Isolation & identification of staphylococcus auerus

12
ISOLATION & IDENTIFICATION OF STAPHYLOCOCCUS AUREUS

Transcript of Isolation & identification of staphylococcus auerus

Page 1: Isolation & identification of staphylococcus auerus

ISOLATION & IDENTIFICATION OF STAPHYLOCOCCUS AUREUS

Page 2: Isolation & identification of staphylococcus auerus
Page 3: Isolation & identification of staphylococcus auerus

AIM: To isolate and identify bacterial pathogen: Staphylococcus aureus

Page 4: Isolation & identification of staphylococcus auerus

MATERIALS REQUIRED: 1. SAMPLE: UNKNOWN SAMPLE. 2. MEDIA: NUTRIENT AGAR, MANNITOL SALT AGAR, MACCONKEY AGAR. 3. REAGENTS: CRYSTAL VOILET, GRAM’S IODINE, ETHANOL, SAFFARNIN, HYDROGEN PER OXIDE SOLUTION. 4, GLASSWARES & EQUIPMENTS: GLASS SLIDES, TEST TUBES, PETRIDISHES, BUNSEN BURNER, ph METER,FILTER PAPER, PRESSURE COOKER, HOT AIR OVEN, ERLEN MEYER FLASK.

Page 5: Isolation & identification of staphylococcus auerus

PROCEDURE: STAINING [GRAM’S STAINING]

MACCONKEY AGAR PLATES

SLIDE & TUBE COAGULASE TEST

GELATIN LIQUIFICATION

Page 6: Isolation & identification of staphylococcus auerus

MANNITOL SALT AGAR

BACTERICIN SENSITIVITY TEST

Page 7: Isolation & identification of staphylococcus auerus

OBSERVATIONS:1. GRAM’S STAINING:

Page 8: Isolation & identification of staphylococcus auerus

2. MACCONKEY AGAR PLATES:

Page 9: Isolation & identification of staphylococcus auerus

3. SLIDE AND TUBE COAGULASE TEST:

Plasma of human is taken the slide.Its is mixed well with the culture.Then observe for clump formation.

Page 10: Isolation & identification of staphylococcus auerus

4. GELATIN LIQUIFICATION TEST:

Page 11: Isolation & identification of staphylococcus auerus

5.MANNITOL SALT AGAR:

Page 12: Isolation & identification of staphylococcus auerus

6.BACITRACIN SENSITIVITY TEST:


Isolation and Identification of Vancomycin Resistant Staphylococcus

Isolation and Identification of Vancomycin Resistant Staphylococcus

Isolation, Identification and Antimicrobial Sensitivity ...docs.neu.edu.tr/library/6365608543.pdf · Isolation, Identification and Antimicrobial Sensitivity ... Identification and

Isolation, Identification and Antimicrobial Sensitivity ...docs.neu.edu.tr/library/6365608543.pdf · Isolation, Identification and Antimicrobial Sensitivity ... Identification and

Isolation, identification and biochemical characterization ...

Isolation, identification and biochemical characterization ...

Isolation of Staphylococcus epidermidis from inflamed upper ...Isolation of Staphylococcus epidermidis from inflamed upper respiratory tract of an orange-spined hairy dwarf porcupine

Isolation of Staphylococcus epidermidis from inflamed upper ...Isolation of Staphylococcus epidermidis from inflamed upper respiratory tract of an orange-spined hairy dwarf porcupine

Isolation and Identification of

Isolation and Identification of

Isolation & Identification of Enterobacteriaceae & Pseudomonas

Isolation & Identification of Enterobacteriaceae & Pseudomonas

Isolation and Identification of Staphylococci

Isolation and Identification of Staphylococci

Isolation of a Pigment-producing Strain of Staphylococcus kloosii ...

Isolation of a Pigment-producing Strain of Staphylococcus kloosii ...

GENUS STAPHYLOCOCCUS: Identification of Speciesdelrio.dcccd.edu/jreynolds/microbiology/2421/lab_manual/StaphID.pdf · GENUS STAPHYLOCOCCUS: Identification of Species Staphylococcus

GENUS STAPHYLOCOCCUS: Identification of Speciesdelrio.dcccd.edu/jreynolds/microbiology/2421/lab_manual/StaphID.pdf · GENUS STAPHYLOCOCCUS: Identification of Species Staphylococcus

ISOLATION, IDENTIFICATION AND TECHNOLOGICAL …

ISOLATION, IDENTIFICATION AND TECHNOLOGICAL …

Phytochemicals: Extraction, Isolation, and Identification ...

Phytochemicals: Extraction, Isolation, and Identification ...

Identification of the Immunodominant Regions of Staphylococcus … · 2018. 11. 21. · Identification of the Immunodominant Regions of Staphylococcus aureusFibronectin-Binding Protein

Identification of the Immunodominant Regions of Staphylococcus … · 2018. 11. 21. · Identification of the Immunodominant Regions of Staphylococcus aureusFibronectin-Binding Protein

44179573 Isolation Identification of Microbes

44179573 Isolation Identification of Microbes

Identification of Staphylococcus aureus Colony-

Identification of Staphylococcus aureus Colony-

Isolation, Characterization and Identification Of

Isolation, Characterization and Identification Of

vicK Gene as Potential Identification Marker for ...vicK Gene, Staphylococcus aureus, Identification Marker, Rapid Diagnostic, Two-Component Signal Transduction 1. Introduction Staphylococcus

vicK Gene as Potential Identification Marker for ...vicK Gene, Staphylococcus aureus, Identification Marker, Rapid Diagnostic, Two-Component Signal Transduction 1. Introduction Staphylococcus

Research Paper Isolation and Identification of miRNAs in ...free-journal.umm.ac.id/files/file/Isolation and Identification of... · Isolation and Identification of miRNAs in Jatropha

Research Paper Isolation and Identification of miRNAs in ...free-journal.umm.ac.id/files/file/Isolation and Identification of... · Isolation and Identification of miRNAs in Jatropha

Isolation and Identification of Vancomycin Resistant ... · Isolation and Identification of Vancomycin Resistant Staphylococcus aureus from Post ... latex agglutination reagent, chloroform:isoamyl

Isolation and Identification of Vancomycin Resistant ... · Isolation and Identification of Vancomycin Resistant Staphylococcus aureus from Post ... latex agglutination reagent, chloroform:isoamyl

ISOLATION, IDENTIFICATION AND MOLECULAR …

ISOLATION, IDENTIFICATION AND MOLECULAR …

ISOLATION AND IDENTIFICATION OF CAUSAL DISEASE OF ... and Identification of Causal Disease of... · ISOLATION AND IDENTIFICATION OF CAUSAL DISEASE OF . ... Fungi and Identification

ISOLATION AND IDENTIFICATION OF CAUSAL DISEASE OF ... and Identification of Causal Disease of... · ISOLATION AND IDENTIFICATION OF CAUSAL DISEASE OF . ... Fungi and Identification