J. Dairy Sci. 101:4615–4637 ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance...

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J. Dairy Sci. 101:4615–4637 https://doi.org/10.3168/jds.2017-13736 © 2018, THE AUTHORS. Published by FASS Inc. and Elsevier Inc. on behalf of the American Dairy Science Association ®. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

Transcript of J. Dairy Sci. 101:4615–4637 ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance...

Page 1: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

J. Dairy Sci. 101:4615–4637https://doi.org/10.3168/jds.2017-13736© 2018, THE AUTHORS. Published by FASS Inc. and Elsevier Inc. on behalf of the American Dairy Science Association ®.This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

Page 2: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

Haematocrit (HCT)

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

Y4

X8

Kyn:TrpTrp

R123%stim

RBC

HCT

iC5%

Lactation

TMR TETMR SD

TMR Size

TMR ZC

TMR Freq

Page 3: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

Red Blood Cell count (RBC)

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

AK

AG3

P9

E8

RBC

HCT

Page 4: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

Lymfocyte count (LY)

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

AK

AH3

AG4

AG3

AE2

Z13

Z2

X4

J3

G12

G2

D4

GR

LY

Water Intake

WI Freq

CI Freq

Page 5: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

Monocyte count (MO)

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

R7

O1

N5N4

L3

E33

Haptoglobin

MO

Protein%

BCS

Page 6: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

Eosinophile count (EO)

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

U2

N9

N2

M1

L3

E2

GREO

C2/C3

Milk urea

TMR Freq

WI Size

CI TZ

Page 7: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

Granulocyte count (GR)

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

Z13

X4

T2

N2

E2

B1

AST

BilirubineGR

EO

LY

C3%

C2%

C2/C3

Page 8: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

CD4:CD8 ratio (CD Ratio)

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

AF

AD

Q

N2

L3

G6F

E33

E26

E22

E20

E2

CD8

CD Ratio

WI TD

WI SD CI SD

Page 9: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

CD8

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

AE3Z8

Q

M7

L4

L3

F

E22

E2

CD8

CD Ratio

BCSWI TD

Page 10: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

CD4

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

AJ

AH3AE1

Z5

P8

N6

E18

Shannon

CD4

FE1

FE2

CI TZ

Page 11: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

R123% stim

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

Z14

Y5

Y2

Y1

M1

L1

E35

E17

A

Urea

Kyn:Trp

R123+ Lym%

R123%stim

HCT

iC5%

CI SD

Page 12: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

R123 MFI stim

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

Z14

G7

GLDH

Protein

Kyn:Trp

SI

R123+ Lym%

R123 MFIstim

Milk urea

Lactation

BW

Page 13: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

R123% unst

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

AH2

AD

Z13

Z3

Y5

R1

H

G1E33

E20

E13

E2

B1

R123%unst

TMR TZ

Page 14: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

R123 MFI unst

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

X8

X7

X5

S

E33

E21E20 E16

E10 E7Chao1

species

AST

Urea

R123 MFIunst

R123 MFIstim

Milk urea

Page 15: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

R123+ Lym MFI

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

T2

P10

P6

P2

J1

G1

F

D4

R123+ Lym MFI

LPS

pH

Page 16: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

R123+ Lym%

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

Z15

Y2

Y1

G10

E26

C4

GLDH

R123+ Lym%

R123 MFIstim

R123%stim

C3%

C3

C2/C3

Page 17: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

SI

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

U3

T1

J2

G7

GLDH

SI

R123 MFIstim

C4

C3

C2

VFA

pH

Milk urea

Lactation

Page 18: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

AB stim

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

U2

T2

R6

R1

B1

ABunstim

ABstim

C3%

C3

C2%

CI Size

Page 19: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

AB unstim

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

AC

Z15

Z6

Z4

E28

E19

E8

ABunstim

ABstim

C3%

C2%

C2/C3

CI Size

TMR TE

CI ZC

Page 20: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

Tryptophan (Trp)

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

AC

AA

J3

E16

E9

B1

Kyn:TrpTrp

HCT

C5%

C5

C2

Protein yield

Protein%

Water Intake

CI SD

CI Total

TMR TETMR SD

TMR Size

Page 21: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

Kynurenine (Kyn)

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

AK

Z9

U4

P9

M2

L8

J3

E26

D3

Kyn:Trp

Kyn

BCS

Page 22: J. Dairy Sci. 101:4615–4637  ...Red Blood Cell count (RBC)-1.0 -0.5 0.0 0.5 1.0 PC1, variance explained: 12.04 %-1.0-0.5 0.0 0.5 1.0 d % 4 3 2 1 A B C D AK AG3 P9 E8 RBC HCT

Kyn:Trp ratio

-1.0 -0.5 0.0 0.5 1.0

PC1, variance explained: 12.04 %

-1.0

-0.5

0.0

0.5

1.0

PC

2, v

aria

nc

e e

xp

lain

ed

: 9.5

9 %

4

3

2

1

A B C D

Z15

Z4

X8

E4

A

Bilirubine

Urea

Kyn:Trp

Kyn

Trp

R123 MFIstim

R123%stim

HCT

BW

BCS

Water Intake

TMR Freq

TMR TETMR SD

TMR Size