Janne Kudsk Klitgaard, SDU · How does it work? –look at expression of all genes Fold change Gene...
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Can parts of the cannabis plant prevent multidrug resistance WHINN.dk #WHINN Janne Kudsk Klitgaard, SDU
Transcript of Janne Kudsk Klitgaard, SDU · How does it work? –look at expression of all genes Fold change Gene...
Can parts of the cannabis plant prevent multidrug resistance
WHINN.dk
#WHINN
Janne Kudsk Klitgaard, SDU
Antibiotic resistance in the future
Source: Antimicrobial Resistance: Tackling a crisis for the health and wealth of nations
The Review on Antimicrobial Resistance Chaired by Jim O’Neill, December 2014
Antibiotic resistance development
Compound tested MIC (µg/mL)
Cannabidiol (CBD) 4
Cannabigerol (CBG) 4
Cannabichromene (CBC) 4
Dicloxacillin 0.25
Vancomycin 2
Daptomycin 16
Bacitracin 64
Antimicrobial effect of cannabinoids against
Methicilin-resistant Staphylococcus aureus (MRSA)
MIC – Minimal inhibitory concentration – the lowest concentration that can inhibit growth
CBD as a helper compound in combination with
an antibiotic is a more efficient killer
MIC
BAC
(µg/mL)
MIC
BAC Combi
(µg/mL)
Staphylococcus
aureus (MRSA)
64 2-4
Staphylococcus
epidermidis (MRSE)
64 1
Enterococcus faecalis 128 8
Listeria
monocytogenes
512 32-64
Patent no. PCT/EP2018/066234
Bacitracin and/or daptomycin combined with cannabidiol for treatment of bacterial infections
How does it work? – ElectronmikroscopiUntreated
Cannabidiol
Bacitracin
Combination
How does it work? – effects on the membrane
Untr
eate
d
CCCP C
ontrol
CBD
0
1
2
3
4
5
6
7
Membrane Potential
***
Rati
o
(Red
/Gre
en
)
Membrane potential is the difference in electric potential between the interior and the exterior of a biological cell.
Membrane
function is
disturbed
How does it work? – look at expression of all genes
Fold change
Gene Function CBD BAC Combi
cwrA Function Unknown, Responds to Cell Wall Damage 6.5 29.9 256
hrtA Heme Efflux Pump 9.4 - -
farE Fatty Acid Efflux Pump 5.9 - -
vraD Bacitracin Transporter - 204.3 116.4
vraR Regulator of Cell Wall Stress Stimulon - - 10.8
pyrF Oritidine 5’-Phosphate Decarboxylase, RNA Synthesis 0.17 0.27 0.08
oppA Oligopeptide ABC Transporter 4.6 - 2.1
dapA Lysine Synthesis 3.8 - 3.9
SAUSA300_2453 ABC Transporter of Unknown Specificity - 0.13 0.04
Genes involved in
membrane and
cell wall function
are turned on/off
Membrane
function is
disturbed
Cell wall
seems
normal
Future work
3 year collaboration agreement with Canna Therapeutic ApS
▪ Find mechanism of action
▪ Toxicity evaluation
▪ Develop product for topical application
▪ Animal experiments
Thank you
Claes Søndergaard Wassmann
Founding and collaboration:
SDU Research & Innovation Organisation
Steen Plesner Pontoppidan, Canna Therapeutic ApS
Thank you for your attention ☺
