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It’s All in the Kit: LoopSeq’s simple workflow delivers powerful performance using existing sequencing infrastructure

The power behind LoopSeq stems from Loop Genomics’s proprietary technologies:

� Sample preparation, which generates sequencing-ready libraries, includes a critical barcoding step, and enables unbiased single-molecule counting

� Assembly and analysis (performed on Loop Genomics’s cloud servers), which synthesizes the short-reads into single-molecule long-reads

See how LoopSeq Complete and LoopSeq Select take the complexity (and expense) out of complex microbial samples and deliver more accurate and quantitative 16S sequencing—visit LoopGenomics.com

LoopSeqTM CompleteLoopSeqTM Select

More accurate microbial metagenomicsEasy, streamlined workflows

Harnesses your current NGS system

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Next-generation sequencing (NGS) promised to revolutionize biology, and in many ways it has. With thousands of species whose whole genome has been completely sequenced and a myriad more partially done, we seem to be swimming in more sequence information than we know how to handle. But there are a number of critically important gaps that the promise of NGS has bypassed. 16S sequencing for microbial metagenomics studies is one. This is where Loop Genomics comes in.

Leveraging your existing sequencing infrastructure, Loop Genomics improves the accuracy of 16S sequencing with our LoopSeqTM Complete and LoopSeqTM Select products. With LoopSeq Complete and LoopSeq Select you get:

16S SequencingSimpler. Better. Cost-Effective.

Enhanced performance

� More comprehensive species identification � More accurate species classification � More accurate relative species abundance

A cost-effective solution

� Uses your existing short-read sequencing infrastructure � Requires no additional hardware � Saves time and labor with a simple, streamlined workflow

Powerful technology

� Converts your existing short-read sequencer into a long-read, single-molecule counting instrument

� Smartly implements synthetic long-read approach � Increases sequencing accuracy (error rate <0.005%)

LoopSeqTM Complete and LoopSeqTM Select provide different and complementary implementations of Loop Genomics’s powerful synthetic long-read sequencing technology, but the advantages are the same. Choose the one with the workflow that works best for you, and use the other product for additional validation.

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The simple LoopSeq workflow...

PhylumClass

Order

Species

Genus

Family

Sam

ple

Clas

sific

atio

n

40 %

20 %

80 %

60 %

100 %

0 %LoopSeq(V1-V9)

Illumina(V4-V5)

Illumina(V3)

SAMPLE

BARCODING LONG-READLIBRARY

PREPARATION

SEQUENCING

ASSEMBLY & ANALYSIS

DATA

INCLUDED WITH LOOPSEQ COMPLETE

The LoopSeq Complete workflow is simple, streamlined, and leverages your current short-read sequencing

instruments. We supply the reagents for barcoding and library preparation, you handle the sequencing.

After sequencing, simply upload your raw data to our cloud-based assembly and analysis pipeline and we’ll

return assembled long-reads in FASTA and FASTQ formats, plus a report which includes abundance and

classification of each molecule sequenced.

...leads to more accurate microbial metagenomics studies

16S sequencing analysis of a complex soil

sample comparing conventional short-read

sequencing (Illumina (V4-V5) and Illumina (V3))

versus LoopSeq Complete demonstrates the

power of LoopSeq for delivering a better, more

detailed picture of a microbial community.

With LoopSeq Complete, >99% of the unique

16S molecules were classified down to the

individual species or genus level, whereas the

conventional approach led to classification of

only ~65% of unique 16S molecules.