Dr. Hao Shen, PhD Department of Microbiology Perelman School of Medicine University of Pennsylvania 303C Johnson Pavilion 3610 Hamilton Walk Philadelphia, PA 19104-6076 Email: hshen@mail.med.upenn.edu Phone: 215-573-5259