JASMONATE AND ETHYLENE SIGNALS IN PLANT DEFENCE2 jai1-3 jin1 coi1-1 ein3-3 jai1-1 jai1-2 jai1-4...
Transcript of JASMONATE AND ETHYLENE SIGNALS IN PLANT DEFENCE2 jai1-3 jin1 coi1-1 ein3-3 jai1-1 jai1-2 jai1-4...
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JASMONATE AND ETHYLENE SIGNALS IN PLANT DEFENCE
Roberto SolanoDpto. Genética Molecular de PlantasCentro Nacional de Biotecnología (CNB-CSIC)e-mail: [email protected]
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Hormonal signalling in plant defence against pathogens
Pathogens
ET JA
PRs(acidic)
SA
npr1
PDF, b-CHI(basic PRs)
Biotrophs Necrotrophs
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JR1JR2VSP
JAOGAs
C2 H4 Systemicsignal
JR1JR2VSP
WOUNDED LEAF SYSTEMIC LEAF
CKWR3
MECHANICALDAMAGE
ETR1EIN2EIN3
COI1
ET prevents JA-mediated gene expression in the wound response
Rojo et al., 1999
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ET mediates cell-death in response to O3
Rao et al., 2002
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JA prevents ET-mediated cell-death in response to O3
Overmyer et al., 2000
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JA prevents ET-induced hook curvature
Ellis and Turner, 2002
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Pathogens(necrotrophs)
ET JA
ERF1(other ERFs) ?
PRs
disease resistance
Lorenzo et al., 2003
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Botrytis cinerea induces ERF1 expression
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JA regulates ERF1 expression
ERF1 expression requiresET and JA pathways simultaneously
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ERF1 restores PR expression in coi1 and ein2
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ERF1 increases resistance to necrotrophic fungi
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Pathogens(necrotrophs)
ET JA
ERF1(other ERFs) ?
PRs
disease resistanceLorenzo et al., 2003
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Disection of Arabidopsis-Pythium interaction
+
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Pathogens(necrotrophs)
ET JA
ERF1(other ERFs) ?
PRs
disease resistance
Lorenzo et al., 2003
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Genetic disection of the JA signaling pathway
Described Mutantscoi1
Chromosome IIjin4/jar1
Chromosome Ijin1
WT ein3
50µMJA
WTcoi1
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JA insensitive mutants (jai)
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Map position of jai mutants
cM
25 jai4 (1 alleles)jai3
(2 alleles)50
75
100
125
150
I II III IV V
jai1(5 alleles)
jai2 (3 alleles)
coi1jar1
jai5 (7 alleles)
jin1
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jai4 is more susceptible to Botrytis
0
20
40
60
80
100
Col-0edm
1ein
3-3jai4
-1coi
1-1
0 1 2 3 4
Col-0
edm1
ein3-3
jai4-1
coi1-1
Disease rating%
Infe
cted
leav
es
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jai4 is insensitive to auxins
ein3-3 Col-0 jai4 coi1 axr1-12 aux1-7
50µM JA
0,3µM NAA
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Expression of auxin-regulated genes in jai4
C JA Ax C JA Ax C JA Ax C JA Ax C JA Ax C JA Axein3-3 jai4-1 Col-0 jin1 axr1-12edm1
IAA2
IAA4
JR1
JR3
VSP2
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jai4 interacts genetically with axr1
Col-0ein3-3 jai4-1 jai4-1;axr1 axr1 edm1;axr1 edm1
NAA
Col-0ein3-3 jai4-1 jai4-1;axr1 axr1 edm1;axr1 edm1 coi1
JA
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Positional cloning of jai4
Chromosome IV CIW6DET1
F25I24 F8L21F25E4
T5C23T22B4
SGT1b
TGG(Trp)
TAG(stop)
TRP CS SGS
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The SCF ubiquitin ligase model
Gray, 2002
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Sgt1a is insensitive to low JA concentrations
WS Col-0jai4-1 ein3-3 coi1 edm1rar1 sgt1a
50 µM JA
5 µM JA
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JA insensitive mutants (jai)
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Mutations in JIN1 increase resistance to necrotrophic fungi
Disease rating
0
20
40
60
80
100
Col-
0ja
i1-2
jai1
-3jin
1co
i1-1
ein3
-3ja
i1-1
jai1
-2ja
i1-4
jai1
-5
% in
fect
ed le
aves
0 1 2 3
Col- 0 ein3-3
P. cucumerina
Disease rating
0
20
40
60
80
100
Col-
0ja
i1-2
jai1
-3jin
1co
i1-1
ein3
-3ja
i1-1
jai1
-2ja
i1-4
jai1
-5
% in
fect
ed le
aves
0 1 2 3
Col- 0 ein3-3
B. cinerea
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Expression profiling of jin1 in response to JA
WT vs. jin1JA :
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Expression profiling of jin1 in response to JA
(WT vs. jin1) JAPathogen Defence
(PDF, CHI, Hevein)
Wounding(VSP, LOX, MBP)
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Differential regulation of JA-response genes by JIN1
jin1-2 jin1-1
Wounding-responsegenes
Pathogen-responsegenes
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Positional cloning of jin1
Chromosome I AIG1UFO
F27G20 T9G5F6N18
F9L11T9L6
AtMYC2
T16O9F10C21F5D14F3C3
bHLHAcidic
S588N∆ jin1
W103Zjai1-1
W194Zjai1-2
G228Ejai1-3
R239Zjai1-4
W266Zjai1-5
T- DNA3 SALK_061267
T- DNA2 SALK_040500
T- DNA1 SALK_039235
ZIP
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T-DNA insertions in AtMYC2 induce JA insensitivity
SALK_061267SALK_040500SALK_039235
jin1-2 (jin1-7) (jin1-8)WTT-DNA1 T-DNA2 T-DNA3
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jin1-1;35S:AtMYC2
jin1-
2
jin1-2;35S:AtMYC2
jin1-
1
JA
(5µM)
(10µM)
Col-0;35S:AtMYC2
Col-0
1 2 1 2 1 2
AtMYC2
AtMYC2 induces JA hypersensitivity
jin1-
2
jin1-
1
Col-0
1 2 1 2 1 2
rDNA
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JA (µM) 5 10AtMYC2 is a nuclear protein
Col- 0
jai1-2
jin1
35S:GFP 35S:AtMYC2:GFP 35S:AtMYC2∆:GFP
bHLH ZIP
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AtMYC2 expression is regulated by Wounding, JA and ABA
-1
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ET prevents JA-mediated gene expression in the wound response
JR1JR2VSP
JAOGAs
C2 H4 Systemicsignal
JR1JR2VSP
WOUNDED LEAF SYSTEMIC LEAF
CKWR3
MECHAN ICALDAMAGE
ETR1EIN2EIN3
COI1
Rojo et al., 1999
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ERF1 represses wound response downstream AtMYC2
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Pathogens(necrotrophs)
ET JA
ERF1
Pathogen-response genes
Wounding(mechanical/insect/hervibory)
AtMYC2
(ABA)?
COI1
Wound-response genes
SGT1b
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Aknowledgements
CNB (lab. 317)
Marta Godoy
Genomics facility CNB
Oscar LorenzoJA signalling
Irene López-VidrieroJosé M. ChicoCatalina del Moral
Bruce AdiePythium
Luis López
Genomics facility DIO (CNB)Gloria García-Casado
Julián PérezGenomics
Sylvia Gutiérrez
ConfocalCristina Merino
José J. Sánchez-Serrano
CNB (lab. 316)