ISAAC CARILO (MPhil Student)-Molecular Cell Biology of … · 2017. 8. 3. · ISAAC CARILO (MPhil...
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ISAAC CARILO(MPhil Student)-Molecular Cell Biology of Infectious Diseases
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GEOGRAPHICAL LOCATION
DEPARTMENTOFBIOCHEMISTRY,CELLANDMOLECULARBIOLOGY,UNIVERSITYOFGHANA
WORLDMAP
GHANA
ACCRA
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LONG TERM SCIENTIFIC GOALS• Elucidation of drug resistance/stress response mechanisms
in infectious pathogens confronting the Sub-Saharan Africa.
• Discovery of novel therapeutic agents for infectious diseases. Eg. Tuberculosis, Malaria, Candidiasis, Bacterial infections
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Laboratory for Chemical Systems Biology of Infectious Pathogens (LCSBIP).
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RESEARCH QUESTIONS AT LAB.• Elucidating drug resistance/stress response mechanisms of
infectious pathogens at the system-wide level using mass spectrometry-based proteomics
− Mycobacterium tuberculosis, bacterial infections− Candida albicans, Streptomyces cerevisae− Plasmodium falciparum
• Bioassay-guided isolation of novel next generation bioactive compounds from fungal sources
− Marine Endophytic fungi− Wood decay fungi− Soil Borne Fungi− Terrestrial endophytic fungi
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RESEARCHQUESTION(MPhilPROJECT)
• Investigations into the mechanisms of anti-mycobacterial drug resistance using antipsychotic compounds.
• Unique features of the Laboratory− Largest collection of fungal cultures
in Ghana− Active extracts with cross bioactivity
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Tubercle bacilli
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UNIQUESKILLSACQUIRED• Extraction of secondary metabolites from fungal cultures• Microbiology techniques (culturing, transformation etc)
• Kupchan Solvent partitioning− Partitioning of extracts into 7 different fractions
• Preparative and Analytical Thin layer Chromatography (TLC)
− For sub-fractionations of extracts
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CHALLENGES WITH RESERACH• Ultra-high throughput screening technologies.
• Need for LC systems and liquid equipment and complete set up for separation of organic compounds
• Availability of equipment for system-wide analysis of stress-induced organisms (eg. Mass spectrometry).
• Structural resolution and quantification techniques of extracts (Nuclear magnetic Resonance, HPLC)
• Space7/24/17 7
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COLLABORATION• State-of-the-art high throughput screening
• Current trends in structural elucidation approaches and techniques
• Training and innovation
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ACKNOWLEDGEMENTS• Superb contributions from MeBoP
course coordinators and organizers.
• Home institution (Department of Biochemistry, Cell and Molecular Biology, University of Ghana)
• Peer Groups of MeBoP course, 2017,
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THANK YOU FOR YOUR ATTENTION
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