enrichment isolation cultivation and preservation of bacteria
Isolation and Mass Cultivation of Pigment Producing Bacteria
Transcript of Isolation and Mass Cultivation of Pigment Producing Bacteria
ISOLATION AND MASS CULTIVATION OF PIGMENT PRODUCING BACTERIA
INTENT:y To Isolate the Organism from the Source y To Purify and Obtain Axenic Culture y Maintenance of Axenic Culture y Characterization of the Organism(Physiological&
Biochemical) y Mass Multiplication of the Organism y Pigment Extraction(Solvent Extraction)
ISOLATION:y Source(Rhizosphere-Soil-1gm) y Serial Dilution y 10 -5 and 10-6
Kings B base Medium y Streak and Pour Plate y RESULT: Orange Coloured Colonies(Round, Raised, Mucoid )
PURIFICATION:y Single Colony Transferred in to Nutrient Agar y Quadrant Streaking y Single Colony to NA Slant
MAINTENANCE:y Colonies were Transferred to New NA Slants Every Two
Weeks and Maintained
CHARACTERIZATION:y PHYSIOLOGICAL(Gram ve, Rod, Non Motile) y BIOCHEMICAL(Catalase ve, Oxidase ve,
TSI test-Pink Colonies, Citrate ve, Indole Test +ve)
MASS CULTIVATION:y Pure Colonies From NA Slant y Inoculated in 250 ml of Nutrient Broth y Incubated in Rotary Shaker for Five Days at 37 degree