iTRAQ_p60_Tables_28.12.2010

7/28/2019 iTRAQ_p60_Tables_28.12.2010

1/14

Table 1. Functional classification of differentially expressed cC

ortex (Cx) proteins between wild- type and transgenic mouse quantified

by iTRAQ experiment

Protein ID Gene Symbol Protein Name Biological Process

No. of

Peptide

s

Tg Cx/WtCx

Subcellularlocation

Alzheimer Disease I suggest to make these sub headlines also in bold

IPI00230751 Ctnna2 Catenin alpha-2cellular component

morphogenesis11 1.355189443 Cytoplasmic

Parkinson Disease

IPI00131614 Sncb Beta-synuclein neurotransmitter secretion 5 0.679203629 Cytoplasmic

Huntington Disease

IPI00111117 Arpc5lActin-related protein 2/3 complex

subunit 5-like proteincell motion 2 1.458814263 Cytoplasmic

Chaperonic ResponseIPI00116308 St13 Hsc70-interacting protein immune system process 5 0.809095919 Cytoplasmic

IPI00126191 Lmnb2 Lamin-B2cellular component


GTPase Activity/G-protein/Kinases

IPI00224740 Pfn1 Profilin-1actin cytoskeleton

organization10 0.801678061 Cytoplasmic

IPI00263313 Drg1Developmentally regulated GTP-

binding protein 1

cytokinesis 2 1.22461617 Cytoplasmic

IPI00849279 Magi2

Membrane-associated guanylate

kinase, WW and PDZ domain-

containing protein 2

neurological system process 2 1.137627244Plasma

membrane

7/28/2019 iTRAQ_p60_Tables_28.12.2010

2/14

IPI00113521 Freq Neuronal calcium sensor 1 visual perception 2 0.895364761 ???

IPI00120374 Ppp2ca

Serine/threonine-protein

phosphatase 2A catalytic subunit

alpha isoform

immune system process 9 1.555965662 Cytoplasmic

IPI00467152 Gnai1guanine nucleotide binding protein,

alpha inhibiting 1

G-protein-coupled receptor

protein signaling pathway10 0.809095919

Plasma

membrane

Protein Metabolism

IPI00221581 Eif4bEukaryotic translation initiation

factor 4Bprotein metabolic process 2 0.870963573 Nuclear

IPI00124742 Eif4hEukaryotic translation initiation

factor 4Hprotein metabolic process 4 0.895364761 Cytoplasmic

IPI00330057 Denr Density-regulated protein protein metabolic process 2 0.870963573 Cytoplasmic

Microtubule-associated protein/Cytoskeletal protein , why is here more space on top/bottom of this headline ?

IPI00330862 Ezr Ezrincellular component


IPI00624843 Ermn Ermincellular component


IPI00263013 Plp1 Myelin proteolipid protein neurological system process 18 1.513561249Plasma

membran

IPI00229534 MarcksMyristoylated alanine-rich C-kinase

substrateintracellular signaling cascade 6 0.597035289 Nuclear

IPI00751009 Srpk2Serine/threonine-protein kinase

SRPK2immune system process 2 0.772680581 Cytoplasmic

IPI00867847 Kif2a Kinesin-like protein KIF2A intracellular protein transport 2 0.758577585 Cytoplasmic

IPI00131445 Dnm2 Dynamin-2 intracellular protein transport 22 1.19124198 Cytoplasmic

IPI00128973 Gap43 Neuromodulin axon choice point recognition 20 0.711213529 Nuclear

7/28/2019 iTRAQ_p60_Tables_28.12.2010

3/14

IPI00405986 Epb4.1l1 Erythrocyte protein band 4.1-like 1cortical actin cytoskeleton

organization8 0.847227395 Cytoplasmic

Anti-inflammatory Pprotein

IPI00317309 Anxa5 Annexin A5 intracellular protein transport 6 1.23594749 CytoplasmicCalcium ion binding protein

IPI00331066 Calb1 Calbindin intracellular signaling cascade 6 0.824138105 Cytoplasmic

IPI00123639 Calr Calreticulin protein metabolic process 11 0.679203629Endoplasmic

reticulum

IPI00467841 Calm1 Putative uncharacterized protein calcium ion binding 19 0.55462569 Cytoplasmic

IPI00474959 Rcn2 Putative uncharacterized protein calcium ion binding 3 0.847227395

Membranebound

Endoplasmicreticulum

Transport Pprotein

IPI00877254 Slc12a5 Solute carrier family 12 member 5synaptic vesicle/cation

transport10 1.258925438

Plasma

membrane

IPI00130455 Fxyd1 Phospholemman ion transport 3 1.202264428Plasma

membrane

IPI00420569 Atp1a2Sodium/potassium-transporting

ATPase subunit alpha-2cation transport 52 1.258925438

Plasma

membran

IPI00608015 Gria2 Glutamate receptor 2 neurological system process 7 1.270574093Plasma

membran

IPI00311682 Atp1a1 Sodium/potassium-transportingATPase subunit alpha-1

cation transport 48 1.380384207 Plasmamembrane

IPI00652882 Ffr 1110014N23Rik Isoform 1 of

Protein fat-free homolog

lipid and protein transport

transport3 1.137627244 Golgi

7/28/2019 iTRAQ_p60_Tables_28.12.2010

4/14

ReceptorAactivity

IPI00153990 Actr1b Beta-centractin intracellular protein transport 9 0.737904251 Cytoplasmic

Apoptosis signaling pathway and Cell cycle

IPI00130381 Bat3 Large proline-rich protein BAT3 apoptosis 4 0.879022539 Nuclear IPI00114667 Psmd7

26S proteasome non-ATPase

regulatory subunit 7protein metabolic process 3 1.127197504 Cytoplasmic

Mitochondrial P

proteins

IPI00120719 Cox5aCytochrome c oxidase subunit 5A,

mitochondrialoxidative phosphorylation 3 0.839460015 Mitochondrial

IPI00133562 Chchd3Coiled-coil-helix-coiled-coil-helixdomain-containing protein 3,

mitochondrial

4 0.737904251 Mitochondrial

Other Proteins

IPI00270326 Psmc2 Putative uncharacterized protein proteasome complex 2 0.64319998 Cytoplasmic

IPI00118022 Ap2s1 AP-2 complex subunit sigma-1intracellular proteintransport

2 1.29419589 Cytoplasmic

IPI00129519 Basp1 Brain acid soluble protein 1 neurological system process 22 0.363078058 Nuclear

IPI00118900 Pde1b

Calcium/calmodulin-dependent 3',5'-

cyclic nucleotide phosphodiesterase

1B

visual perception 4 0.895364761 Cytoplasmic

IPI00853932 Dmxl2 DmX-like protein 2intracellular protein

transport6 1.202628016 Cytoplasmic

IPI00321734 Glo1 Lactoylglutathione lyase metabolic process 3 1.445439816 Cytoplasmic

IPI00471441 Ptms Ptms proteinnucleotide and nucleic acid

metabolic process6 0.474241972 Nuclear

IPI00122131 Rin1 Ras and Rab interactor 1intracellular protein

transport;endocytosis2 0.704693079 Cytoplasmic

IPI00163011 Txndc5 Thioredoxin domain-containing protein metabolic process 2 1.629296064 Endoplasmic

7/28/2019 iTRAQ_p60_Tables_28.12.2010

5/14

protein 5 reticulum

IPI00828744 Krt10 Keratin, type I cytoskeletal 10cellular component


IPI00874497 Pde1aPhosphodiesterase 1A, calmodulin-dependent, isoform CRA_a

signal transduction 7 0.801678061 Cytoplasmic

IPI00474430 Sfrs2 Putative uncharacterized protein nucleic acid binding 5 0.751622915 Nuclear

IPI00758064 Psmc3Proteasome (Prosome, macropain)

26S subunit ATPase 3protein catabolic process 4 0.731139064 Cytoplasmic

IPI00874565 Nckap1 126 kDa proteinmaintenance of actin

cytoskeleton polarity8 1.116863251

Plasma

membrane

IPI00875877 Cd47 35 kDa proteinpositive regulation ofinflammatory response

5 1.247383475Plasmamembrane

IPI00648714 Eif5a 16 kDa protein Protein transport 4 0.679203629 Cytoplasmic

IPI00849626 LOC100046628 similar to Nucleophosmin - 3 0.704693079 Nuclear IPI00830456 Dpp10 85 kDa protein proteolysis 2 1.169499397 Membrane

IPI00653237 Wdr7 Wdr7 protein catalysis or binding 2 1.180320621Plasma

membrane

IPI00830290 Ptk2b Protein-tyrosine kinase 2-beta signal complex assembly 2 1.258925438 Cytoplasmic

IPI00918207 Anp32aAcidic leucine-rich nuclear

phosphoprotein 32 family member Aregulation of transcription 2 0.862978518 Nuclear

IPI00884509 Serbp1 mRNA binding protein 1 isoform 2 binding 2 0.280543357 Nuclear

IPI00761360 EG619937 similar to high-mobility group box 1 - 2 0.751622915 Nuclear

IPI00848506LOC100047971;10

0043740

hypothetical protein isoform 2 - 2 0.698232412 Cytoplasmic

7/28/2019 iTRAQ_p60_Tables_28.12.2010

6/14

Table 2. Functional classification of differentially expressed hHippocampus (HP) proteins between wild type and transgenic mouse

quantified by iTRAQ experiment

Protein ID Gene Symbol Protein Name Biological ProcessNo. of

PeptidesTg Hp/ Wt Hp

Subcellular

location

AlzheimerDdisease

IPI00116498 Ywhaz 14-3-3 protein zeta/delta cell cycle; 56 0.847227395 Cytoplasmic

Parkinson Ddisease

IPI00169925 Ndufv2

NADH dehydrogenase

[ubiquinone] flavoprotein 2,

mitochondrial

apoptosis 10 4.017908096 Mitochondrial

Huntington Ddisease

IPI00112251 Tubb3 Tubulin beta-3 chainintracellular protein

transport;137 0.772680581 Mitochondrial

IPI00115663 Dctn1 Dynactin subunit 1intracellular proteintrans

port12 1.247383475 Cytoplasmic

IPI00122094 Dlg4 Disks large homolog 4neurological system

process;16 0.787045777

Plasma

membrane

Chaperonic RResponse

IPI00331556 Hspa4 Heat shock 70 kDa protein 4 immune system process 18 0.824138105 Cytoplasmic

IPI00116498 Ywhaz 14-3-3 protein zeta/delta cell cycle; 56 0.847227395 Cytoplasmic

GTPase Aactivity/G-Pprotein/Kinases

7/28/2019 iTRAQ_p60_Tables_28.12.2010

7/14

7/28/2019 iTRAQ_p60_Tables_28.12.2010

8/14

Ubiquitin Pproteasome

IPI00402913 Ube2v2Ubiquitin-conjugating enzyme

E2 variant 2apoptosis 6 1.380384207 Mitochondrial

IPI00608097 NpeppsAminopeptidase puromycin

sensitive proteolysis 17 0.779830098 Cytoplasmic

Protein Metabolism

IPI00221581 Eif4bEukaryotic translation initiation

factor 4Bprotein metabolic process 2 1.258925438 Nuclear

Microtubule-Aassociated Pprotein/Cytoskeletal Pprotein

IPI00330862 Ezr Ezrincellular componentmorphogenesis


IPI00323800 NefmNeurofilament medium

polypeptide

cellular component


IPI00459442 Rtn1 reticulon 1 isoform RTN1-C neuron differentiation 3 0.809095919

Endoplasmic

reticulum

IPI00124954 Kif5b Kinesin-1 heavy chainintracellular protein


IPI00454081 Kif21a Kinesin-like protein KIF21Aintracellular protein


IPI00111117 Arpc5lActin-related protein 2/3 complexsubunit 5-like protein

cell motion 2 0.613762021 Cytoplasmic

IPI00459725 Idh3aIsocitrate dehydrogenase [NAD]

subunit alpha, mitochondrialtricarboxylic acid cycle 12 0.779830098 Mitochondrial

IPI00229510 Ldhb L-lactate dehydrogenase B chain tricarboxylic acid cycle 31 0.809095919 Cytoplasmic

IPI00130589 Sod1 Superoxide dismutase [Cu-Zn] oxidation reduction 11 1.47231245 CytoplasmicTransport protein



Plasma

membrane

7/28/2019 iTRAQ_p60_Tables_28.12.2010

9/14



Plasma

membrane

IPI00877254 Slc12a5Solute carrier family 12 member

5cation transport 10 0.824138105

Plasma

membrane

IPI00323571 Apoe Apolipoprotein E lipid transport 8 1.282330632Extracellular

(Secreted)

IPI00121576 Apoo Apolipoprotein O lipid transport 3 2.91071701Plasma

membrane

IPI00230553 Fxyd6FXYD domain-containing ion

transport regulator 6ion transport 3 0.801678061

Plasma

membrane

DNA-B binding

IPI00757097 Shank2SH3 and multiple ankyrin repeat

domains protein 2

cell surface receptor linked

signal transduction4 0.824138105 Nuclear

IPI00113149 Stx1b Syntaxin-1B

intracellular protein

transport, neurotransmittertransport

36 0.809095919Plasma

membrane

IPI00649135 Gstm1 Glutathione S-transferase, mu 1 transferase activity 26 0.816582382 Cytoplasmic

IPI00652882 1110014N23RikIsoform 1 of Protein fat-free

homologlipid transport 3 0.801678061 Golgi

IPI00378063 Ap2b1 AP-2 complex subunit beta-1intracellular protein


IPI00319933 Pacsin3Protein kinase C and casein

kinase II substrate protein 3

intracellular signaling

cascade2 1.406047583 Cytoplasmic

IPI00415402 Stxbp1 Syntaxin-binding protein 1 neurotransmitter secretion 71 0.737904251 Nuclear

IPI00469548 Syn2 Synapsin-2 neurological systemprocess 51 0.794328213 Cytoplasmic

IPI00109727 Thy1 Thy-1 membrane glycoprotein signal transduction 9 0.816582382Plasma

membrane

Calcium- Iion- Bbinding Pprotein

7/28/2019 iTRAQ_p60_Tables_28.12.2010

10/14

IPI00331066 Calb1 Calbindinintracellular signaling

cascade6 1.367728829 Cytoplasmic

IPI00758006 2900073G15Rik Putative uncharacterized protein calcium ion binding 3 1.213388801 Cytoplasmic

Hydrolase Aactivity

IPI00154004 Otub1 Ubiquitin thioesterase OTUB1 protein metabolic process 13 0.839460015 Cytoplasmic

IPI00403586 Aadacl1 Arylacetamide deacetylase-like 1 protein dephosphorylation 4 1.22461617

Membrane

-bound

Extracellular

IPI00407158 Srr Serine racemaseneurological system

process4 0.809095919 Cytoplasmic

RNA Hhelicase and RNA Ssplicing Ffactor Aactivity

IPI00317794 Ncl Nucleolin spermatogenesis 8 0.772680581 Nuclear

IPI00129430 SfpqSplicing factor, proline- and

glutamine-richspermatogenesis 4 0.787045777 Nuclear

IPI00128904 Pcbp1 Poly(rC)-binding protein 1 neurological system proces 9 1.406047583 Cytoplasmic

Other Proteins

IPI00918129 Pde2aphosphodiesterase 2A, cGMP-

stimulated isoform 1signal transduction 4 1.270574093

Plasma

membrane

IPI00758064 Psmc3Proteasome (Prosome,macropain) 26S subunit ATPase

3

blastocyst development 4 1.29419589 Cytoplasmic

IPI00757312 Myh10Myosin, heavy polypeptide 10,

non-musclecardiac myofibril assembly 39 0.711213529

Plasma

membrane

IPI00775829 Pklr pyruvate kinase, liver and RBC

isoform 2glycolysis 2 1.499684811 RA

7/28/2019 iTRAQ_p60_Tables_28.12.2010

11/14

IPI00387312 Pfklphosphofructokinase, liver, B-

type

carbohydrate metabolic

process10 0.824138105 Cytoplasmic

IPI00407863 Clasp2 CLIP-associating protein 2 mitosis 6 0.660693467 Nuclear

IPI00380436 Actn1 Alpha-actinin-1 ??? find out??? find out 33 0.794328213 Cytoplasmic

IPI00465812 Epb41l1 Band 4.1-like protein 1 ??? find out 14 0.895364761 Cytoplasmic

IPI00667973 Cep170 Centrosomal protein of 170 kDa ??? find out 2 0.855066717 Cytoplasmic

IPI00169622 PhyhipPhytanoyl-CoA hydroxylase-interacting protein

??? find out 4 0.847227395 Cytoplasmic

IPI00230061 Plec1 Plectin-1 ??? find out 48 0.855066717 Cytoplasmic

IPI00125960 Ndrg1 Protein NDRG1 ??? find out 3 1.905460715 Cytoplasmic

IPI00319830 Sptbn1 Spectrin beta chain, brain 1 ??? find out 134 0.839460015 Cytoplasmic

IPI00622235 VcpTransitional endoplasmic

reticulum ATPase


transport38 0.591561615

Membrane

boundMitochondrial

IPI00120030 Crym Mu-crystallin homolog

cellular amino acid and

derivative metabolic

process


Mitochondrial P proteins (Respiratory Chain) why empty line below still????

IPI00132728 Cyc1Cytochrome c1, heme protein,


IPI00313841 Atp6v0d1V-type proton ATPase subunit d

1cation transport 15 0.801678061 Mitochondrial

IPI00117978 Cox4i1 Cytochrome c oxidase subunit 4isoform 1, mitochondrial

oxidative phosphorylation 12 2.466039419 Mitochondrial

IPI00121288 Ndufb10 NADH dehydrogenase

[ubiquinone] 1 beta subcomplex


7/28/2019 iTRAQ_p60_Tables_28.12.2010

12/14

subunit 10

IPI00126085 Cyp46a1 Cytochrome P450 46A1respiratory electron

transport chain3 0.758577585 Mitochondrial

IPI00116748 Ndufa10NADH dehydrogenase[ubiquinone] 1 alpha subcomplex

subunit 10, mitochondrial


MPT Pore

IPI00122547 Vdac2Voltage-dependent anion-

selective channel protein 2anion transport 16 0.731139064 Mitochondrial

Amino Aacid Ttransporter

IPI00230754 Slc25a11

Mitochondrial 2-

oxoglutarate/malate carrier

protein

cation transport 14 0.824138105 Mitochondrial

IPI00336324 Mdh1 Malate dehydrogenase,cytoplasmic

tricarboxylic acid cycle 28 0.831763744 Cytoplasmic

IPI00406442 Suclg1

Succinyl-CoA ligase [GDP-

forming] subunit alpha,

mitochondrial

tricarboxylic acid cycle 7 0.839460015 Mitochondrial

IPI00132042 Pdhb

Pyruvate dehydrogenase E1

component subunit beta,

mitochondrial

vitamin biosynthetic

process33 0.794328213 Mitochondrial

IPI00132728 Cyc1Cytochrome c1, heme protein,


IPI00117312 Got2 Aspartate aminotransferase,mitochondrial


derivative metabolicprocess

50 0.816582382 Mitochondrial

IPI00464317 Gls glutaminase isoform 1glutamine metabolic

process14 2.421029091 Mitochondrial

7/28/2019 iTRAQ_p60_Tables_28.12.2010

13/14

IPI00911135 Oxr1 oxidation resistance 1 isoform Ecell wall macromolecule

catabolic process19 0.824138105 Mitochondrion

OtherPproteins

IPI00467841 Calm1 Putative uncharacterized protein ??? find out 19 1.282330632 Cytoplasmic

IPI00666196 LOC631033similar to lysyl-tRNA synthetase

isoform 12 0.672976673

NA find out I

told you to fnd

out, so please do

so!

IPI00751137 Tomm70a Putative uncharacterized p rotein ??? find out 6 1.23594749Membrane-bound

Mitochondrial

IPI00761360 EG619937similar to high-mobility groupbox 1

2 2.466039419 Nuclear

IPI00785371 L1cam L1 cell adhesion molecule ??? find out 3 1.923091769 NA ??? find outIPI00808053 Ccdc136

hypothetical protein LOC232664

isoform 1??? find out 2 0.809095919 NA ??? find out

IPI00881320 Brsk1 77 kDa protein ??? find out 2 1.29419589 Cytoplasmic

IPI00881347 Hpca 22 kDa protein ??? find out 11 1.905460715Plasma

membrane

IPI00882364 Ndufb5 20 kDa protein ??? find out 3 2.128139019 NA find out

IPI00884509 Serbp1mRNA binding protein 1 isoform

2??? find out 2 1.355189443 Nuclear

IPI00889817 Ddx19a Ddx19-like protein ??? find out 3 1.330454469 Cytoplasmic

IPI00890268 Myo5a 215 kDa protein ??? find out 4 0.779830098 NA, find out

IPI00918129 Pde2aphosphodiesterase 2A, cGMP-stimulated isoform 1

signal transduction 4 1.270574093Plasmamembrane

IPI00758064 Psmc3 Proteasome (Prosome,macropain) 26S subunit ATPase

blastocyst development 4 1.29419589 Cytoplasmic

7/28/2019 iTRAQ_p60_Tables_28.12.2010

14/14

3

IPI00757312 Myh10Myosin, heavy polypeptide 10,

non-musclecardiac myofibril assembly 39 0.711213529

Plasma

membrane

IPI00775829 Pklr Pyruvate kinase, liver and RBCisoform 2

glycolysis 2 1.499684811 RA???

IPI00387312 PfklPhosphofructokinase, liver, B-

type

carbohydrate metabolic

process, carbohydrate

phosphorylation


IPI00407863 Clasp2 CLIP-associating protein 2 mitosis 6 0.660693467 Nuclear

IPI00465812 Epb41l1 Band 4.1-like protein 1 14 0.895364761 Cytoplasmic

IPI00667973 Cep170 Centrosomal protein of 170 kDa ??? find out 2 0.855066717 Cytoplasmic

IPI00169622 PhyhipPhytanoyl-CoA hydroxylase-

interacting protein??? find out 4 0.847227395 Cytoplasmic

IPI00230061 Plec1 Plectin-1 ??? find out 48 0.855066717 CytoplasmicIPI00125960 Ndrg1 Protein NDRG1 ??? find out 3 1.905460715 Cytoplasmic

IPI00319830 Sptbn1 Spectrin beta chain, brain 1 ??? find out 134 0.839460015 Cytoplasmic

IPI00622235 VcpTransitional endoplasmic

reticulum ATPase


transport38 0.591561615 Mitochondrial

IPI00120030 Crym Mu-crystallin homolog


derivative metabolic

process


iTRAQ_p60_Tables_28.12.2010

Documents

Transcript of iTRAQ_p60_Tables_28.12.2010