colu

mns I o n P a c C S 1 6 C a t i o n - E x c h a n g e C o l u m n

Passion. Power. Productivity.

The IonPac® CS16 cation-exchange column is designed for the determination of disparate concentra-tion ratios of sodium and ammonium (up to 10,000:1) in diverse sample matrices using an isocratic acid eluent and elevated temperature. Other impor-tant applications include the determi-nation of short-chained alkylamines and alkanolamines in the presence of lithium, sodium, ammonium, potassium, magnesium, and calcium in complex sample matrices. The IonPac CS16 col-umn is recommended for most IonPac CS15 applications. The column selectiv-ity is optimized for a 40 °C operating temperature for maximum peak effi-ciencies. Solvent compatibility permits easy column clean-up after the analysis of complex matrices. Formats avail-able include 0.5 mm, 3 mm, and 5 mm, allowing use of Capillary to analytical flow rates and supporting advanced IC × IC applications.

Superior Chromatographic Performance• Universalcolumnfordisparate

concentrationratiosofammoniumandsodiumincomplexsamplematrices

• CS16Capillarycolumnoffersreducedeluentconsumptionandreducedoperatingcosts

• Tracelevelammoniuminhighcon-centrationsofsodium;tracelevelsodiuminhighconcentrationsofammoniumoramines

• Determineamines,includingalkylaminesandalkanolaminesindiversesamplematrices

• DesignedtobeusedinmostIonPacCS15applicationswithalargerlinearworkingrange

• ImprovedlowsamplepHtolerance

• Highcapacity:8400µeqper column(5×250mmcolumn)

• Fourfoldand100-foldincreaseinmasssensitivity,respectively,with 3mmi.d.microboreand0.5mmi.d.capillaryformats

• Columnselectivityoptimizedfora40°Coperatingtemperatureformaximumpeakefficiencies

• SimplifiedoperationprovidedbytheEluentGenerator,which requiresonlyadeionizedwatersourcetoproducemethanesulfonicacid(MSA)eluent

• Compatiblewithorganicsolventstoenhanceanalytesolubility,tomodifycolumnselectivity,orforeffectivecolumnclean-up

Figure 2. Isocratic separation of common inorganic cations and ammonium.

Figure 1. Structure of an IonPac CS16-5µm packing particle.

• Samplematricesincludeenviron-mentalwatersandsoilextracts;powerplantwaterstreatedwithammonium,morpholine,orethanol-amine;chemicaladditives;chemicalprocesssolutions;scrubbersolu-tions;platingbaths;andsolvents

Unique Carboxylate Cation ExchangerTheIonPacCS16columnisa

uniquehydrophilic,highcapacitycar-boxylatefunctionalizedcationexchang-erthatprovidesexcellentpeakshapeforalkaliandalkalineearthmetalsandamines.TheIonPacCS16columnpack-ingisa5-µmdiametermacroporousparticleconsistingofethylvinylbenzenecrosslinkedwith55%divinylbenzeneasshowninFigure1.Thesubstrateisfunc-tionalizedwithahydrophiliccarboxylicacidlayerthatpermitstheelutionofmono-anddivalentcationsusingadilutehydroniumioneluent,suchasMSAorsulfuricacid.TheCS16packingisanewadvancementinDionexresintech-nology.Theuseofasmallerresinpar-ticlesize(5µm)providestheadvantageofimprovedpeakefficiencies,whilethenewgraftingtechnologyallowshighercapacitythroughtheincorporationofamuchlargernumberofcarboxylicacidcation-exchangesites.

Improved ResolutionTheIonPacCS16columnpro-

videsimprovedresolutionamongtheanalytepeaksduetoitshighercation-exchangecapacity.Itwasdesignedspecificallyforhigh-to-lowratiosofso-diumandammoniumindiversesamplematrices.Ratiosofupto10,000:1canberesolvedusingasimpleisocraticacidiceluentatanelevatedtempera-ture(40˚C)coupledwithsuppressedconductivitydetection.Thecommoninorganiccationsandammoniumcanberesolvedin20minutesusing30mMMSAasshowninFigure2.

TheMSAorsulfuricacidcon-centrationcanbeoptimizedforthefastdeterminationofGroupIandIIcationsusing48mMMSA,alsoillustrated inFigure2.

Macroporous Polymeric Substrate

Surface Area = 450 m2/g

5 nm

16396

2.5 ∝m

CO2-CO2-

CO2-

CO2-

CO2-

CO2-

CO2-

CO2-

CO2-

CO2-

CO2-CO2-

16170Minutes

Minutes

0 5 10 15 20 25

0

1.30

1 23

45 6

µS

0 10

0

1.301

2 3 5 4 6

µS

Column: IonPac CS16 (5 × 250 mm)Eluent: See chromatogramFlow Rate: 1.0 mL/minInj. Volume: 25 L Temperature: 40 °CDetection: Suppressed conductivity, CSRS® -ULTRA 4 mm, AutoSuppression® recycle mode

Peaks: 1. Lithium 0.1 mg/L (ppm) 2. Sodium 0.4 3. Ammonium 0.5 4. Potassium 1.0 5. Magnesium 0.5 6. Calcium 1.0

30 mM Methanesulfonic acid

48 mM Methanesulfonic acid

5

Figure 3. Separation of six cations on a capillary CS16 column.

28016

Minutes

Column: IonPac CS16 (0.5 × 250 mm)Eluent Source: Capillary EGC-MSA cartridge Eluent: 30 mM MSAFlow Rate: 10 µL/minTemperature: 40 °CSuppressor: CCES™ 300 Cation Capillary Electrolytic Suppressor Detection: Suppressed conductivity Injection Volume: 0.4 µL

Peaks: 1. Lithium 2. Sodium 3. Ammonium 4. Potassium 5. Magnesium 6. Calcium

0 4 8 12 16 20 24-0.2

2.5

µS

12 3 4

5 6

CS16 Capillary FormatTheCS16Capillarycolumn

(0.5×250mm)ispackedwiththesamematerialastheequivalentanalyticalscaleversion,thusproducingthesameperformanceasa5mmcolumn,butre-quiresonly1/100ththeeluentflowrate. Thecapillaryformatofferstheadvan-tageoflesseluentconsumptionprovid-ingreducedoperatingcosts.Figure3illustratestheseparationofthecommoninorganiccationsusingtheCS16Capil-laryColumn.Excellentretentiontimereproducibilitycanbeachievedwiththecapillaryformat.

High Loading Capacity for Trace Level Ammonium in High Sodium Samples

TheIonPacCS16isahighcapac-itycation-exchangecolumnthatreplacestheCS15columnfordisparateconcen-trationratiosofammoniumandsodiumindiversesamplematrices.TheCS16isidealforthedeterminationoflowconcentrationsofammoniuminenvi-ronmentalwaters.Itprovidesimprovedresolutionofsodiumfromammoniumandalkanolamines,evenforsampleshighinionicstrength.Figure4Aillustratesthedeterminationoftracelevelammoniuminthepresenceofhighsodiumatratiosupto10,000:1usinganisocraticMSAeluentontheCS165mmcolumn.Ratiosupto20,000:1canberesolvedusingaMSAgradientwiththeCS163mmcol-umnasillustratedinFigure4B.

Figure 4. Determination of trace level ammonium in a high sodium sample.

17493

0

0

0.08

2µS

0.40

µS

1

0 4 6 82 10

A

B

Column A: IonPac CS16 (5 × 250 mm)Column B: IonPac CS16 (3 × 250 mm)Eluent A: 30 mM Methanesulfonic acidEluent B: 7 mM Methanesulfonic acid for 23 min to 52 mM at 28 minFlow Rate A: 1.0 mL/minFlow Rate B: 0.43 mL/min Temperature: 40 °CInj. Volume: 25 L Detection: Suppressed conductivity, CSRS-ULTRA, AutoSuppression recycle mode

Peaks A: 1. Sodium 100 mg/L (ppm) 2. Ammonium 0.01

Peaks B: 1. Sodium 500 2. Ammonium 0.025

CTC-1 Column placed between the Cell Out and Regen In Ports

2

1

0 5 10 15 20Minutes

Minutes

Figure 5. Determination of trace level sodium in a high ammonium sample.

High Loading Capacity for Trace Level Sodium in High Ammonium Samples

TheIonPacCS16columnisalsoidealforthedeterminationoftracelevelsodiumatng/L(ppt)concentrationsinammoniumandamine-treatedcoolingwaters.ThehighcapacityanduniqueselectivityoftheCS16columnallowtheresolutionoftracesodiuminthepresenceofhighlevelsofammoniumandalkanolamines.Figures5 and6illustratethedeterminationoftracecon-centrationsofsodiuminthepresenceofhighlevelsofammoniumoramines.

Figure 6. Determination of trace level sodium in a high amine sample.

Column: IonPac CS16 (5 × 250 mm)Eluent: 30 mM Methanesulfonic acidTemperature: 40 °CFlow Rate: 1.0 mL/minInj. Volume: 25 LDetection: Suppressed conductivity, CSRS-ULTRA 4 mm, AutoSuppression recycle mode

Peaks: 1. Sodium 0.01 mg/L (ppm) 2. Ammonium 100

16391

0 5 10 15 20 25Minutes

0.07

µS

0

1

2

CTC-1 Column placed between Cell Out and Regen In Ports

Minutes

2.7

µS

0

1

23

5 76 8

4

0 5 10 15 20 25

17494

30 35

Column: IonPac CS16 (3 × 250 mm)Eluent: 7 mM Methanesulfonic acid for 23 min to 52 mM at 28 minTemperature: 40 °CFlow Rate: 0.43 mL/minInj. Volume: 25 L Detection: Suppressed conductivity, CSRS-ULTRA 2 mm, AutoSuppression recycle mode

Peaks: 1. Lithium 2500 g/L (ppb) 2. Sodium 6 3. Ammonium 100 4. Ethanolamine 2000 5. Hydrazine 4000 6. Potassium 4 7. Magnesium 1 8. Calcium 6

CTC-1 Column placed between the Cell Out and Regen In Ports

Figure 7. Determination of methyl amines and the Group I and II cations using the IonPac CS16 column. The CSRS-ULTRA was operated outside the heated column compartment.

Figure 8. Determination of alkanolamines and the Group I and II cations using the IonPac CS16 column. The CSRS-ULTRA was operated outside the heated column compartment.

Determination of Amines in Complex MatricesTheIonPacCS16columncanbeusedtomonitortheaminecontentinthequalitycontrolofchemicaladditives,processsolutions,platingbaths,andscrubbersolutions.TheCS16column isidealforthedeterminationofaminesinavarietyofsamplematrices.Figures7and8illustratetheseparationofshort-chainedhydrophilicmethylaminesandalkanolaminesandGroupIandIIcat-ionsinasinglerun.Forspecificamineapplications,theeluentconditionscanbeoptimizedtoresolvetheaminesofinter-estandthecommoninorganiccations.

Column: IonPac CS16 (3 × 250 mm)Eluent: 20 mM Methanesulfonic acidEluent Source: EG40Flow Rate: 0.5 mL/minInj. Volume: 25 LTemperature: 60 °CDetection: Suppressed conductivity, CSRS-ULTRA 2 mm, AutoSuppression recycle mode, 50 mA

Peaks: 1. Lithium 0.05 mg/L (ppm) 2. Sodium 0.20 3. Ammonium 0.42 4. Ethanolamine 0.50 5. Methylamine 0.50 6. Potassium 0.50 7. Dimethylamine 1.00 8. 5-Amino-1-pentanol 2.00 9. Morpholine 2.00 10. Trimethylamine 1.50 11. Magnesium 0.25 12. Calcium 0.50

17906

0 5 10 15 20 25 30 35

Minutes

2.3

µS

0.1

1 2

3

45 6

7 89

10

11 12

Minutes

17908

Column: IonPac CS16 (3 × 250 mm)Eluent: 6 mM Methanesulfonic acid, gradient from 0 to 13 min to 8 mM Methanesulfonic acid, gradient from 13 to 25 min to 55 mM Methanesulfonic acidEluent Source: EG40Flow Rate: 0.5 mL/minInj. Volume: 25 LTemperature: 60 °C Detection: Suppressed conductivity, CSRS-ULTRA 2 mm, AutoSuppression recycle mode, 100 mA

Peaks: 1. Lithium 0.1 mg/L (ppm) 2. Sodium 0.4 3. Ammonium 0.5 4. Ethanolamine 0.5 5. Diethanolamine 1.0 6. Potassium 1.0 7. Triethanolamine 18.0 8. Magnesium 0.5 9. Calcium 1.0

0 5 10 15 20 25

1 2 3

45

6

7

8

9

4.5

µS

0.1

Figure 9. The IonPac CS16 packing permits the injection of acidic samples without pH adjustment.

Long-Term DurabilityTheIonPacCS16packingensures

long-termcolumnstabilityandtrouble-freeoperation.Thecolumniscompat-iblewithacidiceluentsandsamples.Performanceofthehigh-capacityCS16columndoesnotdeterioratewiththeinjectionofacidicsamplesuptoap-proximately100mNhydroniumionasillustratedinFigure9.AciddigestsorpreservedsamplescanbeinjectedwithoutpHadjustment.

Solvent-Compatible PackingTheIonPacCS16columnis100%

solvent-compatible.Addingaceto-nitriletotheeluentmodifiescolumnselectivityandenablestheelutionofnonpolaranalytesorcontaminantsfromthecolumn.Acetonitrilecanbeusedtoenhancesamplesolubility,reduceretentiontimes,andimprovethepeakshapesofhydrophobicamines.Timeandexpensecanbesavedbyeliminat-ingtime-consumingsampleprepara-tionsteps.Thisfeatureallowscomplexsamplematricestobeanalyzedwithminimalsamplepreparation.

System RecommendationsTheCS16columninthesup-

pressedconductivitymodeisrecom-mendedforusewiththeICS-2100orICS-5000ICSystemsequippedwithanEluentGenerator(EG).TheCS16canbeusedwitholderDionexICSystemsequippedwithanEGoranRFC-30ReagentFreeController.TheEluentGeneratorisusedtoautomaticallyproducemethanesulfonicacidgradientsfromdeionizedwater.

Minutes

50 mM H+

0 5 10 15 20 25

1.10

1 23

4 5 6

0 5 10 15 20 250

1.10

12

3

45 6

0 5 10 15 20 250

1.10

1

23

4

5 6

µS

µS

16174

µS

0

Column: IonPac CS16 (5 × 250 mm) Eluent: 30 mM Methanesulfonic acidFlow Rate: 1.0 mL/min Temperature: 40 °CInj. Volume: 25 L Detection: Suppressed conductivity, CSRS-ULTRA 4-mm, AutoSuppression recycle mode

Peaks: 1. Lithium 0.1 mg/L (ppm) 2. Sodium 0.4 3. Ammonium 0.5 4. Potassium 1.0 5. Magnesium 0.5 6. Calcium 1.0

100 mM H+

150 mM H+

SuppressorsForoptimumeaseofuseand

performance,theCS16columncanbeusedwiththeCSRS300Anion SuppressorortheCCES™300CationCapillaryElectrolyticSuppressor.

Cation Trap Columns WhenusingtheEluentGenerator

foreluentdelivery,aCR-CTCContinu-ouslyRegeneratedCationTrapColumnshouldbeinstalledbetweentheElu-entGeneratorcartridgeandtheEluentGeneratordegasmodule.Alternatively,aCTCCationTrapColumncanbeinstalledbetweenthegradientpumpandtheinjectionvalvetoremovecationiccontaminantsfromtheeluent.

Concentrator ColumnsFortraceanalysiswork,usethe

IonPacCG16guardcolumnwhenasinglepistonpumpsuchastheAXPpumpisusedforsampledelivery. UsetheTCC-LP1,TCC-ULP1,ortheTCC-XLP1CationConcentratorCol-umnwhenthesampleisdeliveredwithasyringeorwithanautosampler.Forconcentratorworkwitha0.4mmcapil-larycolumn,usetheCG16capillaryguardcolumnortheMCC-100concen-tratorcolumn.Fortwo-dimensionalIonChromatographymethodstodeterminetraceammoniumandamines,usetheMCC-200concentratorcolumn.

Printedonrecycledandrecyclablepaper.

CCESisatrademark,andAutoSuppression,IonPac,CationSelf-RegeneratingSuppressor,andCSRSareregisteredtrademarksofDionexCorporation.

PEEKisatrademarkofVictrexPLC.

SpecificationS

Dimensions: IonPacCS16CapillaryColumn: 0.5×250mm

IonPacCG16CapillaryGuard Column:0.5×50mm

CS16AnalyticalColumn:5 ×250mmand3×250mm

CG16GuardColumn:5×50mm and3×50mm

Maximum Operating Pressure: 4000psi(StandardandMicrobore)5000psi(Capillary)

Mobile Phase Compatibility: Acidiceluents,100%HPLCacetonitrile. Alcohols should be avoided.

Substrate Characteristics: BeadDiameter:5µm Crosslinking(%DVB):55%

Ion-Exchange Group: Graftedcarboxylicacid

Functional Group Characteristics: Mediumhydrophobic

Capacity (µeq/column): 84µeq/column(0.5×250mm) 17µeq/column(0.5×50mm) 8400µeq/column(5×250mm) 1700µeq/column(5×50mm) 3000µeq/column(3×250mm) 600µeq/column(3×50mm)

Column Construction: PEEK™with10-32threadedferrule-styleendfittings.Allcomponentsarenon-metallic.

ordering information

IntheU.S.,call1-800-346-6390orcontacttheDionexregionalofficenearestyou.OutsidetheU.S.,orderthroughyourlocalDionexofficeordistributor.Refertothefollowingpartnumbers.

Description Part Number

IonPac CS16 Columns IonPacCS16CapillaryColumn (0.5×250mm) ........................................................................................075401 IonPacCG16CapillaryGuardColumn (0.5×50mm) ..........................................................................................075402 IonPacCS16AnalyticalColumn (5×250mm) ...........................................................................................057573 IonPacCG16GuardColumn (5×50mm) .............................................................................................057574 IonPacCS16AnalyticalColumn (3×250mm) ...........................................................................................059596 IonPacCG16GuardColumn (3×50mm) .............................................................................................059595

Trap Columns CR-CTCIIContinuouslyRegeneratedCationTrapColumn ......................066262

CR-CTCContinuouslyRegeneratedCationTrapColumn(Capillary) (ForusewithCapillaryCationColumns) ................................................072079

CTC-1CationTrapColumn (9×24mm)forusewiththe5mmcolumns ..........................................040192

CTCCationTrapColumn (4×35mm)forusewiththe3mmcolumns ..........................................043132

Cation Concentrator Columns TCC-LP1LowPressureTraceCationConcentrator (4×35mm)forusewiththe3mmand5mmcolumns .........................046027

TCC-ULP1UltraTraceCationConcentrator-UltraLowPressure (5×23mm) .............................................................................................063783

TCC-XLP1UltraTraceCationConcentrator-ExtremelyLowPressure (6×16mm) .............................................................................................063889

MCC-100MonolithCationConcentratorColumn (0.5×80mm) ..........................................................................................075462

MCC-200MonolithCationConcentratorColumn (0.75×80mm) ........................................................................................075463

Passion. Power. Productivity. north america

U.S./Canada (847) 295-7500

South america

Brazil (55) 11 3731 5140

europe

Austria (43) 1 616 51 25 Benelux (31) 20 683 9768; (32) 3 353 4294 Denmark (45) 36 36 90 90 France (33) 1 39 30 01 10 Germany (49) 6126 991 0 Ireland (353) 1 644 0064 Italy (39) 02 51 62 1267 Sweden (46) 8 473 3380 Switzerland (41) 62 205 9966 United Kingdom (44) 1276 691722

asia pacific

Australia (61) 2 9420 5233 China (852) 2428 3282 India (91) 22 2764 2735 Japan (81) 6 6885 1213 Korea (82) 2 2653 2580 Singapore (65) 6289 1190 Taiwan (886) 2 8751 6655

dionex corporation

1228 Titan Way P.O. Box 3603 Sunnyvale, CA 94088-3603 (408) 737-0700 www.dionex.com LPN 1257-04 PDF 11/10

©2010 Dionex Corporation